T. Satyanarayana

• University of Delhi

Lignocellulosic cellulose serves as a key source for bioethanol production. The efficient conversion of cellulose relies on three major cellulase components. High cost of cellulases and the need for a single microbe that produces all cellulase components in the right proportion and quantities are a few challenges in bioethanol production from cellu...

The purpose of food processing is to produce better-quality foods having good organoleptic properties. For centuries, enzymes have been in use for food processing. In the last three decades, xylan-hydrolyzing enzymes have been explored for their utility in food processing industries. Xylanases find applications in the bioconversion of lignocellulos...

This book presents various biotechnological applications of the fungal systems in pharmaceuticals, nutraceuticals, textile industry, bioremediation, biofuel, and the production of biomolecules. It discusses the important role of fungal secondary metabolites in human welfare and nutrition. It explores fungi as the vital sources of novel substances w...

This book presents various biotechnological applications of the fungal systems in pharmaceuticals, nutraceuticals, textile industry, bioremediation, biofuel, and the production of biomolecules. It discusses the important role of fungal secondary metabolites in human welfare and nutrition. It explores fungi as the vital sources of novel substances w...

The GH3 β-glucosidase gene of Myceliophthora thermophila (MtBgl3c) has been cloned and heterologously expressed in E. coli for the first time. This study highlights the important characteristics of recombinant MtBgl3c (rMtBgl3c) which make it a promising candidate in industrial applications. Optimization of the production of rMtBgl3c led to 28,000...

Cellobiohydrolase (CBH) is one of the cellulases with a wide range of industrial applications; it plays a pivotal role in cellulose hydrolysis and thus in biofuel production. The structural and thermostability analysis of a CBHII of the thermophilic mold Myceliophthora thermophila (MtCel6A) had been carried out using various in-silico approaches. T...

Cellobiohydrolase (CBH) is one of the cellulases with a wide range of industrial applications; it plays a pivotal role in cellulose hydrolysis and thus in biofuel production. The structural and thermostability analysis of a CBHII of the thermophilic mold Myceliophthora thermophila (MtCel6A) had been carried out using various in-silico approaches. T...

Azo dyes, like Congo red (CR), are persistent contaminants that cause detrimental consequences on living organisms in various habitats. In the present study, a novel and facile one‐pot citric acid functionalized iron oxide magnetic nanoparticles (Fe3O4@CA NPs) through an electrochemical method are synthesized and characterized for adsorbing CR dye...

Thermophiles and hyperthermophiles are immensely useful in understanding the evolution of life, besides their utility in environmental and industrial biotechnology. Advancements in sequencing technologies have revolutionized the field of microbial genomics. The massive generation of data enhances the sequencing coverage multi-fold and allows to ana...

Twitter data analysis plays a vital role in disaster to identify the precious and non-precious tweets in the form of text and images. This real time information is required to take necessary action during the disaster times. In the existing method, the Stacked convolution method is used which is a combination of SVM classifier and KNN classifier as...

Glucoamylases hydrolyze α-1,4-glycosidic and α-1,6-glycosidic bonds in soluble as well as raw starches. Other names of the enzyme include amyloglucosidase and γ-amylase. Its structure is highly conserved among fungal species and produced by several fungal species. Glucoamylases are industrially significant enzymes where they are used in producing c...

A codon optimized cellobiohydrolase (CBH) encoding synthetic gene of 1188 bp from a thermophilic mold Myceliophthora thermophila (MtCel6A) was cloned and heterologously expressed in Escherichia coli for the first time. In silico analysis suggested that MtCel6A is a GH6 CBH and belongs to CBHII family, which is structurally similar to Cel6A of Humic...

Thermophilic fungi have been isolated from a great variety of natural and man-made environments. These fungi grow in simple media containing carbon and nitrogen sources and mineral salts. Polyamines are essential for their growth and synthesized in these moulds. The composition of lipids varies considerably, predominantly containing palmitic, oleic...

Cellulases play a promising role in the bioconversion of renewable lignocellulosic biomass into fermentable sugars which are subsequently fermented to biofuels and other value-added chemicals. Besides biofuel industries, they are also in huge demand in textile, detergent, and paper and pulp industries. Low titres of cellulase production and process...

Industrial Revolution has led to an unprecedented rise in carbon dioxide concentrations in the atmosphere. Among various methods available for carbon capture from the industrial emissions such as flue gas, carbonic anhydrase (CA)-based carbon capture techniques have been evolved and gained immense attention in the recent years. Carbonic anhydrase (...

Cellulases are the enzymes with diverse range of industrial applications. Cellulases degrade cellulose into monomeric glucose units by hydrolysing β-1,4-glycosidic bonds. There are three components of cellulases: a) endoglucanase, b) exoglucanase and c) β-glucosidase which act synergistically in cellulose bioconversion. The cellulases are the third...

Across the world, plant products, especially of cereals and legumes, are the major staple food and feed sources for humans and animals. The seeds and grains contain phytic acid, the major repository form of organic phosphorus, which is not digestible by monogastrics and acts as an antinutrient. Phytases are a group of hydrolytic enzymes that cataly...

The book comprises state-of-the-art scientific reviews on carbon management strategies in response to climate change. It provides in-depth information on topics relating to recent advances in carbon capture technology and its reuse in value added products. It features contributions of leading scientists and technocrats on topics including climate c...

Phytases, a class of phosphatases, catalyse the stepwise removal of soluble phosphate from phytates. Plants contain phytates, which are a major organic form of phosphorus. Phytates act as an antinutrient and is not utilizable by monogastrics. Human and animal diets can therefore be supplemented with phytases to ameliorate their nutritional status....

Xylanolytic enzymes have extensive applications in paper, food, and feed, pharmaceutical, and biofuel industries. These industries demand xylanases that are functional under extreme conditions, such as high temperature, acidic/alkaline pH, and others, which are prevailing in bioprocessing industries. Despite the availability of several xylan-hydrol...

Background: Microbe-mediated redox transformation of arsenic (As) leading to its mobilization has become a serious environmental concern in various subsurface ecosystems especially within the alluvial aquifers. However, detailed taxonomic and eco-physiological attributes of indigenous bacteria from As impacted aquifer of Brahmaputra river basin ha...

The bioactive form of thermostable and alkali stable pectinase of Bacillus pumilus dcsr1 is a homodimer of the molecular mass of 60 kDa with a pI of 4.6. The enzyme is optimally active at 50 °C and pH 10.5, and its Michaelis constant (Km), maximum rate of reaction (Vmax), activation energy (Ea), and temperature quotient (Q10) values (for citrus pec...

Lignocellulosic biomass is a renewable and sustainable energy source. Cellulases are the enzymes that cleave β-1, 4-glycosidic linkages in cellulose to liberate sugars that can be fermented to ethanol, butanol, and other products. Low enzyme activity and yield, and thermostability are, however, some of the limitations posing hurdles in saccharifica...

Bioethanol as a biofuel has received significant attention in the recent years. This is expected to provide a solution to the heavy reliance on petroleum based products for energy security, adverse effect on the environment and climate change due to emissions of vehicles. India, being an agriculture-based economy, produces huge quantities of agricu...

Heterogenous nature of xylan leads to the multiplicity in xylanolytic enzymes for its complete degradation, where β-1,4-endoxylanases and β-xylosidases play a key role due to their direct action on glycosidic linkages of xylan backbone. α-Glucuronidase (EC 3.2.1.139), α-L-arabinofuranosidase (EC 3.2.1.99), acetyl xylan esterase (EC 3.1.1.6), and ly...

Cellulose is a renewable carbon source, which is an abundant natural biopolymer present in agricultural and forest residues. A great variety of prokaryotic and eukaryotic microbes are known to produce cellulose-degrading enzymes. Besides well-known cellobiohydrolase, endoglucanase and β-xylosidase, other enzymes such as lytic polysaccharide monooxy...

Thermophilic fungi inhabit a great variety of ecosystems such as soils, composts and several others. They have also been isolated from non-thermogenic environments. Both morphological and molecular approaches have been employed in identifying them. These fungi degrade a large number of lignocellulosic and other biomasses by producing a wide range o...

This book discusses microbial diversity in various habitats and environments, its role in ecosystem maintenance, and its potential applications (e.g. biofertilizers, biocatalysts, antibiotics, other bioactive compounds, exopolysaccharides etc.). The respective chapters, all contributed by renowned experts, offer cutting-edge information in the fiel...

This volume comprehensively reviews recent advances in our understanding of the diversity of microbes in various types of terrestrial ecosystems, such as caves, deserts and cultivated fields. It is written by leading experts, and highlights the culturable microbes identified using conventional approaches, as well as non-culturable ones unveiled wit...

The book provides an introduction to the basics of fungi, discussing various types ranging from edible mushrooms to Neurospora – a model system for genetics and epigenetics. After addressing the classification and biodiversity of fungi, and fungi in different ecological niches, it describes the latest applications of fungi, their role in sustainabl...

Amylopullulanases are endoacting bifunctional enzymes capable of hydrolyzing α-1,4- and α-1,6-glycosidic linkages in starch, amylose, pullulan, amylopectin and related oligosaccharides. These enzymes possess single or dual active site(s) for cleaving α-1,4- and α-1,6-glycosidic bonds; the former are called amylopullulanases, and the latter, α-amyla...

Most of the extracellular enzymes of acidophilic bacteria and archaea are stable at acidic pH with a relatively high thermostability. There is, however, a dearth of information on their acid stability. Although several theories have been postulated, the adaptation of acidophilic proteins to low pH has not been explained convincingly. This review hi...

Molecular and eco-physiological characterization of arsenic (As)-transforming and hydrocarbon-utilizing Achromobacter type strain KAs 3–5T has been investigated in order to gain an insight into As-geomicrobiology in the contaminated groundwater. The bacterium is isolated from As-rich groundwater of West Bengal, India. Comparative 16S rRNA gene sequ...

Reductive transformation of toxic arsenic (As) species by As reducing bacteria (AsRB) is a key process in As-biogeochemical-cycling within the subsurface aquifer environment. In this study, we have characterized a Gram-stain-negative, non-spore-forming, rod-shaped As reducing bacterium designated KAs 5-3T, isolated from highly As-contaminated groun...

A, Microbial culture collection (MCC) deposition certificate of strain KAs 5-3T. B, Microbial type culture collection (MTCC) deposition certificate of strain KAs 5-3T. C, Belgian coordinated culture collection (BCCM) deposition certificate of strain KAs 5-3T. (PDF)

Table A, Details of PCR primers used for 16S rRNA, MLSA, and functional gene analysis. Table B, GC mol % and dGC mol % (deviation) from their respective genomic GC content of arsC and narG sequences (phylogenetically closest) as a measure of horizontal gene transfer event. Figure A, Phylogenetic tree involving 16S rRNA gene sequences of strain KAs...

Recombinant α-carbonic anhydrase of the polyextremophilic bacterium Bacillus halodurans TSLV1 (rBhCA) has been produced extracellularly in active form in Pichia pastoris under methanol inducible (AOX1) as well as constitutive (GAP) promoters. A marked improvement in rBhCA production was achieved by developing a P. pastoris recombinant that produces...

Utilization of extremophilic microorganisms in mitigation of climate change due to global warming

Most of cereal and legume seeds and their products contain 1–2% phytic acid that represents around 60% of the total phosphorus content. A large portion of phytic acid in seeds is in the form of salts known as phytates. The phytic acid-bound phosphorus (myoinositol 1,2,3,4,5, 6-hexakis dihydrogen phosphate) is poorly available to monogastrics. There...

This book explores the developments in important aspects of fungi related to the environment, industrial mycology, microbiology, biotechnology, and agriculture. It discusses at length both basic and applied aspects of fungi and provides up-to-date laboratory-based data. Of the estimated three million species of fungi on Earth, according to Hawkswor...

Phytic acid is the major storage form of phosphorus that accounts for 50-80 % in most of foods and feeds of plant origin. Monogastric animals such as chicks and humans are unable to hydrolyze phytates present in plant-based foods due to lack of phytase that hydrolases phytic acid into inositol and inorganic phosphate. Undigested phytic acid and phy...

In this study, two xylanase genes (GH10 and GH11) derived from Malbranchea cinnamomea, designated as XYN10A_MALCI and XYN11A_MALCI, respectively, were expressed in Pichia pastoris X33. The maximum level of xylanase expression was found to be 24.3U/ml for rXYN10A_MALCI and 573.32U/ml for rXYN11A_MALCI. The purified recombinant rXYN11A_MALCI was stab...

All the leading cities in the world are slowly becoming inhospitable for human life with global warming playing havoc with the living conditions. Biomineralization of carbon dioxide using carbonic anhydrase (CA) is one of the most economical methods for mitigating global warming. The burning of fossil fuels results in the emission of large quantiti...

Codon adaptation index (CAI) of a 1263 bp long endoglucanase encoding gene from the thermophilic mould Myceliopthora thermophile BJA has been improved from 0.44 to 0.76 by in vitro gene synthesis. The codon optimized endoglucanase gene (Mt-egl) has been constitutively expressed in Pichia pastoris under the regulation of GAP promoter. Recombinant en...

Most of cereal and legume seeds and their by-products contain 1–2% phytic acid (myo-inositol-1,2,3,4,5,6-hexakis dihydrogen phosphate) that represents >60% of total phosphorus content in plant products. A large portion of phytic acid in various plant seeds is in the form of salts called phytates. The phytate phosphorus is scarcely available to mono...

This book brings together and updates the latest information on the diversity of yeasts, their molecular features and their applications in the welfare of mankind. Yeasts are eukaryotic microfungi widely found in natural environments, including those with extreme conditions such as low temperatures, low oxygen levels and low water availability. To...

The recombinant alkalistable and moderately thermostable bifunctional endoglucanase gene (BhCell-Xyl) of polyextremophilic bacterium Bacillus halodurans TSLV1 has been expressed in Pichia pastoris under constitutive GAP as well as inducible AOX promoters. A higher titre of recombinant BhCell-Xyl was attained after induction (4.8 U mL⁻¹) as compared...

Streptomyces badius DB-1 produces α-amylase extracellularly, and its production was enhanced 5.1-fold (from 9.47 ± 0.51 to 48.23 ± 1.45 U mL(-1)) due to optimization by one-variable-at-a-time and statistical approaches. Soluble starch emerged as the most influential factor that strongly affected enzyme production. The purified enzyme is a monomer w...

Aeribacillus pallidus TSHB1 polyextremophilic bacterium produces a γ-carbonic anhydrase (ApCA), which is a homotrimeric biocatalyst with a subunit molecular mass of 32 ± 2 kDa. The enzyme is stable in the pH range between 8.0 and 11.0 and thus alkali-stable and moderately thermostable with T1/2 values of 40 ± 1, 15 ± 1, and 8 ± 0.5 min at 60, 70, a...

Carbon capture storage and utilization (CCSU) can be a probable and efficient solution for mitigating global warming. It refers to the conversion and storage of CO2 in stable and usable forms. Carbon sequestration using carbonic anhydrase (CA) has attracted much attention in the recent years. Due to high temperature and CO2 content in flue gas emit...

Recombinant chimeric α-amylase (Ba-Gt-amy) has been produced extracellularly in Pichia pastoris under AOX promoter. Clones of P. pastoris with multiple gene copies have been generated by multiple transformations and post-transformational vector amplification, which led to 10.7-fold enhancement in α-amylase titre as compared to a clone with a copy o...

A novel arsenic (As)-resistant, arsenate-respiring, alkane-metabolizing bacterium KAs 5-22T, isolated from As-rich groundwater of West Bengal was characterized by physiological and genomic properties. Cells of strain KAs 5-22T were Gram-stain-negative, rod-shaped, motile, and facultative anaerobic. Growth occurred at optimum of pH 6.0–7.0, temperat...

The 3′-deleted amylopullulanase gene from the extreme thermophile Geobacillus thermoleovorans (Gt-apuΔC) was expressed extracellularly in Pichia pastoris under both methanol-inducible AOX1 and constitutive GAP promoters. The expression of the gene (Gt-apuΔC) was higher under GAP promoter (36.2 U ml−1, α-amylase; 33.5 U ml−1, pullulanase) than that...

The advent of green revolution or high input agrotechnologies have led to self-reliance in food production. Modern agriculture methods are getting increasingly dependent on the steady supply of synthetic inorganic fertilizers and pesticides, which are products of fossil fuels. There is an increasing concern about the excessive dependence on the sup...

Carbonic anhydrase (CA) based conversion of CO2 to CaCO3 has been identified as a green and economic strategy to sequester CO2 from flue gas and industrial emissions. The method is, however, cost-intensive as an efficient immobilization method for reusing the enzyme poses a major challenge. In this investigation, the recombinant α-carbonic anhydras...

For saccharifying starch in one step, a chimeric biocatalyst (Amy-Glu) was generated from engineered α-amylase (Ba-Gt-amy) of Bacillus acidicola and glucoamylase (Glu) gene of Aspergillus niger. In order to join two enzymes, a linker peptide of 25 amino acids was used. Chimeric Amy-Glu was expressed in E. coli. Glu is of 75 kDa, while Amy-Glu is of...

Functional genomics attempts to answer questions about the functions of DNA at the level of genes, RNA transcripts, and protein products. A key characteristic of functional genomics is the utilization of a genome-wide as well as a high-throughput technical approach combined with statistical or computational analysis to answer questions by making us...

Microbiology deals with the study of microorganisms and their interactions with biotic and abiotic components of the environment. Microbes are so small that they are not seen by the naked eye, and include bacteria and archaea, fungi, algae, protozoa, and viruses. Microbes are the main decomposers of organic matter and, therefore, influence all livi...

The annual sale of amylolytic enzymes is estimated to reach $2,238.4 million by 2018. Starch hydrolyzing enzymes find major applications in baking, alcohol, detergent and textile industries. Geobacillus thermoleovorans, an extremely thermophilic bacterium, is a well known producer of starch hydrolyzing enzymes. With the advent of high throughput ge...

The thermostable bifunctional CMCase and xylanase encoding gene (rBhcell-xyl) from Bacillus halodurans TSLV1 has been expressed in Escherichia coli. The recombinant E. coli produced rBhcell-xyl (CMCase 2272 and 910 U L⁻¹ xylanase). The rBhcell-xyl is a ~62-kDa monomeric protein with temperature and pH optima of 60 °C and 6.0 with T1/2 of 7.0 and 3....

The recombinant acidstable α-amylase (Ba-amy) of acidophilic bacterium Bacillus acidicola TSAS1 has been produced extracellularly using a combination of cloning (E. coli and P. pastoris) and physico-chemical treatment strategies. A total of 150,000 U/L of Ba-amy were attained under constitutive promoter in P. pastoris, which is 15-fold higher than...

Carbonic anhydrase (CA) was produced from the polyextremophilic (halotolerant, moderately thermophilic and alkaliphilic) bacterium Aeribacillus pallidus TSHB1 isolated from water and sediment samples of Choti Anhoni hot spring of Pipariya, Madhya Pradesh (India), is being reported to be suitable for carbon sequestration. Growth and CA production we...

Chitinase from the thermophilic mould Myceliopthora thermophila BJA (MtChit) is an acid tolerant, thermostable and organic solvent stable biocatalyst which does not require any metal ions for its activity. In order to produce high enzyme titres, reduce fermentation time and overcome the need for induction, this enzyme has been heterologously expres...

The emissions of CO2 into the atmosphere have been constantly rising due to anthropogenic activities, which have led to global warming and climate change. Among various methods proposed for mitigating CO2 levels in the atmosphere, carbonic anhydrase (CA)-mediated carbon sequestration represents a greener and safer approach to capture and convert it...

Industrial enzyme market has been projected to reach US$ 6.2 billion by 2020. Major reasons for continuous rise in the global sales of microbial enzymes are because of increase in the demand for consumer goods and biofuels. Among major industrial enzymes that find applications in baking, alcohol, detergent, and textile industries are α-amylases. Th...

Pullulan hydrolyzing enzymes are endoacting, classified based on the substrate specificity and hydrolysis products as pullulanases (type I and II) and pullulan hydrolases (type I, II and III). Pullulanases and pullulan hydrolase type I are produced by bacteria and archaea. Among bacteria, many mesophilic, thermophilic and hyperthermophilic bacteria...

Carbonic anhydrase (CA) is a biocatalyst that catalyzes the hydration of CO2 to bicarbonate and protons, thus useful in mitigating green house effect by sequestering CO2 from various point sources. An alkalistable and moderately thermostable α- carbonic anhydrase encoding gene (BhCA) from Bacillus halodurans TSLV1 has been cloned and expressed in E...

Acidophilic microorganisms are an ecologically and economically important group, which occur in acidic natural (solfataric fields, sulphuric pools) and man-made (eg. Acid mine drainage) environments. Acidophiles possess networked cellular adaptations for regulating intracellular pH. Several extracellular enzymes from acidophilic microbes are known...

The codon-optimized phytase gene of the thermophilic mold Sporotrichum thermophile (St-Phy) was expressed in Pichia pastoris. The recombinant P. pastoris harboring the phytase gene (rSt-Phy) yielded a high titer of extracellular phytase (480 ± 23 U/mL) on induction with methanol. The recombinant phytase production was ~40-fold higher than that of t...

The α-amylase (Ba-amy) of Bacillus acidicola was fused with DNA fragments encoding partial N- and C-terminal region of thermostable α-amylase gene of Geobacillus thermoleovorans (Gt-amy). The chimeric enzyme (Ba-Gt-amy) expressed in Escherichia coli displays marked increase in catalytic efficiency [K cat: 4 × 104 s−1 and K cat/K m: 5 × 104 mL−1 mg−...

Thermophilic molds thrive in a variety of natural habitats including soils, composts, wood chip piles, nesting materials of birds and other animals, municipal refuse and others, and ubiquitous in their distribution. These molds grow in simple media containing carbon and nitrogen sources and mineral salts. Polyamines are synthesized in these molds a...

The optimum values of the critical variables determined by the central composite design of response surface methodology (RSM) for maximum phytase production (1881.26 U g(-1) dry mouldy residue (DMR)) by Sporotrichum thermophile are 2.5 % Tween 80, 1.0 % yeast extract and 48 h of incubation period. Phytase production in the mixed substrate (sugarcan...

Sporotrichum thermophile produces very low titres of phytase (St-Phy) extracellularly, which is acidstable, thermostable, and protease insensitive with broad substrate specificity, and therefore, the gene encoding phytase (St-Phy) has been cloned and expressed in E. coli. The purified recombinant phytase (rSt-Phy) has the molecular mass of 55 kDa w...

Microbes belonging to the phylum Actinobacteria are prolific sources of antibiotics, clinically useful bioactive compounds and industrially important enzymes. The focus of the current review is on the diversity and potential applications of thermophilic and alkaliphilic actinobacteria, which are highly diverse in their taxonomy and morphology with...

The phytase of the yeast Pichia anomala (PPHY) is a suitable biocatalyst as a food and feed additive because of its adequate thermostability, acid stability, protease insensitivity and broad substrate spectrum. The cell-bound nature and low phytase titres are the main bottlenecks for its utility in food and feed industries. In this investigation, w...

The phytases, which hydrolyse inositol hexaphosphate (IP6), are used as animal feed supplements for ameliorating phosphorus availability and assimilation, and mitigating anti-nutrient effects of phytates. Phytases have been reported from several yeasts and fungi. The phytase titres attainable from the wild yeast and fungal strains are very low and,...

The phytase of the yeast Pichia anomala is a histidine acid phosphatase based on signature sequences and catalytic amino acids identified by site-directed mutagenesis. Among modulators, N-bromosuccinimide and butanedione inhibit phytase, while Ca(2+) and Ni(2+) stimulate slightly. Vanadate exhibits competitive inhibition of phytase, making it bifun...

Maltogenic amylase of Geobacillus thermoleovorans (Gt-MamyIII), which has the highest thermostability among bacterial maltogenic amylases, has been used as a model enzyme to understand the role of networked salt bridges in thermoadaptation. The role of intra-chain cross-domain salt bridge networks in the thermostabilization of maltogenic amylase of...

The far UV-CD spectroscopic analysis of the secondary structure in the temperature range between 30 and 90°C revealed a compact and thermally stable structure of C-terminal truncated amylopullulanase of Geobacillus thermoleovorans NP33 (gt-apuΔC) with a higher melting temperature [58°C] than G. thermoleovorans NP33 amylopullulanase (gt-apu) [50°C]...

Oligosaccharides and dietary fibres are non-digestible food ingredients that preferentially stimulate the growth of prebiotic Bifidobacterium and other lactic acid bacteria in the gastro-intestinal tract. Xylooligosaccharides (XOS) provide a plethora of health benefits and can be incorporated into several functional foods. In the recent times, ther...

In order to understand the role of N1 domain (1–257 aa) in the amylopullulanase (gt-apu) of the extremely thermophilic bacterium Geobacillus thermoleovorans NP33, N1 deletion construct (gt-apuΔN) has been generated and expressed in Escherichia coli. The truncated amylopullulanase (gt-apuΔN) exhibits similar pH and temperature optima like gt-apu, bu...

The recombinant Pichia pastoris harboring the endoxylanase gene (TSEV1xyl) of Bacillus halodurans TSEV1 yielded a high titer of extracellular xylanase (502±23Uml(-1)) on induction with methanol. The purified recombinant xylanase (TSEV1xyl) displayed optimal activity at 80°C and pH 9.0. The glycosylated recombinant xylanase exhibited higher thermost...