T. Pullaiah

• PhD
• Sri Krishnadevaraya University

Brachystelma volubile Hook.f. (Apocynaceae - Asclepiadoideae) has been rediscovered from near the type locality in the Kadapa district of Andhra Pradesh, after a lapse of about 130 years. A detailed description with illustrations is provided. Its current threat status has also been assessed according to the IUCN guide lines.

An efficient protocol was described for the rapid in vitro multiplication of an endangered medicinal plant, Caralluma bhupenderiana Sarkaria, via enhanced axillary bud proliferation from nodal explants collected from young shoots of six-months-old plant. The physiological effects of growth regulators [6- Benzyladenine (BA), kinetin (Kn), 2-Isopenty...

Plant regeneration through direct somatic embryogenesis in Ceropegia spiralis was achieved in the present investigation. Callus was obtained from nodes, internodes and thin cell layers (TCLs) of the plant. Explants were cultured on Murashige and Skoog (MS) medium supplemented with N-6-benzyl amino purine (BAP) with different concentrations of auxin...

Shoot multiplication of M. brunoniana Wight & Arn. was achieved from the nodal explants of mature plants using MS with different concentrations and combina‐ tions of growth regulators. Maximum explant response and highest number of shoots per explant was obtained on MS medium fortified with 1.0 mg/l BAP. The highest degree of shoot proliferation wa...

6 benzyladenine (BA) was best for axillary bud proliferation inducing a mean of 5.51 shoots per node. Excision and culture of the nodal segments from the in vitro shoots on fresh medium with same concentration of BA facilitated development of more than 5 shoots per node. Subsequent subculture slightly enhanced up to third subcultures and decreased...

A procedure for in vitro propagation of pharmaceutically valuable varieties of Caralluma adscendens from nodal explant, is described. The highest shoot multiplication with 80% frequency was achieved within one month on Murashige and Skoog’s medium supplemented with 8.87 μM BA. Shoot multiplication occurred in subsequent subcultures in culture bottl...

Tropical and Subtropical Agroecosystems, Vol. 10, Núm. 3, septiembre-diciembre, 2009, pp. 313-335 Universidad Autónoma de Yucatán México ¿Cómo citar? Número completo Más información del artículo Página de la revista Tropical and Subtropical Agroecosystems ccastro@uady.mx Universidad Autónoma de Yucatán México www.redalyc.org Proyecto académico sin...

The members of Euphorbiaceae are valuable source of different kinds of useful products like dyes, edible tubers, oil crops, furniture, agricultural implements, ornamental plants, pharmacological products, rubber, timber and aesthetic items. Micropropagation is an alternative mean of propagation that can be employed in conservation of the flora in r...

An efficient protocol has been developed for in vitro propagation of Caralluma sarkariae Lavranos & Frandsen via mature internodal derived callus. Optimal callus was developed for regeneration from mature internodal explants on Murashige and Skoog (MS) basal medium supplemented with various concentrations of auxins. Maximum number of shoots were re...

Pollination in the species of Ceropegia juncea Wight (Apocynaceae: Ceropegiae) in Southern India is discussed based on observation from ex situ grown plants. The study confirmed pollinators are small, mostly female Dipteran flies, ca 3 mm long, which carry pollinaria on the proboscis. The most important factors for an insect to effect pollination a...

The Eastern Ghats, one of the nine floristic zones in India, are located between 11 30' E longitude in a North-East to South-West strike. The Eastern Ghats are spread over three states of India, namely Orissa, Andhra Pradesh and Tamil Nadu. It covers an area of about 75,000 sq.km. with an average width of 200 km in the north and 100 km in the south...

The members of Euphorbiaceae are valuable source of different kinds of useful products like dyes, edible tubers, oil crops, furniture, agricultural implements, ornamental plants, pharmacological products, rubber, timber and aesthetic items. Micropropagation is an alternative mean of propagation that can be employed in conservation of the flora in r...

Callus cultures were established from seedling explants of Pergularia daemia (Forsk) Chiov on Murashige and Skoog (MS) medium supplemented with different concentrations of auxins. Optimal callus developed from leaf explants on MS medium supplemented with 2,4-D (2 mg l−1) + 2iP (0.1 mg l−1), was used for morphogenesis. Adventitious shoots were regen...

Shoot multiplication of Bupleurum distichophyllum was achieved from the nodal and shoot tip explants of mature plants using MS with different concentrations and combinations of growth regulators. Maximum explant response was from axillary shoots and the highest number of shoots per explant was obtained on MS fortified with 1.0 mg/l BAP. The highest...

Andhra Pradesh state comprises of mainly four vegetation types, the forest, along the coast, in aquatic zones and waste lands and weed vegetation. They constitute a total of 2601 plant species belonging to 1035 genera and 173 families. A total of 19 Rare, Endangered and Threatened plants are encountered in this region. Many plants that are endemic...

A protocol for in vitro propagation of Hydrocotyle conferta Wight (Apiaceae) through axillary bud multiplication was established. Murashige and Skoog (MS) medium with 6.66 N6-benzyladenine (BA) and 5.37 μM α-naphthalenacetic acid (NAA) was best suited for axillary bud multiplication including a mean of 21 shoots/node. Excision and culture of the no...

The present investigation deals with rapid micropropagation of Cassia siamea Lam. belonging to the family Caesalpiniaceae. This protocol was developed for multiplication by using shoot tip, cotyledonary node, and nodal explants derived from seedlings grown in vitro. Explants were cultured on MS, B 5 and MS macro salts + B5 micro salts medium supple...

The present study was conducted to establish a protocol for rapid propagation of Pergularia daemia (Asclepiadaceae) through different aseptic seedling explants. 40 days old aseptic seedlings grown on half strength MS medium were used as expiant. All the expiants were cultured on MS medium containing different concentration of cytokinins alone. MS m...

An efficient in vitro propagation of an endangered medicinal plant Vanasushava pedata (Apiaceae) by axillary shoot proliferation from nodal segments of mature plants was designed. The medium type and growth regulators markedly influenced in vitro regeneration of V. pedata. An in vitro plantlet production system has been investigated on MS with the...

The Eastern Ghats, one of the nine Floristic Zones in India. Eastern Ghats are spread over three States of India, namely Orissa, Andhra Pradesh and Tamil Nadu. The hilly areas of Eastern Ghats in Andhra Pradesh are mainly inhabited by 33 aboriginal/tribal communities, who still practice the age old traditional medicines and their application. There...

Two new varieties of Caralluma stalagmifera C. E. C. Fisch. (Asclepiadaceae), viz.: C stalagmifera var. Iongipetala and C stalagmifera var. intermedia, are described from Peninsular India. Identification keys for the three varieties are provided.

Eastern Ghats are a discontinuous chain of hill ranges running parallel to the east coast of peninsular India, extending through parts of four states; Orissa, Andhra Pradesh Karnataka and Tamil Nadu. Eastern Ghats are part of Deccan peninsula biogeographic zone supporting diverse dry tropical forests comprising of all plant life forms. Lianas (wood...

abstract included

Aglaia almeidai Sandhyarani, Sriramamurthy et Pullaiah Nom Nov.

Polyembryony in Rhynchosia hirta (Andr.) Meikle & Verdc.

Orchids constitute an order of royalty in the world of ornamentals for their extremely beautiful flowers. In fact orchids produce a large number of seeds, but very few (<5%) of them germinate under natural conditions because the seeds are non-endospermic, minute and require a mycorrhizal association. Propagation of orchids is a time consuming and t...

Characterization, Conservation and Utilization of Plant of Plant Genetic Resources for Future Food, Agriculture and Medicine

Wild relatives and related species of cultivated crop plants of Eastern Ghats, India. Recent trends in plant Sciences. Pullaiah et. al. ed. Page no 96-103; Regency Publications, New Delhi.

The antimicrobial activity of petroleum ether, benzene, chloroform, ethyl acetate and methanol extracts of Decalepis hamiltonii Wight and Arn. (Asclepiadaceae) against 15 different food-related microorganisms was evaluated. Among the solvent extracts, methanolic and petroleum ether extracts are the most active exhibiting growth inhibition of 11 and...

Floristic analysis of Andhra Pradesh, India

Hypericum gaitii Haines - A new record for Southern Peninsular India.

Somatic embryogenesis by the addition of heavy metals like Zn and Cu individually and in combination with cadmium was studied at different levels of these metals in MS media supplemented with 10 mg/l NAA. Absence of these two micro essential elements (Zn & Cu), the media induced somatic embryos. The experiment was carried out to study the induction...

Cotyledonary segments cultured on MS medium fortified with various combinations of auxins like Dicamba, IAA, IBA and NAA produced compact brown callus, which later on developed into greenish white shoot buds. These shoot buds on transferring to the shoot induction medium produced 20-25 shoots. Somatic embryogenesis of Mundulea sericea was achieved...

Abnormal flowering in Tecoma stans

The antimicrobial activity of petroleum ether, benzene, chloroform, ethyl acetate and methanol extracts of Decalepis hamiltonii Wight and Arn. (Asclepiadaceae) against 15 different food-related microorganisms was evaluated. Among the solvent extracts, methanolic and petroleum ether extracts are the most active exhibiting growth inhibition of 11 and...

In vitro regeneration protocol was developed for multiplication of Cryptolepis buchanani by using shoot tip, cotyledonary node and nodal explants derived from seedlings grown in vitro. The best response was achieved with nodal explants. Cultures were established placing the nodal explants on Murashige and Skoog's (MS; 1962) medium supplemented with...

The antimicrobial activity of petroleum ether, benzene, chloroform, ethylacetate and methanol extracts of Decalepis hamiltonii Wight and Arn. (Asclepiadaceae) against 15 different food-related microorganisms was evaluated. Among the solvent extracts, methanolic and petroleum ether extracts are the most active exhibiting growth inhibition of 11 and...

Medicinal plants used by the tribals of Nallamalais, Eastern Ghats of Andhra Pradesh, India

A protocol for plant regeneration from root segments excised from aseptic seedlings of Hemidesmus indicus R.Br. (Asclepiadaceae) has been developed. Excised root segments were placed horizontally on Murashige & Skoog's medium supplemented with 2% sucrose and various auxins either alone or in combination with cytokinins. Auxins or cytokinin individu...

Additions to the Flora of Anantapur District. Andhra Pradesh.

Trees and forests are among the most bountiful and versatile renewable resources gifted to mankind by nature. Tree wealth plays a vital role in the life and economy of many tribespeople of Andhra Pradesh, India. The plains and hill regions of the State are inhabited by a large number of tribespeople. They have learnt to utilise the tree products fo...

The essential oils of Chukrasia tabularis and Melia dubia were tested for their antimicrobial activity against ten different pathogenic microorganisms responsible for human pathologies using standard antimicrobial assays. Chukrasia tabularis leaf oil exhibited strong antibiotic activities against Proteus vulgaris and Fusarium oxysporum and did not...

The leaf oil of Chukrasia tabularis Adr. Juss. (Meliaceae) has been analyzed by GC/MS. The oil consisted of oxygenated monoterpenes (42.8%) and phenyl propanoids (25.2%). The oxygenated monoterpenes consisted chiefly of carvacrol (26.4%), thymol (5.2%) and borneol (3.0%), while the major phenyl propanoids characterized were (E)-methyl isoeugenol (1...

Vegetative propagation of Gymnema sylvestre R.Br. through stem cuttings.

A method for vegetative propagation of Gymnema sylvestre R.Br. through stem cuttings has been developed. Stem cuttings were subjected to different kinds of auxins in different concentrations for two minutes. Indole 3-butyric acid (2000 ppm) was significantly better than other treatments by inducing rooting in 85% of the cuttings with average root n...

Dye yielding plants of Andhra Pradesh, India

Dye yielding plants of Andhra Pradesh, India. Journal of Economic and Taxonomic Botany

Hydrodistillation of Decalepis hamiltonii roots yielded an essential oil (0.33% v/w) that contained 2-hydroxy-4-methoxybenzaldehyde (37.45%), 2-hydroxybenzaldehyde (31.01%), 4-O-methylresorcylaldehyde (9.12%), benzyl alcohol (3.16%), and alpha-atlantone (2.06%) as major constituents, with aromatic aldehydes constituting the main fraction of this ro...

In vitro propagation of Cynanchm callialtum Buch.- Ham ex Wight.

Flora of Eastern Ghats, Hill ranges of South East India

Flora of Eastern Ghats, Hill Ranges of South East India, Vol -2 Leguminosae ( Fabaceae)

Flora of Eastern Ghats, Hill Ranges of South East India Vol : 2

Alysicarpus ovalifolia A New record for the Eastern Ghats of Peninsular India.

The leaf essential oil of Melia dubia Cav. (Meliaceae) has been studied by GC–MS. The leaf essential oil consists chiefly of monoterpenes (35.71%) and oxygenated monoterpenes (27.98%), accompanied by a relatively much smaller amount of alkanes (11.17%), sesquiterpene hydrocarbons (9.26%) and phenylpropanoids (3.90%). The monoterpene camphene occurs...

Epidermal studies in some members of the family Oleaceae.

Successive petroleum ether, benzene, chloroform, ethyl acetate and methanol extracts of Chukrasia tabularis leaves were tested for their phytochemical constituents, antibacterial and antifungal activity. The ethyl acetate and methanol extracts were found to be most effective against most of the tested organisms.

Flora of Eastern Ghats:Hill ranges of south Eastern India VOl. 2

Genus Dalbergia L.f. (Leguminosae: Faboideae) in Eastern Ghats,

A contribution to the Flora of Eastern Ghats, India Genus Rhynchosia Lour. (Faboideae - Fabaceae)

The present study on 17 species of Crotalaria L. has shown that the genus exhibits four types of stomata i.e. anisocytic, anomocytic, paracytic and diacytic. The data on morphology of epidermal cell walls, nature and number of epidermal cells per unit area, stomatal frequency, and stomatal index are useful in delineating the species. The stomatal i...

The embryology of Clitoria ternatea was studied. Anthers contain four sporangia. The anther wall comprises an epidermis, an endothecium, a middle layer and a glandular tapetum. Microspore tetrads are tetrahedral and pollen grains are shed at the 2-cell stage. The ovule is campylotropous, bitegmic and crassinucellate. The micropyle is formed by both...

Rhynchosia hainesiana Satyan. et Thoth. (Fabaceae)- A new record for Southern Peninsular India

Native Phytotherapay for fever & malaria from Kurnool district, Andhra Pradesh

The present paper deals with the ethno-medico-botany of Chenchus of Mahaboobnagar district, Andhra Pradesh. About fourty four plants are enumerated with knowledge of the tribals for their medicinal uses in curing different diseases and ailments.

Occurrence of Twin seedlings in Ceropegia bulbosa Roxb. The Indian Forester

The growth and axillary branching was enhanced in bud cultures and seed cultures. An optimal in vitro propagation method was presented which can be used for rapid multiplication. In vitro raised shoots were excised and rooted successfully.

Embryology of Teramnus labialis (L.f.) Spreng. has been studied. The anthers are tetrasporangiate; it's wall development follows the Dicotyledonous type and consists of epidermis, endothecium, middle layer and the tapetum. Pollen tetrads are tetrahedral and decussate. Pollen grains are shed at the 2-celled stage. The ovule is campylotropous, bitegm...

The genus Desmodium Desv. (Faboideae) in Eastern Ghats, India – A systematic survey.

A Taxonomic account of the Genus Indigofera L. in Eastern Ghats, India.

Potentional Resources of Leguminosae in Eastern Ghats, India

The Genus Crotalaria L. In Eastern Ghats, India.

and 1998. Polyembryony in Strychnos potatorum L.

The paper deals with 50 selected species of wild plants used as medicine by tribals, as a result of an ethnobotanical survey carried out during 1994- 6 in the forest of Nallamalais, Prakasan district of Andhra Pradesh, a State of peninsular India.

Medak, one of the ten districts of Telangana region of erstwhile Andhra Pradesh (Presently in Tenagana State) was floristically explored for a period of 4 years during 1991-1995. A total of 702 species belonging to 410 genera and 118 families were recorded from the district.

Non -Timber Forest Resources in the economy of tribals of Nallamalis. Journal of Non Timber Forest Products

An ethnobotanical survey was carried out in Nallamalai hill ranges of Andhra Pradesh, India. Chenchus, Sugalis, Yanadis and Yerukalas are the main tribes inhabiting these hill ranges. This paper deals with the non-timber forest resources ex­ploited by the tribals of Nallamalai forest. These resources include gums, roots, bark, leaves, flowers, frui...

Galactia tenuiflora (Kelin ex Willd.) Wight & Arn. var. minor Baker (Faboideae). A New record for the Eastern Ghats of Peninsular India. Geobios new reports

Anthers contain four sporangia. The anther wall comprises of epidermis, endothecium, one middle layer and glandular tapetum. Pollen tetrads are tetrahedral, pollen grains are shed at two-celled stage. The ovule is campylotropous, bitegmic and crassinucellate. Megaspore tetrad is linear while embryo sac development is of the Polygonum type. Endosper...

Dalbergia rubiginosa Roxb. (Fabaceae) A New record for Andhra Pradesh

Dalbergia rubiginosa Roxb. (Fabaceae) A New record for Andhra Pradesh.

Wendlandia heynei (Roem. & Schult) Santhapau and Merchant. A new record for Andhra Pradesh

Embryology of Digitaria bicornis and D. ciliaris has been studied. The anthers are tetrasporangiate. The anther wall comprises of epidermis, endothecium, middle layer and glandular tapetum. Endothecium develops fibrous thickenings at 3-celled pollen grain stage in D. bicornis and at 1-nucleate pollen grain stage in D. ciliaris. Meiosis in pollen mo...

The occurrence of Flemingia nana Roxb. (Fabaceae) in Andhra Pradesh

Nizamabad, one of the 10 districts of Telangana region of erstwhile Andhra Pradesh State (currently in Telangana State) recorded with 708 species of vascular plants in explorations held during 1987-90. These species belongs to 436 genera and 123 families. In this book of 452 pages, introduction, past and present work on flora of the distrcit, flori...

The anthers are tetrasporangiate. The anther wall comprises epidermis, fibrous endothecium, middle layer and tapetal layer. The tapetum is of the Glandular type and its cells remain uninucleate. Meiosis in pollen mother cells is normal and simultaneous cytokinesis leads to the formation of tetrahedral and decussate microspore tetrads. The pollen gr...