T. K. Mohanty

T. K. Mohanty
National Dairy Research Institute | NDRI · Artificial Breeding Research Center

BSc Chemistry (Hons) B.V. Sc (AH) M.V.Sc. Ph.D. (Vet. Gynae. and Reproduction )

About

384
Publications
107,488
Reads
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2,232
Citations
Additional affiliations
May 1994 - May 2000
National Research Centre on Yak
Position
  • Researcher
Description
  • Arunachal Pradesh
Education
August 1981 - July 1983
MPC College, Baripada; Utkal University
Field of study
  • Chemistry

Publications

Publications (384)
Article
Estrus detection is a major problem in buffaloes because of the poor expression of estrus signs leading to low reproductive efficiency. Salivary transcripts analysis is a promising tool to identify biomarkers; therefore, the present study was carried out to evaluate their potential as estrus biomarkers. The levels of HSD17B1, INHBA, HSPA1A, TES tra...
Article
Full-text available
The research was based on a survey done in 2019 across Odisha’s eastern and southern coastal plain zones to assess sustainability among dairy farms based on various scientific management practices adopted by the farmers. The sustainability index was constructed using primary data acquired through a random sampling approach from 120 dairy households...
Article
The productivity of dairy animals in India needs to be improved to feed the growing population in the decades ahead. Sperm sexing offer a promising breeding strategy to meet the increased demand for food production and nutritional protection. Application of sexed sperm in the breeding of indigenous cattle and buffalo would help to protect endangere...
Chapter
The production of In-vitro embryos is a reproductive biotechnological technique that has great potential to speed up genetic improvement in cattle and is also an important research tool for livestock farming. In-vitro embryo production is a procedure of production of embryos artificially in a laboratory by three biological steps i.e. in vitro m...
Chapter
Laparoscopic ovum pick-up (LOPU) together with other reproductive biotechnologies in sheep possesses the capabilities to accelerate the genetic gain as well as reduce generation interval. It is a highly efficient and less invasive technique for the collection of the oocyte from the follicles of live animals. Oocytes from prepubertal animals can...
Preprint
Sperm mRNA transcriptional profiling can be used to evaluate the fertility of breeding bulls. This study aimed to compare the modified RNA isolation methods for higher RNA yield and quality from freshly ejaculated sperm of cattle and buffalo bull for further transcriptome analysis. Ten fresh ejaculates from each Sahiwal (n = 10 bulls x 10 ejaculate...
Article
Pyometra (pus in the uterus) is a common disease entity in intact female dogs during the luteal phase. Progesterone appears to be a crucial component in the pathogenesis of pyometra. Pyometra in canines results from hormonal imbalance; sequelae of cystic endometrial hyperplasia. Although the etiopathology of its primary aspect is unknown; however,...
Research
Full-text available
Accurate determination of estrus is essentially required for efficient reproduction management of farm animals. Buffalo is a shy breeder and does not manifest overt signs of estrus that make estrus detection difficult resulting in a poor conception rate. Therefore, identifying estrus biomarkers in easily accessible biofluid such as saliva is of utm...
Article
The present study was aimed to evaluate the effect of over dilution of semen with tris extender on motion and functional attributes of bull sperm post‐thaw. Ejaculates (n = 24; mass motility ≥3+) were collected from bulls (n = 4) using artificial vagina, diluted to 20, 15, 10 and 5 million spermatozoa/0.25 ml, and cryopreserved. The results reveale...
Article
Almost all species at the time of origin were seasonal breeders for assurance of feed to their young ones for better survival. But as domestication started this phenomenon is no more applicable for most of the species. Goats of the temperate regions are still seasonal breeders. Period of sexual quiescence can be minimized by different strategies li...
Conference Paper
Full-text available
Estrus detection is a major problem in buffaloes because of poor expression of estrus signs and higher incidences of silent estrus especially during summer season. Salivary transcript analysis is a noninvasive and promising tool for biomarker discovery. Hence, present study was carried out to evaluate the potential of salivary transcripts as estrus...
Conference Paper
Estrus detection is a major problem in buffaloes because of poor expression of estrus signs and higher incidences of silent estrus especially during summer season. Salivary transcript analysis is a noninvasive and promising tool for biomarker discovery. Hence, present study was carried out to evaluate the potential of salivary transcripts as estrus...
Preprint
Background : Estrus detection is a major problem in buffaloes because of the poor expression of estrus signs leading to low reproductive efficiency. Salivary transcripts analysis is a promising tool to identify biomarker; therefore, the present study was carried out to evaluate their potential as estrus biomarker. Methods : The levels of HSD17B1 ,...
Chapter
The epididymis is a complex organ where spermatozoa acquire motility and the ability to fertilize the egg. Ejaculated spermatozoa undergo several functional and physiological modifications by several factors, especially seminal plasma proteins that are constructed by the caudal epididymis and accessory sexual glands during ejaculation. During e...
Chapter
Full-text available
Heat stress may occur in those animals that are exposed to extreme environmental conditions i.e. 47 oC during summer months. Those cattle and buffaloes that have poor heat dissipation and have less sweet gland are more susceptible to heat stress. Higher environmental temperature with a higher temperature-humidity index is the major contributory...
Chapter
Full-text available
The variation in climatic variables like temperature, humidity and radiations were recognized as the potential hazards inflicting heat stress (HS) in all domestic livestock species including swine. India being a tropical country is among the most vulnerable area, since pathogens and epidemic diseases are widespread, climatic conditions are variable...
Chapter
Full-text available
The variation in climatic variables like temperature, humidity and radiations were recognized as the potential hazards inflicting heat stress (HS) in all domestic livestock species including swine. India being a tropical country is among the most vulnerable area, since pathogens and epidemic diseases are widespread, climatic conditions are variable...
Article
Full-text available
Iodine is anessential micronutrient that plays a crucial role in male reproduction (sexual behavior and semen production performance) by modulating thyroid function and the antioxidant status of the animal. Nonetheless, in Bos indicus bulls, a thorough evaluation of the effects of dietary iodine supplementation on antioxidant status, seminal qualit...
Article
Ticks are blood-sucking ectoparasites, a significant impediment to the dairy sector and implicated in diseases such as Babesiosis, Theileriosis, Anaplasmosis, etc., and deteriorate animal's health associated with welfare issues which have a drastic impact on the dairy farm economy and livestock productivity. Researchers's understanding of possible...
Article
Full-text available
Ticks are blood-sucking ectoparasites, a significant impediment to the dairy sector and implicated in diseases such as Babesiosis, Theileriosis, Anaplasmosis, etc., and deteriorate animal's health associated with welfare issues which have a drastic impact on the dairy farm economy and livestock productivity. Researchers's understanding of possible...
Article
Full-text available
Estrus identification in dairy animals is always a point of interest as it is well established that the pregnancy rate associated with heat detection rate; therefore in the present study 80 multiparous and 24 cyclic heifers of Sahiwal cows were monitored by digital thermal imaging DarviDTL007 camera during estrus and non-estrus period. The vulval,...
Article
Full-text available
The aim of study was to investigate the effect of parlour relocation on behaviour of lactating cows and to understand the effect of behaviour on post-adaptation milkability in dairy cows. Seventy-two crossbred cows initially milked in tandem milking parlour were relocated to herringbone parlour and observed for 45 milking sessions. Recording of beh...
Article
Full-text available
India ranked 1st in cow and buffalo population in the world with annual production of 187.75 MT of milk and 8.11 MT of meat, livestock contributes 4.1% in of total GDP. Even though, production is inadequate to fulfill the nutritional demands of population of the country. To generate bulk quantity of food products to fulfill the demands required to...
Article
Full-text available
In constrained availability of veterinarians and paramount study on the effectiveness of para-veterinary services, assessing the ability of these personnel vis-à-vis role proficiency and performance from stakeholder's perspective could act as impetus in reforming the animal healthcare and animal husbandry extension delivery system in India. The pre...
Article
Full-text available
In constrained availability of veterinarians and paramount study on the effectiveness of para-veterinary services, assessing the ability of these personnel vis-à-vis role proficiency and performance from stakeholder's perspective could act as impetus in reforming the animal healthcare and animal husbandry extension delivery system in India. The pre...
Article
Sperm cryopreservation allows sperms to be used for future in artificial insemination. It is the process by which sperm cells are brought to a zero level of metabolic state which allows its storage for future use. It also helps to make a lot much number of cows pregnant from a single ejaculate of bull. Though having so many advantages, cryopreserva...
Article
Mitochondria are vital organelles with a multifaceted role in cellular bioenergetics, biosynthesis, signaling and calcium homeostasis. During oxidative phosphorylation, sperm mitochondria generate reactive oxygen species (ROS) at physiological levels mediating signaling pathways essential for sperm fertilizing competence. Moreover, sperm subpopulat...
Article
Full-text available
Summer anoestrus is one of the major reproductive problems encountered in dairy buffaloes causing a huge economic loss to the buffalo breeders as well as the dairy industry. More than 50% of buffaloes were predisposed to summer anoestrus.Summer anoestrus is a multifactorial cause. Heat stress leading to decreased feed intake and hormonal disturbanc...
Article
Full-text available
Para-veterinary personnel are eyes and ears of public veterinary services which makes them an important part of animal health care and animal husbandry extension delivery system in India. With acute shortage of veterinarians and paravets, in order to meet various criteria such as availability, accessibility, acceptability, and affordability, assess...
Article
In the present study, the effect of cholesterol‐loaded cyclodextrin (CLC) on the quality of low sperm doses at post‐thaw was evaluated. Twenty four ejaculates (6 from each bull) were collected and split into eight aliquots. First four aliquots were diluted up to 20‐, 15‐, 10‐ and 5‐million sperm/0.25 ml, and remaining four were treated with CLC at...
Article
Full-text available
Retained placenta also known as retained foetal membrane or retained cleansing commonly occurs in animals. It occurs when the calf's side of the placenta (the fetal membranes) fails to separate from the mother's side. Separation of the membranes normally occurs after the calf is born. Retained placenta is usually defined as the failure to expel fet...
Article
Full-text available
Para-veterinary personnel are eyes and ears of public veterinary services which makes them an important part of animal health care and animal husbandry extension delivery system in India. With acute shortage of veterinarians and paravets, in order to meet various criteria such as availability, accessibility, acceptability, and affordability, assess...
Article
Full-text available
Animal biotechnology related to production has contributed to many improvements in agriculturally important traits in livestock. Although, artificial insemination was the first assisted reproductive technology used to enhance farm animal reproduction and propagate superior genetics, reproductive cloning and the development of transgenic animals are...
Article
Full-text available
Calving difficulty is associated with cow mortality, calf loss, veterinary and labor costs, and causes delayed return to estrus and lower conception rate. Dystocia is common in heifers, and the frequency decreases with the increasing age of the cow. Farmers need to consider both genetics and management factors in an attempt to reduce dystocia. Calf...
Article
Full-text available
In farm animals, several estrus synchronization programs are based on the use of hormones such as Progesterone, Prostaglandin F2α and their various combinations with other hormones such as Estrogen and Gonadotrophin Releasing Hormone (GnRH) are available. Besides the advantages and disadvantages of each system as well as the management capabilities...
Article
Full-text available
Ticks infestation considerably reduces milk yield of dairy animals due to blood sucking and poor feed intake. NDRI has conducted field trials on using polyharbal medication comprising neem and nirgundi leaves in eight villages of three districts in Haryana during 2019-2020. When the medication was used with 203 animals in 47 dairy farms, ticks were...
Chapter
Superovulation, embryo recovery and transfer, cryopreservation, and IVF are all well-established and effective techniques that are used in hundreds, if not thousands, of commercial businesses around the world. The method of extracting one or more embryos from a donor female's reproductive tract and transferring them to one or more recipient females...
Article
The present study compared the effect of mitochondria-targeted (Mitoquinone, MitoQ) and untargeted cytosolic antioxidant (Resveratrol, RESV) supplementation on lipid peroxidation (LPO) and in-vitro sperm functions of cryopreserved buffalo bull semen. To optimize additive’s concentration, sperm pellet obtained from twenty-four ejaculates was supplem...
Article
Full-text available
Postpartum anestrus is a major cause of infertility in buffaloes. The resumption of estrus in such animals depends on several factors such as nutrition, management, suckling, and so on. Season too affects estrus expression in buffaloes. Estrus expression during summer months is decreased in buffaloes which makes estrus detection difficult in them....
Article
Background: Improper estrus detection causes huge economic losses due to inaccurate time of insemination leading to poor conception rate. So, here pedometer proved to be a promising tool in defining optimal time of insemination,improving fertilization rates based on increased number of steps. The study is to determine the relationship between incre...
Article
Full-text available
The present study was undertaken to identify the difference in sperm kinematics in Murrah buffalo bulls in different seasons. Eight Murrah buffalo bulls maintained at Artificial Breeding Research Centre, ICAR-NDRI Karnal. Frozen semen samples of the bulls were obtained and sperm kinematic parameters were measured by computer-assisted sperm analyzer...
Article
Male fertility is an important factor in bovine reproduction as a single bull is generally used to breed numerous cows especially after the introduction of AI technology. Therefore prediction of fertilization potential of sperm becomes the primary goal of semen analysis. Visual evaluation of sperm motility in semen samples using light microscopy is...
Article
The present study compared the effect of mitochondria-targeted (Mitoquinone, MitoQ) and untargeted cytosolic antioxidant (Resveratrol, RESV) supplementation on lipid peroxidation (LPO) and in-vitro sperm functions of cryopreserved buffalo bull semen. To optimize additive’s concentration, sperm pellet obtained from twenty-four ejaculates was supplem...
Article
Full-text available
The objective of the present study was to measure pixel intensity mean (Echogenicity) and pixel intensity standard deviation (Heterogeneity) of testicular parenchyma in constantly low or high sperm concentration producing bulls. The average sperm concentration/ml in ejaculate over thirteen month's period (≥100 ejaculates) was recorded. On the basis...
Article
Full-text available
The objective of the present study was to measure pixel intensity mean (Echogenicity) and pixel intensity standard deviation (Heterogeneity) of testicular parenchyma in constantly low or high sperm concentration producing bulls. The average sperm concentration/ml in ejaculate over thirteen month's period (≥100 ejaculates) was recorded. On the basis...
Chapter
Full-text available
Simian immunodeficiency viruses (SIV) and human immunodeficiency viruses are belonging to the lentivirus subfamily of retroviruses with class VI, ssRNA-RT viruses (+ strand or sense) RNA with DNA intermediate in life-cycle. Genome has a special reverse transcriptase enzyme which serves to synthesize DNA from retrovirus RNA. India ranked 3rd in the...
Article
Livestock industry is one of the important pillars for country economy. Sperm sexing has the potential of significantly improving the quality and quantity of production in the livestock industries. Over years several attempts, involving several different parameters of sperm cells, have been made to develop an efficient sperm sorting procedure. Till...
Article
Full-text available
Background: The testicular biometry such as scrotal circumference (SC) and scrotal volume, affect semen quality hence bull fertility and thus testicular biometry can be used for breeding soundness of bulls. Methods: The present study was carried out to study the testicular parameters of 77 Sahiwal males of different ages and to establish its import...
Poster
Full-text available
Rabies: Preventable Fatal Zoonotic Disease, let's eradicate it by 2030
Article
Full-text available
Sperm, which are believed to be transcriptionally and translationally inactive, synthesize RNA and proteins before there is gradual disappearance of the ribosome during chromatin compaction. Sperm transfer several functionally relevant transcripts to the oocyte, controlling maternal-zygotic transition and embryonic development. The present study wa...
Article
Although reduced reproductive efficiency during summer has been well documented in buffaloes, the reason for the same is yet to be understood. The present study was conducted to identify the subtle differences in sperm phenotypic characteristics (motility, membrane integrity, acrosome reaction and lipid peroxidation status), oviduct binding ability...
Article
Full-text available
Semen contains epithelial cells that can be cultured in vitro. For somatic cell nuclear transfer applications, it is essential to know whether clone(s) produced from semen-derived epithelial cells (SedECs) are healthy and reproductively competent. In this study, the semen and fertility profile of a cloned bull (C1) that was produced from a SedEC we...
Article
The present study explored the effect of anandamide supplementation in the extender on quality of low sperm doses during cryopreservation in Sahiwal bulls. Each fresh semen sample was split into eight aliquots (I, II, III, IV, V, VI, VII and VIII). The aliquots I, II, III and IV were taken as control and diluted to 20, 15, 10 and 5 million spermato...
Article
Full-text available
The present study was conducted to evaluate the effect of supplemental manganese (Mn), chromium (Cr) and cobalt (Co) on semen qualities in Sahiwal bulls. Twelve Sahiwal bulls divided into three groups T1, T2 and T3 based on their similar semen volume and concentration and were fed with 0, 1.5 and 2.0 times higher Mn, Cr and Co over and above the ba...
Article
Greater than optimal diluting of semen for producing a large number of doses containing relatively small numbers of sperm can lead to compromised quality of sperm, post-thawing. In the present study the French mini-straw plug position was modified and the effect of re-positioning was evaluated on the quality of sperm after thawing subsequent to cry...
Article
Full-text available
The effect of dietary supplementation of Cu and Zn on semen quality parameters and certain bio-chemicals parameters were evaluated in Murrah bulls. Twelve mature Murrah bulls (4-6 years of age) were divided into three groups (n=4) T1, T2, and T3 based on semen volume and concentration and were fed as per ICAR standard (2013). However, the animals w...
Article
Full-text available
The onset of uterine infection during postpartum period compromises uterine health, fertility, and productivity of dairy cattle. Endometrial innate immunity plays a key role in eliminating uterine infection and keeping the uterus healthy. Hence, the present study has been designed with the hypothesis that altered endometrial immune response around...
Article
Karan Fries is an Indian composite breed of cattle with superior milk production with a greater disease resistance than many other cattle. Understanding of the basic sperm and semen characteristics is necessary to develop the artificial insemination and sperm sexing methods. This study was conducted to evaluate the morphological, rheological and bi...
Article
Full-text available
The objective of the study was to characterize the term Doka and to capture morphological changes in teat to find out the relationship between Doka and onset of estrus in Murrah buffaloes (Bubalus bubalis). The Murrah buffaloes maintained at of Livestock Research Centre (LRC), ICAR-NDRI, Karnal were classified into two groups based on the lactation...
Article
Full-text available
Zebu bulls are a shy breeder and they exhibit optimum libido in the presence of females with estrus phase. Continuous semen collection with the use of male dummy leads to lack of adequate sexual stimulation. Therefore, the present study was designed to test the effect of estrus-specific molecule(s) for effective sexual preparation of donor bulls. T...