Syed M. Shahid

Syed M. Shahid
Eastern Institute of Technology · School of Health and Sport Science

PhD
Interested in analyzing the risk assessment for the development of Type 2 Diabetes Mellitus at local & global levels.

About

76
Publications
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Introduction
I have PhD in Health Science (Medical Biochemistry), currently working as Postgraduate Lecturer in Health Science at Eastern Institute of Technology (EIT). I have published 52 research papers in the journals of international repute, edited 3 books and research manuals in above mentioned fields of study, supervised 5 PhDs under my supervision. In addition, I have also presented my research at international forums, about 65 including conferences, symposia, workshops, training courses.

Publications

Publications (76)
Article
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This study was designed to investigate effect of salicylic acid supplementation in gentamicin-induced nephrotoxicity. For this purpose, twenty four male albino rabbits were divided into 4 groups (n=6); control group, healthy untreated rabbits; gentamicin group, received only gentamicin (80mg/kg); gentamicin + salicylic acid group, received gentamic...
Article
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Objective: This study was conducted to find out the possible association of Vitamin D receptor, caudal-related homeobox 2 (VDR-Cdx2) polymorphism with cancer in the given study group. Methods: In this study, 151 subjects (84 cases and 67 controls) were recruited from two local tertiary care hospitals of Karachi, Pakistan, suffering from various...
Article
The epidemiology and prevalence of Type 2 Diabetes Mellitus (T2DM) in today’s youth and tomorrow’s adults, is currently in alarming stages and goes hand in hand with Childhood Obesity (CO). This association causes detrimental impacts on child health and around 68% global population today are affected by it. T2DM is initiated in children with obesit...
Article
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The immunity and immune system functions to fight against infections are significantly impacted by inappropriate food and nutrition. Long term malnutrition is universally considered as the leading cause of immune system deficiency. A substantial proportion of the global population does not meet the recommended daily intake of nutrients. The COVID-1...
Article
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When it comes to nutrition, nearly everyone has an opinion. In the past, nutrition was considered to be an individual’s responsibility, however, more recently governments have been expected (by some) to share that responsibility by helping to ensure that marketing is responsible, and that food chains offer healthy meal choices in addition to their...
Article
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Hypovitaminosis D has become a pandemic in the world populations and is believed to be related with the diabetes mellitus (DM). The aim of study was to assess the status of vitamin D along withother parameters in type 2 diabetic patients. Vitamin D (VD), calcium (Ca), phosphorous (Ph), random blood glucose (RBS) and HbA1c levels were assessedin 192...
Technical Report
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Prostate cancer is an important health burden to the healthcare system of any country. However, with the current prostate-specific antigen biomarker having low predictive value even for diagnostic purposes, the challenge is still open to tackle this chronic disease. There have been a number of studies which have indicated and encouraged a multi-dir...
Article
The present study was aimed to account an eco-friendly synthesis of iron (Fe), copper (Cu) and silver (Ag) nanoparticles (NPs) using green tea and black tea leaves extracts. Synthesized NPs were characterized using SEM, FTIR, EDX and UV/Vis spectroscopy techniques. Antibacterial activity of NPs was assessed against methicillin- and vancomycin-resis...
Article
Prostate cancer is an important health burden to the healthcare system of any country. However, with the current prostate-specific antigen biomarker having low predictive value even for diagnostic purposes, the challenge is still open to tackle this chronic disease. There have been a number of studies which have indicated and encouraged a multi-dir...
Article
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Purpose: To evaluate the effect of differential food intake on metabolic risk markers in Type 2 diabetic individuals with diverse FTO gene alleles. Materials and Methods: The study was conducted at Baqai Institute of Diabetology and Endocrinology (BIDE) Karachi, Pakistan between March 2011 and May 2013. The present study is based on a previously pu...
Article
β -carotene is one of carotenoid natural pigments, which are produced by plants and are accountable for the bright colors of various fruits and vegetables. These pigments have been widely studied for their ability to prevent chronic diseases and toxicities. This study was designed to evaluate the effects of β-carotene on angiotensin converting enzy...
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Vitamin D was previously considered essential mainly for the bone health. However its extra skeletal role diverted attention of scientists worldwide. Recent studies explored its importance in almost all vital functions of the human body. Its deficiency has become a pandemic across the globe. This is evident in many studies correlating vitamin D def...
Article
Antioxidant, antimicrobial activities of essential oil from plant Lavendula steochs were studies by kinetics and thermodynamic approach. Hydro-distillation was used for the extraction of oil from the flowers of Lavendula steochs.-Antioxidant activity was performed using DPPH method, in which the IC50 showed that essential oil has good antioxidant a...
Article
To determine the effect of genetic variants within the FTO gene (rs9939609) on obesity related traits and type 2 diabetes in South Asian population of Karachi, Pakistan. A case-control study was conducted at Baqai Institute of Diabetology and Endocrinology (BIDE), Baqai Medical University situated in Karachi. A total of 296 patients with known type...
Article
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Gene deletions in GSTM1 and GSTT1 may result in tempering the activation and detoxification of several carcinogens and thereby may increase the risk of cancer pre-disposition. This study aims to investigate the clinical impact of glutathione-S-transferase GSTM1 and GSTT1 polymorphisms on squamous cell carcinoma of cervix (SCCA).The GSTM1 and GSTT1...
Article
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Three new carbazole alkaloids, mukoenigatin (1), bikoeniquinonine (2) and murrayadinal (3), were isolated from the aerial parts of Murraya koenigii, along with mukeonine-B (4). Their molecular structures were determined on the basis of spectral analysis including UV, IR, MS, and 2D NMR spectroscopy. The antimicrobial activity of different fractions...
Article
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Objective: To determine the association of SNP in FTO gene, rs9939609, with Metabolic Syndrome (MS) in type 2 diabetic subjects at a tertiary care unit of Karachi, Pakistan. Methods: We genotyped FTO rs9939609 SNP in 296 patients with type 2 diabetes from the Out Patient Department (OPD) of Baqai Institute of Diabetology and Endocrinology (BIDE). M...
Article
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Two series of N-phthaloylglycine derivatives were synthesized under Schotten-Baumann conditions. The first series consists of N-phthaloylglycine amides (4a-h), and the second one consists of benzimidazole derivatives of N-phthaloylglycine (6a-d). All the synthesized analogues were evaluated for their in vitro antimicrobial activity by using disc di...
Article
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Vegetation plays a significant role in determining ground and surface water of an area. The present study was aimed to investigate the impacts of exotic Eucalyptus plantation on the ground and surface water in district Malakand (34.50° N, 71.75° E) Pakistan. Two villages (Kot and Totai) were selected randomly for data collection with a sample size...
Article
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Background Post-discharge mortality among children with severe illness in resource-limited settings is under-recognized and there are limited data. We evaluated post-discharge mortality in a recently reported cohort of children with severe malnutrition and pneumonia, and identified characteristics associated with an increased risk of death. Method...
Article
The identification of chromosomes for routine cytogenetic analysis is based on quality of metaphases and good banding pattern. Fresh slides of human lymphocytes have been shown to produce good bands for the identification of chromosomes morphology. G-bands by Trypsin using Giemsa (GTG) banding of aged slides is generally considered hard to get desi...
Article
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Introduction: This study aimed to evaluate the protective effects of curcumin on angiotensin-converting enzyme (ACE) gene expression, oxidative stress and anti-oxidant status in thioacetamide (TAA)-induced hepatotoxicity in rats. Materials and methods: Total 32 albino Wistar rats (male, 200-250 g) were divided into six groups (n=8). Group 1: unt...
Article
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Hypocalcaemia is common in severely-malnourished children and is often associated with fatal outcome. There is very limited information on the clinical predicting factors of hypocalcaemia in hospitalized severely-malnourished under-five children. Our objective was to evaluate the prevalence, clinical predicting factors, and outcome of hypocalcaemia...
Article
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Diabetes mellitus (DM) has been a growing epidemic worldwide and poses a major socio-economic challenge. The leading cause of DM death is nephropathy due to end-stage renal disease (ESRD). This study aims to identify the possible association of I/D variants of the ACE gene and M268T (rs699) of the AGT gene of renin-angiotensin-aldosterone system (R...
Article
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Background: Risks of death are high when children with pneumonia also have severe acute malnutrition (SAM) as a co-morbidity. However, there is limited published information on risk factors of death from pneumonia in SAM children. We evaluated clinically identifiable factors associated with death in under-five children who were hospitalized for th...
Article
Angiotensin converting enzyme (ACE) is a key player of Renin Angiotensin System (RAS), involved in conversion of active product, angiotensin-II. Alterations in RAS have been implicated in the pathophysiology of various diseases involving heart, kidney, lung and liver. This study is designed to investigate the association of ACE gene expression in i...
Article
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Clinical signs of pneumonia are often veiled in under-five diarrheal children presenting with hypokalemia, making clinical diagnosis of pneumonia very difficult in such population. However, there is no published report that describes the influences of hypokalemia on the clinical signs of pneumonia in diarrheal children. Our objective was to assess...
Article
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Aim: This study was designed to investigate the beneficial effects of green tea administration on angiotensin converting enzyme (ACE) gene expression in CCl 4 -induced liver cirrhosis in rats. Materials and methods: A total of 24 male Wistar rats were divided into 3 groups as: Group 1, normal untreated rats; Group 2, CCl 4 - induced cirrhotic rats;...
Article
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A single PCR protocol for methicillin-resistant S. aureus (MRSA) housekeeping genes, carbamate kinase, shikimate dehydrogenase, glycerol kinase, guanylate kinase, phosphate acetyltransferase, triosephosphate isomerases, and acetyl coenzyme-A acetyltransferase for multilocus sequence typing was developed. This PCR strategy provides a simple, rapid a...
Article
This study was designed to study the relationship between serum nitric oxide and sialic acid in patients of diabetic nephropathy. Total 210 diabetic patients including 115 males and 95 females, suffering from diabetes and nephropathy (DN) were selected followed by informed consent and approval from institutional ethical committee. Equal number of a...
Article
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In clinical microbiology, phenotypic characterization is laborious as well as time consuming strategy and remains less discriminative among high virulent to less virulent clinically important strains. Induction of molecular techniques, allow more accurate and quick way out for identification of Staphylococcus aureus along with its virulence capabil...
Article
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Background: There is lack of information in the medical literature on predictors of hypoxemia in severely malnourished children with pneumonia, although hypoxemia is common and is often associated with fatal outcome in this population. We explored the predictors of hypoxemia in under-five children who were hospitalized for the management of pneumo...
Article
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Ponceaue 4R interaction with protein, Nisin and BSA was concentration dependent and may be used for protein assay. As the dye binds with almost all the proteins and current methodology may be used for the estimation of proteins in various food systems. During the course of present work staining with ponceau 4R of resolved proteins on PAGE (poly acr...
Article
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Diabetic nephropathy (DN) is one of the major complications of type 2 diabetes mellitus (T2DM) characterized by frequent microalbuminuria, elevated arterial blood pressure, persistent decline in glomerular filtration rate and high risk of morbidity and mortality. It encompasses long-term duration of diabetes, which has an effect on the minute blood...
Article
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Diabetes mellitus (DM) is a chronic metabolic syndrome that can lead to serious vascular complications. Diabetic nephropathy (DN) has been established as the leading cause of deaths in diabetes due to ESRF. The association between ACE gene polymorphism and onset of DN has not been explored in Pakistani diabetic patients. This study investigates the...
Article
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Background In spite of a high occurrence of Hepatitis Delta in the province of Sindh in Pakistan, no genetic study of Hepatitis Delta virus (HDV) isolates from this region was carried out. The aim of this study is to analyze the genetic proximity within local HDV strains, and relationship with other clades of HDV, using phylogenetic analysis. Resu...
Article
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Liver diseases are among the major health problems worldwide. Despite their increasing frequency, high morbidity and mortality, medical management is supposed to be insufficient; no therapy has successfully prevented the progression of hepatic diseases. Green tea is one of the commonly used beverages and considered as powerful antioxidant. This stu...
Article
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Salivary factors have been studied for their effects on the process of dental caries in patients of diabetes mellitus type 2. In this study, protective role of salivary pH, salivary flow rate, and salivary calcium is assessed in the patients of diabetes mellitus type 2 with dental caries. The samples of saliva were collected from 400 patients of di...
Article
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The chewing habits of betel quid (paan), areca nut (chaliya), raw tobacco, gutka, niswar and manpuri are the most common addictions in Pakistan, India, Taiwan and China. To observe prevalence of oral squamous cell carcinoma (OSCC), a total of 170 patients of OSCC were selected from the general population for the study followed by informed consent....
Article
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Diabetes mellitus is a chronic metabolic disorder that can lead to serious cardiovascular, renal, neurologic and retinal complications. Diabetes clustered with hypertension and nephropathy has become the leading cause of end-stage renal disease globally. This study describes diabetes, hypertension and nephropathy with reference to glycemic control,...
Article
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A number of mutations in extracellular matrix protein 1 (ECM1) that is a glycoprotein and expressed in skin and other tissues are reported to cause a rare, autosomal recessive disorder called lipoid proteinosis (LP). The peculiar manifestation of LP is hoarseness of voice caused by laryngeal infiltration in infancy. Skin and mucous membrane changes...
Article
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This study was designed to evaluate the possible protective role of salivary factors like salivary flow rate and adequate level of calcium, phosphate, and fluoride in diabetes mellitus type 2 patients with dental caries. A total of 398 diabetes mellitus type 2 patients with dental caries and 395 age- and sex-matched non-diabetic subjects with denta...
Article
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Colorants either synthetic or natural are commonly added to a variety of food systems to make them attractive and acceptable for the consumers. Our previous publications on Carmoisine, Allura Red, Sunset yellow and the present study showed that food colorants such as Erythrosine, Amaranth, Tartrazine, Quinoline yellow, Brilliant Blue bind with the...
Article
This study was designed to investigate the role of serum nitric oxide and sialic acid in the development of diabetic nephropathy as disease marker. Total 210 diabetic patients (age and sex matched) were selected followed by informed consent and divided into four groups (70 each) as I: control; II: diabetic; III: diabetic hypertensive; IV: diabetic...
Article
Full-text available
The extracellular matrix protein 1 (ECM1) is a glycoprotein, expressed in skin and other tissues. Loss-of-function mutation in ECM1 causes a rare autosomal recessive disorder called lipoid proteinosis. Lipoid proteinosis is presented by varying degrees of skin scars, beaded papules along the eyelid margins, variable signs of hoarseness of voice and...
Article
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An observational and comparative study was conducted to compare the functional outcome between the patients treated with conventional antipsychotic agent haloperidol and atypical antipsychotic agent Clozapine (clozaril). A total of 32 patients were included in the study with established schizophrenia according to (DSM iv). The data was processed on...
Article
This study was designed to investigate the role of serum nitric oxide and sialic acid in the development of diabetic nephropathy as disease marker. Total 210 diabetic patients (age and sex matched) were selected followed by informed consent and divided into four groups (70 each) as I: control; II: diabetic; III: diabetic hypertensive; IV: diabetic...
Article
Full-text available
Diabetic nephropathy is the leading cause of death that affects more than 40% of diabetic patients. Its metabolic derangements are frequently accompanied with electrolyte imbalances. This study was aimed to evaluate the electrolyte homeostasis during the progression of diabetic nephropathy in various stages of developing nephropathy. Patients admit...
Article
Objective: Diabetic nephropathy is the leading cause of death in diabetics in Pakistan. Various environmental factors are involved in the pathophysiology of the complications of diabetes. Cigarette smoking is foremost among the environmental factors that contributes to the progression of diabetic nephropathy; however, sufficient data on the effect...
Article
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Calcium concentration plays an important role in the development of diabetic complications in tissues permeable to glucose like liver, blood vessels of retina, kidney and central and peripheral nervous systems. This study was designed to investigate the possible involvement of intracellular electrolytes in antihypertensive effects of diltiazem in d...
Article
The study was designed to assess the urinary bladder carcinoma with respect of patient's age, sex and stages and grades. A total of 100 cases of diagnosed (for last five years) patients (mean age 58.05±11.96 years) of urinary bladder carcinoma visiting various hospitals in private sector in Karachi were selected on the basis of their biopsies were...
Article
This study was carried out to evaluate the possible protective role of some serum factors like pH and adequate level of calcium, phosphate and fluoride in dental caries. A total of 100 subjects of either sex, aged 10-40 were selected. Decayed, Missed and Filled Teeth (DMFT) were used as indices for scoring the dental caries and were distributed int...
Article
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The metabolic derangements and disturbances and their consequences in diabetes mellitus are well known more or less in details too. However, knowledge on the diabetic disorders in membrane functions and transport mechanisms is limited which is an essential factor in progression of the disease. Serum electrolytes were measured by flame photometer (C...
Article
To investigate the disturbances of serum and red cell electrolytes in association with membrane Na(+)-K(+)- ATPase activity as well as the status of serum Urea, Creatinine and osmolality in normotensive diabetic and hypertensive diabetic patients. Thirty normotensive and thirty hypertensive patients (age and sex matched) were selected along with th...
Article
Diabetes mellitus and hypertension frequently coexist. A variety of aberrations have been reported in the metabolism of body chief electrolytes (such as Na<sup>+</sup>, K<sup>+</sup>, Ca<sup>++</sup> and Mg<sup>++</sup>) and their transport systems in intra and extracellular environment in various metabolic disorders. The study was aimed to examine...
Article
Knee effusion is a common occurrence in elderly patients following hip surgery. We prospectively studied 106 patients undergoing proximal femoral surgery. These patients were examined pre-operatively, on days 4 and 8, and six weeks post operatively for the presence of an ipsilateral knee effusion. 71 patients had undergone Total Hip Arthroplasty (T...
Article
If construction managers are to carry out their responsibilities in the areas of construction and its support services, they need ready access to the right information [Lock 92]. Timely access to the right data, properly processed for decision making, can provide a competitive edge. And keeping track of information flow on a construction job site i...
Article
A total of 213 patients underwent the Ross operation at our institution between January 1990 and January 1999. Outcome was assessed in rheumatic (RH) patients and compared with that in patients with other etiology (non-RH). After exclusion of 69 patients with a follow up of <18 months, the study group comprised 144 patients (119 RH, 25 non-RH). Pat...
Article
Aneurysm formation in the left ventricular outflow tract related to the proximal end of the pulmonary autograft after the Ross procedure was present in 2 patients. Both occurred late after operation and were associated with prolapse of a leaflet of the autograft and significant regurgitation. Both were repaired with no immediate complications. Ther...
Article
This is a two-dimensional and Doppler echocardiographic study of the hemodynamic changes in patients with heatstroke and heat exhaustion. It demonstrates that the hemodynamic changes in severe heat exposure reflect a hyperdynamic circulation with tachycardia and high cardiac output states. Relative hypovolemia was more pronounced in patients with h...