Syed Shahid MusviTal Tech university · Chemistry and Biotechnology
Syed Shahid Musvi
Phd
Studying Origin firing in Human Cells
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Introduction
Develop therapeutics against Fibrosis
Skills and Expertise
Publications
Publications (3)
Platelet-derived growth factor (PDGF)-Tyrosine kinase has become a popular pharmacological target for different disorders. Platelet-derived growth factor (PDGF)-Tyrosine kinase inhibitors have recently been recommended as cancer treatments; nevertheless, selectivity and efficacy remain notable hurdles. PDGFs are a family of four cysteine-loop-like...
Pituitary adenylate cyclase-activating polypeptide (PACAP), a neu-roendocrine hormone of the secretin peptide superfamily, is mainlyproduced in hypothalamus. PACAP shows diverse effects on CNS, per-ipheral organs, and some hematopoietic cells by VPAC1 and 2 receptorsignaling [1]. Megakaryocytes (MKs) express the stimulatory G protein(Gs)-coupled PA...
Megakaryocytes (MKs), the largest cells in the bone marrow, are generated from hematopoietic stem cells (HSCs) in a sequential process called megakaryocytopoiesis in which HSCs undergo MK-progenitor (MP) commitment and maturation to terminally differentiated MK. Megakaryocytopoiesis is controlled by a complex network of bone marrow niche factors. T...
Questions
Questions (8)
I have been trying to get the luminescence of my desired cell line which is transfected with luciferase gene and GFP via Varioskan Lux Plate reader. I get a GFP reading but when I use the D-luciferin (1mg/ml in DMSO), working 100ug/ml) on live cells luminescence value is the same as wells which are empty. My queries are:
1. Is it right to dissolve the D-luciferin in DMSO
2. Why there is luminescence in empty wells, is there any way to put it at zero in software or luminescence should is enough high and then subtract the base level lumnisence.
Please suggest the troubleshoot and protocol.
When we design primer at the end we get 4 pair of primers. On what basis we should choose which one.
During Bone Marrow isolation ,how to bring down the cells to single cell suspension . if there are some clumps and we grow them like that ,will it affect the Macrophage differentiation .
What is the difference between miR-126-3p.1 & miR-126-3p.2 . i am not getting it .
I identified a new protein I suspect that it is a kinase how we can prove?
In Downs Syndrome we have extra Chromosome present , how we can treat this syndrome .Any technique which can be used in this regard.
since in ovarian somatic cells, the Rhi is absent, so there may be some any other factor for single-stranded piRNA