Swaleha Zubair

• Doctor of Philosophy
• Aligarh Muslim University

Neurodegenerative disorders such as dementia and Alzheimer’s disease (AD) have adversely devastated the health and well-being of the older community. Given that early detection might help prevent or delay cognitive disorders associated with AD, developing an effective diagnostic technique is deemed appropriate to control the disease. Despite advanc...

Introduction Machine learning (ML) algorithms and statistical modeling offer a potential solution to offset the challenge of diagnosing early Alzheimer's disease (AD) by leveraging multiple data sources and combining information on neuropsychological, genetic, and biomarker indicators. Among others, statistical models are a promising tool to enhanc...

Amid extensive breast cancer research, valuable data and findings often remain scattered across published literature, databases and web resources, posing challenges for researchers and practitioners in curating specific datasets, genes and relevant information. Hence, we developed BRCAFem (BReast CAncer of Females), an integrated database for breas...

Research Highlights • Focused study on utilizing machine learning abilities with bioinformatics analysis to handle complex genomics data. • The exceptional performance of boosting classifiers was observed for binary classification on gene expression data. • A panel of common genes were screened from different countries that may serve as universa...

In spite of its high effectiveness in the treatment of both leishmaniasis as well as a range of fungal infections, the free form of the polyene antibiotic amphotericin B (AmB) does not entertain the status of the most preferred drug of choice in clinical settings. The high intrinsic toxicity of the principal drug could be considered the main impeda...

Tumorigenesis is a multifaceted process, where multiple physiological traits serving as cancer’s distinctive characteristics are acquired. “Hallmarks of cancer” is a set of cognitive abilities acquired by human cells that are pivotal to their tumor-forming potential. With limited or no protein-coding ability, non-coding RNAs (ncRNAs) interact with...

Several recent studies have contributed to our understanding of the need for host directed immunotherapeutic strategies against Mycobacterium tuberculosis (Mtb), either concomitantly with classical chemotherapy or also as standalone approach. Nevertheless, most of these studies were focused on enhancing chemotherapeutic potential of standard anti-t...

Purpose This study aimed to assess the potential of the Clinical Dementia Rating (CDR) Scale in the prognosis of dementia in elderly subjects. Design/methodology/approach Dementia staging severity is clinically an essential task, so the authors used machine learning (ML) on the magnetic resonance imaging (MRI) features to locate and study the impa...

Alzheimer's disease (AD) is one of the most frequent neurodegenerative disorders in the elderly subjects. Since early detection can prevent or delay cognitive decline in the older subjects, it is desirable to develop effectual protocols for the diagnosis of the disease. Most of the existing diagnostic tools fail to improvise timely disease prognosi...

Identification of Potential Marker Genes and Key Pathways in Breast Cancer using Bioinformatics Analysis Abstract Breast Cancer has been a prime concern worldwide due to the alarming increase in the number of diagnosed cases every year. The heterogeneous properties of breast cancer subtypes ignite the demand for strong prognostic markers to signif...

Identification of Potential Marker Genes and Key Pathways in Breast Cancer Using Bioinformatics Analysis

Breast Cancer is reckoned amongst the most common cause of morbidity and mortality among women, adversely affecting female population irrespective of age. The poor survival rate reported in invasive carcinoma cases demands the identification of early developmental stage key markers. MicroRNAs are contributing a critical role in gene regulation pote...

Biomarker Identification in Ductal Breast Carcinoma Through Gene Clustering and Classification Using Machine Learning Techniques Abstract Ductal breast carcinoma has continued to be one of the leading causes of morbidity and mortality among the female population across the world. Ductal carcinoma in situ becomes life threatening when it develops i...

With the advancement of high throughput sequencing technologies many complexities of cancer are gradually unwinding, thereby enabling identification of a wide range of breast cancer related genetic alterations but there are many complexities that remains to be elucidated which can further help to eradicate this life taking disease. The MUPP1 expres...

During the last 2 years, the entire world has been severely devastated by the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) pandemic (COVID-19) as it resulted in several million deaths across the globe. While the virus infects people indiscriminately, the casualty risk is higher mainly in old, and middle-aged COVID-19 patients. The i...

Tuberculosis (TB) is a significant and continuing problem worldwide, with a death toll of around 1.5 million human lives annually. BCG, the only vaccine against TB, offers a varied degree of protection among human subjects in different regions and races of the world. The majority of the population living near the tropics carries a varying degree of...

Background: Recent Coronavirus Disease 2019 (COVID-19) pandemic has inflicted the whole world critically. Although India has been listed amongst the top ten highly affected countries to date, one cannot rule out COVID-19 associated complications in the near future. Aim & Objective: We aim to build the COVID-19 severity model employing logistic func...

The recent advancement in the healthcare domain results in the generation of a large amount of clinical, imaging, and medication data. The extensive analysis of such data targets employing big data analytics helps in the timely identification of various diseases which thereby aids in building precautionary measures. Alzheimer's disease (AD) is the...

The recent advancement in healthcare domain results in the generation of a large amount of clinical, imaging, and medication data. The extensive analysis of such data targets employing big data analytics helps in timely identification of various diseases which thereby aids in building precautionary measures. Alzheimer's disease (AD) is the most com...

Neurodegenerative diseases such as Alzheimer’s disease (AD) and dementia are gradually becoming more prevalent chronic diseases, characterized by the decline in cognitive and behavioral symptoms. Machine learning (ML) is revolutionising almost all domains of our life, including the clinical system. The application of ML has the potential to enormou...

[This corrects the article DOI: 10.3389/fmicb.2018.02469.].

Timely uncovering of various dementia stages is vital in formulating effective treatment strategies for Alzheimer disease. The high-resolution MRI can be progressively exploited in the classification of various stages of dementia that in turn help in the development of efficient therapeutic stratagems. Considering the fact that analysis of the huge...

[This corrects the article DOI: 10.3389/fimmu.2020.00817.].

Dementia, a fatal and progressive neurodegenerative disease, poses a huge challenge to health care fraternity. A timely diagnosis of this intricate disorder can help in designing and formulating strategies helpful in effective regulation of the disease. There exists a huge amount of multivariate heterogeneous clinical data which can be developed by...

Visceral leishmaniasis (VL)-related mortality and morbidity imposes a great deal of health concern across the globe. The existing anti-leishmanial drug regimen generally fails to eliminate newly emerging resistant isolates of this dreadful parasite. In such circumstances, the development of a prophylactic strategy to impart protection against the d...

Background: Alzheimer disease (AD) is a degenerative progressive brain disorder where symptoms of dementia and cognitive impairment intensify over time. Numerous factors exist that may or may not be related to the lifestyle of a patient that result in a higher risk for AD. Diagnosing the disorder in its beginning period is important, and several te...

Objective: To evaluate and compare the performance of Random Forest (RF) ensemble classifier in imputation and non-imputation method of missing data values, and its impact to diagnose Alzheimer's disease (AD) based on longitudinal MRI data. Method: We studied 373 MRI sessions involving 150 AD subjects aged 60 to 90 years [Mean age ± SD = 77.01 ± 7....

Biomarker identification for progression of Ductal Carcinoma In-Situ.

Nano-sized drug delivery systems (NDDS) have been widely exploited to achieve targeted delivery of pharmaco-materials. Traditional pharmaceutical approaches, implied in the synthesis of nano-formulations, are obscure owing to the incompatible physico-chemical properties of the core drug as well as some other factors crucial in development of NDDS....

The Internet of Things (IoT) comprises enmeshing of communication via embedded devices using webwork networking devices. Cardio is considered to be the most prevalent ailment affecting people across the globe. We still fail to control it due to negligence and lack of adequate knowledge. A heart patient needs a regular checkup and monitoring but to...

Personalized medicine promises for quality treatment and better recovery of patients in near future. The success of personalized medicine can be attributed to the technology advancement that enable us to deal with big data. In spite of enormous amount of biological data and availability of wide range of technical support, the bottleneck remains the...

In the present study, we investigated potential of chitosan-based nanoparticles (CNPs) to deliver loaded therapeutic molecules to pathogen harboring macrophages. We fabricated stable CNPs employing ionic cross-linking method and evaluated their potential to target RAW 264.7 cells. The physicochemical characterization of as-synthesized CNPs was dete...

In the recent past, various groups have proposed diverse biocompatible methods for the synthesis of metal nanoparticles (NPs). Besides culture biomass, culture supernatants (CS) are increasingly being explored for the synthesis of NPs; however, with the ever‐increasing exploration of various CS in the biofabrication of NPs, it is equally important...

Mycobacterium tuberculosis (M.tb) contrives intracellular abode as a strategy to combat antibody onslaught. Additionally, to thrive against hostile ambiance inside host macrophages, the pathogen inhibits phago-lysosomal fusion. Finally, to further defy host cell offensives, M.tb opts for dormant phase, where it turns off or slows down most of its m...

Besides inciting persistent and recurrent nosocomial afflictions, Staphylococcus aureus (S. aureus), a biofilm forming pathogen, poses an increased risk of several skin as well as respiratory tract infections as well. Emerging antimicrobial resistance trend asks to search for an alternate non-antibiotic based option to combat S. aureus pathogen. In...

Protein aggregates have been reported to act as a reservoir that can release biologically active, native form of precursor protein. Keeping this fact into consideration, it is tempting to exploit protein aggregate-based antigen delivery system as a functional vaccine to expand desirable immunological response in the host. Herein, we explored the ca...

Biomimetic synthesis of nanoparticles offers a convenient and bio friendly approach to fabricate complex structures from simple components with sub-nanometer precision. In the present study, we have synthesized nanoparticles of the potent antifungal agent, Amphotericin B (AmB), using Aloe vera leaf extract. The synthesis of AmB nano-assemblies (AmB...

The present study discusses impact of BitcryptRansomware in the upcoming era of cashless economy. The wide usage of Bitcoins and e-transactions makes it mandatory to have knowledge about anticipated online threats which one can come across upon wielding of proposed cashless economy. Bitcoin can influence upon both legal as well as illegal dark web...

Background: For design of a subunit vaccine for tuberculosis, identification of antigenic T-cell epitope is of utmost importance. Several MHC prediction server are available that can accurately predict antigenic peptide of variable lengths. However, peptides predicted from one server not necessarily are predicted form another server, thus creating...

In the present study, we explored the antibacterial potential of ZnO-nanoparticles that were biomimetically synthesized employing conditioned culture supernatant of Staphylococcus aureus (S.aureus). The in situ synthesized ZnO-NPs were characterized employing various biophysical techniques including UV–vis spectroscopy, electron microscopy, DLS and...

Chronic hyperglycaemia in type 2 diabetes (T2D) is associated with increased oxidative stress and inflammation. Keeping the above fact into consideration we analyse the effect of age and gender on oxidative stress biomarkers and pro-inflammatory cytokines in T2D patients. The study included 148 diabetic and 110 healthy subjects, grouped on the basi...

Many diseases that were considered major affliction of mankind in the past have been successfully eradicated with introduction of appropriate vaccine strategies. In order to expedite new challenges coming up to deal with various infectious diseases, nano-particulate-based subunit vaccines seem to be the demand of ordeal. The nano-vaccines can find...

Commercially available amphotericin B (AmB) formulations are limited either by cytotoxicities, lower efficacies, shelf-life related issues or high production costs. AmB complexes based on poly(gamma-glutamic acid) (PGGA) have been prepared and evaluated for their efficacies against AmB-deoxycholate (Fungizone®) and liposomal AmB (AmBisome®). Physic...

Two series of novel flavones and flavone-tanaproget conjugates were designed, synthesized, 125I­radiolabeled, and evaluated for their antiproliferative activity against human cancer cell lines (MCF-7, MDA-MB-231, and HeLa). They exhibited good-to-excellent in vitro antiproliferative activity. For improving the receptor-binding affinity of synthesiz...

Background: Type 1 diabetes mellitus is a chronic inflammatory disease involving insulin producing β-cells destroyed by the conjoined action of auto reactive T-cells, inflammatory cytokines and monocytic cells. The aim of this study was to elucidate the status of pro-inflammatory cytokines and biochemical markers and possible correlation of these...

Contains clinical data of both healthy human subjects as well as data pertaining to various diabetic patients groups in the form of Tables. Table A contains clinical data of all the healthy subjects taken as controls, Table B contains clinical data of healthy control subjects in the age group ≤15 years, Table C contains clinical data of healthy con...

In general, the members of Lip gene family of Mycobacterium tuberculosis evoke strong immune response in the host. Keeping this fact into consideration, we investigated role of Rv3203, a cell wall associated protein with lipolytic activity, in imparting protection against experimental murine tuberculosis. The data of the present study suggested tha...

Proliferation of Rv3203 specific T lymphocytes isolated from immunized animals at two week post booster and also at 4 and 8 weeks post challenge upon stimulation with a fixed amount (50μg) of free Rv3203. Data represents the mean of three determinants ± S.D. (PB- post booster, PC-post challenge).Statistical significance was determined as described...

The sera of immunized animals were analysed for the evaluation of IgG2a: IgG1 isotypes by sandwich ELISA method. The data represent mean of three determinants ± S.D. and are representative of two different experiments with similar observation. (PB-post booster, PC-post challenge). Statistical significance was determined as described in materials an...

T cell proliferation response in various immunized groups to determine the effect of dose of Rv3203 on proliferation of T lymphocytes; splenocytes, isolated from various groups of immunized BALB/c mice at two weeks post booster time point, were co-cultured in the presence of increasing amounts (1.56 to 50 μg) of Rv3203 Ag in flat-bottomed 96 well p...

Contains Supplemetary Methods and Supplementary Results. Supplementary Methods contains following methods in detailed description (A) Culture, subcellular fractionation of M. tuberculosis H37Rv and western blot analysis, (B) Development and characterization of archaeosome based vaccine, (C) Preparation of Escheriosomes, (D) Assessment of antibody i...

Rheumatoid arthritis (RA) is a chronic, inflammatory autoimmune disease of unknown etiology. It is characterized by the presence of rheumatoid factor and anti-citrullinated peptide antibodies. Initial phase of RA involves the activation of both T and B cells. Cytokines have a crucial role in the pathophysiology of RA as pro-inflammatory cytokines s...

Various strategies adapted to develop an efficient vaccine against food-borne pathogen, Listeria monocytogenes have met with little success. Spheroplasts (bacterial cell devoid of cell wall), are likely to undergo membrane-membrane fusion, leading to the delivery of their content to the cytosol of antigen presenting cells, thus facilitating MHC cla...

Amyloids are highly organized protein aggregates that arise from inappropriately folded versions of proteins or polypeptides under both physiological as well as simulated ambiences. Once thought to be irreversible assemblies, amyloids have begun to expose their more dynamic and reversible attributes depending upon the intrinsic properties of precur...

RNAi based therapeutics holds great promises to be an effective strategy of the anti-gene realm in context of its therapeutic applications; however, despite significant potentials, its full efficacy cannot be realized in real sense owing to various confronts that plague its advancement. Efforts need to be driven on the development of safe, specific...

Nanotechnology has emerged as one of the leading fields of the science having tremendous application in diverse disciplines. As nanomaterials are increasingly becoming part of everyday consumer products, it is imperative to assess their impact on living organisms and on the environment. Physicochemical characteristics of nanoparticles and engineere...

Bi-functional antibodies with the ability to bind two unrelated epitopes have remarkable potential in diagnostic and bio-sensing applications. In the present study, bispecific antibodies that recognize human red blood cell (RBC) and the food borne pathogen Listeria monocytogenes (L. monocytogenes) were engineered. The procedure involves initial red...

The ease to culture, moderately less safety constraints in handling, and above all, hurdle free induction of an anticipated infection in mouse rendered Listeria monocytogenes the rank of a model organism for studying a variety of host immune responses. Listeria monocytogenes being an intracellular pathogen evokes potent CD8 T cell response during w...

Background Incidence of food-borne infections from Listeria monocytogenes, a parasite that has adapted intracellular residence to avoid antibody onslaught, has increased dramatically in the past few years. The apparent lack of an effective vaccine that is capable of evoking the desired cytotoxic T cell response to obliterate this intracellular path...

Biomimetic nano-assembly formation offers a convenient and bio friendly approach to fabricate complex structures from simple components with sub-nanometer precision. Recently, biomimetic (employing microorganism/plants) synthesis of metal and inorganic materials nano-particles has emerged as a simple and viable strategy. In the present study, we ha...

Graph represents differential cytotoxic effect of anticancer drug as well as antibody-conjugated gold nanoparticles against cancer cells as revealed by MTT assay. Plain gold particles were taken as control.

Surface Plasmon resonance of gold nanoparticles synthesized using Aloe vera leaf extract. Visible spectra of gold nanoparticles prepared by incubation of 5 ml of 10−3 M of HAuCl4 with increasing concentration of Aloe vera leaf extract for 24 h. The peak intensity of characteristic surface plasmon resonance (SPR) band of gold nanoparticles increases...

Background Nanomaterials are considered to be the pre-eminent component of the rapidly advancing field of nanotechnology. However, developments in the biologically inspired synthesis of nanoparticles are still in their infancy and consequently attracting the attention of material scientists throughout the world. Keeping in mind the fact that microo...

Cytokine response in splenocytes culture supernatant from various immunized groups at post booster on different time points. Archaeosome mediated Th1/Th2 polarization was ascertained by determining various cytokines at different time point post booster; (A) IFN-γ, (B) IL-12. To determine the antibody response IgG2a to IgG1 ratio was depicted using...

Western blot analysis of recombinant Rv3619c expressed in BL21 (λDE3) using pET-NH6 vector. The expressed recombinant Rv3619c was subjected to 15% SDS PAGE and transferred to nitrocellulose membrane. The membrane was blocked with 2% BSA in Tween PBS and finally incubated with polyclonal sera developed in mice. Lane 1: depicts protein marker, Lane 2...

The absence of certain genomic loci that are present in most of the virulent strains of Mycobacterium tuberculosis as well as lack of lasting memory responses are some of the major causes attributed to the non effectiveness of Bacille Calmette-Gue'rin (BCG) vaccine. Immunization schedules addressing these issues can offer better strategy for protec...

Biomimetic nano-assembly formation offers a convenient and bio friendly approach to fabricate complex structures from simple components with sub-nanometer precision. Recently, extract from cells of microorganism/plant origin have emerged as a simple and viable route for the synthesis of metal and inorganic material nano-particles. In the present st...

In the present study, we elucidated the effect of potassium salts on alkali denatured hen egg white lysozyme (EC 3.2.1.17) using intrinsic/extrinsic fluorescence as well circular dichroism (CD) spectroscopic methods. Intrinsic fluorescence studies revealed that various potassium salts mediate stabilization of lysozyme against alkali denaturation. F...