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January 2008 - October 2015
January 2008 - October 2015
January 2008 - October 2015
Publications
Publications (34)
It has been shown that the degradation of anionlc, cationic and ampholytic surfactants by pseudomonads can be controlled by the plasmids with size of 60-130 kb. Most plasmid strains that degrade surfactants are capable of conjugative transfer and elimination from bacteria cells. Restriction patterns of plasmids do not reveal significant homology be...
Spherical bismuth nanoparticles with average size 40 nm have been synthesized by the colloidal-chemical method in water medium. 100% of Bi content has been revealed in the nanoparticles by the method of energy-dispersive X-ray spectroscopy. Synthesized bismuth nanoparticles have been characterized as biosafe and biocompatible according to the Guide...
Unlabelled:
The aim of the work was the synthesis of gold nanoparticles (GNP) of different sizes and the estimation of their biological activity in vitro and in vivo.
Materials and methods:
Water dispersions of gold nanoparticles of different sizes have been synthesized by Davis method and characterized by laser-correlation spectroscopy and tran...
Aim. To study a possibility of usage of the alkaline gel electrophoresis method (Comet assay) for determination of genotoxic properties of veterinary vaccines preparations. Methods. The alkaline gel electrophoresis of isolated eukaryotic cells has been used with further visualization of the samples by fluorescent microscopy. Results. Veterinary vac...
Transconjugant strain of bacteria degraders of alkylnaphthalenesulphonate(ANS)--P Pseudomonas alcaligenes TR (NPL-41) was obtained under conditions of ANS selective pressure and flow cultivation of bacteria destructors of alkylbenzene sulphonate (ABS) P. alcaligenes TR (pABS) and naphthalene P. putida BS438 (NPL-41) and their immobilization on an i...
The present research is devoted to the investigation of a nanosystem including gold nanoparticles in an aqueous solution of sodium sulfacetamide in order to determine the nature and efficiency of their interaction depending on the concentration and pH of the medium. Sodium sulfacetamide is of interest due to the possibility of its use in the compos...
A portable voltammetric sensor for toxicity assessment was developed based on a screen-printed carbon electrode modified with SiO2-CuO-glucose oxidase (GOx) film. The method is based on the detection of the metabolic activity of E. coli towards glucose as an indicator system in the presence of antiseptic drugs as model toxic compounds. CuO particle...
Among the acquired mechanisms of resistance to antibiotics of microorganisms, the production of beta-lactamases, enzymes that inactivate penicillins, cephalosporins, carbapenems, and monobactams, is widespread. Most often, such beta-lactamases, in particular ESBL (extended-spectrum beta-lactamases), are capable of destroying III and IV generations...
Мета роботи. Обґрунтувати склад і технологію перспективних косметичних засобів з наночастинками срібла, золота і міді.
Матеріали і методи. Для розробки косметичних засобів використовували колоїдні розчини наночастинок срібла, золота і міді, синтезовані в Інституті біоколоїдної хімії імені Ф.Д.Овчаренка НАН України. Антимікробну активність колоїдни...
In dentistry and surgery a special role belongs to medications of the local action that possess the antimicrobial and anti-inflammatory properties and can stimulate the tissue regeneration.Aim. To study the antimicrobial activity of colloidal solutions of silver and gold nanoparticles in vitro, estimate their pharmacological activity in vivo and su...
Cadmium compounds are highly toxic substances characterized by mutagenic, genotoxic and carcinogenic effects, and having high cumulative properties. Application of cadmium nanoparticles (NPs) in medicine stimulates the study of their mechanism of action at the cellular level and at the level of organs and systems, determination of biomarkers of the...
Aim. The grounding of expediency of studying the nature of interaction between metal nanoparticles and plasmid DNA of bacteria at the stage of pharmaceutical development of antimicrobial preparations based on metal nanoparticles as separate antimicrobial agents, as well as compositions of metal nanoparticles with known antimicrobial substances.
Me...
Мета. Визначити перспективність і доцільність застосування наночастинок металів у біотехнології виготовлення вакцин, визначити стимулювальні концентрації наночастинок золота, срібла, міді, заліза для вакцинних штамів бактерій. Методи. Мікроскопічний, бактеріологічний, біологічний, хімічний, варіаційно- статистичний. Результати. Обґрунтовано доцільн...
In the last decade, ferromagnetic nanoparticles that are able to be heated under an AMF (alternating magnetic field) have gained considerable interest in the field of nanotechnology. The current study explores the peculiarity of the synthesis and the properties of Fe3O4 and (La, Sr)MnO3 nanoparticles by cryochemical and sol-gel technology, as well...
Water dispersion of 30 nm spherical silver nanoparticles (AgNP) has been synthesized and characterized as biosafe according to the parameters of cytotoxicity, genotoxicity, mutagenicity and biochemical markers. AgNP’ high bactericidal effectiveness in vitro has been revealed against wide spectra of pathogens (Escherichia coli, Staphylococcus aureus...
An increase of the number and the variety of anthropogenic sources of radio-frequency electromagnetic fields (RF-EMFs) causes a high interest in studying the effects of non-ionizing radio waves on living organisms. Amongst many biological targets, the DNA molecule has received the greatest attention with respect to potential RF-EMFs damage. The aim...
Septic complications are one of the most frequent complications and the leading cause of mortality in hospitals around the world. Its etiological structure is presented by a wide range of pathogens. Special significance today obtains the general spreading of microorganisms with multiple-drug resistance. These facts make topical the search of new, m...
This article presents results, which demonstrate the significant
efficacy of the studied silver nanoparticles solution (average
size 30 nm), synthesized at the Institute of Biocolloidal Chemistry
named after F. Ovcharenko NAS of Ukraine, as a potential
antimicrobial substance for use in oral and maxillofacial surgery
for treatment of acute purulent...
The expressed changes in the bacteria cells' plasma membrane ATPase activity values cells of Escherichia coli, which are used in the manufacturing of vaccines against cattle colienterotoxemia, have been revealed under the influence of lyophilization/rehydration process. Influence of gold (average size 20, 30, 45 nm) and silver (30 nm) nanoparticles...
The transconjugate containing hybrid plasmid (Te(R)Zn(R)lux+) was obtained using the conjugation method on Pseudomonasfragi T2(5) (Te(R)ZnR) strain and bioluminescent strain Protobacterium phosphoreum B7071 (lux+). The expression regulation of lux-genes on the obtained plasmid is carried out by promotor-operational area conjointly with zinc resista...
Effect of exogenous lipids on the morphology and enzymatic activity of Bacillus cereus B4368 membrane has been studied. Specific character of different lipids action on the membrane enzyme activity was found. This peculiarity had been taken into account by using liposomes in biological systems.
Transconjugant strain of bacteria degraders of alkylnaphthalenesulphonate (ANS) - P Pseudomonas alcaligenes TR (NPL-41) was obtained under conditions of ANS selective pressure and flow cultivation of bacteria destructors of alkylbenzene sulphonate (ABS) P.alcaligenes TR (pABS) and naphthalene P. putida BS438 (NPL-41) and their immobilization on an...
