Sutiman Sumitro

University of Brawijaya · Department of Biology

Mesenchymal stem cells (MSCs) have unique properties, including high proliferation rates, self-renewal, multilineage differentiation ability, wide multipotency, hypoimmunogenicity, noninduction of teratomas, and anticancer properties. MSCs can be isolated from embryonic and extraembryonic tissues as well as adult organs. Human Wharton's jelly stem...

Mesenchymal stem cells (MSCs) from Wharton's jelly have a higher proliferation rate and self-renewal capacity than adult tissue-derived MSCs. A low oxygen level or hypoxic condition is prevalent in the microenvironment of the stem cells in the early stages of development. Hypoxia can influence proliferation and differentiation of various stem/precu...

Background Both cortical and cancellous bone display a complex, porous microstructure whose properties depend on the macrostructure of bone as well as age and health of the individual. The aim of this study was to compare the microstructure and characteristics of mineral atoms in osteoporotic and normal bone. Methods A prospective laboratory expe...

This study aimed to evaluate the level of reactive oxygen species, oxidative stress, and NF-κB and p53 in undifferentiated nasopharyngeal carcinoma. Twenty-four nasopharyngeal carcinoma patients and ten normal subjects were involved in order to compare the level of malondialdehyde (MDA), hydrogen peroxide (H2O2), and peroxidative index (PI). The le...

Clinical research indicates that negative calcium balance is associated with low bone mass, rapid bone loss, and high fracture rates. However, some studies revealed that not only calcium is involved in bone strengthening as risk factor of fracture osteoporosis. Thus, in this report, the difference of metallic and nonmetallic elements in osteoporosi...

The purpose of this study is to ascertain how the problem-based learning approach affects students' critical thinking abilities in courses on conservation biology. This study design uses a non-equivalent control group in a quasi-experiment. Essay exam questions totaling 10 valid and reliable numbers served as the study's instrument. Pre-test and po...

H2O2 can provide a physiological response by activating Mitogen activated protein kinase (MAPK) signaling components, it can phosphorylate and activate several regulatory proteins, which are closely related to gene expression, and growth promotion. H2O2 has neuroprotective abilities and potential to be a complementary therapy. It can be implemented...

This research aims to determine the effect of PBL integrated with Igya Ser Hanjob's local wisdom on students' problem solving skills in the Conservation Biology course. The sample for this research was Biology Education Semester VII students taking Conservation Biology courses, totaling 48 students, namely class A (experimental class) with 24 stude...

Critical thinking and problem-solving are fundamental skills that students need to master. Various learning obstacles that occur during the pandemic of Covid-19 have hindered critical thinking and problem-solving skills training. This study aimed to enhance students' critical thinking and problem-solving skills through Complexity Science-Problem Ba...

The purpose of this study is to identify and analyze articles that examine students' critical thinking abilities that have been published in several reputable international journals published in period 2011-2020 using bibliometric analysis. The research was conducted using a systematic mapping method in 4 stages: (1) searching articles using the Pu...

The number of people with neurodegenerative disease continues to increase every year. A new perspective is needed to overcome this disease. In this review, researchers collected information about dysfunctional energy in neurodegenerative diseases driven by mitochondria. Mitochondrial dysregulation can cause damage to the neuron system. The increase...

Astaxanthin (ASX) is a natural biological antioxidant acceptable as a dietary supplement and food colorant. ASX has unique molecular characteristics which form stable formations in the presence of metal ions. This study aims to analyze the astaxanthin-Cu complex using FTIR, SEM-EDS, and XRD. The synthesis of the complex carried on ratios 1:1, 1:2,...

A progressive and chronic neurological condition called MSA (Multiple System Atrophy) is characterized clinically by varying degrees of dysautonomia, cerebellar ataxia, and parkinsonism. The concept of mitochondrial dysregulation in MSA is one perspective in handling this neurodegenerative disease. Damage to functional mitochondria is driven by the...

Incretin hormone is a new therapy for type 2 diabetes related to their activity for regulating blood glucose level. Even though, this hormone is destroyed by Dipeptidyl Peptidase-4 (DPP-4) become in-active form. DPP-4 inhibition will be beneficial for type 2 diabetes therapy. Empirically, some of local herbs are used by Indonesian people to cure di...

Alzheimer's disease is a chronic neurodegenerative illness that is one of the most persistent medical concerns with significant social and economic consequences. Current treatments, such as acetyl-cholinesterase inhibitors, only briefly alleviate cognitive decline and do not stop or reverse dementia progression. So the study aimed to illustrate the...

Mucoadhesive properties of herbs are the herb’s ability to strongly interact or penetrate in binding to mucin components in the mucus. The oral preparation of polyherbal made for therapeutic purposes must have a mucoadhesive capability. This in vitro study aims to determine herbal combinations that have a potential stable interaction with MUC2. The...

Critical thinking skills and concept mastery are essential for every individual to solve a problem. Critical thinking is an indispensable complex skill in the 21st century. This study describe that the contribution of critical thinking and concept mastery to problem-solving skills through CS-PBL is 65.3%. It could be concluded that CS-PBL is applic...

The number of people with neurodegenerative disease continues to increase every year. A new perspective is needed in overcoming this disease. In this review, researchers collected information about epigenetics and energy factors of neurodegenerative disease driven by mitochondria. Mitochondrial epigenetic dysregulation can cause damage to the neuro...

Natural antioxidants such as egg white are complex compounds that prevent cell damage from oxidative stress. Typically, the antioxidant confirmatory tests are carried out using a predetermined reaction time without kinetics. Therefore, this study was aimed at comparing the antioxidant activities of hen egg white (HEW), duck egg white (DEW), and qua...

Glycated human serum albumin (gHSA) undergoes conformational changes and unfolding events caused by free radicals. The glycation process results in a reduced ability of albumin to act as an endogenous scavenger and transporter protein in diabetes mellitus type 2 (T2DM) patients. Astaxanthin (ASX) in native form and complexed with metal ions (Cu2+ a...

The process of vitrification of the cumulus-oocyte complex (COCs) often results in cold shock. When warming, heat shock occurs which can disrupt the balance of intracellular calcium (Ca 2+) intensity. Drastic changes in temperature cause Reactive Oxygen Species (ROS), affecting changes on Ca 2+ in COCs. The role of calcium is needed for oocyte acti...

Jamu is well known as traditional herbal medicine that has been used by Indonesian for centuries ago. Local people usually consume jamu for maintaining health and therapy diseases because it is cheaper and has less side effects. Scientific approach needs to be done to study the characteristic of the herbals. This research aims to characterize Indon...

Implementing a proper learning model during the post COVID-19 pandemic is fundamental for learning quality enhancement, specifically for students' conceptual mastery. The research aims to develop a Complexity Science- Problem Based Learning (CS-PBL) model assisted by the Sistem Informasi Pengelola Pembelajaran (SIPEJAR) e-learning platform that is...

Cenderawasih Bay has extremely high biodiversity with an enormous amount of endemic species due to geography isolation. Tripneustes gratilla is one of the species that is abundant in the bay. The species has ecological value that is suitable for bio-indicators of the environmental condition. Since the Bay relatively isolated area, then we examined...

Tyramine-Fe complex is predicted present in Brotowali (Tinospora crispa) and play a role in its anti-inflammatory activity. Excessive inflammation in COVID-19 patients can be fatal and can even cause death. Anti-inflammatory drugs such as aspirin and ibuprofen inhibits the reaction of prostaglandin formation from COX 2 and arachidonic acid. This st...

Akway (Drimys piperita Hook f.) was known as an endogenous plant of Indonesia. This plant was investigated as free radical scavenger based on paramagnetic properties. This study aimed to identify the bioactive compound and the role of Fe metals ions to enhance the free radical scavenger capacity as Low molecular weight antioxidant (LWMA) of akway....

Ginger (Zingiber officinale) and meniran (Phyllanthus niruri L.) is a cultural heritage in Madura ethnic, Indonesia. Madurese herbs were traditionally boiled using pottery which is made from clay. Clay consists of Silica (SiO2), Aluminum silicate (Al2O3), Magnesium (MgO), Calcium (CaO),) Iron (Fe2O3) and Potassium (K2O). This component contributes...

21st century learning focuses on problem-solving skills. A higher level of education requires an understanding of a more systematic concept to elaborate problems. Problem-solving skill is one of the fundamental cognitive processes. It involves higher order thinking to discover solutions or ideas. This research aims to analyze the relationship betwe...

In this study, silver nanoparticles (AgNPs) were synthesized using aqueous extract of Piper retrofractum fruits by the sonication-assisted an eco-friendly method. The compounds of Piper retrofractum extract can perform as reducing as well as capping agent in the synthesis process of AgNPs. Silver nitrate was used as a starting material and was reac...

Determinant factors which contribute to the prediction should take into account multivariate analysis for capturing coarse-to-fine contextual information. From the preliminary descriptive analysis, it shows that environmental factor such as UV (ultraviolet) is one of the essential factors that should be considered to observe the COVID-19 epidemic d...

Coconut water (CW) is a nutritious drink containing highly complex components. Therefore, CW has an interesting unique physicochemical nature to investigate. This study aims to know the UV-Vis spectra of CW as flour solvent of soybean, black rice, and red rice. Each flour was homogenized with ratio of 1:10 (w/v) in fresh CW (f-CW) or heated-CW at 5...

This study aimed was to determine the optimal ratio, duration, and temperature reaction to form the astaxanthin-metal ions complex based on the UV-Vis spectrum profile and confirmation of complex formation using FTIR. The method used consists of the synthesis of the astaxanthin-metal ions (Cu2+ and Zn2+) and the determination of complex formation b...

Background: Mesenchymal stem cells (MSCs) are an appealing source of adult stem cells for cell therapy due to the high rate of proliferation, self-renewal capability, and applicable therapy. Wharton’s jelly (WJ), the main component of the umbilical cord extracellular matrix, comprises multipotent stem cells with a high proliferation rate and self-r...

Ethnopharmacological study of flavonoid compounds in Magnolia champaca (L.) Baill. ex Pierre as anti-inflammatory agents by molecular docking [Estudio etnofarmacológico de compuestos flavonoides en Magnolia champaca (L.) Baill. ex Pierre como agentes antiinflamatorios por acoplamiento molecular] Abstract Resumen Context: Magnolia champaca (L.) Bail...

Context: Magnolia champaca (L.) Baill. ex Pierre has traditionally been used by the culture to prevent and cure the inflammatory disease. Aims: To determine the benefit of M. champaca in the local community, especially in the treatment of tuberculosis, and investigate the potency of the flavonoid content of M. champaca as an anti-inflammatory agen...

Glycated human serum albumin (gHSA) undergoes conformational changes of proteins caused by free radicals. The glycation process results in a reduced ability of albumin as an endogenous scavenger in diabetes mellitus type 2 (T2DM) patients. Astaxanthin (ASX) has been shown to prevent gHSA from experiencing unfolding events and improve protein stabil...

Michelia champaca is used by the community to treat mild diseases in the population. The plant Michelia champaca produces anti-inflammatory com�pounds. Some anti-inflammatory classes of compounds include Phenols and Triterpenoids. In disease prevention and treatment mechanics, COVID 19 in particular, the ability of anti-inflammatory compounds is...

to see the complete video presentation, please click https://www.youtube.com/watch?v=gsajTE3sH44

The use of medicinal plants as the main alternative to solve respiratory disorders is widespread in the Ammatoa Kajang community. However, the Ammatoa Kajang’s knowledge, attitude, and practice of this medicinal plant use have not yet been revealed. This study was conducted to analyze the Ammatoa Kajang’s knowledge, attitude, and practice (KAP) of...

Determinant factors which contribute to the prediction should take into account multivariate analysis for capturing coarse-to-fine contextual information. From the preliminary descriptive analysis, it shows that environmental factor such as UV (ultraviolet) is one of the essential factors that should be considered to observe the COVID-19 epidemic d...

Ethnopharmacological study of flavonoid compounds in Magnolia champaca (L.) Baill. ex Pierre as anti-inflammatory agents by molecular docking [Estudio etnofarmacológico de compuestos flavonoides en Magnolia champaca (L.) Baill. ex Pierre como agentes antiinflamatorios por acoplamiento molecular] Abstract Resumen Context: Magnolia champaca (L.) Bail...

Balur is an integrative topical medication from Indonesia using herbal medicines: Moringa oleifera , Nicotiana tabacum , and Coffea arabica . Balur can be applied to treat various diseases including chronic diseases and autism because it performs to scavenge free radicals and release electron of heavy metals. However, the complexity of the balur me...

Jamu is a traditional medicine based on the wisdom of the local community can be used as an alternative to the treatment of several symptoms of the disease, one of which is pain, fever. The purpose of the research is to know the type and use of medicinal plants, the content of active compounds as well as prospective traditional herbal medicine for...

Traditional medicine using plants, animal materials, mineral substances, serian (generic) preparations, or mixtures of these ingredients that have been used from generation to generation for treatment based on experience and applied by the prevailing norms in society are called jamu. The research objective was to determine the types of medicinal pl...

This study aimed to identify potentially active compounds from mangosteen peel extracts that heal skin burns and to evaluate their molecular mechanisms. There are about 120 compounds that have been identified in mangosteen peel by datamining, including 60 types of xanthones. The SMILE format of each compound was downloaded from the PubChem database...

Tyramine-Fe complex is a bioinorganic complex that is formed in brotowali. The complex is thought to play a role in the anti-inflammatory activity of brotowali. The mechanism of anti-inflammatory drugs such as aspirin and ibuprofen is to inhibit the reaction of prostaglandin formation from COX2 and arachidonic acid. In this study, a docking simulat...

Azis S, Zubaidah S, Mahanal S, Batoro J, Sumitro SB. 2020. Local knowledge of traditional medicinal plant use and education system on their young Ammatoa Kajang tribe in South Sulawesi, Indonesia. Biodiversitas 21: 3989-4002. Kajang tribal community in Indonesia has been using plants as traditional medicine for long periods of time; however, this t...

The copper nanoparticles (CuNPs) have attracted much attention due to their application in diverse fields. The applications of CuNPs depend on their physical and chemical properties. This study presents the first report for the use of medicinal fruit extract of Piper retrofractum Vahl as an eco-friendly reagent in the synthesis of CuNPs using coppe...

Mangosteen has several important elements for biological activities such as anti-oxidant, anti-inflammatory, anti-bacterial and anti-cancer. There are γ-mangostin, α-mangostin, flavonoids, saponins, and tannins which could stimulate the collagen deposition and accelerate wound healing process. Burn injury cause deep wound in the skin and often caus...

This study aims to predict the degradation mechanism of cellulose substrate in silico from the results of isolation and test the potential of cellulolytic bacteria from rice fields in Greater Malang in producing cellulolytic enzymes, among others: β-1, 4 exoglunase, β-1, 4 endoglucase and β - glucosidase, identified isolates that have high potentia...

This research aims to computationally characterize HSA 4K2C protein and describe as well as its ability to bind transition metal ions. Data mining is performed to obtain HSA 4K2C from PDB and transition metal ions such as Cu2+ (ID: 27099), Fe2+ (ID: 27284), Mn2+ (ID: 27854), Mn3+ (ID: 105130) and Fe3+ (ID: 29936) from PubChem. The analysis consists...

Introduction: Breast cancer is one of the leading cause of cancer deaths in women. Metastasis in BC is caused by immunosurveillance deficiency, such NK cell maturation, low NK activity and decreasing cytotoxicity. This study was performed to improve activating receptors and cytotoxicity of NK cells using interleukins (ILs). Methods: Human recomb...

In this study, we want to characterize egg white protein-tomato extract complex based on the UV-Vis spectrum. The formulation of antioxidant complex Non-Freeze-drying (NFD) was made at a ratio of 1:10 (v/v) while the Freeze-drying (FD) sample was made at a ratio of 1:2 (v/v). The results showed that the absorption peak of FD complex profile value w...

UV (ultraviolet) light is an important factor should be considered to predict coronavirus epidemic growth pace. UV is different from weather temperature since UV is electromagnetic wavelength from 10 nm to 400 nm in size, shorter than of visible lights. For some people, UV light can lead to cancer from unprotected sun exposure, however, for tropica...

Background We investigated whether 10-gingerol is able to induce oxidative stress in cumulus cells. Methods For the in-vitro research, we used a cumulus cell culture in M199, containing 10-gingerol in various concentrations (0, 12, 16, and 20 µM), and detected oxidative stress through superoxide dismutase (SOD) activity and malondialdehyde (MDA) c...

Spodoptera litura is classified as a plant-disturbing organism. Efforts to control S. litura by using chemical insecticides have a detrimental impact on the environment and the potential to harm non-target organisms. Bioinsecticides provide a safe alternative for reducing the agricultural pest problem. The purpose of this study was to investigate t...

Breast cancer (BC) is the most prevalent type of cancer among women and one of the major causes of cancer mortality in women. Metastasis in breast cancer (BC) occurs due to immunosurveillance deficiency, including impairment of natural killer (NK) cell maturation. Conditioned medium (CM) from human Wharton’s jelly mesenchymal stem cells (hWJMSC-CM)...

Tuberculosis (TB) is one of the leading infectious diseases in the world. The disease is commonly caused by Mycobacterium tuberculosis (Mtb) bacteria which are capable of rapidly spreading through droplet transmission. In developing countries, poverty and malnutrition cause immunodeficiency which is considered as the main risk factor for the incide...

Objectives: Albumin in diabetes mellitus undergoes conformational changes that affect the ability as an endogenous scavenger. Treatment with astaxanthin (ASX) expected to improve the function of albumin in case of diabetes mellitus. The objectives of this study are to compare the capability of ASX and metformin to prevent conformational changes on...

This research aims to investigate the magnetic character of complex compounds from akway bark (Drimys piperita Hook f.) as a free radical scavenger. This research uses several analytical methods. Polarization microscopy used to characterize the birefringence of the crystals phenomenon. Inductively coupled plasma-Optical Emission Spectrometry (ICP-O...

This research aimed to prove the ability of extracted freeze-dried java plum fruit applied with a cigarette filter to decrease the intensity of free radicals in the cigarette smoke. It was reported that there was a difference in the spectrum infrared (IR) pattern, leading to a wavelength shift in the smoke burning from a cigarette after the applica...