Susie Boydston-White

• PhD Biochemistry, City University of New York
• Professor at Borough of Manhattan Community College

We classified breast cancer cell lines of different metastatic potentials using visible resonance Raman spectroscopy, principal component analysis, and support vector machines. Cross validated classification accuracies of over 78% were achieved.

Aim: The aim of the study is to test visible resonance Raman (VRR) spectroscopy for rapid skin cancer diagnosis, and evaluate its effectiveness as a new optical biopsy method to distinguish basal cell carcinoma (BCC) from normal skin tissues. Methods: The VRR spectroscopic technique was undertaken using 532 nm excitation. Normal and BCC human skin...

VRR spectroscopy was used for BCC and normal skin tissues with 532nm excitation. The spectra showed significant changes in collagen, carotenoids and lipids. These enhanced fingerprints demonstrate a potential use as label-free pathology method.

Resonance Raman spectroscopy is used for rapid detection of skin BCC cancer. The cross-validated classification accuracy is achieved to be as high as 98% using nonnegative matrix factorization along with support vector machine statistical method

A clear correlation has been observed between the resonance Raman (RR) spectra of plaques in the aortic tunica intimal wall of a human corpse and three states of plaque evolution: fibrolipid plaques, calcified and ossified plaques, and vulnerable atherosclerotic plaques (VPs). These three states of atherosclerotic plaque lesions demonstrated unique...

Resonance Raman (RR) spectroscopic technique has a high potential for label-free and in-situ detection of biomedical lesions in vivo. This study evaluates the ability of RR spectroscopy method as an optical histopathology tool to detect the atherosclerotic plaque states of abdominal aorta in vitro. This part demonstrates the RR spectral molecular f...

RR spectra of brain normal tissue, gliomas in low grade I and II, and malignant glioma tumors in grade III and IV were measured using a confocal micro Raman spectrometer. This report focus on the relative contents of tryptophan (W) in various grades of brain glioma tumors by the intrinsic molecular resonance Raman (RR) spectroscopy method using the...

Ceramic femoral heads have had promising results as a bearing surface in total hip arthroplasty. Our objective was to evaluate a series of retrieved alumina-zirconia composite ceramic femoral heads for evidence of the tetragonal to monoclinic zirconia phase transformation, metal transfer and articular surface roughness. Raman spectra showed evidenc...

Raman spectroscopy is a sensitive method to detect early changes of molecular _composition and structure that occur in lesions during carcinogenesis. The Raman spectra of normal, benign and cancerous breast tissues were investigated in vitro using a near-infrared (NIR) Raman system of 785 nm excitation and confocal micro resonance Raman system of 5...

Confocal resonance Raman (RR) spectra were collected from single proliferating cells and analyzed to detect spectral patterns that are cell-cycle dependent, as a consequence of cellular proliferation — normal or abnormal. The cells' biochemical age at each time point was confirmed by immunohistochemical staining to identify the presence or absence...

In this paper, the issue of the prion hypothesis, a simmering controversy within the scientific community, is addressed. We inquire into the appropriateness of the use of certain augmentations and rhetoric approaches used during scientific debates, as well as the aptness of unequivocal statements in textbooks that indicate "abnormal prions" as a pr...

In this paper, the issue of the prion hypothesis, a simmering controversy within the scientific community, is addressed. We inquire into the appropriateness of the use of certain augmentations and rhetoric approaches used during scientific debates, as well as the aptness of unequivocal statements in textbooks that indicate “abnormal prions” as a pr...

The resonance Raman (RR) spectra of six types of human brain tissues are examined using a confocal micro-Raman system with 532-nm excitation in vitro. Forty-three RR spectra from seven subjects are investigated. The spectral peaks from malignant meningioma, stage III (cancer), benign meningioma (benign), normal meningeal tissues (normal), glioblast...

Spectral cytology, the diagnosis of disease based on objective physical measurements on individual cells and subsequent computer‐based algorithmic interpretation, promises to provide faster and more reliable results than classical cytology. The measurements described in this review are based on well‐established vibrational microspectroscopic techni...

Introduction: definition and goals of spectral mappingExperimentalResults and discussionConclusions Acknowledgements

This comprehensive overview of biomedical applications of vibrational spectroscopy focuses on methodologies that are most relevant to biodiagnostics. After a few introductory chapters that summarize the current status of the field, the reference covers current spectroscopic applications; new spectroscopic directions; and study design and the analys...

We have previously reported spectral differences for cells at different stages of the eukaryotic cell division cycle. These differences are due to the drastic biochemical and morphological changes that occur as a consequence of cell proliferation. We correlate these changes in FTIR absorption and Raman spectra of individual cells with their biochem...

We report the first ever Raman and infrared microspectroscopic images of human cells at different stages of mitosis. These spectroscopic methods monitor the distribution of condensed nuclear chromatin, and other biochemical components, utilizing inherent protein and DNA spectral markers, and, therefore, do not require the use of any stains. In conj...

Spectral differences between normal and abnormal tissue observed to date appear to be due to different averaging processes of spectral patterns that differ according to the cell's biochemistry, due to its state of maturation, differentiation, and development. Thus, disease perturbs the distribution of cells in the different stages of maturation, di...

Instrumentation used in infrared microspectroscopy (IR-MSP) permits the acquisition of spectra from samples as small as 100 pg (10(-10) g), and as small as 1 pg for Raman microspectroscopy (RA-MSP). This, in turn, allows the acquisition of spectral data from objects as small as fractions of human cells, and of small regions of microtome tissue sect...

The ability of infrared (IR) spectroscopy to distinguish and map cancerous and non-cancerous tissue has opened the question of the origin of spectral differences between normal and cancerous cells. In this contribution, we report IR spectral maps of individual dried cancer cells, some of them in the process of cell division (mitosis), IR spectra of...

Human cells from different tissue types, and at different stages of disease, present slight differences in their infrared (IR) absorption spectra. Thus, IR spectroscopy offers the possibility of analyzing individual cells, or biopsy sections of tissues, for the presence of disease, based on totally objective measurements and computer-based analysis...

IR microspectral maps of healthy an diseased live tissue are reported, along with the methodology for obtaining such maps and methods for their interpretation. The result suggest that present technology permits maps to be collected that contain useful pathological information.

Advances in infrared spectroscopic methodology permit excellent infrared spectra to be collected from objects as small as single human cells. These advances have lead to an increased interest of the use of infrared spectroscopy as a medical diagnostic tool. Infrared spectra of myeloid leukemia (ML-1) cells are reported for cells derived from an asy...

An account is given on the state of the art in the interpretation of infrared microscopy of cells and tissues. Focus is on the subtle changes in the DNA/RNA spectral regions between normal, precancerous, and cancerous samples. This discussion aims at establishing the limits of infrared spectroscopy as a tool to diagnose cellular abnormalities that...

Infrared spectra of myeloid leukemia (ML-1) cells are reported for cells derived from an asynchronous, exponentially growing culture, as well as for cells that were fractionated according to their stage within the cell division cycle. The observed results suggest that the cells' DNA is detectable by infrared spectroscopy mainly when the cell is in...

This contribution is aimed at introducing a new spectroscopic endeavor, infrared spec-troscopy of entire cells and tissues, and demonstrating that this field has enormous potential as a diagnostic tool in medicine and cell biology.

"A dissertation submitted to the Graduate Faculty in Biochemistry ... " Thesis (Ph. D.) -- City University of New York, 1999. Includes bibliographical references (leaves 186-191).