Susana Casal

• PhD in Nutrition and Food Sciences
• Professor (Assistant) at University of Porto

Fermentable Oligosaccharides, Disaccharides, Monosaccharides, and Polyols, commonly referred to as FODMAPs, can aggravate symptoms of various gastrointestinal conditions, such as irritable bowel syndrome. Patients with these conditions often need to follow a low-FODMAP diet, requiring knowledge of FODMAP composition. FODMAPs are found in both natur...

The limited availability of pharmaceutical formulations tailored for cardiovascular diseases in both pediatric and geriatric populations generates the need for compounded dosage forms to guarantee precise dosing and medication adherence. This study aimed to analyze the physicochemical properties and stability of formulations of atenolol and enalapr...

Nowadays, consumers are more aware of the effects of their diet on their health, and thus demand natural or minimally processed food products. Therefore, research has focused on processes that assure safe products without jeopardizing their nutritional properties. In this context, this work aimed to evaluate the effects of high-pressure processing...

This book chapter explores protein carbonyl analysis in meat and meat products, focusing on the enduring power of the classic 2,4-dinitrophenylhydrazine (DNPH) spectrophotometric method. Understanding protein oxidation extent is crucial for product quality and safety. The chapter provides a thorough overview of the DNPH method, including its fundam...

Aiming to understand the nutritional impact of Moringa oleifera oil (MOO) on the quality of fried potatoes as consumed, a frying study using intermittent frying at 180 °C was conducted over 5 days, with a total heating time of 15 h, against olive (OO) and sunflower (SFO) oils. Additionally, due to MOO’s higher costs, a SFO/MOO blend (80/20 w/w) was...

Seeking to get insight into the close relationship between plant waxes and the climatic conditions of plants’ original biomes, the leaves of three Myrtaceae from the eastern Australian-Tasmanian region (Corymbia citriodora (Hook.) K.D.Hill & L.A.S.Johnson (lemon-scented gum), Eucalyptus gunnii Hook. (cider gum), and Eucalyptus globulus Labill. (blu...

In recent years, occurrences of heat waves and drought have become increasingly frequent, highlighting the undeniable impact of climate change. The rise in temperatures and decline in rainfall have had severe repercussions on olive trees’ behavior and olive oil production. This study aims to evaluate the effects of two-year climate variations on ol...

Poultry meat is an important part of the human diet, and the valorisation of autochthonous breeds is a determinant for the sustainability of the rural areas. The increasing demand for niche products demands for better knowledge of the carcass characteristics and meat quality of these local populations. The present study aims to characterise the roo...

Dairy cream, a common ingredient in various dishes and food products, is susceptible to rapid microbial growth due to its high water activity (≈0.97) and pH (≈6.7). Thus, it requires proper processing conditions to ensure food safety and extend shelf life. High-pressure processing (HPP) has emerged as a nonthermal food pasteurization method, offeri...

As an alternative to commercial whole egg thermal pasteurization (TP), the sequential combination of moderate pressure (MP) and/or ultrasound (US) pre-treatments prior to a shorter TP was evaluated. The use of US alone or in combination with MP or TP resulted in an inactivation that was far from that of commercial TP. Nevertheless, when these three...

Staphylococcus aureus is considered a high priority pathogen by the World Health Organization due to its high prevalence and the potential to form biofilms. Currently, the available treatments for S. aureus biofilm-associated infections do not target the extracellular polymeric substances (EPS) matrix. This matrix is a physical barrier to bacterici...

Deficit irrigation and kaolin foliar application are agronomic practices that have been demonstrated to improve productivity and physiological response in almond trees but there is no consistent information on the effects on the kernel composition. The objective of this study was to evaluate the effect of different Sustainable Deficit Irrigation (S...

Decabromodiphenyl-ether (BDE-209) is a persistent organic pollutant ubiquitously found in marine environments worldwide. Even though this emerging chemical contaminant is described as highly toxic, bioaccumulative and biomagnifiable, limited studies have addressed the ecotoxicological implications associated with its exposure in non-target marine o...

The impact and relative relevance of in vitro gastrointestinal digestion, processing temperature (room temperature or 180 °C), and gelling agent (GA) (carrageenan and alginate) on the bioactive compounds and oxidation status of olive and echium oils gelled formulations with 40% lipid incorporation was assessed. In vitro digestion was not affected b...

Olive oils from seven Portuguese regions were selected to study the effect of the geographical origin on the oils’ composition. Quality parameters, fatty acids, tocopherols, hydroxytyrosol and tyrosol derivatives, and oxidative stability were evaluated. All olive oils could be classified as extra virgin, and the geographical origin significantly af...

At olive oil industrial extraction, water addition is a practice overcoming the formation of thick pastes. The effect of water addition (0 to 6.2%, kg added water/kg olives), during the industrial milling of cv. Arbequina olives, on the oils’ chemical-sensory quality, was evaluated. Despite the extra virgin olive oil classification, compared with t...

Margarine, an emulsion constituted mainly of fat up to 90%, is used for highly variable cooking purposes. The susceptibility of lipids to oxidation needs processing operations/strategies that increase their shelf life and resistance to high temperatures such as the addition of synthetic antioxidants. Due to the toxicity of these later, the demand a...

Both vitamin A and E support female reproduction and embryonic development. These vitamins have been associated with decreased fertility or failure to end the pregnancy in animals. An observational study was conducted on follicular fluid (FF) samples to determine the concentrations of fat-soluble vitamins of women undergoing in vitro fertilization...

Leaves incorporation during the extraction of olive oils can enhance their chemical-sensory quality. Thus, leaves from cvs. Arbequina or Santulhana were added (1%, w/w) during the extraction of Arbequina oils using an Abencor system, being discussed the impacts on the phenolics and volatiles formation enzymatic pathways. Leaves addition contributed...

PC28 - Valorisation of endogenous wild fruits in Alto Minho region, Northern of Portugal: Centesimal composition and fatty acids profile of Myrtus communis L. PC29 - Characterization and Valorisation of endogenous wild berries in Northern Portugal: Centesimal composition and fatty acids profile of Tramazeira (Sorbus aucuparia L.) PC30 - Physicochem...

Chayote leaves are known for culinary and traditional medicine applications. This work intended to recover carotenoids and phenolic compounds from chayote leaves using the ultrasound-assisted extraction (UAE). A Box–Behnken design was employed to investigate the impact of extraction time, temperature, and ultrasonic power on the recovery of total c...

Arundo donax L. (Arundinoideae subfamily, Poaceae family) is a sub-tropical and temperate climate reed that grows in arid and semi-arid environmental conditions, from eastern China to the Mediterranean basin, suggesting potential adaptations at the epicuticular level. A thorough physical-chemical examination of the adaxial and abaxial surfaces of A...

The “Branca” breed is a dual-purpose Portuguese autochthonous chicken breed, produced in extensive systems and in small flocks, especially in the Entre Douro and Minho regions. A total of 40 birds (n = 20/sex) were slaughtered between 38 and 42 weeks (males) and 110 and 120 weeks (females), and carcass and meat quality parameters were evaluated. Th...

[This corrects the article DOI: 10.3389/fnut.2022.925771.].

The possibility of using a sublethal moderate pressure¹(MP) pre-treatment followed by a shorter and so gentler thermal pasteurization² (TP), (MP-TP), was assessed, as an alternative to commercial egg white³ (EW) TP, by evaluating the lethal efficacy against Salmonella Senftenberg and influence on the functional and physicochemical properties of EW....

Cv. Arbequina fruits were inoculated during one day with suspensions of Aureobasidium pullulans, Epicoccum nigrum or both species (10^4 spores/g olives), isolated from olive leaves, aiming to increase the phenolic content of the olive oils. Oils were extracted from non-inoculated (control) and inoculated olives, being evaluated for their oxidative...

Roasting is a key step for preparing sesame oil that leads to important changes in its or-ganoleptic properties and quality. In this study, white sesame seeds were roasted for 20 min in an electric oven at different temperatures (120, 150, 180, 210, 250 and 300 °C). The oils extracted from unroasted and roasted seeds were compared for their chemica...

Classically, vicariant phenomena have been essentially identified on the basis of biogeographical and ecological data. Here, we report unequivocal evidences that demonstrate that a physical–chemical characterization of the epicuticular waxes of the surface of plant leaves represents a very powerful strategy to get rich insight into vicariant events...

Classically, vicariant phenomena have been essentially identified on the basis of biogeographical and ecological data. Here, we report unequivocal evidences that demonstrate that a physical–chemical characterization of the epicuticular waxes of the surface of plant leaves represents a very powerful strategy to get rich insight into vicariant events...

Meat and fish are introduced into the diet as a source of protein, but these muscle foods present different fatty acid (FA) compositions and different lipid stabilities. Fatty fish is expected to oxidize due to its higher content of polyunsaturated FA (PUFA), whereas the higher heme-Fe content of red meat will also affect lipid stability. Combining...

Street food makes a significant contribution to the diet of many dwellers in low- and middle-income countries and its trade is a well-developed activity in the central Asian region. However, data on its purchase and nutritional value is still scarce. This study aimed to describe street food purchasing patterns in central Asia, according to time and...

The assessment of traditional products is important for the sustainability of agricultural systems and the preservation of a unique gastronomic heritage. The present study aims to determine the chemical composition of “Galo de “Barcelos”” (“Barcelos” Rooster) raw meat, used in the preparation of the typical Portuguese dish “Roasted Rooster from “Ba...

Raw milk (a highly perishable food) was preserved at variable room temperature (RT) under hyperbaric storage (HS) (50-100 MPa) for 60 days and compared with refrigeration (RF) under atmospheric pressure (AP) on quality, nutritional, and endogenous enzyme activity parameters. Overall, a comparable raw milk preservation outcome was observed between s...

Objective: To describe the availability and nutritional composition of commonly available street foods in Almaty, Kazakhstan. Methods: 384 street food vending sites (in 10 public markets) were assessed; information on vending sites’ characteristics and food availability and samples of the most commonly available street foods (81 homemade; 40 indust...

Two highly perishable dairy products (cow’s and goat’s fresh cheese (FC)), were stored under hyperbaric storage at room temperature (HS/RT, 50-100MPa) and compared with RF (4ºC), for 60 days. HS/RT (≥75MPa) lead to a more stable volatile organic and fatty acid profiles, reducing lipid oxidation rate, particularly for cow’s FC, resembling more to FC...

In this study, the potential of innovative (radiofrequency (RF) heating, high-pressure processing (HPP)) in combination with a renewable technology thermal solar energy (TSE)) to pasteurize fish soup was investigated. The performance of these technologies was compared to a conventional thermal treatment (CTT) using tubular heat exchangers. Thus, th...

High pressure pasteurised (HPP) milk was stored by hyperbaric storage at room temperature (HS/RT) (50-100 MPa at 20 °C) and compared with refrigeration/(RF), to assess the effect on two pathogens surrogates and a pathogenic, up to 120 days, and on fatty acids, volatile organic compounds (VOCs) and secondary lipid oxidation over 60 days. HS/RT (75-1...

This study aimed to define the shelf life of canned sardines after opening to increase consumer awareness of their quality and safety and reduce food waste. For this purpose, canned sardines (Sardina pilchardus) packed with different sauces were opened and stored at 4 °C for 7 days. Microbiological, sensorial, physical and chemical stability was mo...

Street foods can contribute largely for dietary sodium intake of populations in developing countries. We aimed to assess the variability in sodium and potassium composition of the most commonly available homemade street foods in Maputo city, capital of Mozambique. In a cross-sectional evaluation, researchers canvassed areas with 500-m diameter cent...

The indigenous fruit Strychnos madagascariensis is usually processed to flour, called nfuma, being highly consumed during staple food shortage. This study aimed to evaluate the nutritional composition of nfuma and its nutrient adequacy. Flours from four districts of Mozambique were analyzed using AOAC methods for proximate composition, HPLC for sug...

In Mozambique, rural communities produce flours from the dried pulp of Strychnos madagascariensis fruits. Owing to its high lipid content, the oil from this flour is frequently separated by pressing to be used as seasoning and medicine. Aiming to characterize this oil, flour samples (n = 24), dried at two different temperatures (55 °C and 65 °C), w...

The aim of this study was to produce different “table olive flours” from fruits at different maturation stages. “Table olive flour” is here presented as an innovative product that can gain importance as a bioactive rich ingredient. Three types of natural table olives from cv. Cobrançosa, i.e., green, turning color, and black olives, were soaked, fr...

Frost events and extreme weather phenomena greatly affect several characteristics of the olive fruit. This study evaluated the impact of frost on the morphology, composition (moisture, fat, fatty acids, tocopherols, and total phenolic contents), and antioxidant activity of olives of cv. Santulhana. A total of 14 trees from the same geographical reg...

The effect of olive leaves addition (1%, w/w, cvs. Arbequina or Santulhana), during the industrial extraction of Arbequina oils, on their physicochemical, color, phenolic profile, and sensory characteristics, was studied. Leaves’ incorporation reduced the primary oxidation (peroxide value by 33% and K232 by 17%) and increased the oxidative stabilit...

Oils from cv. Arbequina were industrially extracted together with olive leaves of cv. Arbequina or Santulhana (1%, w/w), and their olfactory and volatile profiles were compared to those extracted without leaves addition (control). The leaves incorporation resulted in green fruity oils with fresh herbs and cabbage olfactory notes, while control oils...

The olive oil composition of eleven cultivars (cvs. Arbequina, Arbosana, Arroniz, Cornicabra, Frantoio, Hojiblanca, Manzanilla, Picual, Picudo, Redondilla and Royuela) cultivated in a high-density olive grove located in a non-traditional producing region (Valladolid, Spain) was studied during four consecutive crop seasons. The results showed that t...

Valorização de bagas de plantas silvestres autóctones da região do Alto Minho: caraterização de Crataegus monogyna Jacq.

This study aimed to describe the street food purchased and its nutritional value in Chișinău, Moldova. A cross-sectional study was conducted in 68 street food vending sites. Data on the food items purchased were collected through direct observation. Nutritional composition was estimated through chemical analyses, food composition tables or nutritio...

This study aimed to describe street food purchases in cities from Central Asia, considering customers’ characteristics and the nutritional composition of the foods and beverages. Cross-sectional studies were conducted in 2016/2017 in Dushanbe (Tajikistan), Bishkek (Kyrgyzstan), Ashgabat (Turkmenistan) and Almaty (Kazakhstan). Direct observation was...

The aim was to describe the price of homemade street foods in four cities of Central Asia and Eastern Europe and to analyze its association with energy density, macronutrients, sodium and potassium contents. Cross-sectional evaluations of street food vending sites were conducted in Dushanbe, Tajikistan (n = 562), Bishkek, Kyrgyzstan (n = 384), Alma...

Robust and selective quantification methods are required to better analyze feed supplementation effectiveness with specific amino acids. In this work, a reversed-phase high-performance liquid chromatography method with fluorescence detection is proposed and validated for lysine quantification, one of the most limiting amino acids in ruminant nutrit...

The estimation of hydroxytyrosol-tyrosol derivatives amounts in olive oils through the analysis of olive pastes collected during malaxation is of utmost relevance due to the relationship with the oils’ health claim regarding the protection of blood lipids from oxidative stress. Olive pastes were collected during the industrial extraction of cv. Cob...

The integration of foodomics data to explain the impact of diet on health requires a precise knowledge of nutrients composition of complex meals. This work assesses the adequacy of two food composition databases (FCDBs) for calculation of nutritional composition of whole meals, compared to the golden standard “lab chemical analyses” and search for...

Hyperbaric Storage (HS) is being increasingly studied as a possible replacement or complement of refrigeration (RF). This work aimed evaluating the performance of HS to preserve raw pork in pieces (PP) and raw minced bovine meat (BM) at two different conditions (60 MPa/10 ºC and 75 MPa/25 ºC), by evaluating fatty acids, volatile organic compounds (...

The high proportions of monounsaturated fatty acids (MUFA) represent one of the most important technological and nutritional features of olive oils. The present study details the fatty acid (FA) composition of autochthonous cultivars (Lentisca, Madural, Redondal, Rebolã, Verdeal, and Verdeal Transmontana) produced from centenarian trees during five...

Phenolic compounds contribute to the bioactive properties of olive oil. However, olive oils can only support a health claim concerning the protection against oxidative stress depending on the polyphenolic concentration, requiring effective measures during extraction to preserve/enhance their concentrations. The effect of the malaxation temperature...

Olive oil is consumed worldwide due to its appreciated sensory attributes and health benefits, which are related with its fatty acid profile and richness in phenolic compounds that support a health claim concerning olive oil protective effects on blood lipids. However, to ensure that oils possess a rich phenolic content, the extraction conditions c...

Hyperbaric storage at room temperature (HS/RT: 75 MPa/25 °C) of vacuum-packaged fresh Atlantic salmon ( Salmo salar ) loins was studied for 30 days and compared to atmospheric pressure at refrigerated temperatures (AP/5 °C, 30 days) and RT (AP/25 °C, 5 days). Most of the fatty acids were not affected by storage conditions, with only a slight decrea...

Electronic tongues include any electrochemical non-specific multisensor device with global selectivity, aiming mimicking the human tongue taste capability. Electronic tongues have been widely and successfully applied as taste sensors in food and pharmaceutical areas showing huge potential as a complementary analytical tool for routine sensory analy...

Oleaster (Olea europaea var. sylvestris), or the wild olive tree, has great interest as a source of genetic material for olive breeding programs. Nevertheless, information about its oil composition is scarce. In the present work, the characterization of oleaster fruit morphology and oil chemical composition from three different tree populations in...

Background Urban areas in central Asia are currently undergoing nutrition transition. Street food is very popular, but the specific foods available and their nutritional composition are unknown. The aim was to describe the availability and macronutrient composition of street foods in Bishkek, Kyrgyzstan. Results Trained interviewers collected data...

High consumption of free sugars, particularly through soft drinks, represents a health concern on a global scale. Therefore, several measures, including taxation, were tentatively employed to reduce their intake. However, the decrease of sugars in soft drinks has been occurring mostly at the expense of adding non-nutritive sweeteners (NNS). This st...

The worldwide demand for sustainable protein sources is increasing. Moringa oleifera seed cake has a promising potential, as large quantities are available after oil extraction. Previous research showed that shells have a positive impact on the oil bioactivity, with this work aiming to understand the impact of shells’ presence in defatted flours fr...

Street food is popular in Eastern Europe, but its diversity and nutritional value is unknown. This study aimed to characterise the street food environment in Chișinău, Moldova, including the vending sites and vendors, food availability and nutritional composition of foods and beverages. All street food vending sites (single points of sale) located...

Tocopherols are compounds with high biological activity, beneficial for human health that can be found in vegetable oils like olive oil, contributing for its resistance to oxidation. In this work, the tocopherol contents of olive oils extracted from centenarian olive trees of six cultivars (cvs. Lentisca, Madural, Rebolã, Redondal, Verdeal, and Ver...

The impact of six culinary practices – oven/microwave combined with/without seasoning with oregano/beer – on lipid and protein oxidation of chicken burgers after cooking and after in vitro digestion was assessed. Five oxidation markers - malondialdehyde (MDA), 4-hydroxy-2-nonenal (HNE), hexanal (HEX), carbonyls, and Schiff bases – as well as free a...

Background: Environmental health is increasingly compromised by persistent toxic substances, which may have serious im�plications in food safety and, thus, in human health. Polybrominated diphenyl ethers (PBDEs) are anthropogenic contaminants with endocrine disruption abilities, which have been commonly found in food. Growing evidence points out th...

Edible flowers are a new gourmet product; however, they are not always available all years. Thus, it is essential to find out technologies to guarantee this product for a longer time. Flowers of four species (borage [Borago officinalis], heartsease [Viola tricolor], kalanchoe [Kalanchoe blossfeldiana], and dandelion [Taraxacum officinale]) were sub...

The reduction of the fish oil acidity is a significant problem in the rendering industry, as the oil’s range of applications and market value strongly depend on this parameter. In particular, the lower the acidity, the larger the oil’s market value. This work aims to study the potential of enzymatic esterification for reducing the fish oil acidity,...

Polybrominated diphenyl ethers (PBDEs) comprise a major class of brominated flame retardants and are well-known endocrine disruptors. The dietary route, through contaminated seafood consumption, is a main contributor to human exposure. Hence, the aim of this work was to provide thorough information on the dietary pathway of PBDEs and their methoxyl...

The present study aimed to evaluate the nutritional and bioactive potential of four edible flowers (borage, centaurea, camellia, and pansies). Significant differences were observed among the four. Water was the main constituent (>76%, fresh weight - fw). Linoleic and palmitic acids were the major fatty acids found in borage and red and yellow pansi...

Mediterranean olive heritage richness is poorly characterized. Olive oils from minor cultivars of Northeast-Portugal (Lentisca, Madural, Rebolã, Redondal, Verdeal and Verdeal Transmontana) from centenarian trees were chemical and sensory characterized, aiming to identify autochthonous cultivars capable of producing differentiated olive oils. All oi...

This study focused on the assessment of polybrominated diphenyl ethers (PBDEs) and their methoxylated metabolites (MeO-PBDEs) in estuarine seafood from the main Portuguese river, in order to evaluate their impact in the environment and the safety of consumers' health, thus providing a comparison with other world regions. For the purpose, PBDEs and...

Environmental health is increasingly compromised by persistent toxic substances, which may have serious implications in food safety and, thus, in human health. Polybrominated diphenyl ethers (PBDEs) are anthropogenic contaminants with endocrine disruption abilities and are commonly found in seafood, the main route of human exposure. Growing evidenc...

Background Street food (SF) is a strong tradition in Central Asia, where urbanization and westernization of food habits is occurring. Research on SF consumption is scarce, and crucial to understand its implications for public health. This study aims to describe the SF purchases in urban areas of Tajikistan, Kyrgyzstan, Turkmenistan and Kazakhstan....

The objective of this work was to study the effect of osmotic drying, using different hypertonic solutions (sucrose and sodium chloride), on physicochemical characteristics of pansies (Viola × wittrockiana). The same treatments were applied to lettuce to compare the behavior of flowers with other vegetables. Pansies' superhydrophobic surface struct...

Lipid stability, physical properties and volatiles profile of vacuum-packaged fresh Atlantic salmon (Salmo salar) loins were evaluated after hyperbaric storage at low temperature (HS/LT: 60 MPa/10 °C) and compared to atmospheric pressure and conventional refrigeration (AP/5 °C) after 5, 15 and 30 days, and at low temperature (AP/10 °C), after 5 and...

Street food is part of the culture in central Asia. Although nourishing foods can be found, unhealthier options are becoming increasingly popular – a marker of nutrition transition. The nutritional composition of street foods in urban settings is, however, unknown. The objective of this study was, thus, to characterise the street food availability...

Thermal processing remains the dominant method for food preservation, due to its low cost, high productivities and effectiveness on increasing products shelf-life. However, despite the fact that this treatment is necessary to destroy vegetative microorganisms that may be pathogenic or cause spoilage, and to inactivate enzymes, depending on the food...

Edible flowers have become the latest food fashion, such as in United Kingdom, Portugal and Australia, making this niche market very challenging. Producers and points of sale of edible flowers (supermarkets, local markets, online) are increasing around the world, whereas consumers and professional chefs are demanding higher quality products. Despit...

Purpose: L-Theanine is the major free amino acid present in tea (Camellia sinensis L.). The effects of several tea constituents on male reproduction have been investigated, but L-theanine has been overlooked. Sertoli cells (SCs) are essential for the physical and nutritional support of germ cells. In this study, we aimed to investigate the ability...