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A coding sequence (CD36-03230) from the yeast Candida dubliniensis had been previously annotated as a vanillin dehydrogenase (VDH). The corresponding protein (CD36-03230p) was recombinantly expressed in Escherichia coli and analysed. The protein is most likely a tetramer in solution as judged by crosslinking and gel filtration experiments. CD36-032...
Aldehyde dehydrogenases play crucial roles in the detoxification of exogenous and endogenous aldehydes by catalyzing their oxidation to carboxylic acid counterparts. This study reports characterization of two such isoenzymes from the yeast Saccharomyces cerevisiae var. boulardii (NCYC 3264), one mitochondrial (Ald4p) and one cytosolic (Ald6p). Both...
Background: Saccharomyces cerevisiae var. boulardii is the only yeast species with probiotic properties. It is considered to have therapeutic significance in gastro-intestinal disorders. In this paper, a comparative physiological study between this yeast and Saccharomyces cerevisiae (BY4742) was performed by evaluating two prominent traits of prob...
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is responsible for the current coronavirus disease 2019 (COVID-19) pandemic that has led to a global economic disruption and collapse. With several ongoing efforts to develop vaccines and treatments for COVID-19, understanding the molecular interaction between the coronavirus, host cells,...
Background & objectives: Pseudomonas aeruginosa is an important nosocomial pathogen since it can survive in minimal medium. There is a global emergence of multidrug resistant strains of Pseudomonas. These strains are the main causes of nosocomial infections causing morbidity and mortality as these infections are difficult to eradicate. The objectiv...
Carbon Nano Tubes could be either metallic or semi-conducting in nature, depending on their diameter. Its photocatalytic behavior has given an impetus to use it as an anti-microbial agent. More than 95% Escherichia coli and Staphylococcus aureus bacteria got killed when exposed to Carbon Nano Tubes for 30 minutes in presence of sunlight. Carbon Nan...
I have compared the metabolome of two strains along with some phenotypic and metabolic properties like antioxidant activities, etc. When reporting data I had performed t-tests and ANOVA wherever applicable within each experimental condition between each strain to elucidate the difference. But there is a suggestion of carrying out multivariate analysis to point out global difference between the two strains. Afraid that I don't really follow what I am expected to do here. Would appreciate any help please. Thank you
My enzyme kinetic assays yield a co-operative binding kinetics (allosteric sigmoidal) for the enzymes I am working on. As a result, I have the Khalf, apparent Vmax for each substrate. Now, I want to vary the co-factor and find out the true Km and Vmax. Could anyone suggest how to work out the concentration of the substrate that I need to keep constant while varying the cofactor concentration?
I would like to identify the genes/proteins responsible for production of a phenolic compound from this yeast. All possible random mutagenesis methods for phenotypic screening of mutants seem to work only if I know my genes of interest. Also the chances of genetic approaches to work look pretty grim because my yeast exists as a polyploid.