Sunil Kumar

• Ph.D.
• Professor (Assistant) at Higher Education Uttar Pradesh

Poxviruses belong to the family of double-stranded DNA viruses, and it is pathogenic for humans and spread worldwide. These viruses cause infections and various diseases in human. So, it is required to develop new drugs for the treatment of smallpox or other poxvirus infections. Very few potential compounds for the treatment of poxvirus such as sma...

Catharanthus roseus (L.) G.Don and Rauvolfia serpentina (L.) Benth. ex Kurz are well-known medicinal plants belonging to the family Apocynaceae that have been traditionally used as medicine since ancient times and distributed worldwide, especially in Asian and African continents. In the Ayurvedic system of medicine, the plant parts of both these pl...

The Grewia asiatica L. and Grewia serrulata DC. belongs family Tiliaceae have excellent medicinal and commercial importance and is distributed across the tropical and subtropical regions of the world. The preventative and curative properties of fruits from the genus are used to manage many routine health problems or diseases due to the presence of...

Vitex negundo L. is one of the aromatic shrubs belonging to the family Verbenaceae and widely distributed in America, Europe, Asia, and West Indies. In India, the leaves of V. negundo have been widely used as traditional Chinese medicine and Ayurvedic herbal medicine for the treatment of malaria, rheumatism, joint inflammation, menstrual cycle, fib...

Background : The genus Grewia (Tiliaceae) comprises about 321 species which are distributed in tropical and subtropical regions of the world. They have an excellent medicinal and emergent commercial importance and are traditionally used for the treatment of diabetes, typhoid, hepatitis, diarrhoea, dyspepsia, smallpox, syphilitic ulcers, eczema, mal...

Ethnopharmacological relevance The plants are from the genus Rauvolfia Plum. ex L. (Apocynaceae), which is represented by 74 species with many synonyms, and distributed worldwide, especially in the Asian, and African continents. Traditionally, some of them are used for the treatment of various disorders related to the central nervous system (CNS),...

Ethnopharmacological relevance: Catharanthus roseus (L.) G. Don is a well known medicinal plant belonging to family Apocynaceae that have been traditionally used as medicine since ancient times. C. roseus is a well recognized herbal medicine due to its anticancer bisindole alkaloids (vinblastine (111), vincristine (112) and vindesine (121)). In the...

Ethnopharmacological relevance: Catharanthus roseus (L.) G. Don is a well known medicinal plant belonging to family Apocynaceae that have been traditionally used as medicine since ancient times. C. roseus is a well-recognized herbal medicine due to its anticancer bisindole alkaloids (vinblastine (Serial Number (SN) 111), vincristine (SN 112) and v...

Ethnopharmacological relevance Andrographis paniculata (Burm.f.) Nees is a medicinal herb of the Asian countries used in many traditional medicinal systems for the treatment of diarrhea, flu, leprosy, leptospirosis, malaria, rabies, upper respiratory infections, sinusitis, syphilis, tuberculosis and HIV/AIDS etc. Aim of the study This review aims...

Picrorhiza kurroa Royle ex Benth. is a small medicinal herb of Alpine region of Himalaya placed under the family Scrophulariaceae. The plant is a creeping herb that becomes erect during the flowering period. Usually, the flowers are white or pale purple. The distribution range of this species in Southeast Asia and wide distribution is reported from...

Phyllanthus amarus Schum. and Thonn. (family Euphorbiaceae) is a small herb well known for its potent medicinal properties and widely used worldwide. P. amarus is an important plant reported in Indian Ayurvedic system of medicine to treat various problems related to the stomach, kidney, genitourinary system, liver, and spleen. It is bitter, astring...

Monoterpene indole alkaloids have gained significant interest due to their important medicinal properties and unique skeletal characteristics. There is an urgent need to develop simple, fast and suitable method for identification and characterization of monoterpene indole alkaloids in plant extracts. Therefore, ajmaline, ajmalicine and serpentine w...

Andrographis paniculata is traditionally used for the prevention and treatment of infectious diseases due to its bioactive terpenoid lactones and flavonoids. So, there is an urgent need to develop a liquid chromatography with tandem mass spectrometry method for identification and characterization of terpenoid lactones and flavonoids. The chromatogr...

Rapid and sensitive liquid chromatography with tandem mass spectrometry methods were developed for structural characterization and quantitative determination of bioactive compounds in Boerhaavia diffusa using high‐performance liquid chromatography with electrospray ionization quadrupole time‐of‐flight tandem mass spectrometry and ultra high perform...

Proanthocyanidins (PAs) are the most important and valuable polyphenolic compounds of Terminalia species (family Combretaceae) used for the treatment of several diseases. This study aims to seek identification and characterization of PAs in the ethanolic extracts of bark and fruit of six Terminalia species viz T. arjuna, T. bellerica, T. catappa, T...

Ficus and validation of the ultra performance liquid chromatography–electrospray ionization hybrid triple quadrupole–linear ion trap–tandem mass spectrometry (UPLC–ESI–QqQLIT–MS/MS) method in a multiple-reaction monitoring (MRM) mode for the quantitative determination of 19 phytochemicals. The chromatographic separation of targeted phytochemicals w...

A rapid, sensitive and reproducible method using ultra-high-performance liquid chromatography coupled with electrospray ionization hybrid triple quadrupole-linear ion trap mass spectrometry (UHPLC-ESI-QqQLIT-MS/MS) in multiple reaction monitoring (MRM) mode was developed and validated for simultaneous quantitation of anticancer (vincristine, vinbla...

The edible pseudo bulbs of Malaxis acuminata D. Don are used as a constituent of an Indian drug ‘Astavarga’ and in several marketed Ayurvedic formulations. The aim of the present work was to investigate the in vitro antiproliferative activity of the ethanolic extract and its fractions of the pseudo bulbs of Malaxis acuminata and to develop the HPLC...

Rationale: Catharanthus roseus is a well-known dicotyledonous medicinal plant containing diverse classes of bioactive terpene indole alkaloids (TIAs), in particular the anticancer agents, vinblastine and vincristine. In view of the commercial importance of these compounds there is an urgent need to develop an accurate and reliable method for the s...

Variations due to geographical location and dioecious nature have shown implications in the chemical and pharmacological properties of medicinal plants and their herbal products. Tinospora cordifolia is one of the most important dioecious plant distributed throughout India and very widely used in many herbal products and formulations. In this study...

Introduction: Tinospora cordifolia is a widely distributed medicinal plant used in various traditional and commercial Ayurvedic formulations. Due to the wide use of this plant it is important to know the extent of variability in the metabolite profile resulting from geographical location, season and gender. Objective: To develop a statistical ap...

Phyllanthus species plants are rich source of phenolics and widely used due to their medicinal properties. A liquid chromatography tandem mass spectrometry (LC-MS/MS) method was developed using high-pressure liquid chromatography coupled with quadrupole time-of-flight tandem mass spectrometry (HPLC-ESI-QTOF-MS/MS) for the identification and charact...

A rapid and versatile direct analysis in real time-time of flight-mass spectrometry (DART-TOF-MS) method was developed for chemical fingerprinting of P. amarus. Developed DART-TOF-MS method was applied to analyze nine samples collected from Uttar Pradesh (UP), West Bengal (WB) and Madhya Pradesh (MP). DART-TOF-MS fingerprinting data was analyzed us...

Thalictrum is a well recognised genus of medicinal plants. It is widely distributed in temperate and tropical habitats of India. Alkaloid isomers from Thalictrum are reported to exhibit various pharmacological activities like antiamebic, antimicrobial, antitumour and antiviral. Thalictrum reniforme Wallich and Thalictrum neurocarpum Royale are very...

Berberis species have been used extensively as raw material in various herbal medicines. In this work, a fast and sensitive method based on reversed-phase high-performance liquid chromatography coupled with electrospray ionization quadrupole time-of-flight tandem mass spectrometry (HPLC-ESI-QT OF-MS/MS) was used to assist the distribution and discr...

Mahonia leschenaultia (ML) and Mahonia napaulensis (MN) are lesser known and unexplored medicinal plants of the family Berberidaceae. They are used by the Todas of Nilgiris in their religious and medical practices but chemically less identified. Hence, it was decided to do extensive phytochemical analysis to explore the potential of these plant ext...

Adhatoda beddomei and Adhatoda vasica leaf, known as ‘Vasaka’ and/or ‘Vasa’ in Ayurveda and Malabar nut in English is an official drug in the Indian Pharmacopoeia. The medicinal properties of these plants are due to the presence of pyrroquinazoline alkaloids. An UHPLC–ESI/MS/MS method in both positive and negative electrospray ionization (ESI) in m...

Introduction: Rauvolfia serpentina is an endangered plant species due to its over-exploitation. It has highly commercial and economic importance due to the presence of bioactive monoterpene indole alkaloids (MIAs) such as ajmaline, yohimbine, ajmalicine, serpentine and reserpine. Objective: To develop a validated, rapid, sensitive and selective...

Rauwolfia species (Apocynaceae) are well known medicinal plants worldwide due to its potent bioactive monoterpene indole alkaloids (MIAs) such as reserpine, ajmalicine, ajmaline, serpentine and yohimbine. Reserpine, ajmalicine and ajmaline are powerful antihypertensive, tranquilizing agents used in hypertension and high blood pressure. Yohimbine is...

Coscinium fenestratum is extensively used as medicinal plant in traditional medicine to treat variety of ailments. Protoberberine and aporphine alkaloids are reported the major components in stem and root of this plant. In present study, quantification of eight bioactive alkaloids in different plant parts of C. fenestratum was done using ultra-perf...

Plants have been used since ancient times as an important source of biologically active substances. The aim of the present study was to investigate the phytochemical constituents (flavonoids and phenolics), antioxidant potential, cytotoxicity against HepG2 (human hepato carcinoma) cancer cell lines, and the antimicrobial activity of the methanol ex...

Terminalia arjuna is a medicinal plant used in ethnomedicine and the codified traditional medicine. A number of active constituents are reported, but there is no information on the whole range of gallic and ellagic acid derivatives present in this plant A rapid and sensitive analytical method was developed using reverse phase high-performance liqui...

Monoterpene indole alkaloids (MIAs) are medicinally important class of compounds abundant in the roots of Rauwolfia species (Apocynaceae). MIAs such as yohimbine (aphrodisiac agent) and reserpine (antihypertensive, tranquilizer) are the official drugs included in Model List of Essential Drugs of World Health Organization (WHO). Therefore, we have a...

Medicinal plants of the genus Rauwolfia (Apocynaceae) are extensively used as folk medicines worldwide. Its antihypertensive activity is well known due to the presence of monoterpene indole alkaloids (MIAs). The therapeutic potential of the herbal medicines are affected due to variation of bioactive phytoconstituents. Therefore, a rapid and validat...

Adhatoda vasica Nees is a well-known Ayurvedic medicinal plant, belonging to the family Acanthaceae. This study aims to seek identification and characterization of flavonoid C- and O-glycosides in the aqueous fraction of the plant leaves. A method was developed for simultaneous characterization of flavonoids and their glycosides using high-pressure...

Berberis species are well known and used extensively as medicinal plants in traditional medicine. They have many medicinal values attributable to the presence of alkaloids having different pharmacological activities. In this study, a method was developed and validated as per international conference on harmonization guidelines using ultra high perf...

RATIONALEAdhatoda beddomei and Adhatoda vasica are popular Ayurvedic medicinal plants in India, belonging to the family Acanthaceae. Tricyclic quinazoline alkaloids are found to be the most abundant in these plants which are responsible for broad-spectrum medicinal properties. This study aims to seek identification and characterization of those alk...

Thalictrum (Ranunculaceae) is a very widely distributed genus of medicinal plants in India and has been reported to have many therapeutic properties like diuretic, stomachic, antiseptic, aperient etc. Approximately 290 alkaloids were reported from the 80 species of the genus Thalictrum. Alkaloids from Thalictrum are reported to exhibit various phar...

Terminalia species are medicinal plants used in ethnomedicine and codified traditional medicine system. In this study, a rapid and sensitive analytical method was developed using reverse phase liquid chromatography coupled with quadrupole time of flight mass spectrometry (HPLC-ESI-QTOF-MS/MS) for qualitative analysis to find out array of flavonoids...