Sungchul . Shin

• LG Electronics, South Korea, Seoul

Wood has long been used in furniture, construction, and structural applications due to its durability, aesthetic appeal, and natural abundance. Beyond these conventional uses, recent attention has focused on the lightweight nature, sustainability, and distinctive structural features of wood, including aligned fibrous architecture and intrinsic poro...

Advances in biofabrication have enabled the generation of freeform perfusable networks mimicking vasculature. However, key challenges remain in the effective endothelialization of these complex, vascular-like networks, including cell uniformity, seeding efficiency, and the ability to pattern multiple cell types. To overcome these challenges, we pre...

While the human body has many different examples of perfusable structures with complex geometries, biofabrication methods to replicate this complexity are still lacking. Specifically, the fabrication of self‐supporting, branched networks with multiple channel diameters is particularly challenging. Herein, the Gelation of Uniform Interfacial Diffusa...

The biofabrication of three-dimensional (3D) tissues that recapitulate organ-specific architecture and function would benefit from temporal and spatial control of cell-cell interactions. Bioprinting, while potentially capable of achieving such control, is poorly suited to organoids with conserved cytoarchitectures that are susceptible to plastic de...

While the human body has many different examples of perfusable structures with complex geometries, biofabrication methods to replicate this complexity are still lacking. Specifically, the fabrication of self-supporting, branched networks with multiple channel diameters is particularly challenging. Here, we present the Gelation of Uniform Interfacia...

The biofabrication of three-dimensional (3D) tissues that recapitulate organ-specific architecture and function would benefit from temporal and spatial control of cell-cell interactions. Bioprinting, while potentially capable of achieving such control, is poorly suited to spheroids and organoids with conserved cytoarchitectures that are susceptible...

It is challenging to fabricate 3D architectures of conductive hydrogels and impart uniform conductivity at the same time. Here, we demonstrate a one-step 3D printing technique for controlling the 3D structure of hydrogel materials while simultaneously conferring uniform conductivity. The core technology lies in the in situ polymerization of conduct...

The generation of neural organoids from human pluripotent stem cells holds great promise in modeling disease and screenings drugs, but current approaches are difficult to scale due to undesired organoid fusion. Here, we develop a scalable neural organoid platform by screening biocompatible polymers that prevent fusion of organoids cultured in suspe...

Granular, microgel‐based materials have garnered interest as promising tissue engineering scaffolds due to their inherent porosity, which can promote cell infiltration. Adapting these materials for 3D bioprinting, while maintaining sufficient void space to enable cell migration, can be challenging, since the rheological properties that determine pr...

It describes a simple process to create soft spherical ferrogels by the biosynthesis of bacterial cellulose (BC). Gluconacetobacter xylinus forms the enclosed structure of the ferrogel, which is a hydrogel containing magnetic nanoparticles (MNPs). The core ferrogel is prepared by incorporating MNPs with cellulose nanofibers (CNFs) in culture medium...

Optimization of the rheological properties of the matrix is critical for high-fidelity matrix-assisted 3D printing (MAP), which enables the free-form fabrication of fluidic soft materials. This report describes the generic criteria observable in the printing process of cellulose nanofiber (CNF) hydrogels: the sharpness of an angled line, the cross-...

We investigate the three-dimensional (3D) structuring of cellulose nanofiber (CNF) foam-based ink using direct ink writing 3D printing and the transformation of CNF foam from an insulator to a conductor. The colloidal stability of a CNF foam is critical to producing a solid CNF foam which can be used as a template for the synthesis of conducting po...

Since shelf life of perishable foods is short, a compelling challenge is to prolong the freshness of foods with a cost-effective strategy. A perishable fruit, the strawberry, is chosen as a model perishable food and an edible film coating is applied to it using carboxymethylated cellulose nanofibers (CM-CNFs) stabilized by cationic salts. A transpa...

This work describes development of a silk fibroin (SF)-based ink material for digital light processing 3D printing. Riboflavin initiates the crosslinking of SF molecules under irradiation with visible light to form an SF hydrogel through dityrosine bonding. Rapid formation of a β-sheet structure induces shrinkage of SF hydrogels in phosphate-buffer...

A fused-deposition modeling (FDM) 3D-printed polyethylene terephthalate glycol (PETG)–sepiolite composite showed effective synergetic mechanical reinforcement in tensile testing compared to an injection-molded composite. The results showed that the addition of 3 phr sepiolite improved the tensile strength of 3D-printed PETG samples by 35.4%, while...

Colorimetric assay of melanogenesis inhibition was demonstrated with polyethylene glycol tetraacrylate (PEG-4A) hydrogel structures embedding melanocyte cells. The cell laden PEG-4A hydrogel structure was prepared by digital light processing printing using biocompatible visible range of light. The melanocyte in PEG-4A hydrogel structure showed a hi...

Cellulose nanocrystals (CNCs) are produced by sulfonic acid hydrolysis and used for the formation of Pickering emulsion (PE) with thyme white essential oil (EO). Highly volatile and hydrophobic thyme white is encapsulated in PE by the amphiphilicity of CNCs. Encapsulation of EO in a CNC shell is determined by confocal microscopy with distinct fluor...

Gluconacetobacter xylinus (G. xylinus) metabolism is activated by oxygen, which makes the formation of an air-medium interface critical. Here we report solid matrix-assisted 3D printing (SMAP) of an incubation medium surface and the 3D fabrication of bacterial cellulose (BC) hydrogels by in situ biosynthesis of G. xylinus. A printing matrix of poly...

An aqueous two-phase system (ATPS) is a water-in-water biphasic system, which is generally formed by two incompatible polymers. Recently, considerable effort has been dedicated to search for new ATPS polymer pairs in order to further expand ATPS’s applications. In this paper, a new ATPS system based on silk fibroin (SF) and alginate is introduced....

Carboxymethylated hydrophilic CNF (Hphil-CNF) was modified with methyltrimethoxysilane into hydrophobic CNF (Hphob-CNF) and used as a printing matrix. The Hphil-CNF hydrogel was printed at the surface of the Hphob-CNF hydrogel, forming an immiscible, distinct 3D structure. Fabrication of channel systems in the CNF platform was performed by matrix-a...

Silk fibroin (SF)-incorporated 4-arm polyethylene glycol acrylate (PEG4A) was used as a bioink for digital light processing three-dimensional printing. A visible range of light was adopted for the process to assure the safety of the cells in the bioink. Fibroblast cells were embedded in the printed hydrogels, and the keratinocyte cells were culture...

Polyvinyl alcohol (PVA) and cellulose triacetate (CTA) polymer solutions were electrospun to form nanofibers and microfibers, respectively. The bilayer of nano/micro fibers was prepared by electrospinning PVA nanofibers on the paper followed by electrospinning CTA microfibers on the top of a PVA nanofiber layer. The structure of electrospun fibers...

It is not easy to design structures with transparent solutions, especially in light projection 3-dimensional (3D) printing, since the penetration of light in solution is limitless. Here, silk fibroin incorporated with melanin nanoparticles (SFM) is used as a transparency modifier of poly(ethylene glycol)-tetraacrylate (PEG4A) solution. The incorpor...

To improve the cell suspension of the gelatin-methacrylate(GelMA) bioink, the viscosity of the ink solution was controlled by biocompatible silk fibroin(SF) particles. The viscosity of the GelMA solution increased about 1.4 × 10² folds by the addition of 1 w/v% SF particles. The increase of solution viscosity reduced the displacement of cells and r...

Cellulose nanofiber (CNF), one of the most attractive green bioresources, was adopted for construction of microfluidic devices using matrix-assisted 3D printing. CNF hydrogels can support structures printed using CAD design in a 3D hydrogel environment with the appropriate combination of rheological properties between the CNF hydrogel and ink mater...

Inadequate rheological properties of gelatin methacrylamide (GelMA) were successfully improved by incorporating cellulose nanofibers (CNFs), such that the printed scaffolds could maintain their structural fidelity during the three-dimensional (3D) bio-printing process. The CNFs provided an outstanding shear thinning property, and the GelMA/CNF inks...

We describe the nanocomposite membrane for cell encapsulation using nanocelluose hydrogels. One of the surfaces of bacterial cellulose (BC) pellicles was coated with collagen to enhance cell adhesion and the opposite side of the BC pellicles was coated with alginate to protect transplanted cells from immune rejection by the reduced pore size of the...

Here we describe a unique conductive bacterial cellulose (BC) composite with silicon nanoparticles (SiNPs) and polyaniline. BC was used as a template for binding SiNPs resulting in a very promising anode material for Li-ion rechargeable batteries that showed a high specific capacity. The surfaces of the SiNPs were modified with phytic acid to enhan...

The morphological variation of stimuli-responsive polypeptide molecules at the air–water interface as a function of temperature and compression was described. The surface pressure–area (π–A) isotherms of an elastin-like polypeptide (ELP) monolayer were obtained under variable external conditions, and Langmuir–Blodgett (LB) monolayers were deposited...

Natural cellulose hydrogel membrane cannot be directly used for cell encapsulation because it has many large pores on the surface that immune biomolecules are able to penetrate into easily. For the reason, alginate was used for the control of pore size of the cellulose hydrogel membrane. The surface morphology of cellulose/alginate nanocomposite co...

Nanofibrous 2,2,6,6-tetramethylpiperidine-1-oxyl(TEMPO)-oxidized bacterial cellulose (TOBC) was used as a dispersant of hydroxyapatite (HA) nanoparticles in aqueous solution. The surfaces of TOBC nanofibers were negatively charged after the reaction with the TEMPO/NaBr/NaClO system at pH 10 and room temperature. HA nanoparticles were simply adsorbe...

The objective of this study was to determine whether the genetic variants of CAPN1 developed in several cattle populations can be applied for Hanwoo, regarding genetic effects on meat traits. The traits were examined for 286 purebred Hanwoo steers with genotypes classified by restriction fragment length polymorphism (RFLP) and single strand conform...

Adiponectin (ADIPOQ) modulates several biological processes including energy homeostasis, glucose and lipid metabolism. The bovine ADIPOQ gene was located near the QTL affecting marbling, ribeye muscle area and fat thickness on BTA1. The gene encoding peroxisome proliferator-activated receptor-γ coactivator-1α (PPARGC1A) was located within the QTL...

Advanced dual control gate with surrounding floating gate (DC-SF) cell process and operation schemes are successfully developed for 3-D nand flash memories. To improve performance and reliability of DC-SF cell, new metal control gate last (MCGL) process is developed. The MCGL process can realize a low resistive tungsten (W) metal wordline, a low da...

A new Metal Control Gate Last process (MCGL process) has been successfully developed for the DC-SF (Dual Control gate with Surrounding Floating gate cell)[1] three-dimensional (3D) NAND flash memory. The MCGL process can realize a low resistive tungsten (W) metal word-line with high-k IPD, a low damage on tunnel oxide/IPD, and a preferable FG shape...

Quantitative trait loci for fat deposition and carcass traits have been identified in the vicinity of the gene encoding phosphodiesterase 1B (PDE1B) on bovine chromosome 5. Therefore, the PDE1B gene can be considered as a positional and functional candidate gene for carcass traits in beef cattle. This study aimed to identify single nucleotide polym...