Sulabha Sharma

• PhD
• PostDoc Position at Technical University of Darmstadt

Phototropins, the UVA‐blue light photoreceptors, endow plants to detect the direction of light and optimize photosynthesis by regulating positioning of chloroplasts and stomatal gas exchange. Little is known about their functions in other developmental responses. A tomato Non‐phototropic seedling1 (Nps1) mutant, bearing an Arg495His substitution in...

To meet the increasing demand for mass-produced microalgae, production processes must be optimised and monitored. The herein described optical sensors provide an instantaneous and direct opportunity to monitor biological and biochemical information for microalgae cultivation. Combination of expertise in natural science and engineering, as well as t...

Phototropins, the UVA-blue light photoreceptors, endow plants to detect the direction of light and optimize photosynthesis by regulating chloroplasts positioning and stomatal gas exchange. Little is known about their functions in other developmental responses. A tomato Non-phototropic seedling1 ( Nps1 ) mutant, bearing an Arg495His substitution in...

Tomato (Solanum lycopersicum) is a model crop plant for the study of fruit ripening and disease resistance. Here we present a systemic study on in planta transformation of tomato with Agrobacterium tumefaciens strain LBA4404 harboring pCAMBIA1303 binary vector bearing HPTII as a plant selectable marker and mGFP/GUS fusion as the reporter gene. We a...

Plants use gravity as a guide for growth and development. Gravitropism, a gravity-directed growth process, directs upward shoot movement for efficient photosynthesis and gaseous exchange. In addition, it also directs downward growth of roots in soil, for assimilation of water and nutrients required for growth and development. Using time lapse video...

Tomato fruit ripening is a fascinating process involving well-orchestrated coordination of several regulatory steps, which transform the green, unpalatable fruit to a fleshy aromatic red fruit, rich in carotenoids and other phytonutrients. Apart from regulation by a genetical hierarchy, a subset of fruit ripening is also regulated by light signalli...

In higher plants, blue light (BL) phototropism is primarily controlled by the phototropins, which are also involved in stomatal movements and chloroplast relocation. These photoresponses are mediated by two phototropins, phot1 and phot2. Phot1 mediates responses with higher sensitivity than phot2, and phot2 specifically mediates chloroplast avoidan...

Tomato fruit ripening is a complex metabolic process regulated by a genetical hierarchy. A subset of this process is also modulated by light-signaling, as mutants encoding negative regulators of phytochrome signal transduction, show higher accumulation of carotenoids. In tomato phytochromes are encoded by a multi-gene family, namely PhyA, PhyB1, Ph...

Higher plants use light as an information source for optimizing their growth and development. Among the photoreceptors sensing ambient light environment, phytochromes are most intensively characterized and mediate plethora of developmental responses ranging from seed germination, chloroplast development, flowering to senescence. Available evidences...

ABSTRACT In recent years, mutants have served as useful starting material to unravel the mechanisms governing many biological processes, including both plants and animals. Mainly, there are three ways of inducing mutations; by using physical agents (radiations UV, X-rays,y-rays), chemical agents (Ethyl Methane Sulphonate (EMS), Nitrous Oxide (NO),...

TILLING (Targeting Induced Local Lesions in Genomes) is a reverse genetics procedure for identifying point mutations in selected gene(s) amplified from a mutagenized population using high-throughput detection platforms such as slab gel electrophoresis, capillary electrophoresis or dHPLC. One essential pre-requisite for TILLING is genomic DNA isolat...

Background Rice is an important staple food and, with the smallest cereal genome, serves as a reference species for studies on the evolution of cereals and other grasses. Therefore, decoding its entire genome will be a prerequisite for applied and basic research on this species and all other cereals. Results We have determined and analyzed the com...

Background: Rice is an important staple food and, with the smallest cereal genome, serves as a reference species for studies on the evolution of cereals and other grasses. Therefore, decoding its entire genome will be a prerequisite for applied and basic research on this species and all other cereals. Results: We have determined and analyzed the co...

Rice is an excellent system for plant genomics as it represents a modest size genome of 430 Mb. It feeds more than half the population of the world. Draft sequences of the rice genome, derived by whole-genome shotgun approach at relatively low coverage (4-6 X), were published and the International Rice Genome Sequencing Project (IRGSP) declared hig...

The DNA sequence of 106 BAC/PAC clones in the minimum tiling path (MTP) of the long arm of rice chromosome 11, between map positions 57.3 and 116.2 cM, has been assembled to phase 2 or PLN level. This region has been sequenced to 10x redundancy by the Indian Initiative for Rice Genome Sequencing (IIRGS) and is now publicly available in GenBank. The...

An expression assay of randomly selected cDNAs resulted in the isolation of a novel cDNA (OSISAM) of SAM synthetase from rice. Nucleotide sequence data show that the cDNA is similar to a genomic clone (pRSAM1) from rice in the coding region but differs in the 5’ untranslated region (UTR) that is unique to this cDNA. The RNase protection experiments...