Suherni Susilowati

Airlangga University | UNAIR · Department of Veterinary Reproduction

Tanaman Obat Keluarga (TOGA) merupakan jenis tanaman yang memiliki dampak positif secara farmakologis pada tubuh manusia dan umumnya ditanam baik di lingkungan rumah tangga maupun dalam skala komunal. Penelitian ilmiah terhadap tanaman obat keluarga yang memiliki khasiat pengobatan juga mengungkapkan bahwa tanaman tersebut mengandung zat-zat atau s...

MSG can caused obesity that can affect metabolism in the body. The administration of EGCG can increase energy expenditure and metabolism. This study aimed to determine the effect of epigallocatechin 3-gallate (EGCG) on body weight and the percentage of abdominal fat of white rats (Rattus norvegicus) exposed to monosodium glutamate (MSG). Twenty-fiv...

One of the many efforts to increase the quality of livestock genetics is through artificial insemination (AI). Other than increasing it, AI can be conducted to preserve semen. A successful AI is determined by seminal quality, therefore, a method to preserve semen for a longer storage time is needed. The method used is adding an extender that fulfil...

Background and Aim The use of frozen goat semen for artificial insemination frequently results in a decline in sperm quality following thawing, which can be attributed to cold shock from cryopreservation, reduced motility, and possible DNA damage. Freezing may compromise mRNA stability due to the presence of free radicals. Despite strong post-thaw...

Cryopreservation is a technique utilized to store spermatozoa with the aim of preserving the quality of frozen semen. The quality of spermatozoa after thawing is a critical factor influencing the success of Artificial Insemination (AI). This decline in quality can be attributed to factors such as exposure to extreme temperatures, formation of ice c...

This study aims to determine the effect of the extract of Dayak onions (Eleutherine palmifolia) on the number of necrotic Sertoli and Leydig cells in mice (Mus musculus) induced with monosodium glutamate (MSG). This study involved 25 male mice aged 11 weeks and weighing approximately 20 g. The mice were divided into five groups, namely C- (0.5% CMC...

This study aims to determine the effect of the extract of Dayak onions (Eleutherine palmifolia) on the motility, viability, and concentration of spermatozoa in mice (Mus musculus) induced with monosodium glutamate (MSG). This study involved 25 male mice aged 11 weeks and weighing approximately 20 g. The mice were divided into five groups, namely C-...

This study aims to determine the species, prevalence, and infection degree of endoparasite in the digestive tract of laying hens in Suruhwadang Village, Kademangan District, Blitar Regency. Ninety-six fecal samples were taken from four different farms. Twelve samples of chicken feces aged 20-50 weeks and twelve samples of chicken feces > 50 weeks w...

Objective To improve the quality of post-thawing Boer buck semen for artificial insemination by adding green tea extract chitosan nanoparticles to skimmed egg yolk diluent, and the proper thawing temperature. Methods The ejaculate of Boer buck was added to skimmed egg yolk diluent without (the control group) and with adding 1 μg of chitosan nanopa...

The equilibration period is essential for sperm to adjust to the diluent, thereby preventing severe damage during cryopreservation. The elevation of lipid peroxidation during equilibration, indicating potential oxidative stress involvement, underscores the importance of optimizing equilibration protocols to minimize the detrimental effects of free...

Livestock production requires Sapudi rams, a breed native to Indonesia, to meet meat demand and food security. In artificial high-quality frozen semen is needed to spread Sapudi rams. To maximize the survival of spermatozoa during cryopreservation, semen should be stored in an extender. Green tea leaf extract (GTLE) and skim milk-goat egg yolk (SM-...

Livestock production requires Sapudi rams, a breed native to Indonesia, to meet meat demand and food security. In artificial high-quality frozen semen is needed to spread Sapudi rams. To maximize the survival of spermatozoa during cryopreservation, semen should be stored in an extender. Green tea leaf extract (GTLE) and skim milk-goat egg yolk (SM-...

The quality of frozen semen is one of the components of the artificial insemination program. The freezing method, kind of diluent, handling of frozen semen, and thawing process all affect the quality of frozen semen. This study aimed to determine the motility, viability, and plasma membrane integrity of frozen semen of Friesian Holstein cattle at s...

The purpose of this study was to determine the motility and viability of kampung rooster (Gallus gallus domesticus) spermatozoa in 0.9% Sodium chloride (NaCl) solution as an extender with the addition of egg yolk at different concentrations at cool temperature (5°C). This study was performed using two 1.5 years old healthy local roosters. Semen was...

The purposeof this study wasto determine the motility and viability of kampungrooster(Gallus gallus domesticus) spermatozoain 0.9% Sodium chloride (NaCl) solution as an extenderwith theaddition ofegg yolk at differentconcentrations at cooltemperature(5°C). This study was performedusing two 1.5 years old healthy local roosters. Semen was collected t...

The success of in vitro fertilization and embryo culture depends on the success of in vitro maturation. However, standard culture conditions usually increase reactive oxygen species (ROS), which have been implicated as a major cause for reduced embryonicdevelopment. It is well-known that higher than physiological levels of ROS trigger granulosa cel...

This study aims to determine the effect of adding melon (Cucumis melo L.) juice to Lactated Ringer’s-egg yolk extender on the intact plasma membrane (IPM) and morphological abnormalities of native rooster spermatozoa. Five ejaculate each from two native roosters (Gallus gallus domesticus) were divided into four equal volumes. Ejaculate was extended...

This study aims to determine the effect of adding melon (Cucumis melo L.) juice to Lactated Ringer’s-egg yolk extender on the intact plasma membrane (IPM) and morphological abnormalities of native rooster spermatozoa. Five ejaculate each from two native roosters (Gallus gallus domesticus) were divided into four equal volumes. Ejaculate was extended...

Background and Aim: The Kacang goat ( Capra hircus ) is an indigenous livestock species in Indonesia that is at risk of extinction due to cross-breeding. Artificial insemination (AI) techniques are expected to increase the population of these goats. This study aimed to determine the addition of epigallocatechin-3-gallate chitosan nanoparticles (EGC...

Pote goats are kept by the rural community in Bangkalan Regency, Madura Island, Indonesia. Estrus symptoms in goats are usually unclear, causing low fertility. This study aimed to determine the intracervical artificial insemination at early and mid-estrus on the Pote does. The 24 the does were randomly divided into two groups and estrous synchroniz...

Pote goats are kept by the rural community in Bangkalan Regency, Madura Island, Indonesia. Estrus symptoms in goats are usually unclear, causing low fertility. This study aimed to determine the intracervical artificial insemination at early and mid-estrus on the Pote does. The 24 the does were randomly divided into two groups and estrous synchroniz...

Background and Aim: The Kacang goat ( Capra hircus ) is an indigenous livestock species in Indonesia that is at risk of extinction due to cross-breeding. Artificial insemination (AI) techniques are expected to increase the population of these goats. This study aimed to determine the addition of epigallocatechin-3-gallate chitosan nanoparticles (EGC...

Preservation of ram semen has an impact on the exposure of artificial conditions to the spermatozoa which was followed by overproduction of reactive oxygen species (ROS) and resulted in functional damages of spermatozoa. This study aimed to determine the effect of the combination of chicken, quail, and duck egg yolks with green tea extract (GTE) on...

This study aimed to examine the effect of time differences on motility, viability, abnormality, and intact plasma membrane of spermatozoa of Sapera goats before freezing. This study used a Completely Randomized Design (CRD) with three settings, namely the equilibration time P1 (1 h), P2 (3 h), and P3 (4 h), each of six replications. The results of...

Objective: The objective of this study was to determine the effect of adding various doses of green tea extract to the semen of Kacang goats during the sexing process on motility, viability, membrane integrity, malondialdehide (MDA), and deoxyribonucleic acid (DNA) fragmentation. Materials and methods: It started with the containment of the seme...

This study aimed to determine the effect of the addition of cherry tomato, california papaya, and honey melon juice into skim milk extender in maintaining the quality of Sapudi ram semen at chilled temperature (5oC) storage. Five replication of ejaculates were divided equally into four groups. In control group (T0) semen was diluted in skim milk ex...

This study was the first to combine the addition of antioxidants to a skim milk–egg yolk (SM–EY) extender and different equilibration periods to obtain higher quality post-thawed Kacang buck semen. This study aimed to determine the effects of green tea extract (GTE) on the quality of frozen Kacang goat sperm equilibrated for one and two hours. The...

Background and Aim: 2,3,7,8-Tetrachlorodibenzo-p-dioxin (TCDD) is a persistent organic pollutant toxic to the human reproductive system. This study aimed to evaluate the effect of α-Tocopherol administration on the male fertility parameters of a rat model exposed to TCDD. Materials and Methods: Fifty healthy 12-week-old male rats were randomly div...

Background and aim: The production of male calf beef cattle is an agricultural innovation needed to increase the farm's productivity as a provider of meat sources. This study aimed to determine the sex ratio of the offspring of cows inseminated with Y-bearing sperm enriched by Percoll density gradient centrifugation and swim-up, combined with dela...

Kacang goats are small ruminants produced by low-income households in smallholder and farm to reduce poverty and prevent undernutrition. Studies to find a cryopreservation protocol for Kacang goat semen are expected to multiplication of genetically superior animals selected by the paternal lineage. This study evaluated the effect of thawing tempera...

The aim of this research was to know whether beetroot (Beta Vulgaris) extract could protect spermatogenesis by maintaining spermatogenic and sertoli cell count in rats (Rattus novergicus) induced with CCl4. Rats were given beetroot extract daily, for 14 days and 3ml/kg BW CCl4 intraperitoneally one hour after last treatment. This study used twenty...

Artificial insemination has proven to be an effective method for increasing population size and genetic quality of Kacang goats. However, innovation is required to maintain the quality of Kacang goat semen in storage. This study aimed to examine the effects of supplementing the 150 kDa protein assumed as IGF-I complex derived from bull seminal plas...

Background and Aim: Kacang buck sperm is cryosensitive due to the seminal plasma of semen itself. Meanwhile, bull seminal plasma contains the insulin-like growth factor-1 (IGF-1) complex, which is cryoprotective. The addition of the crude protein of Simmental bull seminal plasma increased the quality of post-thawed semen of Kacang buck. The study w...

Since the damage to alveolar tissue due to cigarette smoke exposure (CSE) is lipid peroxidation, antioxidant treatment is needed. The red guava (Psidium guajava L.) fruit contains antioxidants derived from quercetin, lycopene, and vitamin C. This study aimed to determine the effect of red guava fruit extract (RGFE) on the alveolar tissue of rats ex...

This study aims to determine the effect of red dragon (Hylocereus polyrhizus) fruit peel extract (RDFPE) on the parameters of Leydig cells number, seminiferous tubules diameter, and testicular weight of mice (Mus musculus) exposed to heat (40°C). Twenty adult male mice were divided randomly into five groups. The control group (C) mice only received...

High temperatures lead to oxidative stress, which can disturb spermatogenesis process. Watermelon (Citrullus lanatus) peel contain antioxidant expected to compensate oxidative stress due to heat stress exposure. This study aimed to determine the effect of watermelon rind ethanolic extract on the number of Leydig, Sertoli, and spermatogenic cells of...

The study aimed to determine the addition of green tea extract (GTE) in extender on the quality and DNA mutation of post-thawed Kacang buck sperm. The sperm DNA mutation was observed on nicotinamide adenine dinucleotide hydride (NADH) dehydrogenase 1 (ND1) of mitochondrial Deoxyribonucleic Acid (mtDNA). A pool of 12 Kacang buck ejaculates was dilut...

Spermatozoa in fresh semen of Sapudi ram has a limited life span. The storage of semen in cold temperatures (5 °C) is intended to prolong the spermatozoa's life. However, storage in cold temperatures can lead to increased production of reactive oxygen species (ROS). This condition reduces the quality of spermatozoa. The purpose of this study was to...

The genetic resources of Indonesia's indigenous Kacang goat require preservation. Artificial insemination is expected to accelerate population increases and preserve genetic resources simultaneously. The present study was the maiden attempt for cryopreservation of Kacang buck sperm. The objectives of this study were to determine whether the supplem...

The purpose of this study was to determine the reproductive efficiency of dairy cows in the presence of non-specific bacteria in the reproductive tract. This study was a descriptive study of 20 healthy and cycling dairy cows. The identification of non-specific bacteria in the reproductive tract was conducted in the previous study. Data were collect...

Repeat breeding is a reproductive disorder that harms dairy farmers because of the unachieved target to obtain one calf per year and decreased milk production. The repeat breeding syndrome can be caused by malnutrition. This study aimed to determine the effect of feeding silage, complete feed, and growth promoters in repeat breeder cows to restore...

The purpose of this study was to identify the correlation among the percentage of feed concentrate, milk urea nitrogen (MUN), milk production, services per conception (S/C), calving interval (CI), and days open (DO) of Holstein Friesian cows on smallholder farms. Twenty four, 4-6 year old, non-pregnant, lactating cows, with body condition score (BC...

Background: The effects of α-tocopherol on intracellular Ca2+ intensity in semen cryopreservation by regulate intracellular Ca2+ intensity have not been reported yet. Objective: The research was conducted to evaluate the effect of supplementation α- tocopherol into egg yolk skim milk extender on sperm quality and intracellular Ca2+ intensity. M...

Semen storage in cold temperatures might cause an increase in reactive oxygen species (ROS) production. This condition resulted in spermatozoa damage and quality decrease. This study was conducted to investigate the effects of α-tocopherol supplementation in diluents on the motility, viability, and plasma membrane integrity of Simmental bull sperma...

Objective: The aim of this study was to ascertain the effects of adding green tea extract to skim milk-egg yolk (SM-EY) extender on both the quality of post-thawed bull semen and the pregnancy rates of the recipient cows. Methods: Twelve ejaculates from four Simmental bulls, aged 3-5 years and weighing 900-950 kg, were diluted SM-EY extender, ad...

The purpose of this study was to determinated the effect and the best concentration of greentea extract in skim milk and egg yolk diluent for quality sapudi sheep spermatozoa were measured in motility, viability and intact plasma membrane that was stored on cold temperature. The semen was devided into four groups; skim milk and egg yolk diluent, 0,...

The purpose of this study was to prove that the addition of Vitamin B12 in egg yolk skim milk extender can maintain the motility and viability of post thawing Sapudi sheep spermatozoa. This study used sample of Sapudi sheep fresh semen that was collected with artificial vagina, and divided into 3 different treatments. The control treatment or P0, S...

This study aimed to determine the influence of mixture diluter of chicken egg yolk and young coconut water on the integrity of plasma membrane and abnormalities of Sapudi sheep spermatozoa. The experimental design used in this study was a complete random method. The data was analysed using ANOVA, and if there is a significant difference then procee...

The purpose of this study was to determine the effect and the best concentrasion of green tea extract in skim milk diluent for motility, viability, and intact plasma membrane Sapudi sheep spermatozoa that was stored 50C. The semen was divided into four groups: skim milk diluent; 0,05 mg green tea extract in skim milk diluent; 0,10 mg green tea in s...

The purpose of this research was determine the effect and the best concentration of greentea extract in egg yolk citrate diluent for quality Sapudi sheep spermatozoa were measured in motility, viability and intact plasma membrane that was stored on cold temperature. The semen was devided into four groups : egg yolk citrate diluent, 0,05 % green tea...

The purpose of study was to find out the effect of different concentrations of soya bean extractin sperm diluter, stored at 5°C toward sperm viability and necrosis. The semen was divided into four groups : P0: 0.1 ml sapudi ram + 1 ml of diluent yolk citrate, P1: sapudi ram 0.1 ml + 1 ml diluent soybeans extract konsentration 5%, P2: 0.1 ml sapudi...

The purpose of this study was to know the effect of L-arginine on the histology of primary spermatocyte count in mice (Mus musculus) after high temperature exposed. The subjects of this study were 20 adult male mice, 8 weeks old with an body weight range from 20-40 grams. This research conducted by using Complete Randomized Design (RAL) with 4 trea...

This research was aimed to determine the addition of L-arginine on motility and sperm plasma membrane of Sapudi sheep after two hour equilibration time in egg yolk tris diluent. This research used fresh samples of sheep Sapudi semen collected by artificial vagina, then were devided into 4 treatments and 6 replications. Analysis of the data using An...

Quality of spermatozoa motility and viability from rejected limousin bull semen diluted with skim milk egg yolk sitrat added with various levels of glucose. Glucose level used were 0%, 0,5%, 1,5%, 2,5%, and 3,5%. Writer was using on four years old Limousine bull. Bull semen used in this research was bull rejected semen with bellow 70% motility. Sem...

High temperatures lead to oxidative stress which can decrease the quality of spermatozoa. Watermelon’s rind have an ability for antioxidants effect that can reduce sperm’s damages that results by role explanation of free radicals. This study aimed to determine the effect of extract of watermelon’s rind on the motility and viability of sperm on male...

The aim of this study was to find out protein tyrosine kinase profile in Merino Sheep seminal plasma. This study consist of collecting semen of Merino Sheep containing plasma and spermatozoa, the separation between seminal plasma and spermatozoa, then tyrosine kinase analysis using Sodium Dodecyl Sulphate Polyacrilamide Gel Electrophoresis (SDS-PAG...

Aim: The aim of this study was to know crude guava leaf tannins effect on motility, viability, and intact plasma membrane of stored spermatozoa of Etawa crossbred goats. Materials and methods: Macroscopic assessment of normal Etawa crossbred semen was followed by dilution with a glucose solution at a 1:10 ratio to increase volume. The diluted se...

A study on the infestation of ectoparasites in chicken’s raised in Village of Kramat, District of Bangkalan, Regency of Bangkalan was conducted between July to October 2017. A total of 60 chickens were examined based on their sex and region of the chicken’s body. Identification was carried out on ectoparasites found identified using permanent mount...

Aim: The purpose of this study was to determine the benefits of L-arginine addition in skim milk extender to maintain the quality of goat spermatozoa in chilled storage. Materials and Methods: A total of 18 ejaculates from three healthy goats with weight and age of 45 kg and 4-5 years, respectively, were divided into three groups. The control grou...

Tujuan penelitian ini adalah mengetahui pengaruh ekstrak kedelai untuk meningkatan proliferasi lapisan endometrium dalam proses perkembangan organ uterus pada mencit (Mus musculus) betina. Kelompok kontrol (K) tidak diberi perlukaan. Kelompok P1 diberi ekstrak kedelai dengan dosis 12,5mg/kgBB. Kelompok P2 diberi ekstrak kedelai dengan dosis 25mg/kg...

The purpose of this study was to investigate the effect of sapogenin crude extract of sambiloto (Andrographis paniculata) on creatinine and blood urea nitrogen (BUN) levels and on nephrotoxicity in gentamicin-induced rats. Fifty apparently healthy rats (Rattus norvegicus) were divided equally into five groups: 1) K-, given sapogenin crude extract o...

The aim of this study was to determine if the quality of frozen spermatozoa from goats can be improved by adding bovine seminal protein to sperm diluent. Thirty-six healthy, 3-5 years old, 40-45 kg high-libido goats were used. Seminal protein was obtained from two five-year old Simmental bulls. The 36 goats were equally distributed into three group...

Sapi Rambon merupakan salah satu sapi lokal Indonesia. Tahun 2017 populasi sapi Rambon mengalami penurunan. Salah satu upaya pelestarian sapi lokal adalah melalui peningkatan populasi sapi dan inseminasi buatan. Faktor penentu keberhasilan perkawinan salah satunya adalah libido tinggi dan kualitas semen. Motilitas spermatozoa dan viabilitas spermat...

The aim of this study was to determine the effects of green tea extract as antioxidant in the extender of frozen semen on the viability, motility, integrity of plasma membrane, percentage of necrosis and apoptosis of spermatozoa. Ejaculates collected from four Simmental bulls were evaluated and placed in 37°C water bath before the cryopreservation...

This study aimed to improve the quality of cryopreserved goat semen for artificial insemination by the addition of cattle seminal plasma crude protein. Semen collected from cattle and centrifuged to obtain seminal plasma. Then it was purified to obtain the crude protein. The diluent used in this study was milk-yolk. There were three groups, control...

Nowdays, the storage of buck semen in cold temperature have not satisfied yet because in buck’s seminal plasma contains phospholipase enzyme which can coagulated egg yolk in diluents.The specific aim of this study was to investigate the benefits of L-Arginin amino acid in skim diluents to quality buck’s spermatozoa on cold temperature. This researc...

Pare fruit (Momordica charanthia) potential as an antidiabetic. In preliminary research has proven that alkaloid of the Pare fruit (Momordica charanthia) can lower blood sugar levels of mice suffering from diabetes mellitus. Alkaloid Pare can improve pancreatic β-cell function by improving the preparation phase for the cell dividing (interphase) an...

Sambiloto (Andrographis paniculata L) leaves is tradionally used to maintain health because of it content of androgapholide, an alkaloid that allegedly acted as immunomodulator, either as immunostimulator or as immunosuppresor. The purpose of this study was to prove the effect of Sambiloto alkaloid as immunostimulator on rats (Rattus norvegicus) ba...

Carbon black is one of the components of particulate matter (PM), known as cytotoxic, causing inflammationin the respiratory and cardiovascular system, and able to pass through the placental barrier, which may affect the fetus. The effects caused by pollutants depend on the intensity and time length of the exposure.The objective of this study is to...

BACKGROUND Lead is able to pass through the placental barrier and interfere with fetal development. Red fruit has high antioxidant activity, due to carotenoids (pro-vitamin A), tocopherols (vitamin E) and unsaturated fatty acids. This study aims to examine the effect of red fruit oil towards placenta in pregnant mice before lead exposure. METHODS...

To evaluate the effects of the insulin-like growth factor I (IGF-I) complex from seminal plasma on capacitation, membrane integrity and DNA fragmentation. Methods: A total of 0.5 mL of fresh semen was added to 1 mL of Bracket–Oliphant (BO) medium, and the sample was then centrifuged at a speed of 1800 rpm for 10 min. The samples were analyzed befor...

Penelitian ini bertujuan mengetahui efek sentrifugasi semen domba terhadap persentase kapasitasi dan reaksi akrosom spermatozoa. Penelitian ini terdiri atas 3 kelompok semen domba yaitu kelompok kontrol (P0) semen tanpa sentrifugasi, P1 semen disentrifugasi selama 5 menit dengan kecepatan 1800 rpm, dan P2 semen disentrifugasi selama 10 menit dengan...

Penelitian ini bertujuan mengisolasi protein Insulin Like Growth Factor-I (IGF-I) complex untuk meningkatkan kualitas semen beku kambing pada waktu ekuilibrasi setelah penambahan protein IGF-I complex. Penelitian ini terdiri atas 2 tahap yaitu isolasi protein IGF-I complex dari plasma seminal kambing dan aplikasi terhadap prosesing pembekuan semen....

Series of exploratived laboratory experiment have been conducted to analysed the bioactifity of Insulin Like Growth Factor – I (IGF-I) Complex protein of goat seminal plasm on the membran plasm and acrosom cap of centrifugation sperm. First experiment was conducted to identificatify and isolate IGF –I Complex of goat seminal plasm by using Nati...