Suhas ShindeTexas Tech University | TTU · IGCAST Department of Plant and Soil Science
Suhas Shinde
Ph.D. (Biological and Environmental Science)
About
17
Publications
3,798
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321
Citations
Citations since 2017
Introduction
Additional affiliations
June 2012 - January 2016
Position
- PostDoc Position
Description
- Mapping by sequencing of mutants with altered ProDHpromoter:Luc2 expression to identify regulators and metabolic interactions of stress-induced proline accumulation in A. thaliana.
January 2012 - present
Education
January 2009 - March 2012
Publications
Publications (17)
Phenotype diversity within cultivated Capsicum chinense is particularly evident for fruit shape and size. We used this diversity in C. chinense to further unravel the genetic mechanisms underlying fruit shape variation in pepper and related Solanaceous species. We identified candidate genes for C. chinense fruit shape, explored their contribution t...
Arsenic (As), a non-biodegradable contaminant, is extremely toxic to plants and animals in its inorganic form. As negatively affects plant growth and development, primarily by inducing oxidative stress through redox imbalance. Here we characterized the Arabidopsis F-box protein gene AT2G16220 (Arsenic Stress-Related F-box (ASRF)) that we identified...
Histochemistry is an essential analytical tool interfacing extensively with plant science. The literature is indeed constellated with examples showing its use to decipher specific physiological and developmental processes, as well as to study plant cell structures. Plant cell structures are translucent unless they are stained. Histochemistry allows...
Acid mine drainage (AMD) is a huge environmental problem in mountain-top mining regions worldwide, including the Appalachian Mountains in the United States. This study applied a genome-wide association study (GWAS) to uncover genomic loci in Arabidopsis associated with tolerance to AMD toxicity. We characterized five major root phenotypes—cumulativ...
Jujube (Ziziphus jujuba Mill.), a deciduous tree, is well known for its medicinal and nutritional values. Being an extremophile, it has an excellent capability to survive under arid conditions with limited water availability. In this regard, studying the role of water transport regulating proteins such as Aquaporins (AQPs) in jujube is of great imp...
Metalloids represents wide range of elements with intermediate physio-chemical
properties between metals and non-metals. Many of the metalloids, like boron, selenium, and
silicon are known to be essential or quasi-essential for plant growth. In contrast, metalloids viz.,
arsenic and germanium are toxic to plant growth. The toxicity of metalloids la...
Development of drought-tolerant cultivars is one of the challenging tasks for the plant breeders due to its com- plex inheritance and polygenic regulation. Evaluating genetic material for drought tolerance is a complex process due to its spatiotemporal interactions with environmental factors. The conventional breeding approaches are costly, lengthy...
Silicon is widely recognized as a beneficial element for plant growth. Numerous studies have shown the beneficial effects of silicon, particularly under stress conditions. For the efficient exploration of silicon derived benefits, understanding silicon uptake mechanism, subsequent transport and accumulation in different tissues is essential. Here,...
Watermelon is a good source of citrulline, a non-protein amino acid. Citrulline has several therapeutic and clinical implications as it produces nitric oxide via arginine. In plants, citrulline plays a pivotal role in nitrogen transport and osmoprotection. The purpose of this study was to identify single nucleotide polymorphism (SNP) markers associ...
In plants, change in lipid composition is a common response to various abiotic stresses. Lipid constituents of bryophytes are of particular interest as they differ from that of flowering plants. Unlike higher plants, mosses have high content of very long-chain polyunsaturated fatty acids. Such lipids are considered to be important for survival of n...
Proline accumulation is one of the most prominent changes in plant metabolism during drought and low water potential; however, regulation and function of proline metabolism remain unclear. We used a combination of forward genetic screening based on a Proline Dehydrogenase1 (PDH1) promoter-luciferase reporter (PDH1pro:LUC2) and RNA sequencing of the...
It is widely accepted that atmospheric O2 has played a key role in the development of life on Earth, as evident from the coincidence between the rise of atmospheric O2 concentrations in the Precambrian and biological evolution. Additionally, it has also been suggested that low atmospheric O2 is one of the major drivers for at least two of the five...
In plants, proline synthesis occurs by two enzymatic steps starting from glutamate as a precursor. Some bacteria, including bacteria such as Agrobacterium rhizogenes have an Ornithine Cyclodeaminase (OCD) which can synthesize proline in a single step by deamination of ornithine. In A. rhizogenes, OCD is one of the genes transferred to the plant gen...
The moss, Physcomitrella patens is a non-seed land plant belonging to early diverging lineages of land plants following colonization of land in the Ordovician period in Earth's history. Evidence suggests that mosses can be highly tolerant of abiotic stress. We showed previously that dehydration stress and abscisic acid treatments induced oscillatio...
• Physcomitrella patens is a bryophyte belonging to early diverging lineages of land plants following colonization of land in the Ordovician period. Mosses are typically found in refugial habitats and can experience rapidly fluctuating environmental conditions. The acquisition of dehydration tolerance by bryophytes is of fundamental importance as t...
Questions
Questions (4)
I am characterizing a protein that may interact with proteins involved in trichome development in Arabidopsis. I was wondering whether we can detect these interactions in Y2H assay by using a prey cDNA library generated from one week old Arabidopsis seedling? Or I should use the cDNA library prepared using older seedlings (2-3 weeks)? Any suggestions? Thank you.
We are struggling to control the spider-mites on our Arabidopsis plants. It is important for us to control these mites without damaging our plants.
I will be happy to receive the catalog no and supplier' information!!!
Thank you.
I am purifying His-tagged protein (expected size ~42KDa) using HisExpress™ Protein Purification Column. The protein is expressed in RIL cell line, @17C for 18 hrs. I get protein in a soluble form but I see four clear smaller Kda bands on western blot (with anti-His ab), in fact these small KDa bands are with greater intensity than that of the expected band at ~42 KDa. I used 1X Thermo Scientific™ Halt™ Protease Inhibitor Cocktail but the end result is the same. Can anyone point out whether I am missing something or give suggestions to improve purification? Thank you.
