Sudhirkumar Shinde

• Dr. rer. nat.
• Professor (Associate) at MIT World Peace University

We report the design and synthesis of high affinity and selective sulfonate-binding imprinted polymers using tweezer type functional monomers featuring parallel two imidazolium sites and phenyl sulfonic acid (PSA). The complexation in the pre-polymerization complex and stoichiometry of the bis-imidazolium functional monomer with PSA was evaluated b...

Sphingolipids play crucial roles in cellular membranes, myelin stability, and signalling responses to physiological cues and stress. Among them, sphingosine 1-phosphate (S1P) has been recognized as a relevant biomarker for neurodegenerative diseases, and its analogue FTY-720 has been approved by the FDA for the treatment of relapsing–remitting mult...

Periodontitis (gum disease) is a common biofilm-mediated oral condition, with around 7% of the adult population suffering from severe disease with risk for tooth loss. Moreover, periodontitis virulence markers have been found in atherosclerotic plaque and brain tissue, suggesting a link to cardiovascular and Alzheimer's diseases. The lack of accura...

Immunoaffinity enrichment based on antipeptide antibodies coupled to mass spectrometry-based identification and quantification (immuno-MS) is a promising approach to translate proteomics to clinical assays with diagnostic value. This is linked to precision cancer medicine, where immuno-MS based studies of protein phosphorylation dependent-signallin...

The use of polymerizable hosts in anion imprinting has led to powerful receptors with high oxyanion affinity and specificity in both aqueous and non-aqueous environments. As demonstrated in previous reports, a carefully tuned combination of orthogonally interacting binding groups, for example, positively charged and neutral hydrogen bonding monomer...

Selective serotonin reuptake inhibitors (SSRIs) are a class of antidepressants regularly detected in the environment. This indicates that the existing wastewater treatment techniques are not successfully removing them beforehand. This study investigated the potential of molecularly imprinted polymers (MIPs) to serve as sorbents for removal of SSRIs...

Lipid mediators, small molecules involved in regulating inflammation and its resolution, are a class of lipids of wide interest as their levels in blood and tissues may be used to monitor health and disease states or the effect of new treatments. These molecules are present at low levels in biological samples, and an enrichment step is often needed...

Aberrant glycosylation has been proven to correlate with various diseases including cancer. An important alteration in cancer progression is an increased level of sialylation, making sialic acid one of the key constituents in tumor-specific glycans and an interesting biomarker for a diversity of cancer types. Developing molecularly imprinted polyme...

Sialylations are post-translational modifications of proteins and lipids that play important roles in many cellular events, including cell-cell interactions, proliferation, and migration. Tumor cells express high levels of sialic acid (SA), which are often associated with the increased invasive potential in clinical tumors, correlating with poor pr...

Sphingosine-1-phosphate (S1P) is a bioactive sphingo-lipid with a broad range of activities coupled to its role in G-protein coupled receptor signalling. Monitoring of both intra and extra cellular levels of this lipid is challenging due to its low abundance and lack of robust affinity assays or sensors. We here report on fluorescent sensory core-s...

The design of artificial oxyanion receptors with switchable ion preference is a challenging goal in host-guest chemistry. We here report on molecularly imprinted polymers (MIPs) with an external phospho-sulpho switch driven by small molecule modifiers. The polymers were prepared by hydrogen bond-mediated imprinting of the mono- or di-anions of phen...

Sialylations are post-translational modifications of proteins and lipids that play important roles in many cellular events, including cell-cell interactions, proliferation and migration. Tumor cells express high levels of sialic acid (SA), which are often associated with the increased invasive potential in clinical tumors correlating with poor prog...

The design of hosts for either cations or anions is complicated due to the competition for binding by the host or guest counterions. Imprinting relying on self-assembly offers the possibility to stabilize the guest and its counterion in a favorable geometry. We here report on a comprehensive supramolecular approach to anion receptor design relying...

We report an approach integrating the synthesis of protein‐imprinted nanogels (“plastic antibodies”) with a highly sensitive assay employing templates attached to magnetic carriers. The enzymes trypsin and pepsin were immobilized on amino‐functionalized solgel‐coated magnetic nanoparticles (magNPs). Lightly crosslinked fluorescently doped polyacryl...

Modern healthcare demands rapid and accurate detection of proteins/enzymes at the ultratrace level. Herein we present a molecularly imprinted capacitive sensor for Trypsin, developed by microcontact imprinting. High affinity and selectivity was achieved by doping the prepolymerization mixture with a stoichiometric amount of methacrylamide-based bis...

Phosphate plays a central role in the environment and in biology. This has led to demand for specific phosphate receptor which can be used to sense, enrich or separate phosphate and phosphorylated molecules from complex mixture. Here, we report an approach for the production of phospho-affinity porous beads with controllable size and pore structure...

Molecularly imprinted synthetic receptors are approaching the perfection of natural receptors! In their Communication (DOI: 10.1002/anie.201708635), G. Pan, B. Sellergren, and co-workers report an epitope imprinting strategy for reversible anchoring of the cell-adhesive peptide RGD and demonstrate controllable cell-adhesion behavior.

In this work, an epitope imprinting strategy was employed to dynamically display bioactive ligands on the material interface. An imprinted surface was initially designed to exhibit specific affinity towards a short peptide (i.e., the epitope). This surface was subsequently used to anchor an epitope-tagged cell adhesive peptide ligand (RGD: Arg-Gly-...

In this work, an epitope imprinting strategy was successfully employed to dynamically display bioactive ligands on the material interface. An imprinted surface was initially designed to exhibit specific affinity towards a short peptide (i.e., the epitope). This surface was subsequently used to anchor an epitope-tagged cell adhesive peptide ligand (...

A 2,3-diaminophenazine bis-urea fluorescent probe monomer (1) was developed. 1 responds to phenylphosphate and phosphorylated amino acids in a ratiometric fashion with en-hanced fluorescence accompanied by the development of a red-shifted emission band arising from an excited-state proton transfer (ESPT) process in the hydrogen bonded probe-analyte...

For metal-organic frameworks (MOF), introduction of special functional groups and integration on porous support will endow the MOF with specific molecular affinity and tunable membrane-like surface properties. Herein, we demonstrated a facile interface-induced Zn(II)-ligand-fragment co-assembly strategy to in situ fabricate boronate-based MOF membr...

Rapid and cost-effective analysis of histamine, in food, environmental, and diagnostics research has been of interest recently. However, for certain applications, the already-existing biological receptor-based sensing methods have usage limits in terms of stability and costs. As a result, robust and cost-effective imprinted polymeric receptors can...

Two templating approaches to produce imprinted phosphotyrosine capture beads with controllable pore structure are re-ported and compared with respect to their ability to enrich phosphopeptides from tryptic peptide mixture. The beads were prepared by polymerization of urea-based host monomer and crosslinker inside the pores of macroporous silica bea...

Herein we explore phospholipid imprinting as a means to design receptors for complex glycolipids comprising the toxic lipopolysaccharide endotoxin. A series of polymerizable bis-imidazolium and urea hosts were evaluated as cationic and neutral hosts for phosphates and phosphonates, the latter used as mimics of the phospholipid head groups. The bis-...

This work describes the preparation and investigation of molecularly imprinted polymer (MIP) microgels (MGs) stabilized Pickering emulsions (PE) for their ability to catalyze the formation of disulfide bonds in peptides at the O/W interface. The MIP MGs were synthesized via precipitation polymerization and a programmed initiator change strategy. Th...

The synthesis of uniform molecularly imprinted polymer (MIP) microspheres (MSs) using distillation precipitation polymerization (DPP) at high temperature has attracted great interest in the field of molecular imprinting. However, there are still some shortcomings in this method. In this work, to create uniform MIP MSs in a short time and to demonst...

Sialic acid (SA) is normally expressed on the cell membranes and is located at the terminal position of the sugar chains. SA plays an important role for regulation of the innate immunity, function as markers of the cells and can be recognized by a variety of receptors. Interestingly, the level of SA expression is increased on metastatic cancer cell...

It was concluded that the prepared MIPs and MIM using urea monomer as a functional monomer give high efficiency toward the glyphosate removal even in biological matrix

The expression of cell surface glycans terminating with sialic acid (SA) residues has been found to correlate with various disease states there among cancer. We here report a novel strategy for specific fluorescence labeling of such motifs. This is based on sialic acid imprinted core-shell nanoparticles equipped with nitrobenzoxadiazole (NBD) fluor...

Phosphospecific enrichment techniques and mass spectrometry (MS) are essential tools for comprehending the cellular phosphoproteome. Here, we report a fast and simple approach for low sequence-bias phosphoserine (pS) peptide capture and enrichment that is compatible with low biological or clinical sample input. The approach exploits molecularly imp...

Current mass spectrometry-based lipidomics aims to comprehensively cover wide ranges of lipid classes. We introduce a strategy to capture phospho-monoester lipids and improve the detection of long-chain base phosphates (LCB-Ps, e.g. sphingosine-1-phosphate). Ten novel LCB-Ps (d18:2, t20:1, odd carbon forms) were discovered and characterized in tiss...

Solid-phase extraction (SPE) is one the most common sample preparation techniques in many laboratories. Optimization of analyte specificity and recovery is crucial for high-sensitivity detection and analysis robustness/reproducibility. The optimization process usually starts with screening for a suitable extraction phase for a certain analyte (e.g....

Phosphorylation and Sulfation are important posttranslationally modifications affecting the biological properties of carbohydrates, proteins and glycoproteins. The enzymes responsible, protein kinases and Tyrosylprotein sulfotransferase thus play a key role in the regulation of the cellular processes and communication, e.g. higher tyrosine kinases...

Leaving a lasting impression: Molecularly imprinted polymers (MIPs) featuring neutral binding sites for phosphotyrosine (P) is also shown to have affinity for sulfotyrosine (S) and a sulfotyrosine-containing peptide. The MIPs were capable of selectively capturing both a phosphorylated and a sulfated peptide in a mixture and could release the peptid...

Two molecularly imprinted polymers (MIPs) that we recently described to be class-selective for glucuronides have been successfully exploited for the molecularly imprinted solid-phase extraction (MISPE) of testosterone glucuronide (TG) from its parent drug (T) in urine. Both sorbents targeted the glucuronate fragment but feature different functional...

Two series of molecularly imprinted polymers (MIPs) for the class-selective recognition of glucuronides have been prepared by using lipophilic substructures of the target analyte as template molecule and potent host monomers against oxyanions, that are expected to establish a strong stoichiometric interaction with the single carboxylic group of the...

Novel molecularly imprinted polymers (MIPs) designed to bind the side chain of phosphotyrosine can be used as artificial receptors for affinity-based enrichment of proteolytic peptides. In comparison with general enrichment methods for phosphorylated peptides such as TiO(2)-based methods, the pTyr-imprinted polymers offered high selectivity for pTy...

Hyperphosphorylation at tyrosine is commonly observed in tumor proteomes and, hence, specific phosphoproteins or phosphopeptides could serve as markers useful for cancer diagnostics and therapeutics. The analysis of such targets is, however, a challenging task, because of their commonly low abundance and the lack of robust and effective preconcentr...

This study describes the synthesis of urea formaldehyde (UF) microspheres by a dispersion polycondensation polymerization method. These microspheres with proper F/U molar ratio can provide highly reactive groups, capable of further condensation with the amino acid residues of enzyme/proteins. Presence of methylols groups in UF microspheres was conf...

Polyurethane (PU) microcapsules containing hydrophilic model drug namely Ibuprofen-Na were prepared in non-aqueous medium. Effect of nature of diol (having different number of carbon atoms), used as soft segment in wall forming PU, on release of drug in water was studied. It has been found that as number of carbon atoms in diol increases, release r...