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Introduction
Skills and Expertise
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April 1989 - December 1991
March 2000 - April 2013
May 1998 - February 2000
Publications
Publications (75)
A 60-day study was conducted to evaluate the effect of dietary Moringa oleifera leaf extract (MLE) on growth, enzyme activity and non-specific immune parameters in rohu Labeo rohita. To ascertain this, 180 fingerlings of L. rohita (average weight 12.24±0.5 g) were randomly distributed into four groups based on the level of inclusion of M. oleifera...
The use of chemicals, biologicals and veterinary medicinal products (VMPs) helps in healthy and sustainable fish production. Information on the use of these products is essential for assessing farming practices, potential human health and environmental risks. A questionnaire‐based nationwide survey covering aquaculture farms (n = 2936) producing ca...
The myxovirus resistance (Mx) proteins belong to interferon-induced dynamin GTPase and play pivotal role in the inhibition of replication of numerous viruses. These antiviral proteins are released in usual or diseased condition to prevent the viral attack and to carry regular cellular activities like endocytosis and trafficking of nucleoproteins in...
Background: India has the largest burden of drug-resistant organisms compared with other countries around the world, including multiresistant and extremely drug-resistant tuberculosis and resistant Gram-negative and Gram-positive bacteria. Antibiotic resistant bacteria are found in all living hosts and in the environment and move between hosts and...
A 120‐day feeding trial was conducted to assess the effects of dietary supplementa‐
tion of zinc oxide (ZnO) and selenium (Se) nanoparticles (NPs) on growth, immuno‐
logical and enzymatic profiles in rohu, Labeo rohita. Fishes were fed with basal diet
incorporated with laboratory‐synthesized nanoparticles, viz., zinc oxide nanoparti‐
cles—10 mg/kg...
This study was conducted to evaluate the effect of dietary emamectin benzoate (EB) levels on immune responses, serum enzyme activities, and retention of EB in muscle tissue to establish the withdrawal period in rohu, Labeo rohita juveniles (avg. wt. 18 ± 0:11 g). To ascertain this, 450 healthy L. rohita juveniles were fed with EB in graded doses vi...
This study was conducted to evaluate the effect of dietary emamectin benzoate (EB) levels on immune responses, serum enzyme
activities, and retention of EB in muscle tissue to establish the withdrawal period in rohu, Labeo rohita juveniles (avg. wt. 18 ± 0:11
g). To ascertain this, 450 healthy L. rohita juveniles were fed with EB in graded doses vi...
An experiment of 60 days duration was conducted to evaluate the effect of mono or multi-microbe probiotics on growth, enzymatic profile and immune response in rohu Labeo rohita (Hamilton, 1822) fingerlings. Two hundred-forty numbers of uniformly sized rohu fingerlings were randomly distributed in four treatment groups viz, C (control feed without p...
In spite of vast diverse fish genetic resource of India, several exotic species have been introduced into India. Chinese carps which were introduced in India with an objective of increasing the yields in aquaculture has paved way for composite fish culture in India. Introduction of exotic species for fisheries and aquaculture was successful in some...
The present study was conducted to assess the effect of oxytetracycline hydrochloride (OTC) in rohu, Labeo rohita, with graded doses viz., 80 (1×), 240 (3×), 400 (5×) and 800 (10×) mg kg⁻¹ fish biomass day⁻¹ respectively through feed. Four hundred and fifty healthy rohu juveniles (20 ± 0.12 g) were fed for 30 days followed by 10 days of withdrawal...
In spite of vast diverse fish genetic resource of India, several exotic species have been introduced into India. Chinese carps which were introduced in India with an objective of increasing the yields in aquaculture has paved way for composite fish culture in India. Introduction of exotic species for fisheries and aquaculture was successful in some...
In spite of vast diverse fish genetic resource of India, several exotic species have been introduced into India. Chinese carps which were introduced in India with an objective of increasing the yields in aquaculture has paved way for composite fish culture in India. Introduction of exotic species for fisheries and aquaculture was successful in some...
Abstract
In spite of vast diverse fish genetic resource of India, several exotic species have been introduced into India. Chinese carps which were introduced in India with an objective of increasing the yields in aquaculture has paved way for composite fish culture in India. Introduction of exotic species for fisheries and aquaculture was successf...
An experiment of 60 days duration was conducted to evaluate the effect of mono or multi-microbe probiotics on growth, enzymatic profile and immune response in rohu Labeo rohita (Hamilton, 1822) fingerlings. Two hundred-forty numbers of uniformly sized rohu fingerlings were randomly distributed in four treatment groups viz, C (control feed without p...
Background: India has the largest burden of drug-resistant organisms compared with other countries around the world, including
multiresistant and extremely drug-resistant tuberculosis and resistant Gram-negative and Gram-positive bacteria. Antibiotic resistant bacteria are found in all living hosts and in the environment and move between hosts and...
BACKGROUND
India has the largest burden of drug‑resistant organisms compared with other countries around the world, including multiresistant and extremely drug‑resistant tuberculosis and resistant Gram‑negative and Gram‑positive bacteria. Antibiotic resistant bacteria are found in all living hosts and in the environment and move between hosts and e...
The effects of dietary administration of inorganic zinc (zinc sulphate, ZnSO4) and nano zinc (zinc oxide nanoparticles, ZnO‐NP) were evaluated in rohu, Labeo rohita fingerlings. Fish were fed with a basal diet (Control) supplemented with ZnSO4 (T1, T2 and T3) and ZnO‐NP (T4, T5 and T6) at 10, 20 and 30 mg/kg, respectively, for a duration of 45 days...
Knowledge about the disease process, the pathogen, the host and the environment are very much essential for scientific aquaculture disease management. Strict sanitation of equipment, prevention of entry of other infected fishes in the culture area, prophylactic treatment of pond water checks the spread of many diseases. A holistic approach should b...
Aquaculture has been recognized as an important sector in the world which provides a significant contribution to protein supplement, besides supporting economic development of rural poor. In India, more than 14.5 million people are directly or indirectly dependent on fisheries for their livelihood security and it has been a major driver of socio-ec...
The potential benefits of nanotechnology in intensive aquaculture are considerable. Use of nanoparticles in biomedical and therapeutic applications has opened up a wide area to nanotechnology in the fields like electronics, engineering, medicine, etc. but the possible side effects of nanoparticles have not been much studied hence, detailed study ne...
Rapidly increasing population, rising standards of living and exponential growth of industrialization and urbanization have exposed riverine water resources to various forms of degradation. The deterioration in the water quality impacts the people immediately. In India, during the past five decades, rivers have become rapidly degraded and it is rep...
Aquaculture has become the fastest growing sector of food production in the world. Despite the encouraging trends, several constraints have negative impact on the growth of aquaculture in India as in other parts of the world. In the present survey it was observed that except in some parts in Andhra Pradesh, the farmers involved in aquaculture are m...
The myxovirus resistance (Mx) proteins belong to interferon-induced dynamin GTPase and play pivotal role in the inhibition of replication of numerous viruses. These antiviral proteins are released in usual or diseased condition to prevent the viral attack and to carry regular cellular activities like endocytosis and trafficking of nucleoproteins in...
The effects of silver nanoparticles (Ag-NPs) on the growth, metamorphosis, innate immune parameters and enzyme profiles of tadpole, Polypedates maculatus were studied. Chronic exposure to Ag-NPs for 60 days at 1 mg l-1 and 5 mg l-1 concentrations caused 10% and 50% mortalities respectively alongwith slow growth and delayed metamorphosis as compared...
A 120‐day feeding trial was conducted to assess the effects of dietary supplementation of zinc oxide (ZnO) and selenium (Se) nanoparticles (NPs) on growth, immunological and enzymatic profiles in rohu, Labeo rohita. Fishes were fed with basal diet incorporated with laboratory‐synthesized nanoparticles, viz., zinc oxide nanoparticles—10 mg/kg and Se...
Technical
Rivers Ganga, the largest river system in India, receive millions of litres of sewage, industrial and agricultural wastes, leading to serious water quality and health problems in the towns and villages using these rivers or wetlands as a source of water. Many pathogenic bacteria like Vibrio species, Aeromonas, E. coli, Salmonella, Shigella, Listeri...
Rivers Ganga, the largest river system in India, receive
millions of litres of sewage, industrial and agricultural wastes,
leading to serious water quality and health problems in the
towns and villages using these rivers or wetlands as a source
of water [1]. Many pathogenic bacteria like Vibrio species,
Aeromonas, E. coli, Salmonella, Shigella...
Pamphlet published by ICAR-Central Institute of Freshwater Aquaculture, Kausalyaganga.Odisha.
An investigation on the toxicological effects of Zinc Oxide nanoparticles (ZnO NPs) on tadpoles, Polypedates maculatus in terms of growth, metamorphosis and innate immunity was done. Tadpoles other than those in the control (C) group,were exposed to three different concentrations of ZnO NPs viz., 50 mg/L (T1), 10 mg/L (T2), 1 mg/L (T3) for a period...
Survey was performed to assess the availability and use of different commercial grade aqua-medicines, drugs and chemicals in aquaculture activities in the selected key districts of four aquaculture dominant states of India viz., Andhra Pradesh, Odisha, Jharkhand and Chhattisgarh. A total of 265 farms, 36 aqua shops, and 18 drug manufacturer units w...
Indian fisheries and aquaculture is an important sector of food production, providing nutritional security to the food basket, contributing to the agricultural exports and engaging about fourteen million people in different activities. With diverse aquatic resources the country has shown continuous and sustained increments in fish production since...
Indian fisheries and aquaculture is an important sector of food
production, providing nutritional security to the food basket,
contributing to the agricultural exports and engaging about fourteen
million people in different activities. With diverse aquatic
resources, the country has shown continuous and sustained
increments in fish production since...
Survey was performed to assess the availability and use of different
commercial grade aqua-medicines, drugs and chemicals in
aquaculture activities in the selected key districts of four aquaculture
dominant states of India viz., Andhra Pradesh, Odisha, Jharkhand
and Chhattisgarh. A total of 265 farms, 36 aqua shops, and 18 drug
manufacturer units w...
Indian fisheries and aquaculture is an important sector of food
production, providing nutritional security to the food basket, contributing
to the agricultural exports and engaging about fourteen
million people in different activities. With diverse aquatic resources
the country has shown continuous and sustained increments in
fish production since...
In the present work, we describe the synthesis of gold nanoparticles (AuNPs) using the root and leaf extracts from Vetiveria zizanioides and Cannabis sativa, respectively and its comparison between the two different chemical synthesis methods. UV-visible spectroscopy, particle formation kinetics and scanning electron microscopy (SEM) analyses were...
Rohu Labeo rohita juveniles stunted for 2, 4, 6, 8 10 and 12 months at 20 and 40 m−3 densities were subsequently cultured for 1 year in six grow-out earthen ponds each 0.09 ha. Each pond was stocked with juveniles stunted for a particular duration (treatment) at a combined density of 8,000 ha−1 from two density groups at a 1:1 ratio. Electronic tag...
The aims of this study were to identify alternative myxovirus (Mx) stimulatory compounds in Cirrhinus mrigala and to characterize the kinetics and intensity of their stimulated responses by semi-quantitative RT-PCR. Mx transcripts were measured in C. mrigala injected with Aeromonas OmpC (outer membrane protein) at a dose 0.4 mg/fish. At day 1, day...
Changes in water quality parameters during closed system transport of Labeo rohita fry were studied to identify the critical changes responsible for seed mortality. Fry of L. rohita (2.38 +/- 0.25 g, 44.2 +/- 6.6 mm) were packed in 39 polythene bags and placed on a continuously shaking platform. Water quality changes and fish mortality in the packs...
Comparative study on the effect of nano selenium (nano-Se) and sodium selenite on the growth, bioavailability, antioxidative activities, hematological and biochemical parameters, cellular and humoral immunity was done in layer chicks up to 8th week post feeding. The results showed significant differences (P<0.05) in relative weight gain and final b...
The emergence of diseases and mortalities in aquaculture and development of antibiotics resistance in aquatic microbes, has renewed a great interest towards alternative methods of prevention and control of diseases. Nanoparticles have enormous potential in controlling human and animal pathogens and have scope of application in aquaculture. The pres...
Comparative evaluation of pollution status river Ganga in the present and past has been assessed in the present study. Due to incereased pollution in manifold, worship materials, untreated industrial effluents are being dumped in the holy river at many places and the water flow in the river has been interrupted through the construction of many dams...
Presence of V. cholerae in fish, meat and water samples was verified using standard cultural and T aqMan real-time PCR method. Samples enriched with alkaline peptone water were found more suitable for detection V. cholerae in cultural and real-time PCR methods than non-enriched samples. Out of 52 samples screened, 23 showed positive reaction in rea...
Integrated Fish Farming (IFF) is a sustainable-agriculture technology practiced widely in Asia and other regions of the world. This is very popular, advanced and widely practised in China. Hence most culture methods have either been derived from Chinese methods or modified suitably as per the regional requirements. This integrated technology can of...
Fish and water samples from a sewage-fed bhery, Mathura beel and the Hooghly river were examined for total plate count, total Vibrio, fecal coli forms; E. coli, Aeromonas, Salmonella, faecal Streptococci. Results revealed presence of most of these pathogens in water samples but comparatively low level of pathogens in fish muscle. Fish and water col...
Wastwater aquaculture, as practiced in the East Kolkata Wetlands, has attracted much international attention as a model system for the reuse of urban wastewater and resource recovery. At present the multifunctional wetland ecosystem covers approximately 12,500 ha, and is comprised of mainly of 254 fisheries managed for wastewater aquaculture, agric...
A hydrophila is a potential bacterial pathogen for humans and aquatic animals and is of zoonotic importance. A. hydrophila was isolated from fish, meat and water samples and screened using PCR. A 209 bp DNA product could be amplified in positive cases. RAPD analysis indicated a high degree of genomic variability among A. hydrophila isolates. All A....
Commensalisms or symbiosis or the concept of probiosis is a prospective outcome of the dynamic co evolution of host-bacterial relationships. The use of antibiotics is discouraged in aquaculture as it has led to the appearance of drug-resistant bacteria, immunosuppression in animals besides harmful effects on the environment and concerns on food saf...
V. parahaemolyticus isolates from fish and shrimp P. monodon were screened using PCR and 623 bp product could be visualized in positive cases. The selected isolates were further characterized using protein profile analysis on 11% SDS-PAGE and DNA restriction profile analysis. Variable protein bands on SDS-PAGE indicated antigenic variation among V....
Investigations were carried out to study the present status of prevalence of white spot syndrome virus (WSSV) and monodon baculovirus (MBV) in different prawn farms and natural sources along coastal belt of West Bengal and Orissa, specifically in the areas previously affected with white spot disease (WSD). Both Penaeus monodon and P. indicus cultur...
Penaeus monodon, samples collected from different shrimp farms located in North 24 Parganas, West Bengal, were analyzed for detection of White Spot Syndrome Virus (WSSV) and Vibrio parahaemolyticus. Affected shrimp exhibited reddish body discoloration with necrosis of appendages. Minute white spots or patches on carapace and body cuticle were also...
Prevalence status of white spot disease (WSD), caused by white spot syndrome virus (WSSV) and Monodon baculovirus (MBV), was studied in different prawn farms in North and South-24 paragana districts of West Bengal, specifically in the areas previously affected by WSD. In total 283 samples of giant tiger prawn Penaeus monodon, Indian white prawn P....
Microbiological analysis of samples collected from cases of white spot disease outbreaks in cultured shrimp in different farms located in three regions along East Coast of India viz. Chidambram (Tamil Nadu), Nellore (Andhra Pradesh) and Balasore (Orissa), revealed presence of Vibrio alginolyticus, Vibrio parahaemolyticus, and Aeromonas spp. but exp...
Dot immunasary (DIA) method was used to detect Vibrio alginolyticus and Aeromonas hydrophila in samples collected from infected shrimps Penaeus mondon and fishes Mugil cephalus. Bacterial cultures (OD650 = 0.5) were diluted to 1:40 and 5 μl were spooted onto nitrocellulose paper (NCP). The NCPs were allowed to react separately with antiserum raised...
Fowlpox virus vaccine strain and two field isolates were propagated in chicken embryo fibroblast cell culture. Analysis of viral soluble antigen on 10% SDS-PAGE revealed 10 major polypeptides of MW between 108.25 and 17.80 kDa. More than 30 polypeptides were detected on 12% gel and laser beam scanner identified 26-28 soluble antigens as seperate pe...
Vaccination of poultry against fowlpox has been in practice since long. Although the freqeuncy of out breaks has declined, still reports of disease in vaccinated flocks are of great concern (Dhanesar et al., 1980). Various serological tests viz., immunodiffusion test (Uppal and Nilakantan, 1970), complement fixation test (Harada and Metamato. 1962)...
Fowlpox virus vaccine strain and two field isolates collected from out breaks of disease were purified from cell culture using sucrose gradient ultracentrifugation. The viral DNAs were digested with Bgl I, Bam HI, Hha I and Sma I restriction endonucleases and the fragment pattern was analysed on 0.7% agarose gel. Bgl I digestion produced 54 fragmen...
A fowlpox virus isolate obtained from an outbreak of disease in a vaccinated poultry flock was propagated in chicken embryo fibroblast cell culture. Analysis of purified virus polypeptide on 7.5-15% gradient polyacrylamide gel revealed 45 structural polypeptides after Coomassie blue staining. The mol.wt. of polypeptides ranged between 225.53 and 10...
Chickens infected with fowlpox virus (FPV) IVRI vaccine strain and two field isolates collected from clinical cases of disease (Bareilly isolate and Panchmahal isolate) produced humoral antibody response after 2nd week post-infection, with a noticeable variation in degree of immune response. Serum antibody titre peaked at 4th week post-infection wi...
Structural polypeptides of IVRI vaccine strain and two field isoaltes of Fowlpox virus (Bareilly isolate and Panchmahal isolate) were analysed on SDS-PAGE and by immunoblotting technique. In 5%-20% gradient acrylamide gel 31, 29 and 31 polypeptide bands and in 7.5%-15% gradient gel 45, 37 and 39 polypeptide bands were detected after Coomassie blue...
