Subhasis Batabyal

West Bengal University of Animal and Fishery Sciences | WBUASFSCI · Department of Veterinary Biochemistry

This study depicts the drug-resistance and phylogenomic characteristics of 365 Escherichia coli (EC) and 76 Klebsiella pneumoniae (KP) isolated from stray dogs (293) in and around Kolkata, India. Initial screening found 59 isolates, including 48 E. coli and 11 KP multidrug resistant, which included 33 extended-spectrum β-lactamase, 41 AmpC β-lactam...

Aberration in metabolic homeostasis resulting into under-utilization, over-production, deficiency or excessive storage of certain metabolites may lead to the onset of metabolic disorders. The most prevalent metabolic diseases that occur in livestock are ketosis, parturient paresis, diabetes mellitus, ovine pregnancy toxaemia, hypoglycaemia in baby...

Fowl Pox Viruses (FPV) infect chickens and turkeys giving rise to pock lesions on various body parts like combs, wattles, legs, shanks, eyes, mouth etc. The birds, affected with FPV, also show anemia and ruffled appearance which are clinical symptoms of Reticuloendotheliosis. Interestingly, the field strains of FPV are integrated with the provirus...

The caseins (Alpha s1, Alpha s2, beta and kappa) comprise the major protein component of ruminant milk and are secreted in the form of stable calcium phosphate micelles. The present study was proposed to characterize αs1-casein gene in buffalo at the molecular level to determine complete αs1-casein cDNA sequence. Sequencing results were analyzed an...

Animal manure consists of mainly urine and faeces; additionally, it also contains bedding materials, leftover feed, cleaning water, and other farming wastes. Manure is a good source of plant nutrients which is typically applied to soils as fertilizer by farmers to improve the fertility of the soil. Domestic livestock produces a huge amount of manur...

The present investigation was conducted to explore the effect of Selenium-enriched yeast supplement on the gross morphology and morphometry of the small intestine during post-hatch growth of chicken. Total eighty numbers of day-old broiler chicks were procured from the authorized breeder and were grouped into control and treatment groups. Each grou...

The emergence and spread of carbapenem-resistant Enterobacteriaceae (CRE) are perceived as a serious public-health threat world-wide. Despite sporadic reports, no systemic study has been carried out on CRE in companion animals in Indian subcontinent. In total, 237 canine specimens collected from five veterinary polyclinics in and around Kolkata wer...

The importance of pig as a livestock species contributing to the meat production is well known. However, in recent years, the gut microbiome of pig has received considerable attention, as pigs serve as excellent biomedical models for human health compared to other non-primates. The pig gastro-intestinal tract harbours a lot of microbes, including b...

Pomegranate fruit peel is a great source of natural polyphenols. The objective of the present study was to evaluate efficacy of pomegranate peel infusion (PPI) on growth characteristics, feed efficiency, blood metabolites and antioxidant profile of broiler chicken. A total of 200 broiler chickens were randomly assigned to 4 treatments with 5 replic...

The current situation of the world is seeming to be grave due to the worldwide pandemic of Covid-19 caused by the SARS-CoV-2. The disease has originated from bats and then to pangolins and lastly to the humans. Due to its high contagiousness, it showed a spread to all the countries of the world. The disease comprises of symptoms of Breathlessness,...

The present study was undertaken to investigate genetic variability in a fragment comprising 5′UTR along with partial coding sequence of Hsp70 gene and its association with thermotolerance traits in Murrah buffalo at ICAR-Research Complex for Eastern Region, Patna (India). The allelic variants were identified from genomic DNA samples using SSCP tec...

This study was aimed to genetic profiling of heat shock protein 70 (Hsp70) gene in Murrah buffalo investigating 50 unrelated adult animals at ICAR-Research Complex for Eastern Region, Patna (India) in winter, spring, and summer. PCR ready genomic DNA samples and season-wise total RNA samples were prepared. The PCR products of Hsp70 eluted from agar...

This presentation depict the routine clinical biochemistry uses in disease diagnoses and treatment.

Journal of Animal Health and Production 7(1):21-24. Buffaloes have poor heat tolerance capacity compared to other domestic ruminants and are more prone to heat stress due to scarcely distributed sweat glands, dark body color and sparse hair on the body surface. Thermal environment is a major factor that can affect milk production and reproduction i...

Restoration of the external ear and nose in human patients, in either congenital deformity or acquired defects, is a challenge in reconstructive surgery. Optimization of the currently available materials is necessary for rhinoplasty and microtia correction to avoid intraoperative manoeuvring and early rejection. The aim of this study is to develop...

This study was undertaken to detect the prevalence of CTX-M-producing Klebsiella spp. in healthy broiler, indigenous, and kuroiler birds reared in West Bengal (India) during November 2014-February 2015. In addition to CTX-M gene, the study was also conducted to reveal the occurrence of other β-lactamase and class I integron genes in Klebsiella spp....

Objective: To evaluate the antibacterial property of silver nanoparticles (AgNPs) and capsaicin against multidrug resistant (MDR) and extended spectrum beta-lactamase (ESBL) producing Escherichia coli of bovine and poultry origin. Methods: Antibacterial efficacy of AgNPs and capsaicin was measured using broth dilution method. Five MDR-ESBL producin...

Beta-lactoglobulin (β-lg) is a major whey protein found in cow milk and other ruminants, deer, bison, and buffalo. β-lg is considered as one of the main allergenic components in bovine milk and therefore the modification of β-lg is considered as a promising treatment for milk allergy. Thus the present study was conducted to assess the antigenicity...

The role of oxidative stress in the development of diabetes mellitus and its vascular complications are extensively studied. Hyperglycaemia causes oxidative damage by generation of reactive oxygen species and results in the development of complications. The present study was undertaken with the objective of exploring the anti-hyperglycaemic potenti...

Beta-lactoglobulin (β-lg) is a major whey protein found in cow milk and other ruminants, deer, bison, and buffalo. β-lg is considered as one of the main allergenic components in bovine milk and therefore the modification of β-lg is considered as a promising treatment for milk allergy. Thus the present study was conducted to assess the antigenicity...

Shiga toxin producing (STEC), enteropathogenic (EPEC) and enterotoxigenic Escherichia coli (ETEC) were isolated from 29 diarrhoeic yak calves from two districts of Arunachal Pradesh, India (West Kameng and Tawang) during 2005 to 2011. The STEC (28) and EPEC (12) isolates belonged to 25 different O serogroups. Among the 28 STEC isolates, 8 (28.5%) i...

Reactive oxygen species are increased by hyperglycemia. Hyperglycemia, which occurs during diabetes (both type 1 and type 2) and, to a lesser extent, during insulin resistance, causes oxidative stress. Oxidative stress may be important in diabetes because of its role in the development of diabetic complications. The present study was undertaken wit...

The investigation was carried out to assess the influence of trace mineral supplementation on certain steroid and thyroid hormones that affect reproduction in Black Bengal goat and Garole sheep. Trace mineral feeding to local goat and sheep breeds showed positive influence. Rise of the serum trace minerals does not necessarily influence rising of s...

The present study was undertaken with the objective to evaluate and compare the hypoglycemic and antioxidant potential of crude ethanol extract of the two different plant parts (Barks and Seeds) of Cassia fistula L. in vivo in order to select plant part with better efficacy to continue further studies with semi purified fractions. Albino wistar rat...

The present study was undertaken with the objective to evaluate and compare the hypoglycemic and antioxidant potential of crude ethanol extract of the two different plant parts (Barks and Seeds) of Cassia fistula L. in vivo in order to select plant part with better efficacy to continue further studies with semi purified fractions. Albino wistar rat...

The present work was undertaken to study the comparative phytochemical profiles and hypoglycemic effects of Bauhinia purpurea Linn. Barks (BPBE) and leaves ethanolic extracts (BPLE) in albino wistar rats to validate their ethno medical use in hyperglycemia as well as to explore the better option. Phytochemicals in ethanolic extracts were analyzed b...

In vitro activity of blood and milk leukocytes together with serum enzyme profile during sub-clinical mastitis in crossbred cows were evaluated after collection of blood and milk samples from normal (10) and sub-clinical mastitic (10) cows. Blood total leukocyte counts (TLC) and differential leukocyte counts (DLC) were estimated by standard hematol...

The present study was designed to enumerate the antioxidant status and trace mineral profile of yaks with haemorrhagic diarrhoea associated with enterovirulent E. coli. The study was conducted in 15 yaks with haemorrhagic diarrhoea and with PCR based evidence for enteropathogenic E. coli (EPEC) and enterotoxigenic E. coli (ETEC) infection. A signif...

Aim: The present study was undertaken to standardize a convenient method for isolation and purification of β-lactoglobulin (β-lg) from cow milk keeping its antigenicity intact, so that the purified β-lg can be used for detection of cow milk protein intolerance (CMPI). Materials and methods: Raw milk was collected from Gir breed of cattle reared...

Aim: The present study was carried out to compare the associated role of micro minerals and hormones in repeat breeding animals with the normal crossbred cows. Materials and methods: Blood samples were collected from 10 normal cycling and 10 repeat breeding crossbred cows of Ramakrishna Mission Ashram, Narendrapur to study the plasma mineral pro...

Prevention of surgical site infection and wound dehiscence are imperative and also challenging in clinical practice. This study examines the healing response of laparotomy wounds following application of silver nanoparticles. Dermal fibroblasts were exposed to incremential doses of silver nanoparticles and its effect on collagen synthesis and cytot...

Background Dictamnus dasycarpus is widely used as a traditional remedy for the treatment of eczema, rheumatism, and other inflammatory diseases in Asia. The current study investigates the molecular mechanism of anti-inflammatory action of the ethanol extract of Dictamnus dasycarpus leaf (DE) in lipopolysaccharide (LPS)-stimulated RAW 264.7 cells....

A study was conducted on the dogs with moderate to serious illness coming for treatment at the Dog Ward of West Bengal University of Animal and Fishery Sciences, West Bengal, India, to find out possible correlation between fasting blood sugar level with breed, sex and age of dogs. The study result implicated that the blood sugar level was not havin...

This study was undertaken to observe the prevalence, serogroup, avian pathogenic Escherichia coli (APEC)-associated virulence gene, randomly amplified polymorphic DNA (RAPD) pattern, and antibiotic resistance genes of E. coli in backyard layers and their environment in India. From the 360 samples of healthy layers and their environment, 272 (75.5%)...

The present investigation was carried out to characterize buffalo cyst fluid and germinal membrane antigen by a double immuno diffusion test and immunoelectrophoresis. Attempts were also made to find out utility of antigen B for detection of cystic echinococcosis in buffalo by indirect enzyme linked immunosorbent assay (indirect ELISA). Hyperimmune...

It has been observed that 82 percent of milk–allergic patients are sensitive to beta– lactoglobulin (β–lg), a major milk protein that accounts for approximately 10 to 15 percent of total milk proteins. The modification in β–lg is considered a promising venture to mitigate milk allergies. With the aim of standardizing a convenient method for isolati...

Effect of catching-loading and transport was examined on selective blood biochemical variables (cortisol, glucose, lactate, triglyceride, cholesterol, uric acid, total protein, albumin, globulin, A: G ratio, total red cell count, hemoglobin, H:L ratio, calcium and phosphorus) of 270 Vencobb-400 broilers in Indian climatic condition. Birds were grou...

In total, 363 Escherichia coli were isolated from 165 faecal samples of healthy buffaloes in West Bengal, India. Twenty-four of these isolates (6·61%) were found to carry at least one gene characteristic for Shiga toxin–producing Escherichia coli (STEC). These STEC strains belonged to 13 different O-serogroups. The stx1 gene was present in 23 (95·8...

Blood sugar is considered as one of the predisposing cause of several types of diseases in human being. A study on blood glucose level of the ailing dogs coming for treatment in the Dog Ward and referred for various pathological testing of blood was performed with the assistance of Department of Biochemistry, West Bengal University of Animal& Fishe...

In the present study Immunoglobulin G was purified from serum of Yak by gel filtration chroma-tography on Sephacryl S-200. SDS-PAGE of purified yak IgG showed four major polypeptides of 62.94 kDa, 53.29 kDa 29.22 and 28.21 kDa. The purified Immunoglobulin has been found to be immuno-reactive by DID test and Western Blot analysis when treated agains...

ABSTARCT: In the present study Inhibin was purified from goat ovarian follicular fluid by Gel filtration chromatography on Sephacryl S-200. SDS PAGE of purified inhibin revealed the molecular weight of 55 kDa. The purified follicular fluid had been found to be immunoreactive by DID test and Western Blot analysis when treated against hyperimmune ser...

Coccidiosis has a major impact on poultry industry as it affects broiler and layer birds of all age groups. Caecal coccidiosis, caused by Eimeria tenella is a very devastating enteric disease in broiler, which involves huge economic loss In present study, experimental infective dose of Eimeria tenella isolated from field was determined in broiler c...

The present study was designed for screening polymorphism of known fecundity genes in prolific Indian Bonpala sheep. Employing tetra-primer amplification refractory mutation system PCR, 11-point mutations of BMP1B, BMP15, and GDF9 genes of 97 Bonpala ewes were genotyped. The FecB locus of the BMPR1B gene and two loci (G1 and G4) of GDF9 gene were f...

Mutation studies in different prolific sheep breeds have shown that the transforming growth factor beta super family ligands viz. the growth differentiation factor 9 (GDF9/FecG), bone morphogenetic protein 15 (BMP15/FecX) and associated type I receptors, bone morphogenetic protein receptor (BMPR1B/FecB), are major determinant of ovulation rate and...

The present study revealed that total protein, albumin, globulin, albumin -globulin ratio, acid phosphatase and alkaline phosphatase of small follicles were higher where as sodium concentration was significantly higher in large follicles as compared to small follicles.

To know the dynamic changes in immune-effector activities of Indian major carp, rohu (Labeo rohita) against Aeromonas hydrophila, experimental Motile Aeromonas Septicemia (MAS) was developed in rohu using intraperitoneal injection (0.2ml/fish) with live A. hydrophila at a concentration of 1×10 6 cfu/ml. Dynamic changes in humoral and cellular immun...

The Black Bengal is a prolific goat breed in India. Natural mutations in prolific sheep breeds have shown that the transforming growth factor beta (TGF-�) super family ligands such as growth differentiation factor 9 (GDF9), bone morphogenetic protein 15 (BMP15) and their type I receptor (bone morphogenetic protein receptor, BMPR1B) are crucial for...

The authors describe the serological surveillance of bluetongue virus (BTV) group-specific antibody in goats of the coastal saline (Sunderban) area of West Bengal, India. A recombinant viral protein 7 (rVP7)-based indirect enzyme-linked immunosorbent assay (ELISA) was used to detect the antibody in sera. The bacterially expressed rVP7 was purified...

The present study was carried out on the basis of status of arsenic in soil, drinking water and plants, blood, urine and faeces of animals at arsenic prone zone. Within the ambit with the environment, the examination of animals was taken into consideration. They were screened and categorised on the degree of As toxicity. For field works animals wer...

Haematology and biochemical analysis of arsenic affected cattle revealed lower level of Haemoglobin (8.300 b ± 0.221 g/d1), total erythrocytic count (5.477 b ± 0.096-X10 6 μ1), total leucocytic count (6.025 b ± 0.086 X10 6/μ1), total serum protein (6.230 b ± 0.006 g/d1) and increased levels of blood glucose (50.631 b ± 0.673mg/dl), Aspertate aminot...

Indian Journal of Animal Health 45(2):159-63.

The significance of Cryptosporidium as a causative agent of diarrhea has been assessed in bovine for a period of 2 years. A total of 940 faecal samples (470 samples in each year) both from diarrhoeic and non-diarrhoeic bovine (0-12 months age) were examined during three different seasons (rainy season, summer and winter). Overall Cryptosporidium wa...

Presence of Escherichia coli O157 was observed in 3.7% of freshly dressed beef, 3.12% of raw milk and 2.04% of pasteurized milk samples, but not in buffalo meat, goat meat, sheep meat and chicken. All the strains carried Shiga toxin 2 gene and expressed enterohaemolysin and were potentially dangerous for human health. Detection of Shiga toxin-posit...

The immunodominant and species-specific antigen (s) of Fasciola gigantica of diagnosis were prepared by column chromatographic techniques. On gel filtration chromatography, 2 prominent peaks (GP1 and GP2) were obtained. By anion exchange chromatography these fractions were further resolved into P 1D 1, P 1D 2, and P 1D 3 and P 2D 1, P 2D 2, and P 2...

Haematologlcal and biochemical blood analysis of pure breed, farm managed Garole sheep was performed in order to obtain a clear picture of their normal haematological and blood biochemical profile, Experimental animals were chosen from the clinically normal, adult (E months-3 years of age) male and female sheep. Results obtained were compared with...

In the present study, sheep crude hydatid cyst fluid antigen (SCHCFA) was prepared by ammonium sulfate precipitation, centrifugation and dialysis. SDS-PAGE of SCHCFA showed seven polypeptides of 72.8 kDa, 66.5 kDa, 60.2 kDa, 41 kDa, 25.1 kDa, 19 kDa and 8 kDa when stained with Coomassie briliant Blue R-250. The initial part of the ascending loop of...