Subhas C Kundu

Subhas C Kundu
University of Minho · 3B's Research Group and I3Bs - Research Institute on in Biomaterials, Biodegradables and Biomimetics

PhD

About

338
Publications
83,343
Reads
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Citations
Introduction
Subhas C Kundu, Research Coordinator, 3Bs Research Group. I3Bs - Research Institute on Biomaterials, Biodegradables and Biomimetics, University of Minho, Portugal. Present area of research is mainly on 3D in vitro cancer models for drug screening. He was a Professor and First Head, Department of Biotechnology, Indian Institute of Technology Kharagpur and had have worked on natural silk proteins (fibroin and sericin) based bimaterial matrices for TERM.
Additional affiliations
September 2016 - present
University of Minho
Position
  • Chair
September 2016 - June 2020
University of Minho
Position
  • Chair
September 2016 - December 2020
University of Minho
Position
  • Chair

Publications

Publications (338)
Article
Silk fibroin (SF) is a promising biomaterial due to its good biocompatibility, easy availability, and high mechanical properties. Compared with mulberry silk fibroin (MSF), nonmulberry silk fibroin (NSF) isolated from typical nonmulberry silkworm silk exhibits unique arginine-glycine-aspartic acid (RGD) sequences with favorable cell adhesion enhanc...
Article
Silkworm silk protein fibroin and spider silk spidroin are known biocompatible and natural biodegradable polymers in biomedical applications. The presence of β-sheets in silk fibroin and spider spidroin conformation improves their mechanical properties. The strength and toughness of pure recombinant silkworm fibroin and spidroin are relatively low...
Article
Background: Several factors like three-dimensional microstructure, growth factors, cytokines, cell-cell communication, and coculture with functional cells can affect the stem cells behavior and differentiation. The purpose of this study was to investigate the potential of decellularized placental sponge as adipose-derived mesenchymal stem cells (A...
Article
Microfluidic platforms represent a powerful approach to miniaturizing important characteristics of cancers, improving in vitro testing by increasing physiological relevance. Different tools can manipulate cells and materials at the microscale, but few offer the efficiency and versatility of light and optical technologies. Moreover, light‐driven tec...
Article
One of the leading causes of death globally, especially in underdeveloped countries, is bacterial infection. Recently, the prevalence of infections from antibiotic‐resistant bacteria has been increasing, which makes the need for innovative antibacterial wound dressings urgent. We report that g‐C3N4‐based flat silk cocoons (FSCs) with rechargeable a...
Article
The traditional method of injecting insulin requires multiple injections to stimulate the insulin secretion pattern physiologically, and injections have compliance and comfort issues. Microneedle s can reduce pain and are a patient-friendly technique. In this study, silk fibroin was utilized as a drug delivery system, which exhibited an excellent t...
Article
Skin injuries, especially chronic wounds, remain a significant healthcare system problem. The number of burns, diabetic patients, pressure ulcers, and other damages is also growing, particularly in elderly populations. Several investigations have been pursued in designing more effective therapeutics for treating different wound injuries. These effo...
Book
This book offers a comprehensive overview of the development and application of microfluidics and biosensors in cancer research, in particular, their applications in cancer modeling and theranostics. Over the last decades, considerable effort has been made to develop new technologies to improve the diagnosis and treatment of cancer. Microfluidics h...
Chapter
Microfluidics and biosensors have already demonstrated their potential in cancer research. Typical applications of microfluidic devices include the realistic modeling of the tumor microenvironment for mechanistic investigations or the real-time monitoring/screening of drug efficacy. Similarly, point-of-care biosensing platforms are instrumental for...
Chapter
Early cancer detection is still a major clinical challenge. The development of innovative and noninvasive screening approaches for the detection of predictive biomarkers indicating the stage of the disease could save many lives. Traditional in vitro and in vivo models are not adequate to copycat the native tumor microenvironment and for the discove...
Chapter
Early cancer screening and effective diagnosis is the most effective form to diminish the number of cancer-related deaths. Liquid biopsy constitutes an attractive alternative to tumor biopsy due to its non-invasive nature and sample accessibility, which permits effective screening and patient monitoring. Within the plethora of biomarkers present in...
Chapter
Despite the significant amount of resources invested, cancer remains a considerable burden in our modern society and a leading cause of death. There is still a lack of knowledge about the mechanistic determinants of the disease, the mechanism of action of drugs, and the process of tumor relapse. Current methodologies to study all these events fail...
Article
Full-text available
Osteomyelitis is an inflammation of the bone caused by bacterial infection. It usually develops from broken bones, decayed teeth, or heavily punctured wounds. Multi-drug-resistant bacteria are the major hurdle in the treatment of osteomyelitis. The ever-rising antibiotic resistance even leads to amputations or fatalities as a consequence of chronic...
Article
An important hallmark in cancer research is the discovery of suitable features capable to reliably predict tumor invasiveness, and consequently, their metastatic potential at an early stage. Current methods are based on molecular biomarker screening and imaging that may not reveal the altered properties of tumor cells, being also labor-intensive an...
Article
Thermosensitive nanoparticles with phase transition abilities upon temperature change have been considered suitable materials in biomedical fields, especially thermo-sensitive drug delivery systems. Moreover, electroactive injectable hydrogel supporting bone regeneration of the elderly will highly be desired in bone tissue engineering applications....
Article
Full-text available
In vitro cancer models are envisioned as high-throughput screening platforms for potential new therapeutic discovery and/or validation. They also serve as tools to achieve personalized treatment strategies or real-time monitoring of disease propagation, providing effective treatments to patients. To battle the fatality of metastatic cancers, the de...
Article
Extracellular vesicles (EVs) are small lipidic particles packed with proteins, DNA, messenger RNA and microRNAs of their cell of origin that act as critical players in cell-cell communication. These vesicles have been identified as pivotal mediators in cancer progression and the formation of metastatic niches. Hence, their isolation and analysis fr...
Chapter
The constant development of optical techniques for medical diagnostics and therapies has led to a growing need to develop and optimize biophotonic applications. Through biophotonics, it is possible to precisely control light propagation, scattering, and emission, enabling a different range of applications, from light-activated therapies to sensing...
Article
Background Synthetic tissue engineering scaffolds has poor biocompatiblity with very low angiogenic properties. Conditioning the scaffolds with functional groups, coating with biological components, especially extracellular matrix (ECM), is an excellent strategy for improving their biomechanical and biological properties. Methods In the current st...
Article
Full-text available
As a patient-friendly technology, drug-loaded microneedles can deliver drugs through the skin into the body. This system has broad application prospects and is receiving wide attention. Based on the knowledge acquired in this work, we successfully developed a melatonin-loaded microneedle prepared from proline/melatonin/silk fibroin. The engineered...
Article
Full-text available
The emerging field of tissue engineering and regenerative medicines utilizing artificial polymers is facing many problems. Despite having mechanical stability, non-toxicity, and biodegradability, most of them lack cytocompatibility and biocompatibility. Natural polymers (such as collagen, hyaluronic acid, and others), including blends, are introduc...
Article
Despite significant achievements in the understanding and treatment of cancer, it remains a major burden. Traditional therapeutic approaches based on the ‘one-size-fits-all’ paradigm are becoming obsolete, as demonstrated by the increasing number of patients failing to respond to treatments. In contrast, more precise approaches based on individuali...
Article
The efficacy of current therapies for skeletal muscle disorders/injuries are limited urging the need for new treatments. Skeletal muscle tissue engineered platforms represent a promising tool to shed light on the pathophysiology of skeletal muscle disorders/injuries and to investigate the efficacy of new therapies. Herein, we developed a skeletal m...
Article
The generation of invasive fluctuating protrusions is a distinctive feature of tumor dissemination. During the invasion, individual cancer cells modulate the morphodynamics of protrusions to optimize their migration efficiency. However, it remains unclear how protrusion fluctuations govern the invasion of more complex multi-cellular structures, suc...
Article
Full-text available
Chemotherapeutic resistance is a major problem in effective cancer treatment. Cancer cells engage various cells or mechanisms to resist anti-cancer therapeutics, which results in metastasis and the recurrence of disease. Considering the cellular heterogeneity of cancer stroma, the involvement of stem cells is reported to affect the proliferation an...
Article
Rheumatoid arthritis (RA) is an autoimmune and chronic inflammatory disease characterized by joint inflammation. Since the inflammatory condition plays an important role in the disease process, it is important to develop and test new therapeutic approaches that specifically target and treat joint inflammation. In this study, a human 3D inflammatory...
Article
Full-text available
There has been growing interest in the use of natural bionanomaterials and nanostructured systems for diverse biomedical applications. Such materials can confer unique functional properties as well as address concerns pertaining to sustainability in production. In this work, we propose the biofabrication of micropatterned silk fibroin/eumelanin com...
Article
Green solvents such as ionic liquids (ILs) unlock possibilities for developing innovative biomedical and pharmaceutical solutions. ILs are the most investigated solvents for compound extractions, as reaction media and/or catalysts, and a desired eco-friendly solvent to process biomacromolecules for biomaterial production. Investigations demonstrate...
Article
Front Cover: This picture shows the in situ anti‐tumor therapeutic features of the developed calcium‐containing silk fibroin hydrogels cross‐linked in the presence of calcium peroxide and horseradish peroxidase. These are characterized by the fast gelation, compacted porous microstructure and slow drug release profile. They induced the death of enc...
Article
Decellularization, preservation protocol and storage time influence the biomechanical and biological properties of allografts and xenografts. Here, we examined the consequences of storage time on the antibacterial, angiogenic and biocompatibility properties of the decellularized placental sponge (DPS) in vitro and in vivo. The DPS samples were pres...
Article
Hydrogels, being capable of mimicking the extracellular matrix composition of tissues, are greatly used as artificial matrices in tissue engineering applications. In this study, the generation of horseradish peroxidase (HRP)‐crosslinked silk fibroin (SF) hydrogels, using calcium peroxide as oxidizer is reported. The proposed fast forming calcium‐co...
Article
Globally, skin repair costs billion dollars per annum. Diversified matrices are fabricated to address this important area of healthcare. Most common limitations associated with them are the inflated production cost and insufficient functional repair. Our work explores the fabrication and potential utilization of Antheraea mylitta silk protein seric...
Article
Full-text available
Breast cancer is one of the leading causes of mortality worldwide being the most common cancer among women. Despite the significant progress obtained during the past years in the understanding of breast cancer pathophysiology, women continue to die from it. Novel tools and technologies are needed to develop better diagnostic and therapeutic approac...
Chapter
Understanding the pathogenesis of viral infection is of paramount importance for the development of better therapies. In the particular case of COVID-19, the mechanism of infection is highly complex and involves a critical cascade of events, which can lead to the death of the patient. Intense research is currently being performed to gain mechanisti...
Article
Inducing thermal gradients in two injected fluid systems results in the temporal formation of mixing conductive streams. If preserved through sol-gel transition, this mechanism can be used to drive and pat- tern non-living and living entities in mixed hydrogels. Interfaces are vital in nature, where gradients of non-living and living entities build...
Article
Full-text available
Flat cell cultures or xenografts are inadequate tools to unravel cancer complex biology. 3D in vitro tumor models garnered interest since they recapitulate better dynamic mechanisms of cancer, but a gold standard model that faithfully mimics solid cancer is not available yet. 3D breast cancer model is fabricated using freeze-dried silk fibroin scaf...
Article
The concepts and frameworks of soft matter physics and the laws of thermodynamics can be used to describe relevant developmental, physiologic, and pathologic events in which directed cell migration is involved, such as in cancer. Typically, this directionality has been associated with the presence of soluble long-range gradients of a chemoattractan...
Article
Full-text available
Silk protein fibroin (SF)-based matrices from non-mulberry, and mulberry silkworms are used for different applications in regenerative medicine. Silk fiber spun by the wild non-mulberry silkworm Antheraea pernyi (Ap) is also a promising biomedical material, due to the presence of the inherent tripeptide sequence of Arginine-Glycine-Aspartic acid (R...
Article
Cancer metastasis is a highly complex and multistep process, which is initiated by the invasion of tumor cells into the microcirculation system. A diverse variety of organ‐on‐chip models are described investigating this critical event. However, most of these models solely integrate the blood vasculature and overlook the fundamental role of the lymp...
Article
To reduce the pain caused by subcutaneous injections the microneedle patches as the new transdermal drug delivery method are gaining increased attention. In this study, we fabricated a composite insulin-loaded microneedle patch. Silk fibroin, a natural polymer material, was used as the raw material. The tip of the microneedle had good dissolving pr...
Chapter
Traditional in vitro and in vivo models typically used in cancer research have demonstrated a low predictive power for human response. This leads to high attrition rates of new drugs in clinical trials, which threaten cancer patient prognosis. Tremendous efforts have been directed towards the development of a new generation of highly predictable pr...
Book
Edited by David Caballero, Subhas C. Kundu, Rui Luís Reis Volume 156, Pages 2-332 (2020)
Article
Full-text available
Burn injuries represent a major life-threatening event that impacts the quality of life of patients and places enormous demands on the global health care systems. This study introduces the fabrication and characterization of a novel wound dressing made of core–shell hyaluronic acid–silk fibroin/zinc oxide (ZO) nanofibers for treatment of burn injur...
Article
Electrospinning is well thought of as the most potent nanofiber producing technique, which is applicable in biomedical fields. The generation of electrospun nanofibers as drug carriers has widely been shown much interest over the past years. Electrospun nanofibers meet various advantages as drug delivery platforms including high surface area, accep...
Chapter
Cancer remains a major burden in our modern society. A significant amount of resources and efforts is invested in new approaches to better understand and treat this disease. During the last years, the development of novel biomaterials with advanced capabilities, together with modern tissue engineering techniques and nanotechnology tools permits a s...
Chapter
Full-text available
Cancer cells show different behaviors in two-dimensional (2D) and three-dimensional (3D) culture systems or in vivo. These differences stem from a variety of factors, such as the absence of important biological determinants, physical cues, and chemical composition of the environment. All of these factors affect the shape, motility, morphology, cont...
Chapter
Cancer is one of the mortal diseases globally. While considerable advances in its detection and monitoring have been accomplished, simple and continuous cancer monitoring is not yet a reality. Nowadays, a cancer diagnosis is based on image analysis techniques, biopsy, and whole blood sample analyses for specific biomarkers. This entire process tend...
Chapter
The biomaterials-based three-dimensional (3D) cancer models enhance the investigation of the mechanisms underpinning the cancer progression and metastasis. In vitro 3D tumor models could substitute the use of two-dimensional cell culture and reduce the number of animals recruited for the preclinical trials of anticancer drug. Moreover, pharmaceutic...
Chapter
Metastasis in biomaterials is a current catchword reflecting the recent efforts of using biomaterials in modeling the multistep process of metastasis. Given that the most cancer-associated causalities are due to metastasis, several model systems are developed to represent the diverse stages of the process, improving our understanding of the disease...
Chapter
Cancer remains a major burden in our modern society, being the second leading cause of death worldwide. Many efforts are being made to improve our knowledge about the etiology of the disease, its early diagnosis, and treatment. Innovative technologies capable of early detection, diagnosis and monitoring the progression of cancer, as well as the eff...
Article
Cancer is a multifaceted pathology, where cellular and acellular players interact to drive cancer progression and, in the worst-case, metastasis. The current methods to investigate the heterogeneous nature of cancer are inadequate, since they rely on 2D cell cultures and animal models. The cell line-based drug efficacy and toxicity assays are not a...
Article
Mineralization is a natural process leading to the formation of mineralized tissue such as bone. The chief mineral component of bone is hydroxyapatite (HAp), which is deposited using an organic template like fibrillar Collagen I under physiological condition. Fibrous silk fibroin is structurally homologous to collagen and acts as nucleation site fo...
Article
Tissue engineering and regenerative medicine (TERM) are paving the way to the generation of functional and mature biological tissues that closely emulate cellular, biochemical, and mechanical cues. Electrical fields in the human body modulate myriad biological processes, such as synapses, muscle contraction, hearing, and wound healing, which were d...
Article
Several synthetic and natural materials are used in soft tissue engineering and regenerative medicine with varying degrees of success. Among them, silkworm silk protein fibroin, a naturally occurring protein-based biomaterial, exhibits many promising characteristics such as biocompatibility, controllable biodegradability, tunable mechanical propert...
Article
The shortcomings of the conventional wound closures like poor wet adhesion strength cause a hydration-persuaded softening, dissolution and viscosity of the adhesives. This happens especially due to the changes in viscosity of the adhesive during underwater surgical operations, which drive the research attention on artificial biomedical adhesives. T...
Article
Peptides have recently emerged as a promising class of targeting ligands for specific drug delivery in cancer treatment, which avoid undesirable side effects of the systemic administration of chemotherapeutics. Their conjugation with nanoparticles has been demonstrated to improve the functionality of peptides resulting in a versatile platform for b...
Article
The damage to the skin is most prominent and evident as it is our first line of defense and unremittingly under attack by biological and environmental factors. The restoration of the skin is dependent on the extent of the injury. To explore the prospects of new biomimetic material, bi-layered skin construct is fabricated in vitro with nonmulberry s...
Article
Full-text available
Osteosarcoma is one of the most common metastatic bone cancers, results in significant morbidity and mortality. Unfolding of effectual therapeutic strategies against osteosarcoma is impeded due to the absence of adequate animal models, which can truly recapitulate disease biology of humans. Tissue engineering provides an opportunity to develop phys...
Article
Cardiovascular diseases, cancer, and diabetes are high mortality diseases, which account for almost two thirds of all deaths worldwide. Their early detection and continuous evaluation are fundamental for an improved patient prognosis and reduced socioeconomic impact. Current biosensor technologies are typically based on the analysis of whole blood...
Article
Regenerating chondral and osteochondral injuries is a main challenge in orthopedics. Some therapeutic strategies such as joint preservation operations, non-operative management, palliative surgery, and arthroplasty are the common clinical methods for repairing the cartilage defects. These treatments show often satisfactory as short-term outcomes an...