Subba Rao Kalluri

Subba Rao Kalluri
University of Hyderabad | UoH · Department of Biochemistry

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33
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Publications

Publications (33)
Article
Full-text available
It is well established now that dietary calorie restriction (CR) leads to extension of life span in many species, although the exact mechanism of this effect is still eluding. In the present study, we examined the effect of 40 % CR imposed during a prolonged period of life span (from 6 to 30 months) of rats on the activity of DNA polymerase β (pol...
Chapter
During evolution, organisms have developed a number of strategies to keep the structure of their genetic material intact. However, evolutionary forces keep driving metabolic changes that would lead to more viable progenies for survival and propagation. Thus natural evolution uses two opposing forces to improve the genetic makeup of the species. Wit...
Article
Topoisomerase IIβ (TopoIIβ), an enzyme involved in DNA rearrangements, is predominantly present in brain and its levels are shown to decrease with age. This study characterizes the function of TopoIIβ in regulating BER (base excision repair) activity. TopoIIβ deficient granule neurons (CGNT̄) show greater sensitivity to N- ethyl N-nitroso urea (ENU...
Article
Fidelity of DNA polymerases is vital for maintaining genomic integrity. Deficient DNA repair leads to age related disorders or cancer. If the age at which the decline in activity of predominant DNA repair enzymes starts is identified, and the deficient proteins supplemented, then the manifestation of these diseases can be delayed promoting healthy...
Article
Adult Wistar NIN (WNIN) rats (6 months old) of both sexes were orally fed Amalakirasayana at a dose of 4.5 g per kg body weight, five days in a week. The Amalakirasayana was prepared by Arya Vaidya Sala, Kottakkal, Kerala, India, which is considered as gold standard. After 3, 9 and 15 months of such therapeutic regime, rats were sacrificed and vari...
Article
Earlier we have used biochemical approach to assess the number of single (SSBs) and double (DSBs) strand breaks in brain cellular DNA. However, a quick method to obtain a reliable measure of DNA damage in cells was in need for population studies. Therefore, single cell gel electrophoresis technique (popularly known as "comet" assay) has been standa...
Article
Free radicals are produced in cells by cellular metabolism and by exogenous agents. These species react with biomolecules in cells and one of the important targets is DNA. This kind of damage, often referred to as oxidative DNA damage, has consequences in various organs and particularly in brain, in view of its high metabolic activity and oxygen co...
Article
Some drugs are routinely prescribed for dementia that sets in either due to normal ageing or due to neurodegenerative disorders. We have studied the effect of three of these drugs, Donepezil hydrochloride, Rivastigmine tartrate and Nootropyl, on the activity of DNA polymerases β, a crucial enzyme in the base excision repair pathway, the most import...
Article
In this Review, familial and sporadic neurological disorders reported to have an etiological link with DNA repair defects are discussed, with special emphasis placed on the molecular link between the disease phenotype and the precise DNA repair defect. Of the 15 neurological disorders listed, some of which have symptoms of progeria, six--spinocereb...
Article
Linearised pUC 19 DNA with cohesive, blunt and non-matching ends, generated by prior treatment with different restriction enzymes was presented as substrate to measure the NHEJ activity to repair DNA double strand breaks in extracts prepared from isolated neurons from neonatal, young adult and old rat cerebral cortex. Highest end joining activity w...
Article
A three-step reaction strategy has been developed to examine the mechanism of extension of a mismatched primer in an oligoduplex substrate by rat neuronal extracts and DNA polymerase beta. The results revealed that in the case of duplexes with a mismatch at 3'-end of primer, significant extension by DNA polymerase beta has taken place only after th...
Article
It is now well established, in many species, that dietary calorie restriction confers beneficial effects like slowing down many age dependent processes and extending the lifespan. There are indications that this phenomenon may be applicable even in non-human primates and humans. However the precise mechanism through which these effects are achieved...
Article
The relative proportions of DNA-polymerases alpha, beta, delta and epsilon (pols alpha, beta, delta and epsilon ) activities in isolated neuronal and astroglial cell fractions from developing, adult and aging rat brain cerebral cortex, were examined. This was achieved through a protocol that takes advantage of the reported differential sensitivitie...
Article
The relative proportions of DNA‐polymerases α, β, δ and ε (pols α, β, δ and ε) activities in isolated neuronal and astroglial cell fractions from developing, adult and aging rat brain cerebral cortex, were examined. This was achieved through a protocol that takes advantage of the reported differential sensitivities of different DNA‐polymerases towa...
Article
A close relationship between the DNA repair potential of various organisms and their rate of aging has been long suspected. We have been looking into the steps of the DNA repair process in isolated neurons from rats of different ages. Unscheduled DNA synthesis (UDS) was low in aging neurons, and also the response of these cells to raise their DNA r...
Article
Synthetic staggered oligodeoxynucleotide duplexes are formed by annealing a 5′-32P-labeled 14-mer with four different 21-mers. These duplexes have either a correct or mismatched base pair at 3′-end of the primer. With these model template primers the ability of neuronal extracts, obtained from rats of different ages, to extend the primer to the pre...
Article
Clinically healthy subjects of the Indian population were divided into three age groups: young, 8–14 years; adult, 20–35 years; old, ≥55 years and were further classified based on body mass index (BMI) as normal BMI (NBMI) ≥20 and low BMI (LBMI) between 16 and 18, respectively. The ability of the peripheral blood lymphocytes from these subjects to...
Article
Down's syndrome (DS) cases from 1–40 years of age and showing no other anomalies or deficiencies were categorized into three age groups: group 1, ⩽12 years; group 2, 13–25 years; and group 3, ⩾26 years. The DNA-repair markers like unscheduled DNA synthesis (UDS), activities of DNA polymerases, (Total, β and ε) and two endodeoxyribonucleases, (UV- a...
Article
Our study reveals the presence of DNA polymerases δ and e, participating in DNA replication and repair, along with already known polymerases α and β, in the developing and aging rat brain. This was achieved through a protocol that takes advantage of the reported differential sensitivities of different DNA polymerases towards certain inhibitors such...
Article
Caloric restriction (CR), has been shown to extend life span and delay both aging and the onset of a number of life-shortening diseases in laboratory animals. Defective DNA repair and DNA damage have been implicated as causal factors in many of these disease processes. CR appears to increase genetic stability by enhancing the DNA repair capacity an...
Article
Two biochemical strategies using nick translation-type of incubation and terminal transferase-catalyzed reaction were used to assess single- (SSB) and double-strand (DSB) breaks in DNA of permeabilized neurons isolated from young, adult, and old rat cerebral cortex. Both SSBs and DSBs accumulate with age. On prior treatment of neuronal cells with 1...
Article
A deoxyribonuclease has been purified to electrophoretic homogeneity from young and old rat brain. The enzyme is an endonuclease, with an optimum pH 5.0. Divalent cations are not needed for the activity. The DNase showed highest activity towards Native DNA either as such or UV irradiated with little activity on denatured DNA, apurinic DNA or DNA pr...
Article
A brief review of the available information concerning age-related genomic (DNA) damage and its repair, with special reference to brain tissue, is presented. The usefulness of examining the validity of DNA-damage and repair hypothesis of aging in a postmitotic cell like neuron is emphasized. The limited number of reports that exist on brain seem to...
Article
The usefulness of conducting DNA damage and repair studies in a postmitotic tissue like brain is emphasized. We review studies that use brain as a tissue to test the validity of the DNA damage and repair hypothesis of aging. As far as the accumulation of age dependent DNA damage is concerned, the data appear to overwhelmingly support the hypothesis...
Article
“Bound’ and “free’ RNA polymerase activities were assessed in the nuclear fraction of cerebral cortical, neuronal, astroglial, and oligodendroglial cells obtained from rats of young, adult, and old ages. Significant decreases in both the bound and free polymerase II activities were noticed in old brain, as compared to adult brain, in neuronal and o...
Article
Hydroxyurea, when injected intraperitoneally at a dose of 1 mg/g body weight, inhibited thymidine kinase activity in developing rat cerebrum (16-day-embryonic) and cerebellum (7-day-postnatal) within a few hours of administration. The inhibition was time-dependent and both cytosolic and mitochondrial thymidine kinases were affected. Under the same...
Article
: Neurons do not divide during adult life and thus they provide a unique system to study the effects of age-accumulated damage to DNA in the absence of DNA replication. We have analyzed DNA polymerase activity in neurons isolated from young adult and very aged mice. The predominant catalytic activity is DNA polymerase-β and it is present in similar...
Article
Hydroxyurea, when injected intraperitoneally, exerted marked inhibition on the activity of thymidine kinase in 5 day old postnatal cerebellum and 15 day old embryonic cerebrum. However, it failed to show any sustained inhibition on thymidine kinase activity in 5 day old postnatal cerebrum. In this case, the marginal decrease of thymidine kinase act...
Article
Full-text available
The effect of early postnatal undernutrition and subsequent rehabilitation on wet weight, DNA, RNA, protein and the activities of acid and alkaline DNases in the cerebellar region of rat brain was studied. The cerebellar region was found to be affected significantly during early undernutrition. Further, earlier the initiation of nutritional rehabil...

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