Stratis Kolibianakis

Stratis Kolibianakis
Aristotle University of Thessaloniki | AUTH · 1st Dept of Obstetrics and Gynaecology, Unit for Human Reproduction

MD MS PhD

About

273
Publications
26,673
Reads
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11,762
Citations
Citations since 2016
69 Research Items
5260 Citations
20162017201820192020202120220200400600800
20162017201820192020202120220200400600800
20162017201820192020202120220200400600800
20162017201820192020202120220200400600800
Introduction
Skills and Expertise
Additional affiliations
June 2006 - present
Aristotle University of Thessaloniki
Position
  • Clinical Director
June 2006 - present
Aristotle University of Thessaloniki
Position
  • Professor (Assistant)
June 2006 - present
Aristotle University of Thessaloniki
Position
  • Professor (Assistant)

Publications

Publications (273)
Article
Frozen embryo transfer is an established, highly successful technology in Assisted Reproductive Technologies. Its use as a part of a freeze only strategy is associated with a higher probability of live birth in high responders and a decrease incidence of ovarian hyperstimulation syndrome (OHSS). Its use in normal responders, maintains the probabili...
Article
A systematic review and meta-analysis was performed aiming to identify good quality randomized controlled trials (RCTs) evaluating testosterone pretreatment in poor responders. Eight RCTs were analyzed, evaluating 797 women. Transdermal testosterone gel was used in all studies with a dose ranging from 10 to 12.5 mg/day for 10 to 56 days. The main o...
Article
Research question Are oxytocin and oxytocin receptor expressed in human spermatozoa and is their expression different between normal semen samples and samples with at least one abnormal parameter? Design An in-vitro prospective study was performed, including a total of 175 semen samples from Greek men. Semen analysis was performed according to Wor...
Article
Study question Can automated measurements of follicular volume by three-dimensional (3D) ultrasound predict the number of mature oocytes retrieved better than two-dimensional (2D) measurements of follicles? Summary answer Automated measurements of follicular volume by 3D ultrasound have similar predictive ability of the number of mature oocytes re...
Article
Study question Does the number of follicles ≥11mm differ when measured by manual-2D or automated-3D ultrasound and which method predicts the number of oocytes collected more accurately? Summary answer Manual-2D and automated-3D ultrasound find similar numbers of follicles ≥11mm. Automated-3D ultrasound has a higher predictive ability regarding the...
Article
Study question Is the probability of ongoing pregnancy higher in the fixed as compared to the flexible gonadotrophin releasing hormone (GnRH) antagonist protocol? Summary answer A higher probability of ongoing pregnancy is present by using the fixed as compared to the flexible GnRH antagonist protocol. What is known already Although fixed GnRH an...
Article
Study question Can metabolic profiling predict embryos at risk of chromosomal abnormalities and how are these reflected in the ultrustructure and cytoskeleton? Summary answer Different metabolic profiles are observed between normal and aneuploid/chaotic embryos which are linked to altered mitochondrial and other organelles’ structure/function and...
Article
Study question Are basal androgen concentrations associated with the number of follicles on the day of triggering final oocyte maturation in poor responders undergoing in vitro fertilization (IVF)? Summary answer A significant negative association is present between (dehydroepiandrosterone sulfate) DHEAS concentrations and the number of follicles...
Article
Study question Does testosterone pretreatment increase the probability of pregnancy in poor responders undergoing ovarian stimulation with gonadotrophin-releasing hormone (GnRH) analogues and gonadotrophins for in-vitro fertilization (IVF)? Summary answer Testosterone pretreatment increases clinical pregnancy and live birth rates in poor responder...
Article
Research question: Are there any differences in viability, spindle abnormalities and mitochondrial and other organelle structures amongst embryos biopsied on day 3 versus day 5 before and after vitrification? Design: A total of 240 day 3 biopsied embryos that developed to blastocysts but were rejected for transfer following preimplantation genet...
Chapter
Diabetes mellitus (DM) is a common disorder with substantial implications for disease burden and healthcare costs. It is characterized by increasing number of adolescents and young women with DM type 1 (T1DM), as well as DM type 2 (T2DM). Both T1DM and T2DM are significantly associated with female reproductive dysfunction, including an increased ri...
Article
Full-text available
(1) Background: Nowadays, pregnancy can be achieved by in vitro fertilisation (IVF) or by intracytoplasmic sperm injection (ICSI) for many infertile couples. However, implantation failure still remains a significant problem and it can be stressful for both patients and doctors. One of the key players for pregnancy achievement is the uterine environ...
Article
Full-text available
Background and Objectives: Ankylosing spondylitis (AS) is a condition that affects 0.1% to 0.5% of the adult population. The aim of this case report was to investigate the possible effects of the drugs taken for treatment of AS as well as mRNA vaccination for COVID-19 on semen quality by performing a highly detailed analysis. Materials and Methods:...
Article
Full-text available
Background An ectopic pregnancy, when the gestational sac is implanted outside of the uterine cavity, can be life-threatening. A cornual pregnancy is the most dangerous type of ectopic pregnancy since it can be misdiagnosed easily and has high mortality rate. It is diagnosed when the implantation site is at the junction between the fallopian tube a...
Article
Study question Are there any differences in implantation, cytoskeleton and ultrastructure of embryos biopsied on day5 vs day3 and how endometrial damage/inflammation may affect receptivity and implantation? Summary answer No differences are observed in implantation rates but vitrification following day5 biopsy led to more cytoskeletal/ultrastructu...
Article
Study question Does oral antioxidant supplementation improve sperm quality in infertile men with varicocele who have not undergone surgical repair? Summary answer Oral antioxidant supplementation improves sperm concentration and motility in infertile men with varicocele who have not undergone surgical repair. What is known already: Benefit from or...
Article
Study question Does radioactive iodine (RAI) treatment in premenopausal women with differentiated thyroid cancer (DTC) affects ovarian reserve, as evaluated by anti-Müllerian hormone (AMH), antral follicle count (AFC) and follicle-stimulating hormone (FSH)? Summary answer AMH concentrations decreased at three, six and 12 months following RAI treat...
Article
Full-text available
STUDY QUESTION Is it possible to define a set of performance indicators (PIs) for clinical work in ART, which can create competency profiles for clinicians and for specific clinical process steps? SUMMARY ANSWER The current paper recommends six PIs to be used for monitoring clinical work in ovarian stimulation for ART, embryo transfer, and pregnan...
Article
Background Polycystic ovary syndrome (PCOS) is a common cause of anovulatory infertility. According to the latest guidelines, letrozole should be considered as the first-line pharmacological treatment for women with WHO Group II anovulation or PCOS. However, the use of letrozole as an ovulation induction agent is not FDA or EMA approved, and its us...
Article
Research Question Do women receiving corifollitropin alfa with a gonadotropin-releasing hormone (GnRH) antagonist experience less emotional and/or physical exhaustion than women receiving standard of care gonadotropin with daily administration of GnRH agonist or antagonist? Design The CoRifollitropin EvAluation in PracTicE (CREATE) study was a pro...
Article
Aim : Radioactive iodine (RAI) is frequently used as adjuvant therapy in patients with differentiated thyroid cancer (DTC). However, its effect on ovarian reserve has not been fully elucidated, with studies yielding inconsistent results. The aim of this study was to systematically review and meta-analyze the best available evidence regarding the ef...
Article
Full-text available
First frozen embryo transfer (FET) in a freeze‐only cycle strategy is the preferred option in high responders, such as women with polycystic ovarian syndrome (PCOS), undergoing assisted reproductive technologies (ART), since it enhances live birth rate, while reducing the chance of moderate/severe ovarian hyperstimulation syndrome (OHSS) compared w...
Article
Full-text available
The aim of the present study was to test whether inhibition of ovarian primordial follicles and subsequent activation can be achieved by transient mTOR inhibition. In this preclinical investigation, forty-five female immature Wistar rats were randomized in 5 groups. The control group received subcutaneous saline injections. The other groups receive...
Article
Full-text available
[This corrects the article DOI: 10.1093/hropen/hoaa009.][This corrects the article DOI: 10.1093/hropen/hoaa009.].
Article
The association between the number of oocytes retrieved and fresh live birth rate (LBR) or cumulative LBR (CLBR) and whether there is an optimal number of oocytes retrieved where LBR or CLBR are maximised is one of the most relevant clinical questions, with conflicting results in available studies. A systematic review of all eligible studies (n=16)...
Article
Infertility may be an early indicator of later-life health risk development, such as cardiovascular disease (CVD), the leading cause of death globally. Various infertility-associated factors such as female age, polycystic ovarian syndrome, endometriosis and metabolic syndrome are also risk factors for CVD. Whether there is a real association betwee...
Article
Full-text available
PurposeThe objective of this systematic review and metaanalysis was to examine if the probability of pregnancy after ovarian stimulation for in vitro fertilization (IVF), using GnRH analogues and gonadotrophins is associated with serum estradiol level (Ε2) on the day of triggering final oocyte maturation with human chorionic gonadotrophin (hCG).Met...
Article
Ovarian tissue cryopreservation and transplantation (OTCT) is increasingly being used in young cancer patients for fertility restoration and prevention of premature ovarian insufficiency (POI) and has recently been advocated as a method to delay menopause. This is accomplished by cryopreserving ovarian tissue that is excised laparoscopically in thi...
Article
Full-text available
Study question: Can the grade of ascites, haematocrit (Ht), white blood cell (WBC) count and maximal ovarian diameter (MOD) measured on Day 3 be used to construct a decision-making algorithm for performing or cancelling embryo transfer in patients at high risk for severe ovarian hyperstimulation syndrome (OHSS) after an hCG trigger? Summary answe...
Article
Research Question Sex hormone-binding globulin (SHBG), androgen receptor (AR), luteinizing hormone beta polypeptide (LHB), progesterone receptor membrane component 1 (PGRMC1) and progesterone receptor membrane component 2 (PGRMC2) regulate follicle development and maturation. Their mRNA expression was assessed in peripheral blood mononuclear cells...
Article
Full-text available
Background: Women who achieve pregnancy by ART show an increased risk of obstetric and perinatal complications compared with those with spontaneous conception (SC). Objective and rationale: The purpose of this systematic review and meta-analysis was to synthesize the best available evidence regarding the association between ART and gestational d...
Article
Full-text available
Study question: What is the recommended management of ovarian stimulation, based on the best available evidence in the literature? Summary answer: The guideline development group formulated 84 recommendations answering 18 key questions on ovarian stimulation. What is known already: Ovarian stimulation for IVF/ICSI has been discussed briefly in...
Article
Background Endometriosis is one of the most common gynecological diseases of reproductive age, with a prevalence of 5–10% and grave consequences for quality of life and fertility. Vitamin D (vit D), a classic regulator of plasma calcium concentration and skeleton mineralization, is also an effective modulator of the immune system. Several studies s...
Article
Full-text available
Research question Is body-mass index (BMI) associated with oocyte maturation in women at high risk for developing severe ovarian hyperstimulation syndrome (OHSS) who are triggered with gonadotrophin releasing hormone (GnRH) agonist? Design Prospective observational cohort study. A total of 113 patients at high risk for severe OHSS (presence of at...
Article
Full-text available
Study question: Are oocyte maturation rates different among 0.1, 0.2 and 0.4 mg triptorelin used for triggering final oocyte maturation in patients at high risk for ovarian hyperstimulation syndrome (OHSS) undergoing ICSI? Summary answer: A dose of 0.1 mg triptorelin results in similar oocyte maturation rates compared to higher doses of 0.2 and...
Article
Full-text available
Several studies on semen physiology and sperm fertilizing capacity have shown a beneficial effect of antioxidants. Procyanidine is a natural antioxidant, more efficient compared with vitamin C and E, with many applications in the food, agriculture, pharmaceutical and cosmetic industry. Thus, we tested whether the addition of procyanidine to the sem...
Article
The aim of this study was to investigate the effects of zona drilling and biopsy on day 3 followed by vitrification on day 5 on the cytoskeleton and development of human embryos, by analysing survival rates and spindle and chromosome configurations by fluorescence and confocal laser scanning microscopy in human biopsied and non-biopsied embryos. In...
Article
Egg freezing was initially used as an intervention for iatrogenic ovarian insufficiency. Nowadays, it is not considered an experimental procedure, and it is increasingly popular among healthy women who wish to maintain their reproductive potential (fertility preservation). This review summarises the evidence on egg freezing technology, reproductive...
Article
Study question: Does the outcome of the comparison of live birth rates between the first frozen embryo transfer (ET) (in a freeze-only cycles strategy, i.e. frozen ET group) and a fresh embryo transfer (fresh ET group) differ considering the type of ovarian response? Summary answer: Α significantly higher probability of live birth is present in...
Chapter
How to Prepare the Egg and Embryo to Maximize IVF Success - edited by Gabor Kovacs January 2019
Article
Full-text available
Background Placental anomalies (placenta praevia, placental abruption, morbidly adherent placenta and cord insertion anomalies) are associated with maternal and fetal morbidity and mortality. It has been suggested these might be more prevalent in pregnancies after assisted reproduction technology (ART). Objectives To determine whether ART singleto...
Article
Full-text available
The use of testicular spermatozoa in men without azoospermia has been proposed as a means to increase the chances of pregnancy following assisted reproductive treatment. The purpose of this systematic review is to assess whether clinical outcomes are better when testicular rather than ejaculated spermatozoa are used for intracytoplasmic sperm injec...
Poster
Full-text available
Study question: Is luteal GnRH antagonist administration effective for the prevention and management of severe OHSS? Summary answer: Luteal GnRH-antagonist administration is not effective for the prevention of severe OHSS, while appropriate trials evaluating its use for outpatient management of OHSS are lacking. What is known already: Despite the i...
Article
Abbreviations: SER: smooth endoplasmic reticulum; ICSI: intracytoplasmic sperm injection; GnRH: gonadotrophin releasing hormone; CGH: comparative genomic hybridization; FISH: fluoresence in situ hybridization; FSH: follicle stimulating hormone; hCG: human chorionic gonadotrophin; OHSS: ovarian hyperstimulation syndrome; IVF: in vitro fertilization...
Article
Objective: To assess ultrasound and haematological changes in ovarian hyperstimulation syndrome (OHSS) during the early luteal phase in women at high risk for developing OHSS. Methods: This is a retrospective cohort study of 319 women undergoing in-vitro fertilisation (IVF), at high risk for OHSS following human chorionic gonadotrophin (hCG) tri...
Article
Full-text available
Purpose: The aim of this study is to determine whether blastocyst utilization rates are different after continuous culture in two different commercial single-step media. Methods: This is a paired randomized controlled trial with sibling oocytes conducted in infertility patients, aged ≤40 years with ≥10 oocytes retrieved assigned to blastocyst cu...
Chapter
Approximately five million children have been born worldwide as a result of assisted reproductive technology (ART). These techniques are now practised independently in most of the world's nations. Although the vast majority of ART parents and children are healthy following the procedures involved, there is an imperative to maintain a high standard...
Article
The aim of the present study was to compare blastocyst formation rates after embryo culture in a single medium (Global) as compared to sequential media (ISM1/BlastAssist). In this prospective trial with sibling oocytes, 542 metaphase II (MII) oocytes from 31 women were randomly and equally divided to be fertilized and cultured to the blastocyst sta...
Article
Vitrification is widely used to cryopreserve supernumerary embryos following in vitro fertilization (IVF). The mouse model was used to investigate the effects of vitrification on blastomere viability, using viability markers, and on the cytoskeleton, by analysing spindle/chromosome configurations, using confocal scanning microscopy. Ninety cleavage...
Article
STUDY QUESTION Are there any baseline predictors of progesterone elevation (PE) on the day of human chorionic gonadotrophin (hCG) which are not associated with the intensity of ovarian stimulation in women undergoing in vitro fertilization (IVF) using follicle stimulating hormone (FSH) and gonadotrophin-releasing hormone (GnRH) antagonists? SUMMAR...
Article
STUDY QUESTION Does pretreatment with transdermal testosterone increase the number of cumulus-oocyte complexes (COCs) retrieved by more than 1.5 in poor responders undergoing intracytoplasmic sperm injection (ICSI), using recombinant follicle stimulating hormone (FSH) and gonadotrophin releasing hormone agonists (GnRHa)?
Article
Without any doubt the regimen used to mature multiple capable oocytes for IVF impacts IVF outcomes. Studies have indicated that the inclusion of LH activity, adjuvant agents such as growth hormone (GH), and regimens providing for simultaneous action of both LH and FSH during final oocyte maturation may have beneficial effects on IVF outcomes. Becau...
Article
Clinical outcomes of IVF cycles using propofol or thiopental sodium as anaesthetic agents for oocyte retrieval were compared. The primary outcome measure was fertilization rate per patient. One hundred and eighty patients undergoing ovarian stimulation with gonadotrophins and gonadotrophin-releasing hormone antagonists for IVF were randomized to re...
Article
The aim of the present study was to assess changes in thyroid function and thyroid autoimmunity (TAI) throughout ovarian stimulation (OS) for intracytoplasmic sperm injection (ICSI) and the association of these changes with ICSI outcome. A flexible gonadotrophin-releasing hormone (GnRH) antagonist protocol was used in 42 women and their thyroid fun...