Stine Krog Frandsen

• PhD
• Researcher at Zealand University Hospital, Roskilde, Denmark

Background and Purpose: Calcium electroporation (CaEP) involves injecting calcium into tumour tissues and using electrical pulses to create membrane pores that induce cell death. This study assesses resultant immune responses and histopathological changes in patients with cutaneous metastases. Patients/Materials and Methods: The aimed cohort compri...

Background and study aims Colorectal cancer is one of the most common malignancies, with approximately 20 % of patients having metastatic disease. Local symptoms from the tumor remain a common issue and affect quality of life. Electroporation is a method to permeabilize cell membranes with high-voltage pulses, allowing increased passage of otherwis...

Calcium electroporation is a novel anticancer treatment where calcium is injected intratumorally followed by electroporation, which transiently permeabilizes the plasma membrane leading to high intracellular calcium and subsequent cell death. The treatment induces cell death in all tested cancer cell lines and tumor types across histologies but wit...

Introduction Skin malignancy is a distressing problem for many patients, and clinical management is challenging. This article describes the protocol for the Calcium Electroporation Response Study (CaEP-R) designed to investigate tumour response to calcium electroporation and is a descriptive guide to calcium electroporation treatment of malignant t...

Simple Summary Sarcoids are skin tumors on horses where better treatment options are needed. Calcium electroporation is a novel anti-cancer treatment where calcium is injected into the tumor, followed by brief electric pulses (electroporation) that transiently open the cell membrane and allow uptake of calcium. This, in turn, induces cell death. In...

Calcium electroporation is a potential novel anti-cancer treatment where high calcium concentrations are introduced into cells by electroporation, a method where short, high voltage pulses induce transient permeabilisation of the plasma membrane allowing passage of molecules into the cytosol. Calcium is a tightly regulated, ubiquitous second messen...

Electroporation is a method to permeabilise cell membranes. It can be categorised as 1) reversible electroporation in order to increase the uptake of therapeutic molecules such as chemotherapy, calcium, or 2) irreversible electroporation where the cell is permeabilised extensively, and both methods are used in cancer treatment. The treatment is bri...

[This corrects the article DOI: 10.18632/oncotarget.24352.].

Calcium electroporation is a novel anti-cancer treatment investigated in clinical trials. We explored cell sensitivity to calcium electroporation and electroporation with bleomycin, using viability assays at different time and temperature points, as well as heat calorimetry, lipidomics, and flow cytometry. Three cell lines: HT29 (colon cancer), MDA...

Calcium electroporation is a potential novel anticancer treatment, where high concentrations of calcium are introduced into the cell cytosol by electroporation. This is a method where short, high-voltage pulses induce a transient permeabilization of the cell membrane and thereby allow influx and efflux of ions and molecules. Electroporation is used...

Calcium electroporation (CaEP) is a novel anti-tumour treatment that induces cell death by internalization of large quantities of calcium. The anti-tumour effectiveness of CaEP has been demonstrated in vitro, in vivo, and in preliminary clinical trials; however, its effects on the vasculature have not been previously investigated. Using a dorsal wi...

Introduction: Electroporation mediated calcium ions is a new and very efficient anti-cancer therapy. Interestingly, an increase in intracellular calcium ions concentration induced by electroporation (EP) impacts differently on normal than malignant cells and causes disparate response of cellular components. Materials and methods: As a study model w...

Calcium electroporation (CaEP) describes the use of electric pulses (electroporation) to transiently permeabilize cells to allow supraphysiological doses of calcium to enter the cytosol. Calcium electroporation has successfully been investigated for treatment of cutaneous metastases in a clinical study. This preclinical study explores the possible...

Electroporation mediated calcium ions is a new and very efficient anti-cancer therapy. Interestingly, an increase in intracellular calcium ions concentration induced by electroporation (EP) impacts differently on normal and malignant cells. Cellular mechanisms responsible for calcium homeostasis such as calcium pumps and channels may play a crucial...

Electrochemotherapy is an anticancer treatment inducing uptake of chemotherapeutics by electroporation. This treatment is increasingly being used clinically and further investigations for improvement of the treatment and discovery of new drugs are ongoing. The most commonly used drugs in electrochemotherapy are bleomycin and cisplatin, but many oth...

Calcium electroporation may offer a simple general tool for anticancer therapy. Transient permeabilization of cancer cell membranes created by applying short, high-voltage pulses in tumors enables high calcium influxes that trigger cell death. In this study, we compared the relative sensitivity of different human tumor models and normal tissues to...

Background Calcium electroporation is a new experimental anti-cancer treatment where calcium is internalized into cells by application of short, high voltage pulses. Calcium electroporation has been shown to induce tumor necrosis associated with ATP depletion while the effect on normal fibroblasts was limited when investigated in a 3D in vitro sphe...

Viability and intracellular ATP level after calcium electroporation in vitro. Two human cancer cell lines (H69, small cell lung cancer and SW780, bladder cancer) were treated with increasing concentration of calcium with or without electroporation (EP). Viability was measured 1 day after treatment and intracellular ATP level was measured 1, 2, 4, a...

Rhabdomyosarcoma (RMS) is a malignant tumor derived from myoblasts that affect children and young adults and impacts also on the surrounding healthy muscle [1]. Increase in intracellular calcium level by electroporation (EP) may be a new approach to treat numerous types of cancer [2]. Calcium ions influence on cell proliferation as well as cell dea...

Electrochemotherapy is an anticancer treatment inducing uptake of chemotherapeutics by electroporation. This treatment is increasingly being used clinically and further investigations for improvement of the treatment and discovery of new drugs are ongoing. The most commonly used drugs in electrochemotherapy are bleomycin and cisplatin, but many oth...

Introduction Calcium is an important ubiquitous second messenger regarding the maintenance of cellular homeostasis, and is therefore tightly regulated. Calcium induces cell death when internalised into cancer cells after permeabilization of the cell membrane by electroporation. It is established that radiation causes damage to the lipids and protei...

Electroporation-based treatments and other therapies that permeabilize the plasma membrane have been shown to be more devastating to malignant cells than to normal cells. In this study, we asked if a difference in repair capacity could explain this observed difference in sensitivity. Membrane repair was investigated by disrupting the plasma membran...

Calcium electroporation describes the use of high voltage electric pulses to introduce supraphysiological calcium concentrations into cells. This promising method is currently in clinical trial as an anti-cancer treatment. One very important issue is the relation between tumor cell kill efficacy-and normal cell sensitivity.Using a 3D spheroid cell...

Electroporation in presence of calcium has been recently demonstrated to be capable of inducing cell death, suggesting that calcium might be used as an alternative to bleomycin in the palliative treatment of tumors via elec-trochemotherapy. In this work, SW780 bladder cancer cells were exposed to eight, 99 μs long, 1 Hz repetition rate pulses with...

Electroporation, a method for increasing the permeability of membranes to ions and small molecules, is used in the clinic with chemotherapeutic drugs for cancer treatment (electrochemotherapy). Electroporation with calcium causes ATP (adenosine triphosphate) depletion and cancer cell death and could be a novel cancer treatment. This study aims at u...

Multiple self-healing squamous epithelioma - Ferguson-Smith disease (MSSE) is an autosomal dominant inherited disease with multiple, recurrent, histologically malignant tumours that undergo spontaneous regression. The gene for MSSE has recently been identified as the transforming growth factor-beta receptor 1 (TGFBR1). Although rare, MSSE constitut...

Electroporation of cells with short, high-voltage pulses causes a transient permeabilization of cell membranes that permits passage of otherwise nonpermeating ions and molecules. In this study, we illustrate how electroporation with isotonic calcium can achieve highly effective cancer cell kill in vivo. Calcium electroporation elicited dramatic ant...