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Stine Krog Frandsen

Stine Krog Frandsen
Zealand University Hospital, Roskilde, Denmark · Department of Clinical Oncology

PhD

About

30
Publications
8,679
Reads
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698
Citations
Citations since 2016
23 Research Items
648 Citations
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2016201720182019202020212022020406080100120140
2016201720182019202020212022020406080100120140
2016201720182019202020212022020406080100120140
Additional affiliations
December 2017 - present
Zealand University Hospital, Roskilde, Denmark
Position
  • Human biologist, PhD
December 2016 - November 2017
University of Limoges
Position
  • PostDoc Position
January 2016 - September 2016
University of Copenhagen Herlev Hospital
Position
  • PostDoc Position
Education
August 2008 - January 2011
University of Copenhagen
Field of study
  • M.Sc. Human Biology
August 2007 - January 2008
Karlstads Universitet
Field of study
September 2005 - August 2008
University of Southern Denmark
Field of study
  • B.Sc. Biomedicine

Publications

Publications (30)
Chapter
Calcium electroporation is a novel anticancer treatment where calcium is injected intratumorally followed by electroporation, which transiently permeabilizes the plasma membrane leading to high intracellular calcium and subsequent cell death. The treatment induces cell death in all tested cancer cell lines and tumor types across histologies but wit...
Article
Full-text available
Introduction Skin malignancy is a distressing problem for many patients, and clinical management is challenging. This article describes the protocol for the Calcium Electroporation Response Study (CaEP-R) designed to investigate tumour response to calcium electroporation and is a descriptive guide to calcium electroporation treatment of malignant t...
Article
Full-text available
Sarcoids are common equine skin tumors where the risk of recurrence after treatment is high, and better treatment options are warranted. Calcium electroporation is a novel anti-cancer treatment where lethally high calcium concentrations are introduced into the cells by electroporation, a method where short high-voltage pulses induce transient perme...
Article
Full-text available
Calcium electroporation is a potential novel anti-cancer treatment where high calcium concentrations are introduced into cells by electroporation, a method where short, high voltage pulses induce transient permeabilisation of the plasma membrane allowing passage of molecules into the cytosol. Calcium is a tightly regulated, ubiquitous second messen...
Article
Full-text available
Electroporation is a method to permeabilise cell membranes. It can be categorised as 1) reversible electroporation in order to increase the uptake of therapeutic molecules such as chemotherapy, calcium, or 2) irreversible electroporation where the cell is permeabilised extensively, and both methods are used in cancer treatment. The treatment is bri...
Article
Full-text available
Calcium electroporation is a novel anti-cancer treatment investigated in clinical trials. We explored cell sensitivity to calcium electroporation and electroporation with bleomycin, using viability assays at different time and temperature points, as well as heat calorimetry, lipidomics, and flow cytometry. Three cell lines: HT29 (colon cancer), MDA...
Article
Full-text available
Calcium electroporation is a potential novel anticancer treatment, where high concentrations of calcium are introduced into the cell cytosol by electroporation. This is a method where short, high-voltage pulses induce a transient permeabilization of the cell membrane and thereby allow influx and efflux of ions and molecules. Electroporation is used...
Article
Full-text available
Calcium electroporation (CaEP) is a novel anti-tumour treatment that induces cell death by internalization of large quantities of calcium. The anti-tumour effectiveness of CaEP has been demonstrated in vitro, in vivo, and in preliminary clinical trials; however, its effects on the vasculature have not been previously investigated. Using a dorsal wi...
Conference Paper
Introduction: Electroporation mediated calcium ions is a new and very efficient anti-cancer therapy. Interestingly, an increase in intracellular calcium ions concentration induced by electroporation (EP) impacts differently on normal than malignant cells and causes disparate response of cellular components. Materials and methods: As a study model w...
Article
Full-text available
Calcium electroporation (CaEP) describes the use of electric pulses (electroporation) to transiently permeabilize cells to allow supraphysiological doses of calcium to enter the cytosol. Calcium electroporation has successfully been investigated for treatment of cutaneous metastases in a clinical study. This preclinical study explores the possible...
Conference Paper
Electroporation mediated calcium ions is a new and very efficient anti-cancer therapy. Interestingly, an increase in intracellular calcium ions concentration induced by electroporation (EP) impacts differently on normal and malignant cells. Cellular mechanisms responsible for calcium homeostasis such as calcium pumps and channels may play a crucial...
Chapter
Electrochemotherapy is an anticancer treatment inducing uptake of chemotherapeutics by electroporation. This treatment is increasingly being used clinically and further investigations for improvement of the treatment and discovery of new drugs are ongoing. The most commonly used drugs in electrochemotherapy are bleomycin and cisplatin, but many oth...
Article
Full-text available
Calcium electroporation may offer a simple general tool for anticancer therapy. Transient permeabilization of cancer cell membranes created by applying short, high-voltage pulses in tumors enables high calcium influxes that trigger cell death. In this study, we compared the relative sensitivity of different human tumor models and normal tissues to...
Article
Full-text available
Calcium electroporation is a new experimental anti-cancer treatment where calcium is internalized into cells by application of short, high voltage pulses. Calcium electroporation has been shown to induce tumor necrosis associated with ATP depletion while the effect on normal fibroblasts was limited when investigated in a 3D in vitro spheroid model....
Data
Viability and intracellular ATP level after calcium electroporation in vitro. Two human cancer cell lines (H69, small cell lung cancer and SW780, bladder cancer) were treated with increasing concentration of calcium with or without electroporation (EP). Viability was measured 1 day after treatment and intracellular ATP level was measured 1, 2, 4, a...
Conference Paper
Rhabdomyosarcoma (RMS) is a malignant tumor derived from myoblasts that affect children and young adults and impacts also on the surrounding healthy muscle [1]. Increase in intracellular calcium level by electroporation (EP) may be a new approach to treat numerous types of cancer [2]. Calcium ions influence on cell proliferation as well as cell dea...
Chapter
Electrochemotherapy is an anticancer treatment inducing uptake of chemotherapeutics by electroporation. This treatment is increasingly being used clinically and further investigations for improvement of the treatment and discovery of new drugs are ongoing. The most commonly used drugs in electrochemotherapy are bleomycin and cisplatin, but many oth...
Conference Paper
Introduction Calcium is an important ubiquitous second messenger regarding the maintenance of cellular homeostasis, and is therefore tightly regulated. Calcium induces cell death when internalised into cancer cells after permeabilization of the cell membrane by electroporation. It is established that radiation causes damage to the lipids and protei...
Article
Full-text available
Electroporation-based treatments and other therapies that permeabilize the plasma membrane have been shown to be more devastating to malignant cells than to normal cells. In this study, we asked if a difference in repair capacity could explain this observed difference in sensitivity. Membrane repair was investigated by disrupting the plasma membran...
Article
Full-text available
Background Calcium electroporation describes the use of high voltage electric pulses to introduce supraphysiological calcium concentrations into cells. This promising method is currently in clinical trial as an anti-cancer treatment. One very important issue is the relation between tumor cell kill efficacy–and normal cell sensitivity. Methods Usin...
Conference Paper
Electroporation in presence of calcium has been recently demonstrated to be capable of inducing cell death, suggesting that calcium might be used as an alternative to bleomycin in the palliative treatment of tumors via elec-trochemotherapy. In this work, SW780 bladder cancer cells were exposed to eight, 99 μs long, 1 Hz repetition rate pulses with...
Article
Full-text available
Electroporation, a method for increasing the permeability of membranes to ions and small molecules, is used in the clinic with chemotherapeutic drugs for cancer treatment (electrochemotherapy). Electroporation with calcium causes ATP (adenosine triphosphate) depletion and cancer cell death and could be a novel cancer treatment. This study aims at u...
Article
Multiple self-healing squamous epithelioma - Ferguson-Smith disease (MSSE) is an autosomal dominant inherited disease with multiple, recurrent, histologically malignant tumours that undergo spontaneous regression. The gene for MSSE has recently been identified as the transforming growth factor-beta receptor 1 (TGFBR1). Although rare, MSSE constitut...
Article
Full-text available
Electroporation of cells with short, high-voltage pulses causes a transient permeabilization of cell membranes that permits passage of otherwise nonpermeating ions and molecules. In this study, we illustrate how electroporation with isotonic calcium can achieve highly effective cancer cell kill in vivo. Calcium electroporation elicited dramatic ant...

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Projects

Projects (2)
Project
To collect preclinical and clinical data about calcium electroporation, a novel cancer treatment utilizing cell permeabilisation by electroporation to introduce supraphysiological doses of calcium into cancer cells.
Project
The aim of the enhanced perioperative oncology (EPEONC) consortium is to integrate principles of optimized surgical, anesthesiological and oncological treatments in order to improve the short- and long-term outcomes of patients undergoing cancer surgery. The proposed project includes proof of concept clinical studies determining the most effective combination for reducing post-operative stress and maximizing the immune system stimulation. In parallel, in vitro and in vivo experiments as well as epidemiological studies will be carried out, to gain insight into cellular mechanisms and global co-factors affecting these processes.