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Introduction
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September 2005 - September 2008
Publications
Publications (24)
Summary
- Poly-tert-butylphenol disulfide (trade name Vultac© TB7) was able to covalently modify EPO.
- This is the first time, at least to our knowledge, that such a modification could be confirmed experimentally.
- The disulfide bonds of EPO are the vulnerable sites for Vultac© attack.
- In the presence of human serum albumin (HSA), Vultac© react...
Screening assays for protein reactive E/L's. Application of GSH-trapping with or without electrochemical activation. Application of GSSG-screening to detect E/L's, which can modify disulfide bridges. Case study: EPREX could be covalently modified by the rubber leachable Vultac.
Extractable and leachable studies performed on the immediate container closure system for biopharmaceutical drug products are well established in the pharmaceutical industry with best practice guidances and guidelines which outline what is expected and acceptable. Studies adhering to these guidances address the potential of an extractable or leacha...
Abiotic stresses in general and extracellular acidity in particular disturb and limit nitrogen-fixing symbioses between rhizobia and their host legumes. Except for valuable molecular-biological studies on different rhizobia, no consolidated models have been formulated to describe the central physiologic changes that occur in acid-stressed bacteria....
The γ-proteobacterium Xanthomonas campestris pv. campestris (Xcc) B100 synthesizes the polysaccharide xanthan, a commercially important viscosifier. Since the complete genome of Xcc B100 is available, systems biology tools were applied to obtain a deeper understanding of the metabolism involved in xanthan biosynthesis. A large-scale metabolic netwo...
Pharmaceuticals based on proteins (biologicals), such as monoclonal antibodies (mAb), attain more and more relevance since they were established as potent drugs in anticancer therapy or for the treatment of autoimmune based diseases. Due to their high efficiency it is essential to have accurate and precise methods for protein quantitation and the d...
Table S1. X. dendrorhous proteins identified by MALDI-TOF MS. This table lists all MS-identified proteins that were separated by 2D electrophoresis.
Figure S1. GC-MS analysis of sterols from wild-type and cyp61 X. dendrorhous mutant strain. GC profiles of sterols (peaks Nº 1, 2 and 3) from UCD 67–385 (panel A) and 385-cyp61(−/−) (panel B) strains. Sterols structures were identified according to their retention times and mass spectra (NIST Standard Reference Database). Panels C, D and E show the...
Carbon source assimilation by yeast isolates obtained in this work. Determinations were performed using the API ID 32C gallery (bioMérieux, Lyon, France) according to manufacturer′s instructions. Gal, D-galactose; Sac, D-sucrose; Nag, N-acetyl-glucosamine; Lat, lactic acid; Ara, L-arabinose; Cel, D-cellobiose; Raf, D-raffinose; Mal, maltose; Tre, D...
Fig. S2. Differential abundance proteins from X. dendrorhous. Shown are a representative proteins spots during the growth.
The yeast Xanthophyllomyces dendrorhous is used for the microbiological production of the antioxidant carotenoid astaxanthin. In this study, we established an optimal protocol for protein extraction and performed the first proteomic analysis of the strain ATCC 24230. Protein profiles before and during the induction of carotenogenesis were determine...
Burkholderia cenocepacia produces a diffusible fatty acid signal molecule, cis-2-dodecenoic acid (BDSF), that has been implicated in interspecies and interkingdom communication. Here, we show that BDSF
also acts as an intraspecies signal in B. cenocepacia to control factors contributing to virulence of this major opportunistic pathogen.
Xanthomonas campestris pathovar campestris (Xcc) is a plant pathogenic bacterium and as such has to adapt to a variety of environments. During the course of disease, Xcc colonizes the surface of its host, infects the xylem in the early stages, and develops a fully saprophytic life-style, aided by secreted degradative enzymes, in the late stages. To...
Outer membrane vesicles (OMVs) are released from the outer membrane of many Gram-negative bacteria. These extracellular compartments are known to transport compounds involved in cell-cell signalling as well as virulence associated proteins, e.g. the cytolysine from enterotoxic E. coli.
We have demonstrated that Xanthomonas campestris pv. campestris...
Interspecies signalling through the action of diffusible signal molecules can influence the behaviour of organisms growing in polymicrobial communities. Stenotrophomonas maltophilia and Pseudomonas aeruginosa occur ubiquitously in the environment and can be found together in diverse niches including the rhizosphere of plants and the cystic fibrosis...
The complete genome sequence of the Xanthomonas campestris pv. campestris strain B100 was established. It consisted of a chromosome of 5,079,003 bp, with 4471 protein-coding genes and 62 RNA genes. Comparative genomics showed that the genes required for the synthesis of xanthan and xanthan precursors were highly conserved among three sequenced X. c...
Three of the most abundant proteins (OmpW, MopB and SodM) of the extracellular proteome of Xanthomonas campestris pv. campestris were analysed in a luminol-based oxidative burst assay to identify novel pathogen-associated molecular patterns (PAMP). Tobacco cell suspension cultures were used as a model system to monitor elicitor induced plant defenc...
The extracellular proteome of Xanthomonas campestris pv. campestris (Xcc) cultivated in minimal medium was isolated from the cell-free culture supernatant and separated by two-dimensional gel electrophoresis. This technique resolved 97 clearly visible protein spots, which were excised, digested with trypsin and identified on the basis of their pept...
The chemically-coded affinity tag (CCAT) method combines standard electrophoresis protocols with MALDI-TOF-MS analysis to identify and quantify protein abundances in complex samples in one step. This method is designed to fit into the workflow of SDS-PAGE or two-dimensional electrophoresis (2-DE) only requiring basic proteome laboratory equipment....
