Stephen TC Wong

Stephen TC Wong
Houston Methodist Hospital | HOUSTONMETHODIST · Systems Medicine and Bioengineering

PhD

About

647
Publications
100,591
Reads
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19,081
Citations
Citations since 2017
66 Research Items
9748 Citations
201720182019202020212022202305001,0001,500
201720182019202020212022202305001,0001,500
201720182019202020212022202305001,0001,500
201720182019202020212022202305001,0001,500
Additional affiliations
January 2010 - March 2017
Houston Methodist Hospital
Position
  • Chair
May 2007 - present
Weill Cornell Medical College
Position
  • Professor
May 2007 - February 2015
Houston Methodist Hospital
Position
  • Chief of Medical Physics, Chief Research Information Officer, and Chairman of Department of Systems Medicine and Bioengineering

Publications

Publications (647)
Article
Full-text available
The way in which cells adopt different morphologies is not fully understood. Cell shape could be a continuous variable or restricted to a set of discrete forms. We developed quantitative methods to describe cell shape and show that Drosophila haemocytes in culture are a heterogeneous mixture of five discrete morphologies. In an RNAi screen of genes...
Article
Full-text available
Recent advances in automated high-resolution fluorescence microscopy and robotic handling have made the systematic and cost effective study of diverse morphological changes within a large population of cells possible under a variety of perturbations, e.g., drugs, compounds, metal catalysts, RNA interference (RNAi). Cell population-based studies dev...
Article
Purpose: Human cytomegalovirus (HCMV) is a common neurotropic herpes virus although its DNA was confirmed highly prevalent in various peripheral cancers. HCMV is also a major cause of neuropathology in immune-compromised patients. Since HCMV reactivation frequently causes encephalopathy during chemotherapy or radiation therapy in brain tumor patien...
Article
NOS2 and COX2 expression is significantly correlated with poor prognosis in ER- breast cancer. Multiple pro-oncogenic pathways involving metastasis, proliferation, angiogenesis, immunosuppression, and chemoresistance are stimulated in a synergistic manner by NOS2 and COX2, resulting in much more aggressive cancers. In this study, our single-cell RN...
Article
Background Breast cancer is the most frequently diagnosed cancer in women in developed countries with increased incidence in women at high risk such as those with strong family history, BRCA mutations, atypical hyperplasia etc. Chemoprevention with drugs like tamoxifen and aromatase inhibitors come with challenges of intolerance and limited efficac...
Article
Background Brain metastases is a fatal consequence of advanced breast cancer with a poor prognosis. Both tumor intrinsic molecular underpinning of the primary tumor and microenvironmental brain niche factors have been reported to allow invading cancer cells to colonize and outgrow into the brain. A systemic investigation of these tumor intrinsic an...
Article
Brain metastases are diagnosed in 20-40% of all cancer patients. Despite the advancements in neurosurgery, radiotherapy, and chemotherapy, one-year survival of metastatic brain tumor patients still within a single digit such that brain metastases constitute about 20% of annual cancer deaths. These facts clearly indicate a critical need for better u...
Article
Translating images generated by label-free microscopy imaging, such as Coherent Anti-Stokes Raman Scattering (CARS), into more familiar clinical presentations of histopathological images will help the adoption of real-time, spectrally resolved label-free imaging in clinical diagnosis. Generative adversarial networks (GAN) have made great progress i...
Article
Brain metastasis (BrM) occurs in 25-40% of TNBC metastatic patients while one-year survival remains a single digit. In contrast to other primary subtypes, TNBC usually spreads to the brain rapidly at early stages or even before the diagnosis of primary cancer. The shorter development time of BrM in TNBC and shorter survival time after BrM diagnosis...
Article
Objectives Based on differences in chemosensitivity and clinical outcomes, we hypothesize that clear cell ovarian carcinoma (CCCO) may display a unique tumor immune microenvironment compared to high grade serous ovarian carcinoma (HGSOC). In addition, micro-satellite instability (MSI) and ARID1DA mutations are more common in clear cell ovarian carc...
Article
Label-free high-resolution molecular and cellular imaging strategies for intraoperative use are much needed, but not yet available. To fill this void, we developed an artificial intelligence-augmented molecular vibrational imaging method that integrates label-free and subcellular-resolution coherent anti-stokes Raman scattering (CARS) imaging with...
Article
Full-text available
Patients with inflammatory bowel disease often present to the emergency department due to the chronic relapsing nature of the disease. Previous studies have shown younger patients to have an increased frequency of emergency department visits, resulting in repeated exposure to imaging studies and steroids, both of which are associated with risks. We...
Article
Full-text available
Computer-aided diagnosis (CAD) systems must constantly cope with the perpetual changes in data distribution caused by different sensing technologies, imaging protocols, and patient populations. Adapting these systems to new domains often requires significant amounts of labeled data for re-training. This process is labor-intensive and time-consuming...
Preprint
Full-text available
Although stromal and immune cells in the tumor microenvironment have been shown to directly affect tumor growth and chemoresistance, how the interactions among stromal and immune cells and their spatially resolved cell heterogeneity would influence ovarian patients’ survival remains largely unknown. To fill this gap, we developed a new imageomics m...
Conference Paper
The role of immune checkpoint inhibitors (ICPIs) in ovarian cancer continues to be refined. The presence of intratumoral T cells in ovarian tumors correlates with improved survival and progression, suggesting that immune modulation, such as by ICPIs, may be beneficial. However, a better understanding of the immune effects following ICPI use, partic...
Article
Full-text available
Advanced ovarian cancer usually spreads to the omentum. However, the omental cell-derived molecular determinants modulating its progression have not been thoroughly characterized. Here, we show that circulating ITLN1 has prognostic significance in patients with advanced ovarian cancer. Further studies demonstrate that ITLN1 suppresses lactotransfer...
Article
Full-text available
Global pandemics call for large and diverse healthcare data to study various risk factors, treatment options, and disease progression patterns. Despite the enormous efforts of many large data consortium initiatives, scientific community still lacks a secure and privacy-preserving infrastructure to support auditable data sharing and facilitate autom...
Article
Full-text available
Background: Hyperamyloidosis in the brain is known as the earliest neuropathological change and a unique etiological factor in Alzheimer's disease (AD), while progressive neurodegeneration in certain vulnerable brain regions forms the basis of clinical syndromes. It is not clear how early hyperamyloidosis is implicated in progressive neurodegenera...
Poster
Full-text available
Participants Tiancheng He, Houston, TX (Presenter) Nothing to Disclose Mamta Puppala, Houston, TX (Abstract Co-Author) Nothing to Disclose Chika Ezeana, Houston, TX (Abstract Co-Author) Nothing to Disclose Rebecca Danforth, Houston, TX (Abstract Co-Author) Nothing to Disclose Zheng Yin, Houston, TX (Abstract Co-Author) Nothing to Disclose Shenyi Ch...
Article
Full-text available
Patient falls during hospitalization can lead to severe injuries and remain one of the most vexing patient-safety problems facing hospitals. They lead to increased medical care costs, lengthened hospital stays, more litigation, and even death. Existing methods and technology to address this problem mostly focus on stratifying inpatients at risk, wi...
Article
Full-text available
Background: We present data on a cohort of patients diagnosed with sepsis over a 10-year period comparing outcomes in solid organ transplant (SOT) and non-solid organ transplant (non-SOT) recipients. Methods: This is a retrospective single-center study of patients with diagnosis of sepsis from 1/1/06 to 6/30/16. Cases and controls were matched b...
Article
Full-text available
Metastases are responsible for the majority of breast cancer–associated deaths. The contribution of epithelial-to-mesenchymal transition (EMT) in the establishment of metastases is still controversial. To obtain in vivo evidence of EMT in metastasis, we established an EMT lineage tracing (Tri-PyMT) model, in which tumor cells undergoing EMT would i...
Article
Full-text available
Motivation: Drug combinations that simultaneously suppress multiple cancer driver signaling pathways increase therapeutic options and may reduce drug resistance. We have developed a computational systems biology tool, DrugComboExplorer, to identify driver signaling pathways and predict synergistic drug combinations by integrating the knowledge emb...
Article
INTRODUCTION Although biologics and immunomodulators are commonly prescribed as maintenance therapy in patients with inflammatory bowel disease (IBD), steroids continue to be prescribed during acute flares and in refractory disease. There are significant risks associated with steroids and strategies are needed to decrease their utilization and opti...
Article
INTRODUCTION Patients with inflammatory bowel disease (IBD) frequently have CT scans performed during emergency department (ER) visits and hospitalizations. Previous studies found that the younger a patient is when a CT scan is performed, the higher the risk of malignancy over a lifetime. Younger IBD patients have also been shown to have an increas...
Article
Ewing sarcoma (EwS) is an aggressive pediatric tumor treated with intensive cytotoxic chemotherapies. Overall survival for metastatic or relapsed disease is only 20-30%. Metformin has long been an attractive therapeutic option for EwS, but hypoxia limits its efficacy. Through a systematic integration of drug combination screening, bioinformatics an...
Article
Purpose: Recent studies demonstrate the role of the tumor microenvironment in tumor progression. However, strategies used to overcome the malignant phenotypes of cancer cells modulated by the microenvironment have not been thoroughly explored. In this study, we evaluated the therapeutic efficacy of a newly developed mAb targeting microfibril-assoc...
Conference Paper
Efforts to develop STAT3 inhibitors have focused on its SH2 domain, starting with short phosphotyrosylated peptides with STAT3 binding motifs, e.g. pY905LPQTV within gp130. Despite binding to STAT3 with high affinity, issues regarding stability, bioavailability, and membrane permeability of these peptides, as well as peptidomimetics such as CJ-887,...
Conference Paper
Ovarian cancer, especially high-grade serous ovarian cancer (HGSOC), is the deadliest gynecological cancer with 50-70% 5-year mortality rates. Every year more than 22,000 new cases of ovarian cancer and 15,000 deaths are anticipated within the United States only. Recent clinical and experimental evidence indicates that tumor-associated macrophages...
Conference Paper
Efforts to develop STAT3 inhibitors have focused on its SH2 domain, starting with short phosphotyrosylated peptides with STAT3 binding motifs, e.g. pY905LPQTV within gp130. Despite binding to STAT3 with high affinity, issues regarding stability, bioavailability, and membrane permeability of these peptides, as well as peptidomimetics such as CJ-887,...
Article
Full-text available
Most cancer deaths are due to metastasis, and almost all cancers have their preferential metastatic organs, known as “organotropism metastasis”. Epithelial-mesenchymal plasticity has been described as heterogeneous and dynamic cellular differentiation states, supported by emerging experimental evidence from both molecular and morphological levels....
Article
Full-text available
Purpose: The Breast Imaging Reporting and Data System (BI-RADS) lexicon was developed to standardize mammographic reporting to assess cancer risk and facilitate the decision to biopsy. Because of substantial interobserver variability in the application of the BI-RADS lexicon, the decision to biopsy varies greatly and results in overdiagnosis and e...
Article
Objective: Components of the adipose tissue (AT) extracellular matrix (ECM) are recently discovered contributors to obesity-related cardiometabolic disease. We identified increased adipocyte expression of ECM-related clusterin (apolipoprotein J) in obese versus lean women by microarray. Our objective was to determine 1) whether subcutaneous AT adi...
Article
Full-text available
This study aims to discover genes with prognostic potential for glioblastoma (GBM) patients’ survival in a patient group that has gone through standard of care treatments including surgeries and chemotherapies, using tumor gene expression at initial diagnosis before treatment. The Cancer Genome Atlas (TCGA) GBM gene expression data are used as inpu...
Article
Full-text available
Ras GTPases are powerful drivers for tumorigenesis, but directly targeting Ras for treating cancer remains challenging. The growth and transforming activity of the aggressive basal-like breast cancer (BLBC) are driven by N-Ras. To target N-Ras in BLBC, this study screened existing pharmacologically active compounds for the new ability to induce N-R...
Article
The fate of disseminated tumor cells is largely determined by microenvironment (ME) niche. The osteogenic niche promotes cancer cell proliferation and bone metastasis progression. We investigated the underlying mechanisms using pre-clinical models and analyses of clinical data. We discovered that the osteogenic niche serves as a calcium (Ca) reserv...
Article
There are distinct cell subpopulations in normal epithelial tissue, including stem cells, progenitor cells, and more differentiated cells, all of which have been extensively studied for their susceptibility to tumorigenesis. However, normal cells usually have to progress through a precancerous lesion state before becoming a full-blown tumor. Precan...
Article
Full-text available
Purpose: Only 34% of breast cancer survivors engage in the recommended level of physical activity because of a lack of accountability and motivation. Methodist Hospital Cancer Health Application (MOCHA) is a smartphone tool created specifically for self-reinforcement for patients with cancer through the daily accounting of activity and nutrition a...
Article
Medulloblastoma (MB) is the most common malignant brain tumor of childhood. Although outcomes have improved in recent decades, new treatments are still needed to improve survival and reduce treatment-related complications. The MB subtypes groups 3 and 4 represent a particular challenge due to their intragroup heterogeneity, which limits the options...
Article
Full-text available
Success of immune checkpoint inhibitors in advanced non-small-cell lung cancer (NSCLC) has invigorated their use in the neoadjuvant setting for early-stage disease. However, the cellular and molecular mechanisms of the early immune responses to therapy remain poorly understood. Through an integrated analysis of early-stage NSCLC patients and a Kras...
Article
Ovarian cancer is the most lethal gynecologic malignancy in the US. While research efforts have been dedicated to identify disease related genes and mutations in cancer cells, the stroma-tumor interactions in the tumor microenvironment and their roles in disease progression is relatively unexplored. The identification of stroma-tumor crosstalk netw...
Article
Full-text available
Background: Bulk tumor tissue samples are used for generating gene expression profiles in most research studies, making it difficult to decipher the stroma-cancer crosstalk networks. In the present study, we describe the use of microdissected transcriptome profiles for the identification of cancer-stroma crosstalk networks with prognostic value, w...
Article
Purpose: Recent studies indicated that dysregulation of noncoding RNAs (ncRNA) such as miRNAs is involved in pathogenesis of various human cancers. However, the molecular mechanisms underlying miR-34a are not fully understood in triple-negative breast cancer (TNBC). Experimental Design: We performed in vitro functional assays on TNBC cell lines to...
Article
Immunotherapies targeting T-cell functionality, such as anti-PD-1, have been shown to have efficacy in numerous cancers, including non-small cell lung cancer (NSCLC). However, treatment with anti-PD-1 induces responses in only a subset of patients. This raises the possibility that other effectors of T-cell dysfunction remain to be elucidated. To ev...
Article
Full-text available
Chemotherapy-induced diarrhea (CID), with clinical high incidence, adversely affects the efficacy of cancer treatment and patients' quality of life. Our study demonstrates that the citrus flavonoid hesperetin (Hst) has a superior potential as a new agent to prevent and alleviate CID. In the animal model for irinotecan (CPT-11) induced CID, Hst coul...
Article
The molecular mechanism by which cancer-associated fibroblasts (CAFs) confer chemoresistance in ovarian cancer is poorly understood. The purpose of the present study was to evaluate the roles of CAFs in modulating tumor vasculature, chemoresistance, and disease progression. Here, we found that CAFs upregulated the lipoma-preferred partner (LPP) gen...
Data
Document S1. Supplemental Experimental Procedures, Figures S1–S7, and Tables S1–S3
Article
Full-text available
Here, we show that HEMATOLOGICAL AND NEUROLOGICAL EXPRESSED 1-LIKE (HN1L) is a targetable breast cancer stem cell (BCSC) gene that is altered in 25% of whole breast cancer and significantly correlated with shorter overall or relapse-free survival in triple-negative breast cancer (TNBC) patients. HN1L silencing reduced the population of BCSCs, inhib...
Article
Full-text available
Lung cancer is the most prevalent type of cancer and the leading cause of cancer-related deaths worldwide. Coherent anti-Stokes Raman scattering (CARS) is capable of providing cellular-level images and resolving pathologically related features on human lung tissues. However, conventional means of analyzing CARS images requires extensive image proce...
Article
Purpose: To compare costs of care and quality outcomes between teaching and non-teaching hospitalist services, while testing the assumption resident-driven care is more expensive. Method: Records of inpatients with the top 20 Medicare Severity Diagnosis-Related Groups (MS-DRGs) admitted to the University Teaching Service (UTS) and non-teaching h...
Article
Background: The natural history of de novo donor specific antibodies (dnDSA) after lung transplantation is not well-described. We sought to determine the incidence and risk factors associated with dnDSA and compare outcomes between recipients with transient (or isolated) versus persistent dnDSA after transplantation. Methods: A single center rev...
Article
Full-text available
The majority of breast cancer models for drug discovery are based on orthotopic or subcutaneous tumours. Therapeutic responses of metastases, especially microscopic metastases, are likely to differ from these tumours due to distinct cancer-microenvironment crosstalk in distant organs. Here, to recapitulate such differences, we established an ex viv...
Data
Supplementary Figures and Supplementary Tables
Article
Background This study aims to investigate the prognostic role of complete metabolic response (CMR) on interim 18F-fluorodeoxyglucose positron emission tomography/computed tomography (PET/CT) in patients with breast cancer (BC) receiving neoadjuvant chemotherapy (NAC) according to tumor subtypes and PET timing. Patients and Methods Eighty-six conse...
Article
Full-text available
Subarachnoid hemorrhage (SAH) in 95% of cases results in long-term disabilities due to brain damage, pathogenesis of which remains uncertain. Hindrance of cerebrospinal fluid (CSF) circulation along glymphatic pathways is a possible mechanism interrupting drainage of damaging substances from subarachnoid space and parenchyma. We explored changes in...
Article
Aim: To determine the prevalence of in-hospital non-steroidal anti-inflammatory drug (NSAID) exposure and associated outcomes in patients admitted with a primary diagnosis of heart failure. Methods: We performed a propensity-matched cohort analysis of patients admitted to Houston Methodist Hospital System with a primary diagnosis of heart failur...
Conference Paper
Resting-state functional magnetic resonance imaging (rs-fMRI) provides key neural imaging characteristics for quantitative assessment and better understanding of the mechanisms of attention deficit hyperactivity disorder (ADHD). Recent multivariate analysis studies showed that functional connectivity (FC) could be used to classify ADHD from normal...
Article
Full-text available
Dynamic susceptibility contrast (DSC) perfusion-weighted imaging (PWI) is widely used in clinical settings for the radiological diagnosis of brain tumor. The signal change in brain tissue in gradient echo-based DSC PWI is much higher than in spin echo-based DSC PWI. Due to its exquisite sensitivity, gradient echo-based sequence is the preferred met...
Article
Background: A key challenge to mining electronic health records for mammography research is the preponderance of unstructured narrative text, which strikingly limits usable output. The imaging characteristics of breast cancer subtypes have been described previously, but without standardization of parameters for data mining. Methods: The authors...
Article
Full-text available
In coherent anti-Stokes Raman scattering (CARS) and second harmonic generation (SHG) imaging, backward and forward generated photons exhibit different image patterns and thus capture salient intrinsic information of tissues from different perspectives. However, they are often mixed in collection using traditional image acquisition methods and thus...