Stephen Feist

Stephen Feist
Centre for Environment, Fisheries and Aquaculture Science | CEFAS · Division of Aquatic Health and Hygiene

PhD

About

249
Publications
79,400
Reads
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9,798
Citations
Additional affiliations
January 1979 - present
Centre for Environment, Fisheries and Aquaculture Science
Position
  • Principle pathologist, Scientific advisor

Publications

Publications (249)
Article
Full-text available
Agapollen is a traditional heliothermic marine oyster lagoon in western Norway, representing the northernmost site of any Marteilia sp. detected in Europe. The semi-closed lagoon is a unique site to study the life cycle and development of Marteilia pararefringens in naïve mussels. Two baskets with uninfected mussels were deployed in the lagoon outl...
Article
Full-text available
The velvet swimming crab Necora puber has been fished in Ireland since the early 1980s and contributes significant income to smaller fishing vessels. From 2016 onwards, reduced landings have been reported. We undertook a full pathological investigation of crabs from fishing grounds at 3 sites on the west (Galway), southwest (Castletownbere) and eas...
Article
Full-text available
An unusual condition affecting market size rainbow trout was investigated. This condition was prevalent for several years at low levels but affected a large proportion of stock during 2018 and 2019. Chronic fibrosis affecting cranial tissues and the jaw was observed in samples collected in 2018. A larger sampling was then conducted in 2019 to inves...
Preprint
Full-text available
This study provides a morphological, ultrastructural, and phylogenetic characterization of a novel micro-eukaryotic parasite (2.3-2.6 µm) infecting genera Echinogammarus and Orchestia . Longitudinal studies across two years revealed that infection prevalence peaked in late April and May, reaching 64% in Echinogammarus sp. and 15% in Orchestia sp.,...
Article
Full-text available
Intracellular microcolonies of bacteria (IMC), in some cases developing large extracellular cysts (bacterial aggregates), infecting primarily gill and digestive gland, have been historically reported in a wide diversity of economically important mollusk species worldwide, sometimes associated with severe lesions and mass mortality events. As an eff...
Article
Full-text available
In late 2018, unusual patterns of very high mortality (>50% production) were reported in intensive tilapia cage culture systems across Lake Volta in Ghana. Samples of fish and fry were collected and analysed from two affected farms between October 2018 and February 2019. Affected fish showed darkening, erratic swimming and abdominal distension with...
Article
Full-text available
Aquaculture is predicted to supply the majority of aquatic dietary protein by 2050. For aquaculture to deliver significantly enhanced volumes of food in a sustainable manner, appropriate account needs to be taken of its impacts on environmental integrity, farmed organism health and welfare, and human health. Here, we explore increased aquaculture p...
Article
Full-text available
This study provides a morphological and phylogenetic characterization of two novel species of the order Haplosporida (Haplosporidium carcini n. sp., and H. cranc n. sp.) infecting the common shore crab Carcinus maenas collected at one location in Swansea Bay, South Wales, UK. Both parasites were observed in the haemolymph, gills and hepatopancreas....
Article
Full-text available
This is the first record of a fish nidovirus isolated from a consignment of goldfish at the United Kingdom (UK) border. The full-length viral genome was 25,985 nt, sharing a 97.9% nucleotide identity with the Chinook salmon bafinivirus (CSBV) NIDO with two deletions of 537 and 480 nt on the ORF Ia protein. To assess the potential impact on UK fish...
Article
Hepatocellular fibrillar inclusions (HFI) are an unusual pathology of unknown aetiology affecting European flounder (Platichthys flesus), particularly from estuaries historically impacted by pollution. This study demonstrated that the HFI prevalence range was 6–77% at several UK estuaries, with Spearman rank correlation analysis showing a correlati...
Article
Red mark syndrome (RMS) is a skin condition in Rainbow trout Oncorhynchus mykiss that has been reported worldwide but was first seen in the United Kingdom (UK) in 2003. The current study was conducted to examine if there was an association between a Midichloria-like organism (MLO) and RMS using a statistically appropriate sample set, whilst determi...
Chapter
Full-text available
This chapter provides an introduction to important marine pathogens, providing an overview of the diversity of pathogen types and how they affect different hosts in the marine environment. The chapter focuses on wild and cultured species and highlights that single infections are relatively rare, with co- and secondary infections being commonplace....
Article
Full-text available
Wild-caught ballan wrasse Labrus bergylta are translocated en masse from the British south-west coast to Scotland for use as cleaner fish to tackle Atlantic salmon Salmo salar sea lice infestations; however, very little is known about the background health status of this species. This is the first health assessment of wild ballan wrasse from the Br...
Article
Full-text available
This study provides morphological, ultrastructural and phylogenetic characterization of 2 novel species of Haplosporidia (Haplosporidium echinogammari n. sp. and H. orchestiae n. sp.) infecting amphipods of the genera Echinogammarus and Orchestia collected in southwestern England. Both parasites infect the connective tissues associated with the dig...
Article
The growth of aquaculture over the past 50 years has been accompanied by the emergence of aquatic animal diseases, many of which have spread to become pandemic in countries or continents. An analysis of 400 emerging disease events in aquatic animals that were logged by the Centre for Environment, Fisheries and Aquaculture Science between 2002 and 2...
Preprint
Full-text available
Outbreaks of disease with unprecedented levels of mortalities have been affecting tilapia production in Lake Volta, Ghana since October 2018. In this study, we identified infectious spleen kidney necrosis virus (ISKNV) as the likely cause of massive mortalities in two farms investigated. ISKNV is a virus from the genus Megalocytivirus family Iridov...
Article
Full-text available
The Lyme Bay marine protected area (MPA) hosts a valuable population of king scallop Pecten maximus L. Recently, an Endozoicomonas-like organism (ELO), infecting host gill epithelial tissue, was associated with king scallop mass mortality events within the Lyme Bay MPA. Currently, very little is known about its transmission and survival outside the...
Technical Report
Full-text available
Pathology and diseases of fish and shellfish in marine environments of the ICES region
Article
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Marteilia refringens causes marteiliosis in oysters, mussels and other bivalve molluscs. This parasite previously comprised two species, M. refringens and Marteilia maurini , which were synonymized in 2007 and subsequently referred to as M. refringens ‘O-type’ and ‘M-type’. O-type has caused mass mortalities of the flat oyster Ostrea edulis . We us...
Article
Full-text available
Puffy skin disease (PSD) is an emerging skin condition which affects rainbow trout, Oncorhynchus mykiss (Walbaum). The transmission pattern of PSD suggests an infectious aetiology, however, the actual causative infectious agent(s) remain(s) unknown. In the present study, the rainbow trout epidermal immune response to PSD was characterised. Skin sam...
Article
Full-text available
A parasite exhibiting Oomycete-like morphology and pathogenesis was isolated from discoloured eggs of the European lobster (Homarus gammarus) and later found in gill tissues of adults. Group-specific Oomycete primers were designed to amplify the 18S ribosomal small subunit (SSU), which initially identified the organism as the same as the 'Haliphtho...
Article
Full-text available
The edible mussel Mytilus edulis is a major aquaculture commodity in Europe, with 168000 t produced in 2015. A number of abundant, well characterised parasites of the species are known, though none are considered to cause significant mortality. Haplosporida (Rhizaria, Endomyxa) is an order of protistan parasites of aquatic invertebrates, the best s...
Article
Full-text available
Within aquatic habitats, the hyper-abundant Order Crustacea appear to be the predominant host group for members of the Phylum Microsporidia. The musculature, a common site of infection, provides access to biochemical (carbohydrate-rich) and physiological (mitochondria-rich) conditions conducive to prolific parasite replication and maturation. The s...
Article
Full-text available
One of the fastest growing fisheries in the UK is the king scallop (Pecten maximus L.), also currently rated as the second most valuable fishery. Mass mortality events in scallops have been reported worldwide, often with the causative agent(s) remaining uncharacterized. In May 2013 and 2014, two mass mortality events affecting king scallops were re...
Conference Paper
Full-text available
Goals of INHAND Non-rodent Fish Working Group (NRFWG) Heike Schmidt-Posthaus1, James Baily2, Wes Baumgartner3, Thomas Braunbeck4, Stephen Feist5, Satoshi Furukawa6, Yuuki Katou7, Christine Rühl-Fehlert8, Stephen Schmidt9, Helmut Segner1, Jan Spitsbergen10, Jeff Wolf11 1Centre for Fish and Wildlife Health, University of Bern, Bern, Switzerland 2Ch...
Conference Paper
This poster reports efforts of an international working group of pathologists to establish standardized diagnostic criteria and standardized nomenclature for lesions of various organ systems of finfish.
Article
Full-text available
Marine environmental monitoring is undertaken to provide evidence that environmental management targets are being met. Moreover, monitoring also provides context to marine science and over the last century has allowed development of a critical scientific understanding of the marine environment and the impacts that humans are having on it. The seas...
Article
Bacteria from the genus Flavobacteriaceae often show low susceptibility to antibiotics. With the exception of two Chryseobacterium spp. isolates that were positive for the florfenicol resistance gene floR, no clinical resistance genes were identified by microarray in 36 Flavobacteriaceae isolates from salmonid fish that could grow in ≥ 4 mg/L florf...
Article
Full-text available
The Paramyxida, closely related to haplosporidians, paradinids, and mikrocytids, is an obscure order of parasitic protists within the class Ascetosporea. All characterized ascetosporeans are parasites of invertebrate hosts, including molluscs, crustaceans and polychaetes. Representatives of the genus Marteilia are the best studied paramyxids, large...
Article
Full-text available
The transmission of puffy skin disease (PSD) to rainbow trout Oncorhynchus mykiss Walbaum was tested in the laboratory by conducting co-habitation challenges with puffy skin (PS)-affected fish (Trojans) collected from the field. Two separate challenges were conducted using Trojans sourced from two different sites and diploid (first trial) or triplo...
Data
Histopathological changes observed in gills of rainbow trout during the second cohabitation trial with puffy skin affected fish. (DOCX)
Data
Number of reads before and after quality trimming and after removal of reads representing rainbow trout sequences. (DOCX)
Data
Summary statistics for the de novo assembled transcripts. (DOCX)
Data
Taxonomic classification of eukaryotic rRNA sequences in the affected and non-affected rainbow trout skin samples. (DOCX)
Data
Taxonomic classification of bacterial rRNA sequences in the affected and non-affected rainbow trout skin samples. (DOCX)
Data
Lists of all annotated sequences (and their corresponding sequence information) for each sample. (XLSX)
Data
Histopathological changes observed in kidney of rainbow trout during the second cohabitation trial with puffy skin affected fish. (DOCX)
Data
Histopathological changes observed in spleen of rainbow trout during the second cohabitation trial with puffy skin affected fish. (DOCX)
Data
Histopathological changes observed in heart of rainbow trout during the second cohabitation trial with puffy skin affected fish. (DOCX)
Article
Full-text available
Paramyxida is an order of rhizarian protists that parasitise marine molluscs, annelids and crustaceans. They include notifiable pathogens (Marteilia spp.) of bivalves and other taxa of economic significance for shellfish production. The diversity of paramyxids is poorly known, particularly outside of commercially important hosts, and their phylogen...
Article
Full-text available
Chemokine modulation in response to pathogens still needs to be fully characterised in fish, in view of the recently described novel chemokines present. This paper reports the first comparative study of CXC chemokine genes transcription in salmonids (brown trout), with a particular focus on the fish specific CXC chemokines (CXCL_F). Adopting new pr...
Article
Ranaviruses can be transmitted by contaminated water and sediment but must retain infectivity for a sufficient period to reach and infect a susceptible host. To determine the risk a virus represents once it enters the environment, its persistence in that environment must be determined. We evaluated the survival of frog virus 3 (FV3) in water and se...
Article
Full-text available
Comparative pathology as a scientific discipline studies animal diseases in relation to their aetiology, pathogenesis and prognosis. Among the main aspects of this discipline, regressive changes, host defense responses with pathological implications and progressive changes, represent the majority of the possible responses of cells and tissues to pa...
Article
Full-text available
Two myxozoan species were observed in the kidney of topsmelt, Atherinops affinis, during a survey of parasites of estuarine fishes in the Carpinteria Salt Marsh Reserve, California. Fish collected on three dates in 2012 and 2013 were sectioned and examined histologically. Large extrasporogonic stages occurred in the renal interstitium of several fi...
Article
Full-text available
journal published by Elsevier. The attached copy is furnished to the author for internal non-commercial research and education use, including for instruction at the authors institution and sharing with colleagues. Other uses, including reproduction and distribution, or selling or licensing copies, or posting to personal, institutional or third part...
Chapter
Full-text available
This chapter describes general cellular processes associated with myxozoan development and includes the first review of motility at the cellular level. We consider modes of cell proliferation by endogeny and division by mitosis and meiosis. We also describe cellular processes associated with sporogony, including the formation of plasmodia and spore...
Article
Full-text available
The deep-sea environment is a sink for a wide variety of contaminants including heavy metals and organic compounds of anthropogenic origin. Life history traits of many deep-water fish species including longevity and high trophic position may predispose them to contaminant exposure and subsequent induction of pathological changes, including tumour f...
Article
Full-text available
In the Mediterranean Sea, anglerfish Lophius budegassa and Lophius piscatorius support an important fishery, and landings have increased in recent years. These species are infected by the microsporean Spraguea lophii, a parasite that infects their nervous system giving rise to multiple cysts. Due to the high value of these fish and conspicuous natu...