Stéphane Brézillon

Stéphane Brézillon
Université de Reims Champagne-Ardenne | URCA · Département de Biologie, Biochimie, Terre et Environnement

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88
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5,917
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Citations since 2016
39 Research Items
2962 Citations
20162017201820192020202120220100200300400500
20162017201820192020202120220100200300400500
20162017201820192020202120220100200300400500
20162017201820192020202120220100200300400500

Publications

Publications (88)
Article
Au cours du choc septique, le remplissage vasculaire corrige l’hypovolémie liée à la fuite capillaire, responsable de l’état de choc et de l’apparition progressive d’œdèmes généralisés. La fuite capillaire a longtemps été considérée comme principalement due à des modifications de l’endothélium vasculaire et du glycocalyx. Or, le rôle central du tis...
Article
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The hair renewal involves changes in the morphology of the hair follicle and its micro-vascularization. In alopecia, the hair cycle is accelerated, resulting in the formation of thinner and shorter hair. In addition, alopecia is associated with a decrease in the micro-vascularization of the hair follicles. In this study, the role of glypicans (GPCs...
Article
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Ovarian cancer remains one of the most fatal cancers due to a lack of robust screening methods of detection at early stages. Extracellular matrix (ECM) mediates interactions between cancer cells and their microenvironment via specific molecules. Lumican, a small leucine-rich proteoglycan (SLRP), maintains ECM integrity and inhibits both melanoma pr...
Article
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Lumican, a small leucine-rich proteoglycan (SLRP) of the extracellular matrix (ECM), displays anti-tumor properties through its direct interaction with MMP-14. Lumican-derived peptides, such as lumcorin (17 amino acids) or L9M (10 amino acids), are able to inhibit the proteolytic activity of MMP-14 and melanoma progression. This work aimed to visua...
Article
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It was reported that lumican inhibits the activity of metalloproteinase MMP-14 and melanoma cell migration in vitro and in vivo. Moreover, Snail triggers epithelial-to-mesenchymal transition and the metastatic potential of cancer cells. Therefore, the aim of this study was to examine the effect of lumican on Mock and Snail overexpressing melanoma B...
Article
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The expression of glypicans in different hair follicle (HF) compartments and their potential roles during hair shaft growth are still poorly understood. Heparan sulfate proteoglycan (HSPG) distribution in HFs is classically investigated by conventional histology, biochemical analysis, and immunohistochemistry. In this report, a novel approach is pr...
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Glycosaminoglycans (GAGs)/proteoglycans (PGs) play a pivotal role in the metastasis of inflammatory breast cancer (IBC). They represent biomarkers and targets in diagnosis and treatment of different cancers including breast cancer. Thus, GAGs/PGs could represent potential prognostic/diagnostic biomarkers for IBC. In the present study, non-IBC MDA-M...
Article
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The transcription factor Snail triggers epithelial-to-mesenchymal transition (EMT), endowing cancer cells with invasive properties during tumor progression. Extracellular vesicles (EVs) released from cancer cells at various stages of cancer progression are known to influence the tumor pre-metastatic niche and metastatic potential. The aim of this s...
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Heparan sulfate proteoglycans (HSPGs) act as signaling coreceptors by interactions of their sulfated glycosaminoglycan chains with numerous signaling molecules. In breast cancer, the function of heparan sulfate 2‐O‐sulfotransferase (HS2ST1 ), the enzyme mediating 2‐O‐sulfation of HS, is largely unknown. Hence, a comparative study on the functional...
Article
Full-text available
Melanoma is the most aggressive type of cutaneous malignancies. In addition to its role as a regulator of extracellular matrix (ECM) integrity, lumican, a small leucine-rich proteoglycan, also exhibits anti-tumor properties in melanoma. This work focuses on the use of infrared spectral imaging (IRSI) and histopathology (IRSH) to study the effect of...
Article
Full-text available
Proteoglycans (PG) play an important role in maintaining the extracellular matrix (ECM) integrity. Lumican, a small leucine rich PG, is one such actor capable of regulating such properties. In this study, the integrity of the dermis of lumican-deleted Lum–/– vs. wild-type mice was investigated by conventional histology and by infrared spectral hist...
Article
Full-text available
The tumor microenvironment (TME) is composed of various cell types embedded in an altered extracellular matrix (ECM). ECM not only serves as a support for tumor cell but also regulates cell–cell or cell–matrix cross-talks. Alterations in ECM may be induced by hypoxia and acidosis, by oxygen free radicals generated by infiltrating inflammatory cells...
Article
The small leucine‐rich proteoglycan (SLRP) lumican regulates estrogen receptors (ERs)‐associated functional properties of breast cancer cells, expression of matrix macromolecules and epithelial‐to‐mesenchymal transition. However, it is not known whether the ER‐dependent lumican effects on breast cancer cells are related to the expression of integri...
Article
A great hallmark of breast cancer is the absence or presence of estrogen receptors ERα and ERβ, with a dominant role in cell proliferation, differentiation and cancer progression. Both receptors are related with Epithelial-to-Mesenchymal Transition (EMT) since there is a relation between ERs and extracellular matrix (ECM) macromolecules expression,...
Article
Background: Glypicans (GPCs) are heparan sulfate cell membrane proteoglycans containing glycosylphosphatidylinositol (GPI) anchor. They play important role in cell behavior by activating/presenting numerous growth factors and cytokines. Objectives: The expression of GPCs was investigated in primary culture of skin keratinocytes sampled from heal...
Article
Mammalian glycosaminoglycans are linear complex polysaccharides comprising heparan sulfate, heparin, dermatan sulfate, chondroitin sulfate, keratan sulfate and hyaluronic acid. They bind to numerous proteins and these interactions mediate their biological activities. GAG–protein interaction data reported in the literature are curated mostly in Matr...
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Inflammatory breast cancer (IBC) has a poor prognosis because of the lack of specific biomarkers and its late diagnosis. An accurate and rapid diagnosis implemented early enough can significantly improve the disease outcome. Vibrational spectroscopy has proven to be useful for cell and tissue characterization based on the intrinsic molecular inform...
Article
The extracellular matrix (ECM) constitutes a highly dynamic three-dimensional structural network comprised of macromolecules, such as proteoglycans/glycosaminoglycans (PGs/GAGs), collagens, laminins, fibronectin, elastin, other glycoproteins and proteinases. In recent years, the field of PGs has expanded rapidly. Due to their high structural comple...
Article
Wound healing, a complex physiological process, is responsible for tissue repair after exposure to destructive stimuli, without resulting in complete functional regeneration. Injuries can be stromal or epithelial, and most cases of wound repair have been studied in the skin and cornea. Lumican, a small leucine-rich proteoglycan, is expressed in the...
Article
Full-text available
Lumican is a small leucine-rich proteoglycan (SLRP) being known as a key regulator of collagen fibrillogenesis. However, little attention has been given so far in studying its influence on tumor-associated matrix architecture. Here, we investigate the role of host lumican on tumor matrix organization as well as on disease progression considering an...
Article
Full-text available
Small leucine-rich proteoglycans (SLRPs) are important regulators of extracellular matrix assembly and cell signaling. They are a family of proteoglycans that are present in extracellular matrix and that share in common multiple repeats of a leucine-rich structural motif. SLRPs have been identified as inhibitors of cancer progression by affecting M...
Chapter
Proteoglycans are ubiquitous macromolecules of extracellular matrix, cell surface, and some intracellular granules. They are composed of a glycoprotein core to which one or several glycosaminoglycan chains are attached by covalent linkage. More than 40 different genes encode proteoglycans. Most of them are expressed in skin cells. However, only som...
Article
Full-text available
Glycosaminoglycans (GAGs) are natural, linear and negatively charged heteropolysaccharides which are incident in every mammalian tissue. They consist of repeating disaccharide units, which are composed of either sulfated or non-sulfated monosaccharides. Depending on tissue types, GAGs exhibit structural heterogeneity such as the position and degree...
Article
Full-text available
Lumican is a small leucine-rich proteoglycan that has been shown to contribute in several physiological processes, but also to exert anticancer activity. On the other hand, it has been recently shown that knockdown of the estrogen receptor α (ERα) in low invasive MCF-7 (ERα+) breast cancer cells and the suppression of ERβ in highly aggressive MDA-M...
Article
Full-text available
Spectroscopic markers characteristic of reference glycosaminoglycan molecules were identified previously based on their vibrational signatures. Infrared spectral signatures of glycosaminoglycans in fixed cells were also recently demonstrated but probing live cells still remains challenging. Raman microspectroscopy is potentially interesting to perf...
Article
Full-text available
Lumican is a small leucine-rich proteoglycan that has been shown to contribute in several physiological processes, but also to exert anticancer activity. On the other hand, it has been recently shown that knockdown of the estrogen receptor α (ERα) in low invasive MCF-7 (ERα+) breast cancer cells and the suppression of ERβ in highly aggressive MDA-M...
Article
Different mono-xylosides and their corresponding xylobiosides obtained by a chemo-enzymatic approach featuring various substituents attached to a triazole ring were probed as priming agents for glycosaminoglycan (GAG) biosynthesis in the xylosyltransferase-deficient pgsA-745 Chinese hamster ovary cell line. Xylosides containing a hydrophobic aglyco...
Article
Full-text available
The objective of the present study was to investigate the effects of different levels of copper (as supplemental copper-methionine) on ascites incidence and matrix metalloproteinase-2 (MMP-2) changes in the lungs of cold-stressed broilers. For this purpose, 480 1-day-old Ross 308 broiler chickens were randomly assigned to six treatments. Treatments...
Article
Full-text available
Lumican, a small leucine rich proteoglycan, inhibits MMP-14 activity and melanoma cell migration in vitro and in vivo. Snail triggers epithelial-mesenchymal transitions endowing epithelial cells with migratory and invasive properties during tumor progression. The aim of this work was to investigate lumican effects on MMP-14 activity and migration o...
Data
Effect of lumican on MMP-2 and MMP-9 activities in mock B16F1 cells. Cell-conditioned concentrated media were analyzed on SDS-polyacrylamide gels containing 1 mg/ml gelatin. (TIFF)
Data
Cell migration of Snail overexpressing B16F1 cells (clone 21). Cell migration of Snail21-B16F1 in presence or absence of lumican (100 nM) after 24h and 48h; n = 2, *p < 0.05. (TIFF)
Data
MMP-14 activity in mock and Snail overexpressing B16F1 clones. The measurements were performed as described in Materials and Methods. Mean±SEM, n = 3 experiments performed in duplicate. (TIFF)
Data
MMP-14 expression in mock and Snail-B16F1 cells (clone 19). Mean±SEM, n = 3 experiments performed in duplicate. n.s = non-significant. (TIFF)
Data
List of primers used for quantitative real time PCR reaction. SNAI1: snail family zinc finger 1; MMP-14: matrix metalloproteinase-14; EF1a: elongation factor 1 alpha 1; GAPDH: glyceraldehyde-3-phosphate dehydrogenase. (DOCX)
Chapter
Proteoglycans are ubiquitous macromolecules of extracellular matrix, cell surface, and some intracellular granules. They are composed of a glycoprotein core to which one or several glycosaminoglycan chains are attached by covalent linkage. More than 40 different genes encode proteoglycans. Most of them are expressed in skin cells. However, only som...
Article
We previously showed that lumican regulates MMP-14 expression. The aim of this study was to compare the effect of lumican and decorin on MMP-14 activity. In contrast to decorin, the glycosylated form of lumican was able to significantly decrease MMP-14 activity in B16F1 melanoma cells. Our results suggest that a direct interaction occurs between lu...
Article
Full-text available
We recently identified vibrational spectroscopic markers characteristic of standard glycosaminoglycan (GAG) molecules. The aims of the present work were to further this investigation to more complex biological systems and to characterize, via their spectral profiles, cell types with different capacities for GAG synthesis. After recording spectral i...
Article
Full-text available
Lumican, a small leucine-rich proteoglycan of the extracellular matrix, presents potent anti-tumor properties. Previous works from our group showed that lumican inhibited melanoma cell migration and tumor growth in vitro and in vivo. Melanoma cells adhered to lumican, resulting in a remodeling of their actin cytoskeleton and preventing their migrat...
Article
Lumican is a member of the small leucine-rich proteoglycan family. It is present in numerous extracellular matrices of different tissues such as muscle, cartilage and cornea. In skin, lumican is present as a glycoprotein. It plays a critical role in collagen fibrillogenesis as evidenced by knocking out of its gene in mouse. A direct link between lu...
Article
Full-text available
Tumor microenvironment is a complex system composed of a largely altered Extracellular Matrix (ECM) with different cell types that determine tumor progression. Upon the influence of hypoxia, tumor cells secrete cytokines that activate stromal cells to produce proteases and angiogenic factors. The proteases degrade the stromal ECM and participate in...
Article
Full-text available
Increasing number of evidence shows that soluble factors and extracellular matrix (ECM) components provide an optimal microenvironment controlling human bone marrow mesenchymal stem cell (MSC) functions. Successful in vivo administration of stem cells lies in their ability to migrate through ECM barriers and to differentiate along tissue-specific l...
Data
Expression of FAK-pY397 and total FAK in MSC and EPC. Cells were grown to confluence on 6-well plate. Expression of FAK-pY397 and total FAK in MSC and EPC was analyzed by Western immunoblotting after 15 min incubation without or with 100 nM of lumican. The levels of FAK-pY397 and total FAK were quantified by densitometric analysis and the FAK-pY397...
Data
Effect of lumican on the proliferation and the apoptosis of MSC and EPC. (A): Proliferation assay of MSC and EPC cultured on plastic or lumican-coated (30 µg/cm2) 6-well plates for 7 or 21 days, respectively. The results were reported as mean values (O.D. 560) ±S.D. (n = 3). (B): Cells were grown to 80% confluence for 24 h on coverslips pre-coated...
Data
Effect of blocking antibodies raised against human β1 and α2 integrin subunits on MSC migration in presence or absence of lumican. Blocking antibodies (10 µg/ml) anti-human α2 (MAB 1950) and β1 (MAB 1951) integrin subunits were incubated with MSC during 24 h in vitro wound healing assays in presence or absence of 100 nM lumican. The cell migration...
Data
Actin cytoskeleton distribution in MSC and EPC seeded on non-coated glass coverslips or coated with type I collagen, fibronectin, and lumican. MSC (a–d) and EPC (e–h) were grown to 80% confluence for 24 h on glass coverslips (a, e) pre-coated with 30 µg/cm2 type I collagen (b, f), 10 µg/ml fibronectin (c, g), or 30 µg/cm2 lumican (d, h). The distri...
Data
Gallardin® effect on MSC and EPC tube-like formation. Tube formation on Matrigel® (a-d) in control (a, c) or 10−9 M Gallardin® supplemented medium (b, d) was observed 24 hours after MSC (a, b) and EPC (c, d) seeding. Representative photographs are presented on the left panel. The semi-quantitative evaluation of the tube network from ten randomly se...
Data
List of primers used for quantitative real time PCR reaction. (DOC)
Data
Top 12 genes down-regulated and up-regulated in EPC versus MSC. (DOC)
Data
Lumican effect on dermal fibroblast tube-like formation. Tube formation on Matrigel® in control (a) or 100 nM lumican supplemented medium (b) was observed 24 hours after dermal fibroblast seeding (a, b). Representative photographs are presented on the left panel. The semi-quantitative evaluation of the tube network from ten randomly selected fields...
Article
Full-text available
NC1 domains from α1, α2, α3 and α6(IV) collagen chains were shown to exert anti-tumor or anti-angiogenic activities, whereas the NC1 domain of the α4(IV) chain did not show such activities so far. We demonstrate in the present paper that the NC1 α4(IV) domain exerts a potent anti-tumor activity both in vitro and in an experimental human melanoma mo...
Data
NC1[α4(IV)1-229] overexpression does not induce UACC-903 cell apoptosis. Cell nuclei were stained with Hoechst 33342 (1 μg/mL). Nuclear morphology was visualized with an inverted fluorescence microscope. No nuclear fragmentation was observed. Scale bar = 10 µm. (TIF)
Data
Effect of NC1 [α4(IV)1-229] overexpression on proteolytic cascades. Mock or NC1 α4(IV)-overexpressing cells were incubated for 48 h without FBS. A) Plasmin generated activity was measured in conditioned media using the H-D-Val-Leu-Lys-pNA (S-2251) peptide as a substrate and absorbance was recorded at 405 nm. NS: Non significant, ***: p<0.001. B) MM...
Article
Vibrational spectroscopies (VS), INFRARED SPECTROSCOPY and RAMAN SPECTROSCOPY, are well-established techniques for exploring the chemical composition of samples. VS are based on the molecular vibrations and give a spectral signature also called "molecular fingerprint" characteristic of the studied material. Recent advances in these techniques have...