Stephan Pollmann

Stephan Pollmann
Universidad Politécnica de Madrid | UPM · Centre for Plant Biotechnology and Genomics

Professor

About

158
Publications
34,144
Reads
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Introduction
Main objective of our lab is to investigate how plant hormone crosstalk provides plasticity to plant growth and development, and how plant hormones help to integrate and translate external cues into appropriate responses. To reach this goal we apply molecular, genetic, bioinformatics, as well as advanced transcriptomics and metabolomics approaches in order to obtain a comprehensive and elaborate picture of those interactions in selected developmental processes.
Additional affiliations
January 2019 - October 2021
Universidad Politécnica de Madrid
Position
  • Professor (Full)
Description
  • Regulatory hormonal networks
June 2013 - July 2013
University of Western Australia
Position
  • Professor
Description
  • Introduction into Next Generation Sequencing data analysis
September 2010 - December 2018
Universidad Politécnica de Madrid
Position
  • Professor
Description
  • Regulatory hormonal networks
Education
July 2004 - February 2009
Ruhr-Universität Bochum
Field of study
  • Botany
January 2000 - November 2002
Ruhr-Universität Bochum
Field of study
  • Molecular Plant Physiology
September 1993 - December 1999
Ruhr-Universität Bochum
Field of study
  • Biology

Publications

Publications (158)
Article
Full-text available
The accumulation of the auxin precursor indole-3-acetamide (IAM) in the ami1 mutant has recently been reported to reduce plant growth and to trigger abiotic stress responses in Arabidopsis thaliana. The observed response includes the induction of abscisic acid (ABA) biosynthesis through the promotion of NCED3 expression. The mechanism by which plan...
Preprint
Full-text available
Calcium (Ca ) is an important second messenger in plants. The activation of Ca signaling cascades is critical in the activation of adaptive processes in response to perceived environmental stimuli, including biotic stresses. The colonization of roots by the plant growth promoting endophyte Serendipita indica involves the increase of cytosolic Ca le...
Article
Expansins are proteins involved in cell wall metabolism that play an important role in plant growth, development, fruit ripening and abiotic stress tolerance. In the present study, we analyzed putative expansins that respond to drought stress. Five expansin genes were identified in cDNA libraries isolated from Colobanthus quitensis gown either with...
Article
Full-text available
The diversification of land plants largely relies on their ability to cope with constant environmental fluctuations, which negatively impact their reproductive fitness and trigger adaptive responses to biotic and abiotic stresses. In this limiting landscape, cumulative research attention has centred on deepening the roles of major phytohormones, mo...
Chapter
The potato is among the most important food crops in the world and of incalculable value for global food security. In 2012, the crop area for potato in Northern and Western Europe reached almost 1 million ha and a production of over 37 million tons with an average yield between 18 and 45 tons/ha. However, current potato production is put in jeopard...
Preprint
Full-text available
The indole-3-pyruvic acid pathway is the major route for auxin biosynthesis in higher plants. Tryptophan aminotransferases (TAA1/TAR) and members of the YUCCA family of flavin-containing monooxygenases catalyze the conversion of L-tryptophan via indole-3-pyruvic acid into indole-3-acetic acid (IAA). It has been described that locally produced jasmo...
Preprint
Full-text available
The evolutionary success of land plants largely relies on their ability to cope with constant environmental fluctuations, which negatively impact their reproductive fitness and trigger adaptational responses to biotic and abiotic stresses. In this challenging scenario, comprehensive research efforts so far aimed at depicting the roles of well-known...
Preprint
Full-text available
The major auxin, indole-3-acetic acid (IAA), is associated with a plethora of growth and developmental processes including embryo development, expansion growth, cambial activity, and the induction of lateral root growth. Accumulation of the auxin precursor indole-3-acetamide (IAM) induces stress related processes by stimulating abscisic acid (ABA)...
Preprint
Full-text available
The evolutionary success of plants relies to a large extent on their extraordinary ability to adapt to changes in their environment. These adaptations require that plants balance their growth with their stress responses. Plant hormones are crucial mediators orchestrating the underlying adaptive processes. However, whether and how the growth-related...
Article
Full-text available
While activation of resistance (R) proteins has been intensively studied, the downstream signaling mechanisms leading to restriction of pathogen remain mostly unknown. We studied the immunity network response conditioned by the potato Ny‐1 gene against potato virus Y. We analyzed the processes in the cell death zone and surrounding tissue on the bi...
Article
Full-text available
The global climate change is arguably one of the biggest threats of our times that already led to a wide range of impacts on the environment, economy, and society. Due to past emissions and the inertia of the climate system, global climate change is estimated to continue for decades even though anthropogenic greenhouse gas emissions could be stoppe...
Preprint
Full-text available
While activation of resistance (R) proteins has been intensively studied, the mechanisms acting downstream of R protein activation remain elusive. We studied effector-triggered immunity (ETI) conditioned by the potato Ny-1 gene against potato virus Y. Transcriptomic, ultrastructural and biochemical analyses of four consecutive tissue sections in an...
Article
Full-text available
The channeling of metabolites is an essential step of metabolic regulation in all living organisms. Multifunctional enzymes with defined domains for metabolite compartmentalization are rare, but in many cases, larger assemblies forming multimeric protein complexes operate in defined metabolic shunts. In Arabidopsis thaliana, a multimeric complex wa...
Article
Plants respond to the loss of vertical growth re-orientating their affected organs. In trees, this phenomenon has received the scientific attention due to its importance for the forestry industry. Nowadays it is accepted that auxin distribution is involved in the modulation of the tilting response, but how this distribution is controlled is not ful...
Article
Full-text available
Oxygenated membrane fatty acid derivatives dubbed oxylipins play important roles in the plant's defense against biotic and abiotic cues. Plants challenged by insect pests, for example, synthesize a blend of different defense compounds that, amongst others, comprise volatile aldehydes and jasmonic acid (JA). Because all oxylipins are derived from th...
Article
Full-text available
Auxin regulates diverse aspects of flower development in plants, such as differentiation of the apical meristem, elongation of the stamen, and maturation of anthers and pollen. It is known that auxin accumulates in pollen, but little information regarding the biological relevance of auxin in this tissue at different times of development is availabl...
Chapter
Over recent years, metabolomics found its way into daily laboratory routine as an additional 'omics' platform technology, playing an increasingly important role in modern plant sciences. It is often complementing other approaches such as transcriptomics or proteomics in systems biology experiments. Representing the end products of biochemical proce...
Article
Antarctic environments are amongst the most stressful habitats for life on Earth, with high intensities of solar UV-B radiation reaching the land surface. In this study, we evaluated how the photochemical efficiency, cell damage and reproductive biomass of Antarctic pearlwort (Colobanthus quitensis) were affected by different intensities of UV-B ra...
Article
Full-text available
The seed is the most important plant reproductive unit responsible for the evolutionary success of flowering plants. Aside from its essential function in the sexual reproduction of plants, the seed also represents the most economically important agricultural product worldwide, providing energy, nutrients, and raw materials for human nutrition, live...
Article
Asymmetric auxin distribution is instrumental for the differential growth that causes organ bending on tropic stimuli and curvatures during plant development. Local differences in auxin concentrations are achieved mainly by polarized cellular distribution of PIN auxin transporters, but whether other mechanisms involving auxin homeostasis are also r...
Preprint
Full-text available
Oxygenated membrane fatty acid derivatives dubbed oxylipins play important roles in the plant’s defense against biotic and abiotic cues. Plants challenged by insect pests, for example, synthesize a blend of different defense compounds that, amongst others, comprise volatile aldehydes and jasmonic acid (JA). Because all oxylipins are derived from th...
Article
Full-text available
Over the last three decades, novel "omics" platform technologies for the sequencing of DNA and complementary DNA (cDNA) (RNA-Seq), as well as for the analysis of proteins and metabolites by mass spectrometry, have become more and more available and increasingly found their way into general laboratory life. With this, the ability to generate highly...
Article
Full-text available
Potato virus Y is the most economically important potato viral pathogen. We aimed at unraveling the roles of small RNAs (sRNAs) in the complex immune signaling network controlling the establishment of tolerant response of potato cv. Désirée to the virus. We constructed a sRNA regulatory network connecting sRNAs and their targets to link sRNA level...
Preprint
Potato virus Y is the most economically important potato viral pathogen. We aimed at unraveling the roles of small RNAs (sRNAs) in the complex immune signaling network controlling the establishment of tolerant response of potato cv. Désirée to the virus. We constructed a sRNA regulatory network connecting sRNAs and their targets to link sRNA level...
Article
Full-text available
Cycling Dof Factor (CDF) transcription factors (TFs) are involved in multiple processes related to plant growth and development. A member of this family, CDF3, has recently been linked in Arabidopsis to the regulation of primary metabolism and abiotic stress responses, but its role in crop production under stress is still unknown. In this study, we...
Chapter
Over the last few years, it became more and more evident that plant hormone action is to great parts determined through their sophisticated crosstalk, rather than by their isolated activities. Thus, the parallel analysis of interconnected phytohormones in only very small amounts of tissue developed to an important issue in the field of plant scienc...
Data
BiNGO analysis of selected up- and down-regulated genes in cyp79b2/cyp79b3. After filtering the microarray data according to the arbitrary set values of a p ≤ 0.05 and a log2 ratio > 1.2, gene enrichment and clustering in functional groups was analyzed using the BiNGO software package in Cytoscape 3.4.0. Over- and under-represented GO groups are gi...
Data
Functional expression of C-terminally c-myc-tagged NITRILASE 1 from Arabidopsis thaliana. The NIT1:c-myc fusion construct as well as an empty vector control (pEXP1) were expressed in Escherichia coli as outlined in Material and methods. Aliquots of 12.5 μg of protein were subjected to SDS-gel electrophoresis (12.5%) and immunoblotting. The top pane...
Data
Microarray analysis of the cyp79b2/cyp79b3 double mutant in comparison to wild-type Arabidopsis. RNA from 2 week-old cyp79b2/cyp79b3 and wild-type Arabidopsis seedlings was prepared and subjected to microarray analysis using affimetrix Ath1 genome chips. For each genotype three chips were hybridized and the data statistically analyzed employing the...
Data
BiNGO analysis of selected up- and down-regulated genes in cyp79b2/cyp79b3. Graphical representations of over- and under-represented GO groups are provided in two separate figures.
Data
Primary root elongation of wild type, NIT1ox, nit1-3, and NIT2RNAi mutants grown on media containing either IAN or IAA. Seedlings were grown on media containing risings amounts of IAN and IAA, respectively. Since the obtained transgenic lines for NIT1ox and NIT2RNAi showed no phenotypic differences, only data obtained for NIT1ox-J5 and NIT2RNAi-8 a...
Chapter
Technical advances in mass spectrometry constantly raise the bar for analyzing trace amounts of plant hormones in only very small amounts of tissue. Here, a highly sensitive and accurate method is described for the quantitative analysis of the plant hormone salicylic acid not only in the model plant Arabidopsis thaliana but also in other plant spec...
Article
DOF (DNA-binding with one finger)-type transcription factors are involved in many fundamental processes in higher plants, from responses to light and phytohormones to flowering time and seed maturation, but their relation with abiotic stress tolerance is largely unknown. Here, we identify the roles of CDF3, an Arabidopsis DOF gene in abiotic stress...