Steffen Prowe

Steffen Prowe
Beuth Hochschule für Technik Berlin · Department of Biotechnology

Prof. Dr.

About

15
Publications
2,584
Reads
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234
Citations
Citations since 2017
3 Research Items
55 Citations
201720182019202020212022202302468101214
201720182019202020212022202302468101214
201720182019202020212022202302468101214
201720182019202020212022202302468101214
Introduction
Steffen Prowe currently works at the Department of Biotechnology, Beuth Hochschule für Technik Berlin. Steffen does research in Biotechnology and Microbiology. Their current project is 'AdvancedBioPro'. Starting April, he will serve as dean of the department and is interested to connect activities in the area of biotechnology, food technology, phytotechnology, landscape planning and packaging technology.

Publications

Publications (15)
Book
Full-text available
Entwicklung von molekularen Markern und Nachweisverfahren auf Basis der quantitativen PCR für das Monitoring von prozessrelevanten Mikroorganismen als Frühwarnsystem für Prozessstörungen https://www.atb-potsdam.de/publikationen/schriftenreihen/bornimer-agrartechnische-berichte/liste-bisher-erschienener-hefte.html
Conference Paper
Full-text available
Bacterial endospores, mainly of Bacillus subtilis, B. atrophaeus or Geobacillus stearothermophilus, are used as biological indicator for the validation of sterilization processes in various industrial processes and applications. Commonly used spores for sterilization studies are spores of B. subtilis, due to their high degree of resistance to chemi...
Article
Full-text available
Five institutional partners participated in an interlaboratory comparison of nucleic acid extraction, RNA preservation and quantitative Real-Time PCR (qPCR) based assays for biogas biocenoses derived from different grass silage digesting laboratory and pilot scale fermenters. A kit format DNA extraction system based on physical and chemical lysis w...
Data
Full-text available
Five institutional partners participated in an interlaboratory comparison of nucleic acid extraction, RNA preservation and quantitative Real-Time PCR (qPCR) based assays for biogas biocenoses derived from different grass silage digesting laboratory and pilot scale fermenters. A kit format DNA extraction system based on physical and chemical lysis w...
Article
The thermoalkaliphilic anaerobic bacterium Anaerobranca gottschalkii produces an extracellular CGTase when grown on starch at 55 degrees C and pH 9.0. The gene encoding this CGTase was cloned and successfully expressed in Escherichia coli. It encodes a protein consisting of 721 amino acids with a signal sequence of 34 amino acids. On SDS-polyacryla...
Article
Full-text available
A novel thermoalkaliphilic, obligately anaerobic bacterium was isolated from a humid soil sample of a hot inlet of Lake Bogoria, Kenya. The newly isolated strain grows optimally at pH 9.5 and 50-55 degrees C and its growth range is pH 6.0-10.5 and 30-65 degrees C. Unlike the already known thermoalkaliphiles, the strain grows heterotrophically on a...
Article
Three strictly aerobic strains (K-1, K-3d and K-4) were isolated from a hot-spring in Kobe, Japan, and a facultative anaerobic strain LB3A was isolated from sediments collected from the alkaline Lake Bogoria, Kenya. All strains were thermophilic and capable of growth on xylan. On the basis of morphological, physiological and phylogenetic studies th...
Article
Full-text available
Strain LBS3 is a novel anaerobic thermoalkaliphilic bacterium that grows optimally at pH 9.5 and 50°C. Since a high concentration of Na+ ions is required for growth, we have analyzed the primary bioenergetic mechanism of energy transduction in this organism. For this purpose, a method was devised for the isolation of right-side-out membrane vesicle...

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Projects

Projects (3)
Project
Since 2013 I contribute to activities to establish game based microbiology lab course instructions. The aim is to activate student prior to lab course attendance and to get a 1st insight into the scientific as well as technical aspect of the workflow of the upcoming experiments.
Archived project
The aim was to establish qPCR-based systems to monitor the microbiological parameters for biogas plants.
Project
We as Beuth Biotech-Team established an application lab for biotechnological process development. It starts with an automated pipetting technology (PipetMax), involves a biosafety class II lab (safety cabinet), a fully established molecular biology lab (qPCR, PCR-cabinets, separate DNA/DNA-free areas) and a high-throughput robotic device for substance screening and assay automation.