Steffen Ostendorp

Steffen Ostendorp
University of Hamburg | UHH · Biocenter Klein Flottbek and Botanical Garden (BioZ Flottbek)

Doctor of Philosophy

About

13
Publications
3,670
Reads
How we measure 'reads'
A 'read' is counted each time someone views a publication summary (such as the title, abstract, and list of authors), clicks on a figure, or views or downloads the full-text. Learn more
97
Citations
Additional affiliations
April 2019 - March 2020
University of Hamburg
Position
  • PostDoc Position
Description
  • Functional and structural characterization of phloem mobile RBPs
October 2015 - January 2016
University of Hamburg
Position
  • Supervisor
Description
  • Supervisor for student practical courses
July 2013 - present
University of Hamburg
Position
  • PhD Student
Education
October 2010 - March 2013
Universität zu Lübeck
Field of study
  • Molecular Life Science
September 2007 - September 2010
Universität zu Lübeck
Field of study
  • Molecular Life Science

Publications

Publications (13)
Article
Full-text available
Ribosome biogenesis is a key process in all eukaryotic cells that requires hundreds of ribosome biogenesis factors (RBFs), which are essential to build the mature ribosomes consisting of proteins and rRNAs. The processing of the required rRNAs has been studied extensively in yeast and mammals, but in plants much is still unknown. In this study, we...
Article
The HSC70/HSP70 family of heat shock proteins are evolutionarily conserved chaperones involved in protein folding, protein transport, and RNA binding. Arabidopsis HSC70 chaperones are thought to act as housekeeping chaperones and as such are involved in many growth‐related pathways. Whether Arabidopsis HSC70 binds RNA and whether this interaction i...
Article
Full-text available
In higher plants, long-distance RNA transport via the phloem is crucial for communication between distant plant tissues to align development with stress responses and reproduction. Several recent studies suggest that specific RNAs are among the potential long-distance information transmitters. However, it is yet not well understood how these RNAs e...
Preprint
Heat shock proteins of the HSC70/HSP70 family are evolutionarily conserved chaperones that are involved in protein folding, protein transport and RNA binding. Arabidopsis HSC70 chaperones are thought to act as housekeeping chaperones and as such are involved in many growth-related pathways. Whether Arabidopsis HSC70 binds RNA and its function has r...
Chapter
Northern blotting is a classical technique that allows the detection of specific nucleic acids using radioactive or non-radioactive probes. Normally, nucleic acids are denatured and separated by agarose or polyacrylamide gel electrophoresis and transferred and fixed to a membrane prior to detection. Here, we describe a method to analyze specific RN...
Article
Salinity stress is a major abiotic stress that affects plant growth and limits crop production. Roots are the primary site of salinity perception, and salt sensitivity in roots limits the productivity of the entire plant. To better understand salt stress responses in canola, we performed a comparative proteomic analysis of roots from the salt-toler...
Article
Full-text available
Sampling the phloem of higher plants is often laborious and significantly dependent on the plant species. However, proteome studies under denaturing conditions could be achieved in different plant species. Native protein:protein and protein:nucleic acid complexes from phloem samples have as yet scarcely been analyzed, although they might play impor...
Data
Fig. S1 Overview of a 2D gel electrophoresis approach for the identification of protein complexes in oilseed rape phloem sap. Table S1 Oligonucleotides used to detect rRNAs in the phloem sap of Brassica napus Table S2 List of proteins identified by MALDI‐TOF MS from complex I corresponding to the spot numbers in Fig. 2(a) Table S3 List of protei...
Article
Phloem sap contains a large number of macromolecules, including proteins and RNAs from different classes. Proteome analyses of phloem samples from different plant species under denaturing conditions identified hundreds of proteins potentially involved in diverse processes. Surprisingly, these studies also found a significant number of ribosomal and...
Article
Full-text available
Background: Grafting is a well-established technique for studying long-distance transport and signalling processes in higher plants. While oilseed rape has been the subject of comprehensive analyses of xylem and phloem sap to identify macromolecules potentially involved in long-distance information transfer, there is currently no standardised graf...
Article
Full-text available
Collapsin response mediator proteins (CRMPs) are cytosolic phosphoproteins that are mainly involved in neuronal cell development. In humans, the CRMP family comprises five members. Here, crystal structures of human CRMP-4 in a truncated and a full-length version are presented. The latter was determined from two types of crystals, which were either...

Network

Cited By