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Publications (41)
Zymoseptoria tritici, a globally distributed pathogen, is responsible of Septoria tritici
blotch (STB), one of the most damaging wheat diseases. In Italy the incidence of STB has
increased during the past few years. The presence of Z. tritici on flag leaves of susceptible
durum wheat plants, cultivar San Carlo, after a single artificial inoculation...
Zymoseptoria tritici, a globally distributed pathogen, is responsible of Septoria tritici
blotch (STB), one of the most damaging wheat diseases. In Italy the incidence of STB has
increased during the past few years. The presence of Z. tritici on flag leaves of susceptible
durum wheat plants, cultivar San Carlo, after a single artificial inoculation...
Fusarium fujikuroi causes bakanae (“foolish seedling”) disease of rice which is characterized by hyper-elongation of seedlings resulting from production of gibberellic acids (GAs) by the fungus. This plant pathogen is also known for production of harmful mycotoxins, such as fusarins, fusaric acid, apicidin F and beauvericin. Recently, we generated...
PCR validation of telomere-proximal genes in all strains according to reference sequence of strain IMI 58289 (Primer list see S6 Table).
(TIF)
Shoot and root growth of rice seedlings after inoculation with B20 mutants.
Rice seedlings (A-C) and roots (D) inoculated with the gene deletion strains derived from the F. fujikuroi B20 strain. Mock: no fungal inoculation; Δfum1, Δfub1, and Δcps/ks–deletion strains for the fumonisin, fusaric acid and giberellic acid key genes, respectively.
(TIF)
Biosynthesis of gibberellic acids (GA3, GA4, GA7) and fumonisins (FB1, FB2) of stunting-type F. fujikuroi isolates compared to bakanae strains under in vitro (A, B) and in planta (C, D) conditions. GA (A) and fumonisin (B) production levels after 7 days of growth in synthetic medium with 6 mM glutamine. The strains were grown in triplicates. GA (C)...
MRM transitions for liquid culture analysis in negative ionization mode, depicting also transition specific variables.
(DOCX)
MRM transitions for in planta analysis in positive ionization mode, depicting also transition specific variables.
(DOCX)
Shoot (A) and root (B) growth of rice seedlings 7 days after inoculation of high fumonisin-producing F. verticillioides strains. Mock: no fungal inoculation; B14: the F. fujikuroi B14 strain; FvOS35: the F. verticilliodies OS35 strain; FvOS40: the F. verticilliodies OS40 strain.
(TIF)
BUSCO single-copy analysis, performed in gene set (protein) assessment mode on the library Sordariomyceta_odb9.
(DOCX)
Presence of secondary metabolite gene clusters in the analyzed Fusarium strains.
(XLSX)
Rice seed germination assay.
(DOCX)
(A) Expression data and list of all genes with a transcription factor specific InterPro ID in the corresponding protein sequence. (B) Expression data and list of 37 genes which are present in most of the 9 genomes and which are specifically up-regulated during infection of rice. (C) Expression data and list of 28 B14 strain-specific TFs which are n...
Northern blot analysis for comparison of bikaverin (BIK2) and fusarubins (FSR2) gene expression.
The strains were grown for 3 days in synthetic medium with either 6 mM glutamine (bikaverin) or 6 mM NaNO3 (fusarubins) as nitrogen source.
(TIF)
Content and arrangement of genes in gene clusters with variations in the single isolates.
(A) The apicidin F (NRPS31) gene cluster is present in most isolates but missing in strain B14. (B) The fumonisin (PKS11) gene cluster is present in most of the strains, but several genes of the cluster are missing in strain B20. Arrows in blue represent genes...
Rice seedlings inoculated with F. fujikuroi wild-type and mutant strains with or without addition of culture fluids to the pathosystem.
(A) Shoot growth of rice seedlings inoculated with B14 (wild type) and the B14 deletion and complemented strains 9 days after inoculation. (B, C, D) Shoot growth of rice seedlings inoculated with strain B14 (B), B2...
Differentiation of field isolates for their B14-like or B20-like pathotype by PCR and phylogenetic analysis.
(A) Diagnostic PCR amplification using the primer pairs derived from PKS51 (unknown product, 382 bp) and NRPS31 (apicidin F, 434 bp), respectively. (B) Phylogenetic tree constructed by the NJ method using the nucleotide sequences of combined...
Distribution of the mating type loci among the F. fujikuroi isolates.
(XLSX)
Relative transcript levels for the key genes of GA (CPS/KS) and fumonisin (FUM1) biosynthesis.
The transcript levels of CPS/KS (A) and FUM1 (B) were determined by qPCR using total RNA from several F. fujikuroi field isolates grown in ICI liquid medium containing 6 mM glutamine for 7 d. Amplification levels of CPS/KS and FUM1 in the B14 strain were...
Confirmation of the gene deletions (A-F) or complementations (G and H) by PCR. Left panel in each figure: gene deletion or complementation schemes, right panel: PCR gel picture. The genomic positions of the primer pairs (S6 Table) used in the PCR amplification and expected size of PCR products (designated a or b) are indicated in the deletion schem...
PCR primers used in this work.
(DOCX)
MRM transitions for liquid culture analysis in positive ionization mode, depicting also transition specific variables.
(DOCX)
MRM transitions for in planta analysis in negative ionization mode, depicting also transition specific variables.
(DOCX)
An epidemic fungal disease caused by Fusarium proliferatum, responsible for fumonisin production (FB1, FB2, and FB3), has been reported in the main garlic-producing countries in recent years. Fumonisins are a group of structurally related toxic metabolites produced by this pathogen. The aim of this work was to establish an ELISA procedure, mostly a...
Fusarium culmorum is a soilborne fungal pathogen, agent of crown and root rot disease (FCRR), responsible of major economic losses in wheat plants. This host-pathogen interaction, following methyl jasmonate (MeJA) application at the beginning of the necrotrophic stage of infection, has not been previously studied at molecular level. In this study,...
Fusarium Head Blight caused by phytopathogenic Fusarium spp. with Fusarium graminearum as main causal agent is a major disease of durum wheat (Triticum durum Desf.). Mycotoxins in wheat are dominated by trichothecenes B. Fumonisins have only occasionally been reported from wheat; their occurrence was attributed to Fusarium proliferatum and Fusarium...
Fusarium isolates obtained from Iran and Italy were identified by morphological characters and confirmed by using species-specific PCR assays. The genetic chemotyping for each strain, as a preliminary assessment for trichothecene production, was defined using PCR. Subsequently, artificial infection on durum wheat (cv. Normanno) was carried out in t...
Root and crown rot of wheat, caused by Fusarium culmorum (Fc), is a serious disease worldwide, particularly in Iran. Currently, the mechanisms underlying resistance to Fusarium-caused diseases are still unknown. Methyl jasmonate (MeJA) has been identified as a vital cellular regulator, and the effect of exogenous MeJA application during wheat-Fc in...
Wheat is one of the most important crops in Syria, for both local consumption and export commodity and can be infected by Fusarium Head Blight (FHB) a world-widespread disease. Fusarium Head Blight reduces yield, grain quality and causes accumulation of deoxynivalenol (DON). The most promising and effective management strategy is to avail cultivars...
Wheat is one of the most important crops in Syria, for both local consumption and export commodity and can be infected by Fusarium Head Blight (FHB) a world-widespread disease. Fusarium Head Blight reduces yield, grain quality and causes accumulation of deoxynivalenol (DON). The most promising and effective management strategy is to avail cultivars...
During 2011, Fusarium rot of stored garlic was detected on bulbs of ‘Aglio Bianco’ (white garlic) in Piacenza, Ferrara and Rovigo districts. Bulbs, harvested in July, were asymptomatic. During conservation in the drying sheds, approximately thirty percent of bulbs appeared emptied and softened. Fusarium proliferatum was consistently recovered from...
Chemotype composition of Fusarium graminearum strains, isolated from durum wheat kernels from naturally FHB infected fields in Northern and Central Italy, was investigated by multiplex PCR. The different climatic and environmental conditions of the two examined areas separated by the Apennines affected the composition of chemotypes. 15Ac-DON chemot...
Fusarium Head Blight (FHB) is a worldwide disease affecting wheat, barley and other grains, reducing
kernel weight and grain yield; infected seeds may contain a large number of mycotoxins, including trichothecenes
of type A and B. These compounds have already been associated with human and animal toxicoses.
Most common species causing the disease a...
Durum wheat production in Italy is economically of great importance. Fusarium graminearum is the main fusarium head blight (FHB) causal agent in wheat, reducing both yield and grain quality. F. graminearum produces several mycotoxins and, among trichothecenes, deoxynivalenol (DON) and nivalenol (NIV) are the most studied for their toxicity towards...
Several Fusarium species and Microdochium nivale are involved in fusarium head blight (FHB), which in Italy has been constantly present on wheat since 1995. This study was carried out from 1995 to 2007 on FHB-infected durum wheat heads collected in the Bologna countryside, Emilia-Romagna, northern Italy. The most frequent Fusarium species found wer...
Rice (Oryza sativa L.) is cultivated on approximately 230,000 ha in northern Italy. Since 2001, increasing economical losses presumably caused by Fusarium fujikuroi Nirenberg (Gibberella fujikuroi mating population C), an exotic fungus known as the etiological agent of Bakanae disease, have been reported in Italy. The spread of this disease is prim...
Fusarium graminearum is one of the main causal agents of fusarium head blight (FHB) in Italy. A population of 74 F. graminearum strains was collected from symptomatic durum wheat spikes from different fields around Bologna (Emilia-Romagna, northern Italy) between 2006 and 2008. The data obtained showed that the F. graminearum population is variable...
An unusual disease, named elephantiasis for its typical symptoms, has been seen in orchards of kiwifruit cv. Hayward in Emilia-Romagna (northern Italy) since 2001. Phialophora-like isolates were obtained from the necrotic wood and were studied in vitro for phenotype and tissue colonization ability. We used primers amplifying the internal transcribe...
