Stefano Sbrignadello

Italian National Research Council | CNR · Institute of Neuroscience IN

The exposure to different substances present in the environment can affect the ability of the human body to maintain glucose homeostasis. Some review studies summarized the current evidence about the relationships between environment and insulin resistance or beta-cell dysfunction. Instead, no reviews focused on the relationships between the enviro...

Sarcopenia is emerging as a severe complication in type 2 diabetes (T2DM). On the other hand, it has been documented that nutritional aspects, such as insufficient protein or total energy intake, increase sarcopenia risk. The analysis of body composition is a relevant approach to assess nutritional status, and different techniques are available. Am...

Extracellular matrix (ECM) plays a fundamental role in tissue architecture and homeostasis and modulates cell functions through a complex interaction between cell surface receptors, hormones, several bioeffector molecules, and structural proteins like collagen. These components are secreted into ECM and all together contribute to regulate several c...

Background/aims: The prevalence of impaired glucose tolerance (IGT) is rising among obese adolescents in parallel with epidemic obesity. In some cases, IGT reverts to normal glucose tolerance (NGT) by the end of puberty. The aims of the present study were to investigate metabolic factors determining changes over time of glucose at 120 min (Glu120)...

Pancreatic tumors are characterized by marked deposition of extra-cellular matrix, also called desmoplasia, which interacts with tumor cells and facilitates the tumor onset and progression. Thus, it would be relevant to develop a method to quantitatively assess the amount of desmoplasia in images derived from bioptic tissue fragments of the pancrea...

The measurement of glucose levels in patients under life-saving treatments, such as hemodialysis, is clinically important. In fact, during hemodialysis patients with type 2 diabetes may be at risk for hypoglycemic episodes. Even in non-diabetic patients, glycemia monitoring during hemodialysis is relevant, due for instance to its relationships with...

The measurement of glycemia in subjects with renal failure, thus treated with hemodialysis, or peritoneal dialysis, is clinically relevant, since glucose levels may influence the determination of other solutes, such as creatinine, as well as some ions, such as sodium, whose degree of removal during dialysis sessions should be controlled carefully....

CK2 is a multifunctional, pleiotropic protein kinase involved in the regulation of cell proliferation and survival. Since fibroblasts from Type 1 Diabetes patients (T1DM) with Nephropathy exhibit increased proliferation, we studied cell viability, basal CK2 expression and activity, and response to specific CK2 inhibitors TBB (4,5,6,7-tetrabenzotria...

Background: To date, the complexity of the plasma proteome exceeds the analytical capacity of conventional approaches to isolate lower abundance proteins that may prove to be informative biomarkers. Only complex multistep separation strategies have been able to detect a substantial number of low abundance proteins (,100 ng/ml). The first step of th...

In humans, sodium is essential for the regulation of blood volume and pressure. During hemodialysis, sodium measurement is important to preserve the patient from hypo- or hyper-natremia Usually, sodium measurement is performed through laboratory equipment which is typically expensive, and requires manual intervention. We propose a new method, based...

Sodium measurement during hemodialysis treatment is important to preserve the patient from clinical events related to hypo- or hyper-natremia Usually, sodium measurement is performed through laboratory equipment which is typically expensive, and requires manual intervention. We propose a new method, based on conductivity measurement after treatment...

We investigated possible variations of impedance values in samples of sodium chloride solution (sodium chloride 0.9%) with glucose at different concentrations, ranging from 5000 to around 75 mg/dL. The sodium chloride solution (either saline physiological solution) was chosen since it has similarities to blood but no cell components, which may be c...

Aims-The great improvement in the technological aspects of telemedicine has made possible implementing solutions at reasonable costs. Nonetheless, telemedicine applications are still rare. Through a questionnaire addressed to physicians involved in diabetes management, we investigated what is the actual condition of telemedicine in diabetes. Method...

The impact of sex and age on glucose metabolism in the development of overweight/obesity is a matter of debate. We hypothesized that insulin sensitivity (IS) and β-cell function (BF) in a normal white population will differ between males and females and aimed to evaluate the possible effects of BMI and age on metabolic parameters of both sexes. Thi...

Polymeric contamination. Examples of base peak chromatograms obtained after each step of the depletion protocol applied to an ultra-pure water sample. The polymeric contamination was observed after each step of the ProteoPrep20 depletion protocol applied to an ultra-pure water sample. Base Peak Chromatogram of: (A) a water sample; (B) water passed...

Proteins identified with at least 1 peptide and 99% confidence. The accession numbers, and the depletion/enrichment methods applied are also reported. (XLS)

All the peptides belonging to the proteins listed in Table S1 are reported in Table S2, together with their sequences, experimental masses, the difference between calculated and measured masses (expressed in ppm), possible variable modifications, ion scores, expectation values, ranking for Mascot searches, and XCorr, probability and ranking for Seq...

To date, the complexity of the plasma proteome exceeds the analytical capacity of conventional approaches to isolate lower abundance proteins that may prove to be informative biomarkers. Only complex multistep separation strategies have been able to detect a substantial number of low abundance proteins (<100 ng/ml). The first step of these protocol...

We aimed to analyze the shape of the glucose, insulin, and C-peptide curves during a 3-h oral glucose tolerance test (OGTT). Another aim was defining an index of shape taking into account the whole OGTT pattern. Five-hundred ninety-two OGTT curves were analyzed, mainly from women with former gestational diabetes, with glycemic concentrations charac...

In the field of proteomics, several approaches have been developed for separating proteins and analyzing their differential relative abundance. One of the oldest, yet still widely used, is 2-DE. Despite the continuous advance of new methods, which are less demanding from a technical standpoint, 2-DE is still compelling and has a lot of potential fo...

Novel instrumentation for performing large-size (>25 cm) 2-D maps is reported here. To perform the first dimension, we developed a power supply that can deliver a voltage of up to 15,000 V and allows regulation of current (up to 200 μA) onto each individual focusing IPG strip. The IEF strip tray can accommodate up to 12 IPG strips and the electrode...

A novel method for performing 2-D map analysis is here reported, consisting in a modification of the second dimension run, which is performed not in a conventional square- or rectangular-size gel, but in a radial surface. This has the advantage of permitting resolution of closely adjacent bands, representing strings of isoforms of similar or identi...

In recent years there has been considerable interest in the study of glucose-induced dielectric property variations of human tissues as a possible approach for non-invasive glycaemia monitoring. We have developed an electromagnetic sensor, and we tested in vitro its ability to estimate variations in glucose concentration of different solutions with...

Separation of complex mixtures of proteins by 2-DE is a fundamental component of current proteomic technology. Quantitative analysis of the images generated by digitization of such gels is critical for identifying alterations in protein expression within a given biological system. Software packages are designed for this purpose. The accurate defini...

2-DE is a fundamental technology used in proteomics research. However, despite its high capacity to simultaneously separate several proteins for subsequent identification and quantitative comparison studies, a drawback for this technique is its limited reproducibility, especially when comparing data from different laboratories. 2-DE-related variabi...

Minimal model analysis for insulin sensitivity has been validated against the glucose clamp and is an accepted method for estimating insulin sensitivity from IVGTT. However minimal model analysis requires a 3 h test and relevant expertise to run the mathematical model. The aim of this study was to suggest a simple predictor of minimal model analysi...

Impedance spectroscopy has been proposed as possible approach for non-invasive glycaemia monitoring. However, few quantitative data are reported about impedance variations related to glucose concentration variations, especially below the MHz band. Furthermore, it is not clear whether glucose directly affects the impedance parameters or only indirec...

Impedance spectroscopy has been proposed as possible approach for non-invasive glycaemia monitoring. However, few quantitative data are reported about impedance variations related to glucose concentration variations, especially below the MHz band. Furthermore, it is not clear whether glucose directly affects the impedance parameters or only indirec...

Forensic laboratories may be asked to provide genetic evidence that two persons are related or not, when no other relatives are available for study. Sibship analysis of autosome polymorphisms are more complicated since there are no obligatory alleles between siblings that make it possible to exclude a biological relationship with absolute certainty...