Stefano Romano

Stefano Romano
Quadram Institute · Gut Microbes and Health

PhD in Microbiology

About

146
Publications
15,733
Reads
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1,187
Citations
Introduction
Main Research Interests: Comparative genomics Bacterial response to nutrient limitation Metabolomic and proteoemic analysis Production of secondary metabolites Prokaryote-prokaryote and prokaryote-eukaryote interactions
Additional affiliations
September 2010 - February 2014
Max Planck Institute for Marine Microbiology
Position
  • PhD

Publications

Publications (146)

Questions

Questions (8)
Question
Dear all,
I am involved in a small project to investigate the way scientists communicate their work and are engaged in social media. We have created a small survey, which you can find following the link below.
The survey is anonymous, and we calculated that you would need maximum 5 minutes to complete it. I know you are all very busy, but I would be extremely grateful if you could find few minutes to complete the questionnaire.
Thank you very much for your time and your help!
Question
I'm looking for an open source program to open a ".dat" file.
The file is the result of a MASCOT search performed using peptide MS/MS data.
Unfortunately, I did not manage to find any suitable program so far, do you have any suggestions?
Question
I´m working on the identification of a chelating compound produced by a bacterial strain. I have an RP-HPLC enriched fraction and I would like to perform an ESI-MS analysis on it. I would like to analyze the fraction before and after addition of Fe3+ to observe the respective shift in mass and consequently identify the interesting m/z. My fraction is dissolved in 70% acetonitrile. However, I´m a bit concerned about the Fe addition. If I will use Fe2(SO4)3, FeCl3 or any other Fe salt, I will also introduce other salt-ions which could modify the MS scan and ruin the analysis. Do you have any suggestion on how I can add Fe+3 without compromising the MS analysis?
Question
Does anyone have a paper, in which it is indicated how much of the total phosphorous pool in bacteria cells is present in the membrane's phospholipids?

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