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Main Research Interests: Comparative genomics Bacterial response to nutrient limitation Metabolomic and proteoemic analysis Production of secondary metabolites Prokaryote-prokaryote and prokaryote-eukaryote interactions
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I'm looking for an open source program to open a ".dat" file.
The file is the result of a MASCOT search performed using peptide MS/MS data.
Unfortunately, I did not manage to find any suitable program so far, do you have any suggestions?
I´m working on the identification of a chelating compound produced by a bacterial strain. I have an RP-HPLC enriched fraction and I would like to perform an ESI-MS analysis on it. I would like to analyze the fraction before and after addition of Fe3+ to observe the respective shift in mass and consequently identify the interesting m/z. My fraction is dissolved in 70% acetonitrile. However, I´m a bit concerned about the Fe addition. If I will use Fe2(SO4)3, FeCl3 or any other Fe salt, I will also introduce other salt-ions which could modify the MS scan and ruin the analysis. Do you have any suggestion on how I can add Fe+3 without compromising the MS analysis?
Does anyone have a paper, in which it is indicated how much of the total phosphorous pool in bacteria cells is present in the membrane's phospholipids?