Stefano Mariani

Stefano Mariani
Istituto Italiano di Tecnologia | IIT · Center for Convergent Technologies

PhD

About

44
Publications
8,850
Reads
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846
Citations
Additional affiliations
October 2020 - present
Istituto Italiano di Tecnologia
Position
  • PostDoc Position
March 2015 - September 2020
Università di Pisa
Position
  • PostDoc Position
January 2012 - December 2014
University of Florence
Position
  • Ph. D. Candidate

Publications

Publications (44)
Article
Porous silicon (PSi) is a promising material for future integrated nanophotonics when coupled with guest emitters [1,2], still facing challenges in terms of homogenous distribution and nanometric thickness of the emitter coating within the silicon nanostructure. Herein, it is shown that the nanopore surface of a porous silicon oxide (PSiO2) microca...
Article
Full-text available
Here, the authors report on the manufacturing and in vivo assessment of a bioresorbable nanostructured pH sensor. The sensor consists of a micrometer‐thick porous silica membrane conformably coated layer‐by‐layer with a nanometer‐thick multilayer stack of two polyelectrolytes labeled with a pH‐insensitive fluorophore. The sensor fluorescence change...
Article
Nanoparticle-polymer composites hold promise in enabling material functionalities that are difficult to achieve otherwise, yet are hampered to date by the scarce control and tunability of the nanoparticle collective properties on the polymer surface, especially for polymer foams featuring a complex three-dimensional pore network. Here we report on...
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Full-text available
Here the 4D printing of a magnifying polydimethylsiloxane (PDMS) lens encoded with a tunable plasmonic rejection filter is reported. The lens is formed by moldless printing of PDMS pre-polymer on a nanostructured porous silicon (PSi) templating layer. A nanometer-thick plasmonic filter is integrated on the lens surface by in situ synthesis of Ag an...
Article
Full-text available
Plants have evolved different mechanisms to disperse from parent plants and improve germination to sustain their survival. The study of seed dispersal mechanisms, with the related structural and functional characteristics, is an active research topic for ecology, plant diversity, climate change, as well as for its relevance for material science and...
Article
Full-text available
Porous silicon (PSi) is a promising material for future integrated nanophotonics when coupled with guest emitters, still facing challenges in terms of homogenous distribution and nanometric thickness of the emitter coating within the silicon nanostructure. Herein, it is shown that the nanopore surface of a porous silicon oxide (PSiO2) microcavity (...
Article
Full-text available
Here, a fluoride‐assisted route for the controlled in‐situ synthesis of metal nanoparticles (NPs) (i.e., AgNPs, AuNPs) on polydimethylsiloxane (PDMS) is reported. The size and coverage of the NPs on the PDMS surface are modulated with time and over space during the synthetic process, leveraging the improved yield (10×) and faster kinetics (100×) of...
Article
The increasing request of on-chip energy storage devices is driven by the augmented connectivity between people and things for IoT, portable, and wearable electronic applications. These systems require high performance components with low power consumption, compact size, and high energy storage capability. Supercapacitors (SCs) achieve capacitance...
Article
Here we report the layer-by-layer (LbL) nanoassembly of charged polyelectrolytes engineered with bioreceptors as an effective and robust alternative to covalent biofunctionalization (e.g., organosilanization and hydrosilylation) for affinity biosensing with porous silicon (PSi) interferometers [1]. As a proof of concept demonstration, a bi-layer of...
Article
In this work [1], we describe the moldless preparation of a poly(dimethylsiloxane) (PDMS) lens embedding nanostructured porous silicon (PSi) optical components. Casting of uncured PDMS onto a PSi surface shapes a droplet with contact angle easily controllable by tuning of the nanostructure features (i.e., thickness and porosity of PSi). Design of t...
Article
The currently growing request for a continuous connectivity between people and things has increased exponentially the demand for portable and/or wearable electronics. Thus, high performance components with more compact size, lower power consumption, and increased energy storage capability are needed. Although electronic active components have downs...
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Full-text available
Microneedle (MN), a miniaturized needle with a length‐scale of hundreds of micrometers, has received a great deal of attention because of its minimally invasive, pain‐free, and easy‐to‐use nature. However, a major challenge for controlled long‐term drug delivery or biosensing using MN is its low tissue adhesion. Although microscopic structures with...
Article
Full-text available
Over the last decade, scientists have dreamed about the development of a bioresorbable technology that exploits a new class of electrical, optical, and sensing components able to operate in physiological conditions for a prescribed time and then disappear, being made of materials that fully dissolve in vivo with biologically benign byproducts upon...
Article
Full-text available
In this work, both light‐shaping and image magnification features are integrated into a single lens element using a moldless procedure that takes advantage of the physical and optical properties of mesoporous silicon (PSi) photonic crystal nanostructures. Casting of a liquid poly(dimethylsiloxane) pre‐polymer solution onto a PSi film generates a dr...
Article
Capacitors are the most critical passive components of future in-package and on-chip electronic systems with augmented energy-storage capabilities for consumer and wearable applications. Although an impressive increase of both capacitance and energy densities has been achieved over the last years for supercapacitors (SCs), electronic applications o...
Article
Gold nanoparticle layers (AuNPLs) enable the coupling of morphological, optical and electrical properties of AuNPs in liquid dispersion with tailored and specific surface topography, making them exploitable in many bio-applications (e.g. biosensing, drug delivery, photothermal therapy). Herein, we report the formation of AuNPLs on porous silicon (P...
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Full-text available
Nanostructured materials premise to revolutionize the label-free biosensing of analytes for clinical applications, leveraging the deeper interaction between materials and analytes with comparable size. However, when the characteristic dimension of the materials reduces to the nanoscale, the surface functionalization for the binding of bioreceptors...
Article
Herein, we report low concentration ethanol vapor detection with nanostructured porous silicon (PSi) interferometers using Interferogram Average over Wavelength Reflectance Spectroscopy (IAWRS), a novel interferometric technique recently developed for ultrasensitive (bio)sensing applications in liquid using miniaturized integrated interferometers....
Article
Low-cost piezoresistive strain/pressure sensors with large working range, at the same time able to reliably detect ultralow strain (≤0.1%) and pressure (≤1 Pa), are one of the challenges that have still to be overcome for flexible piezoeresistive materials towards personalized health-monitoring applications. In this work, we report on unprecedented...
Article
Herein, we provide the first experimental evidence on the use of electrical double layer (EDL)-induced accumulation of charged ions (using both Na+ and K+ ions in water as model) onto a negatively-charged nanostructured surface (e.g. thermally growth SiO2) – Ion Surface Accumulation, ISA – as a means of improving performance of nanostructured porou...
Article
The prion protein (PrPC) can be structurally shifted to its PrPSc isoform causing a wide range of neurodegenerative diseases, which are currently incurable. There is an evidence that metallothioneins (MTs), and especially MT-3, are associated with neurodegenerative diseases. PrPC and MTs play pivotal roles in maintaining metal homeostasis; therefor...
Article
Here we present a new generic opto-bio-sensing platform combining immobilised aptamers on an infrared plasmonic sensing device generated by nano-structured thin film that demonstrates amongst the highest index spectral sensitivities of any optical fibre sensor yielding on average 3.4 × 10 4 nm/RIU in the aqueous index regime (with a figure of merit...
Article
On-field analysis (e.g. clinical and diagnostics) of nanostructured porous silicon (PSi) for label-free optical biosensing has been so far hindered by insufficient sensitivity of PSi biosensors. Here we report on a label-free PSi interferometric aptasensor able to specifically detect Tumor Necrosis Factor alpha (TNFα, a protein biomarker of inflamm...
Article
Nanostructured porous silicon (PS) is a promising material for the label-free optical detection of biomolecules, but it currently suffers of limited clinical diagnostic applications due to insufficient sensitivity. In this regard, here we introduce an ultrasensitive and robust signal processing strategy that relies on the calculation of the average...
Article
Full-text available
In this paper real-time, label free sensing principle based on Surface Plasmon Resonance (SPR) and gravimetric, i.e. Quartz Crystal Microbalance (QCM) are described and some applications to food analysis problems are reported. Affinity receptors are introduced and examples using antibodies, nucleic acid probes and biomimetic receptors i.e. molecula...
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Full-text available
Nanoparticles with different sizes, refractive indices and plasmonic profiles are synthesized, labelled to DNA and embedded in DNA based plasmonic nanoarchitectures. The contributions of the different properties on Reflectivity Variations % are rationally investigated by DNA hybridization measurements with Surface Plasmon Resonance imaging (SPRi) t...
Article
Full-text available
Optical genotyping of C3435T single nucleotide polymorphisms (SNPs) in unamplified human multidrug re-sistance (MDR1) gene was here performed by a surface plas-mon resonance imaging (SPRi) dual-targeting DNA assay, allowing its selective detection down to 0.18 fM of the whole genomic DNA. The result was achieved by the combination of the rational s...
Article
Full-text available
In the last 20 years, surface plasmon resonance (SPR) and its advancement with imaging (SPRi) emerged as a suitable and reliable platform in clinical analysis for label-free, sensitive, and real-time monitoring of biomolecular interactions. Thus, we report in this review the state of the art of clinical target detection with SPR-based biosensors in...
Article
Surface Plasmon Resonance imaging (SPRi) is an advanced optical transducer for the DNA affinity biosensors. SPRi signal enhancement of a DNA biosensor is a goal in developing innovative devices, especially for clinical applications. Nanoparticles (NPs) play a forefront role in signal enhancement in biosensor-based analysis, using different transduc...
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Full-text available
The use of nanoparticles (NPs) can substantially improve the analytical performance of surface plasmon resonance imaging (SPRi) in general, and in DNA sensing in particular. In this work, we report on the modification of the gold surface of commercial biochips with gold nanospheres, silica-coated gold nanoshells, and silver nanoprisms, respectively...
Article
The direct detection of specific sequences in genomic DNA samples is very challenging in the biosensor-based approach. In this work we developed an optimized strategy for the direct detection of DNA sequences in human genomic samples by a surface plasmon resonance imaging technology. As model study, the target analyte was identified in a DNA sequen...
Article
Full-text available
The work presented here deals with the optimization of a strategy for detection of single nucleotide polymorphisms based on surface plasmon resonance imaging. First, a sandwich-like assay was designed, and oligonucleotide sequences were computationally selected in order to study optimized conditions for the detection of the rs1045642 single nucleot...

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Projects

Project (1)
Project
The Sens4Bio project addresses the novel paradigm of "flow-through" optofluidics for the realization and assessment of ultra-sensitive resonant optical sensors, by also exploiting novel functionalization schemes, such as synthetic polymeric receptors and nanoparticle-based systems for further pushing sensitivity and limit-of-detection (LOD) beyond the state-of-the-art on optical sensors at national and international level. The main objective of The Sens4Bio project is the development of three "flow-through" optofluidic microresonators, based on different operation principles and on different fabrication technologies, and two functionalization strategies, integrating biological and polymeric receptors with the optofluidic microresonators for the realization of ultra-sensitive optofluidic sensors.