Stefanie Wienkoop

Stefanie Wienkoop
University of Vienna | UniWien · Department of Functional and Evolutionary Ecology

Professor

About

160
Publications
17,646
Reads
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Introduction
Stefanie Wienkoop investigates plant-microsymbiont interactions. Therefor she integrates techniques from genome to proteome to metabolome as well as plant phenotyping. Her group is particularly interested in symbiosome membrane proteins and the symbiont induced symbio-proteome involved in drought stress alleviation. http://homepage.univie.ac.at/stefanie.wienkoop/index.html
Additional affiliations
December 2017 - July 2020
University of Vienna
Position
  • Professor (Associate)
Description
  • Plant microsymbiont interaction
October 2008 - November 2017
University of Vienna
Position
  • Professor
January 2007 - September 2008
Universität Potsdam
Position
  • PostDoc Position

Publications

Publications (160)
Article
Full-text available
Drought stress hampers plant energy and biomass production; however it is still unknown how internal C:N balance and rhizobial symbiosis impact on plant response to water limitation. Here, the effect of differential optimal nitrogen nutrition and root nodule symbiosis on drought stress and rehydration responses of Medicago truncatula was assessed....
Article
Full-text available
Climate change in conjunction with population growth necessitates a systems biology approach to characterize plant drought acclimation as well as a more thorough understanding of the molecular mechanisms of stress recovery. Plants are exposed to a continuously changing environment. Extremes such as several weeks of drought are followed by rain. Thi...
Article
Full-text available
Unlabelled: The long cultivation of field pea led to an enormous diversity which, however, seems to hold just little resistance against the ascochyta blight disease complex. The potential of below ground microbial symbiosis to prime the immune system of Pisum for an upcoming pathogen attack has hitherto received little attention. This study invest...
Article
Full-text available
In field peas, ascochyta blight is one of the most common fungal diseases caused by Didymella pinodes. Despite the high diversity of pea cultivars, only little resistance has been developed until to date, still leading to significant losses in grain yield. Rhizobia as plant growth promoting endosymbionts are the main partners for establishment of s...
Article
Full-text available
Legume‐rhizobia symbioses play a major role in food production for an ever growing human population. In this symbiosis, dinitrogen is reduced ('fixed') to ammonia by the rhizobial nitrogenase enzyme complex and is secreted to the plant host cells, while dicarboxylic acids derived from photosynthetically‐produced sucrose are transported into the sym...
Article
In plants, flooding-induced oxygen deficiency causes severe stress, leading to growth reduction and yield loss. It is therefore important to understand the molecular mechanisms for adaptation to hypoxia. Aquaporins at the plasma membrane play a crucial role in water uptake. However, their role during hypoxia and membrane redox changes is still not...
Article
Full-text available
During moderate drought stress, plants can adjust by changes in the protein profiles of the different organs. Plants transport and modulate extracellular stimuli local and systemically through commonly induced inter-and intracellular reactions. However, most proteins are frequently considered, cell and organelle specific. Hence, while signaling mol...
Article
Full-text available
Flooding induces low-oxygen environments (hypoxia or anoxia) that lead to energy disruption and an imbalance of reactive oxygen species (ROS) production and scavenging enzymes in plants. The influence of hypoxia on roots of hydroponically grown maize (Zea mays L.) plants was investigated. Gene expression (RNA Seq and RT-qPCR) and proteome (LC–MS/MS...
Article
Full-text available
The multinational Arabidopsis research community is highly collaborative and over the past thirty years these activities have been documented by the Multinational Arabidopsis Steering Committee (MASC). Here, we (a) highlight recent research advances made with the reference plant Arabidopsis thaliana ; (b) provide summaries from recent reports submi...
Article
Full-text available
Pulses are one of the most important categories of food plants, and Pea (Pisum sativum L.) as a member of pulses is considered a key crop for food and feed and sustainable agriculture. Integrative multi-omics and microsymbiont impact studies on the plant's immune system are important steps toward more productive and tolerant food plants and thus wi...
Chapter
Due to future resource limitations and the enormous amounts of energy used for fertilizer production, symbiosis with rhizobia that possess the capacity to fix atmospheric nitrogen is gaining agricultural relevance for pulses. The progressively occurring events of drought, and soil degradation through increasing salinity levels, are a race against t...
Chapter
Legume crops present important agronomical and environmental advantages mainly due to their capacity to reduce atmospheric N2 to ammonium via symbiotic nitrogen fixation (SNF). This process is very sensitive to abiotic stresses, such as drought, but the mechanism underlying this response is not fully understood. The goal of the current work was to...
Article
Nitric oxide (NO) is a crucial signaling molecule that conveys its bioactivity mainly through protein S-nitrosylation. This is a reversible post-translational modification (PTM) that may affect protein function. S-nitrosoglutathione (GSNO) is a cellular NO reservoir and NO donor in protein S-nitrosylation. The enzyme S-nitrosoglutathione reductase...
Article
Full-text available
Cork is a renewable non-wood high valued forest product, with relevant ecological and economic impact in the Mediterranean-type ecosystems. Currently, cork is ranked according to its commercial quality. The most valuable planks are chosen for cork stoppers production. Cork planks with adequate thickness and porosity are classified as stoppable qual...
Article
Full-text available
Plant phenotyping to date typically comprises morphological and physiological profiling in a high-throughput manner. A powerful method that allows for subcellular characterization of organelle stoichiometric/functional characteristics is still missing. Organelle abundance and crosstalk in cell dynamics and signaling plays an important role for unde...
Article
Full-text available
Tungsten (W) finds increasing application in military, aviation and household appliance industry, opening new paths into the environment. Since W shares certain chemical properties with the essential plant micronutrient molybdenum (Mo), it is proposed to inhibit enzymatic activity of molybdoenzymes [e.g., nitrate reductase (NR)] by replacing the Mo...
Data
Figure S1. Light micrographs of a nodule of L. japonicus. Nodule semi‐thin sections (1 μm) were collected on glass slides and stained with toluidine blue. (a) wt plants. (b) sst1 mutant showing lower density of infected cells (blue) and signs of elevated cytoplasmic vacuolation and lysis of bacteroids. Scale bars, 75 μm. Figure S2. Relative quanti...
Data
Table S1a. MaxQuant information on protein and peptide identification used in Figure 3 and Figures S2 and S3. Table S1b. MaxQuant information of protein Label‐Free Quantification (LFQ intensities) used in Figures S2 and S3. rep, biological replicate; ND, not determined. Table S2a. Data matrix of RIA value calculations, extracted from the MS analy...
Article
Full-text available
Most crop plants are exposed to intermittent drought periods. To cope with these continuous changes, plants need strategies to prevent themselves from exhaustive adjustment maneuvers. Drought stress recovery has been shown to be an active process, possibly involved in a drought memory effect allowing plants to better cope with recurrent aridity. An...
Data
Information about the selected RNA primers of the target proteins used for qPCR.
Data
List of highest loadings for IC1 (a) and IC3 (b) corresponding to ICA, Figure 3.
Data
Beta vulgaris leaf proteomics data. Identified proteins, mapped functional categories, ratio of mean LFQ intensities per paired comparison of treatments (n = 4) and ANOVA (p-val < 0.05 green; FC–ratios > 2 red; FC–ratios < 2 yellow ); “not valid” if too many missing values for statistics in one of the treatments (n < 4).
Data
Time course of different physiological parameters (RWC: relative water content; OP: osmotic potential; EL: electrolyte leakage) and metabolites (hexoses: sum of fructose and glucose) during stress and recovery. Values represent ratios of stressed to control plants of the same day. Asterisks indicate statistically significant differences (controls v...
Data
Venn diagrams of the numbers of statistically (p < 0.05; Kruskal-Wallis) significantly changed proteins overlapping between D9, D11 and DR11.
Article
Full-text available
In pea (Pisum sativum L.) production, Didymella pinodes (Berk. & A. Bloxam) Petr. is the most damaging aerial pathogen globally. In two completely randomized pot experiments with four replicates, we studied the effects of D. pinodes infection interaction with three symbiotic treatments (Rhizobium leguminosarum biovar viciae, arbuscular mycorrhizal...
Article
Full-text available
Sulfur is an essential nutrient in plants as a constituent element of some amino acids, metal cofactors, coenzymes, and secondary metabolites. Not surprisingly, sulfur deficiency decreases plant growth, photosynthesis, and seed yield in both legumes and non-legumes. In nodulated legumes, sulfur supply is positively linked to symbiotic nitrogen fixa...
Article
Full-text available
Legume plants are key elements in sustainable agriculture and represent a significant source of plant-based protein for humans and animal feed worldwide. One specific feature of the family is the ability to establish nitrogen-fixing symbiosis with Rhizobium bacteria. Additionally, like most vascular flowering plants, legumes are able to form a mutu...
Article
Full-text available
Plant diseases caused by viral infection are affecting all major crops. Being an obligate intracellular organisms, chemical control of these pathogens is so far not applied in the field except to control the insect vectors of the viruses. Understanding of molecular responses of plant immunity is therefore economically important, guiding the enforce...
Article
Full-text available
Legumes are a large and economically important family, containing a variety of crop plants. Alongside different cereals, some fruits, and tropical roots, a number of leguminosae evolved for millennia as crops with human society. One of these legumes is Pisum sativum L., the common garden pea. In the past, breeding has been largely selective on impr...
Article
Full-text available
Ascochyta blight causes severe losses in field pea production and the search for resistance traits towards the causal agent Didymella pinodes is of particular importance for farmers. Various microsymbionts have been reported to shape the plants' immune response. However, regardless their contribution to resistance, they are hardly included in exper...
Conference Paper
Full-text available
Species of ascochyta blight such as Didymella pinodes is one of the most damaging aerial pathogen of field pea (Pisum sativum. L) (Fondevilla et al., 2013; Tivoli and Banniza, 2007). It sometimes results in the loss of the entire crop, especially in monoculture and whenever there is a favourable environmental condition for its infection and further...
Article
Abiotic stresses caused by adverse environmental conditions are responsible for heavy economic losses on pea crop, being drought one of the most important abiotic constraints. Development of pea cultivars well adapted to dry conditions has been one of the major tasks in breeding programs. The increasing food requirements drive the necessity to broa...
Article
During arbuscular mycorrhizal symbiosis, arbuscule-containing root cortex cells display a proliferation of plastids, a feature usually ascribed to an increased plant anabolism despite the lack of studies focusing on purified root plastids. In this study, we investigated mycorrhiza-induced changes in plastidic pathways by performing a label-free com...
Article
In this work flour samples of various cereal species, a group of different cultivars of small grain species (wheat, Triticum spp.; barley, Hordeum vulgare; oat, Avena sativa; rye, Secale cereale; triticale, Triticosecale) and cultivars of corn species, Zea Mays, growing in the same period and in the same geographical area, were selected to establis...
Article
Significance: Pea (Pisum sativum L.) belongs to the legume family (Fabaceae) and is an important crop plant due to high Fe, starch and protein contents. According to FAOSTAT data (September 2015), world production of the garden pea quadrupled from 1970 to 2012. Since the initial studies by Gregor Mendel, the garden pea became the most-characterize...
Article
The proteomic study of non-model organisms, such as many crop plants, is challenging due to the lack of comprehensive genome information. Changing environmental conditions require the study and selection of adapted cultivars. Mutations, inherent to cultivars, hamper protein identifi cation and thus considerably complicate the qualitative and quanti...