Stefanie Reim

Stefanie Reim
Julius Kühn-Institute, Dresden, Germany · Institute for Breeding Research on Fruit Crops

PhD

About

41
Publications
6,235
Reads
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336
Citations
Introduction
Stefanie Reim currently works at the Institute for Breeding Research on Fruit Crops, Julius Kühn-Institute, Dresden, Germany. Stefanie does research in fruit genetic resources and fruit breeding using several molecular and phenotypic methods.
Additional affiliations
March 2017 - April 2020
Julius Kühn-Institut
Position
  • Researcher
Description
  • • Phenotyping, including digital methods • Genotyping by Sequencing (SSR, GBS) and genetic mapping • Gene expression analysis (qRT-PCR, Biomark HD, Fluidigm) • RNA sequencing • In vitro culture
September 2013 - June 2017
Sächsisches Staatsministerium für Umwelt und Landwirtschaft
Position
  • Project Manager
Description
  • Project: 'Preserving the intraspecific diversity of native wild fruits in Saxony' • Genetic, phenotypic and phenological evaluation, GIS mapping • Population genetic analysis • In situ and ex situ conservation measures
May 2011 - July 2012
Riboxx
Riboxx
Position
  • Research Associate
Description
  • • Scientific supervision of international research projects • preparation of scientific reports, presentations and publications • Scientific and administrative support for distributors and customers
Education
October 1994 - June 2001
University of Rostock
Field of study
  • Agriculture Ecology

Publications

Publications (41)
Article
Full-text available
In the present study genetic diversity and hybridization with cultivars were investigated in a population of the endangered European wild apple species Malus sylvestris (L.) Mill. with the aim to establish a basis for the implementation of conservation activities and to ensure its long-term preservation. A total of 284 putative M. sylvestris trees...
Poster
Full-text available
Phenotyping and monitoring of phytopathogens are increasingly challenge for fruit breeding Phenotyping of plant diseases is usually carried out manually, but this is very labor and cost intensive Therefore, only a selected number of fruit trees in a limited period of time are usually phenotyped Another disadvantage is that the infestation is not al...
Article
Full-text available
Apple replant disease (ARD) is a worldwide economic risk in apple cultivation for fruit tree nurseries and fruit growers. Several studies on the reaction of apple plants to ARD are documented but less is known about the genetic mechanisms behind this symptomatology. RNA-seq analysis is a powerful tool for revealing candidate genes that are involved...
Article
Full-text available
The tea collection of the FRC SSC RAS (Sochi, Maykop in Russia) represents one of the northernmost germplasm comprising a number of locally derived cultivars and G-irradiation mutants. The latter are often characterized by larger genome size, which may lead to better adaptation to biotic and abiotic stress. Such genotypes may be a valuable genetic...
Article
Full-text available
Characterization of genetic diversity in germplasm collections requires an efficient set of molecular markers. We assessed the efficiency of 36 new SCoT markers, 10 new ISSR markers, and 5 microsatellites for the characterization of genetic diversity in chrysanthemum core collection of 95 accessions (Russian and foreign cultivars). Seven new SCoT (...
Article
Characterization of genetic diversity in germplasm collections is fundamental for an efficient management and utilization. The citrus germplasm in the Western Caucasus represent one of the most northerly citrus collections. The collection contains numerous cultivars specifically selected for frost-tolerance, which are valuable as genetic resources...
Poster
Full-text available
Apple Replant Disease (ARD) is a serious disease of economic importance in apple orchards. Several studies on the reaction of apple plants to ARD are documented but less is known about the genetic mechanisms involved in tolerance/susceptibility to the disease. RNA-Seq analysis is a powerful tool for revealing candidate genes that are putatively inv...
Article
Full-text available
Simple Summary: Persimmon is an edible fruit consisting of several species in the genus Diospyros. The most widely cultivated and most commercially important species is the Oriental persimmon, Diospyros kaki. However, the inter-and intra-specific genetic diversity of the genus Diospyros remains largely unclear and is of great interest both for cons...
Article
Full-text available
Malus sylvestris (Mill.) is the only indigenous wild apple species in Central Europe. Agriculture, forestry, and urbanization increasingly endanger Malus sylvestris natural habitats. In addition, the risks of cross-hybridization associated with increase in the cultivation of the domesticated apple Malus × domestica (Borkh.) threaten the genetic int...
Article
Full-text available
Apple replant disease (ARD) is a soil-borne disease, which is of particular importance for fruit tree nurseries and fruit growers. The disease manifests by a poor vegetative development, stunted growth, and reduced yield in terms of quantity and quality, if apple plants (usually rootstocks) are replanted several times at the same site. Genotype-spe...
Poster
Full-text available
The poster shows the first results of (I) the validation of candidate genes responding to a tolerance to Apple Replant Disease (ARD) as possible biomarkers for ARD tolerance/susceptibility and (II) RNA sequencing for the identification of new candidate gene affecting a tolerance to ARD .
Article
Replant disease, also described as soil decline, is known for centuries; yet the ultimate causes remain unknown. After repeated growing of the same plant species, the soil loses its capacity to support the growth of plants of the respective species. Replant disease is characterized by a poor vegetative development, stunted growth and reduced yield...
Article
Full-text available
Nach der Neuregelung des Bundesnaturschutzgesetzes (§ 40 Abs. 4 Satz 4 BNatSchG) ist ab dem 1. März 2020 das Ausbringen von Gehölzen und Saatgut in der freien Natur außerhalb ihrer Vorkommensgebiete genehmigungspflichtig. Eine wesentliche Voraussetzung für die Ausbringung herkunftsgesicherten Pflanz- und Saatguts ist die Gewinnung genetisch hochwer...
Poster
Full-text available
The poster presents the first results of the Evaluation of Malus genetic resources for tolerance to apple replant disease (ARD)
Article
Im Rahmen eines Modellvorhabens zur Erhaltung und Förderung seltener Wildobstgehölze wurden natürliche Wildbirnenbestände im gesamten Gebiet des Freistaates Sachsen gesucht und kartiert. Reichlich 320 potentielle Wildbirnen in sieben Beständen wurden gefunden, dabei wurden von 313 Bäumen Blattproben entnommen und genetisch untersucht. Mithilfe gene...
Article
After replanting apple (Malus domestica Borkh.) on the same site severe growth suppressions, and a decline in yield and fruit quality are observed in all apple producing areas worldwide. The causes of this complex phenomenon, called apple replant disease (ARD), are only poorly understood up to now which is in part due to inconsistencies in terms an...
Article
Full-text available
The present study investigated genetic diversity, structure and hybridization in a collection of the endangered wild pear species Pyrus pyraster (L.) Burgd. A total of 278 putative ‘true type’ P. pyraster trees originating from seven populations in the federal state of Saxony in Germany were analyzed along with 35 pear cultivars commonly cultivated...
Article
Full-text available
In Deutschland gibt es nur noch wenige Vorkommen von M. sylvestris und P. pyraster, weshalb die langfristige Sicherung dieser beiden Arten ex-situ mit Hilfe von Erhaltungsplantagen sinnvoll ist. In der Regel umfassen Erhaltungsplantagen nur eine Auswahl der Genotypen (Kernsammlung) aus den Ausgangspopulationen (Basissammlung), da aus Kostengründen...
Article
Full-text available
In recent years, land use changes led to a rapid decline and fragmentation of J. communis populations in Germany. Population isolation may lead to a restricted gene flow and, further, to negative effects on genetic variation. In this study, genetic diversity and population structure in seven fragmented J. communis populations in Saxony, Germany, we...
Conference Paper
Full-text available
Die Grüne Liga Osterzgebirge e.V. und der Staatsbetrieb Sachsenforst bearbeiten seit Juli 2012 das Modell- und Demonstrationsvorhaben „Erhaltung der innerartlichen Vielfalt gebietsheimischer Wildobstarten in Sachsen“. Das Hauptanliegen des Projektes ist, durch die Etablierung von In-situ Erntebeständen seltene Wildobstarten dauerhaft zu erhalten un...
Article
Full-text available
Durch veränderte Nutzungsformen sind die vorhandenen Wacholderstandorte in Deutschland stark rückläufig. Für Gesamtdeutschland wurde der Wacholder daher in die Vorwarnliste der gefährdeten Arten aufgenommen. In Sachsen gilt die Art als stark gefährdet. Das Biosphärenreservat Oberlausitzer Heide- und Teichlandschaft umfasst eines der wenigen größer...
Article
Full-text available
Knowledge of pollen movement and frequency of interspecific hybridization in fragmented populations of rare species is a prerequisite for the implementation of conservation measures. In a large-scale study area (14,000 hectares) we analysed 297 Malus sylvestris trees with nine nuclear microsatellite markers. After open pollination of 564 offspring...
Article
Full-text available
Malus sylvestris (L.) Mill. is the only indigenous wild apple species in middle Europe. The crab apple is only rarely found in its distribution area and increasingly endangered. Therefore protective measures are necessary to ensure its sustainable preservation. In a four year project, supported by the German Federal Agency for Agriculture and Food,...
Article
Malus sylvestris (L.) Mill, is the only indigenous wild apple species in middle Europe. The crab apple is only rarely found in its distribution area and increasingly endangered. Therefore protective measures are necessary to ensure its sustainable preservation. In a four-year project, supported by the German Federal Agency for Agriculture and Food,...
Article
Full-text available
In the present study genetic diversity and hybridization with cultivars were investigated in a population of the endangered European wild apple species Malus sylvestris (L.) Mill. with the aim to establish a basis for the implementation of conservation activities and to ensure its long-term preservation. A total of 284 putative M. sylvestris trees...
Article
Full-text available
Malus sylvestris (L.) Mill. is the only indigenous wild apple species in Europe and recognized as rare and endangered species. An important prerequisite for the preservation of M. sylvestris is the identification of ‘true type’ M. sylvestris individuals because hybrids with cultivated apple could be also occurred. In this study variations among 625...
Article
Little is known about the genetic diversity of seedless barberry cultivars and its wild type relatives. Microsatellite markers next to the morphological traits were used to distinguish the relatedness of some 47 genotypes; forty-five Iranian genotypes including both seedless cultivars and wild types and two foreign genotypes: Berberis vulgaris and...
Article
Full-text available
Dry fruits of seedless barberry cultivars and its wild types are used as food, industrial and for medicinal purposes in Iran. There is little information about the genetic diversity of the wild types and the origin of seedless barberry cultivars. This study was aimed on the identification of genetic relationship between barberry genotypes using mic...
Article
A lot of apple cultivars have been released in Dresden-Pillnitz. Two different series have been developed: the Pi- and the Re-series. Pi- as prefix indicate that cultivars were bred mainly for quality (Pi for Pillnitz) whereas Re- as prefix indicate the main aim resistance (Re for resistant). Although in apple breeding the protection of flowers wit...
Article
Full-text available
The generation of transgenic apple plants relies on the molecular analysis of transgene integration and expression based on polymerase chain reaction (PCR) analysis, blotting techniques and enzymatic assays on vitro leaves of putative transgenic regenerates. In order to assess the uniformity and the stability of transfer DNA (T-DNA) integration and...
Article
The generation of transgenic apple plants relies on the molecular analysis of transgene integration and expression based on polymerase chain reaction (PCR) analysis, blotting techniques and enzymatic assays on vitro leaves of putative transgenic regenerates. In order to assess the uniformity and the stability of transfer DNA (T-DNA) integration and...
Article
Putative transgenic plants are commonly analyzed for integration and expression of the transferred genes by molecular techniques. Most of these techniques cannot distinguish between plants containing a mixture of transformed and non-transformed cells (chimera) and plants containing only transgenic cells (uniform). Putative trans-genics with detecta...
Article
Full-text available
The release of genetically engineered apple trees raises the question of their potential environmental impact, and the transfer via pollen of transgenes to cross-compatible cultivars of Malus domestica and Malus species is deemed to be the greatest source for environmental exposure. The hybrid TNR 31-35, a descendant of Malus sieversii var. sievers...
Article
With respect to the release of transgenic woody plants the stability of integrated transgenes and their long-term expression is one of the main objectives in monitoring and risk assessment of genetically engineered plants. A stable integration and expression of the transgenes is particular important for fruit trees, which have an exceptional long p...

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Projects

Projects (5)
Project
The project is aimed on the establishment of a digital monitoring system for the early detection and localization of quarantine diseases in commercial fruit orchards. For this purpose, large numbers of spatially high-resolution images of species-specific disease symptoms will be recorded at different stages of development in apple and pear orchards. Initially, the focus is on detection of leaf symptoms. Infected plants can be located accurately using Global Positioning System (GPS) within the area. In addition, the course of the diseases will be connected with geographic information from the weather services. The long-term goal of the project is the establishment of a labor-saving and cost-effective high-throughput process as a control system for fruit production and breeding, which enables continuous spatial recording and documentation of infections caused by quarantine pathogens.
Archived project
Replant disease is an economical important soil-borne disease in tree nurseries and fruit orchards. Up to now the ultimate causes are unknown. The identification of ARD tolerant genotypes and the understanding of its molecular basis are prerequisites for future rootstock breeding.
Project
Existing digital phenotyping methods will be evaluated regarding their usability in fruit breeding research. The focus is on digital phenotyping methods that support apple and cherry breeding, breeding research and evaluation of fruit genetic resources. Suitable methods should be established and if necessary new methods of digital phenotyping developed.