Stefanie Gilles

Stefanie Gilles
Universität Augsburg | UNA · Faculty of Medicine Dept. of Environmental Medicine

PD Dr.

About

97
Publications
11,506
Reads
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2,064
Citations
Introduction
Stefanie Gilles currently works as senior scientist at the Department of Environmental Medicine, Faculty of Medicine, University of Augsburg, Germany. Stefanie does research in Immunology, Allergology and Clinical Immunology. Current project is 'Allergenicity of pollen'
Additional affiliations
January 2021 - present
University of Augsburg Medical Faculty
Position
  • Group Leader
January 2014 - present
Technische Universität München
Position
  • PostDoc Position
September 2005 - December 2013
Technische Universität München
Position
  • PostDoc Position
Description
  • PostDoc in the working group of Claudia Traidl-Hoffmann
Education
November 2000 - April 2004

Publications

Publications (97)
Article
Full-text available
Intraspecific genetic variation might limit the relevance of environmental factors on plant traits. For example, the interaction between genetics and (a-)biotic factors regulating pollen production are still poorly understood. In this study, we investigated pollen production of 28 birch (Betula pendula Roth) individuals in the years 2019-2021. We s...
Article
Full-text available
Seasonal exposure to birch pollen (BP) is a major cause of pollinosis. The specific role of Toll-like receptor 4 (TLR4) in BP-induced allergic inflammation and the identification of key factors in birch pollen extracts (BPE) initiating this process remain to be explored. This study aimed to examine (i) the importance of TLR4 for dendritic cell (DC)...
Article
Hypersensitivity reactions are overreactions of the immune system clinically seen as allergic and autoimmune diseases. Gell and Coombs originally described four different types of hypersensitivity reactions almost 60 years ago, and their description still applies in large parts. However, some modifications and extensions have been included in origi...
Article
Full-text available
In a recently published data-orientedstudyin March 2021, a relationship between SARS-CoV-2 infection rates and airborne pollen concentrationswas detected [1]. The studyincluded a data compilation of airborne pollen concentrations and SARS-CoV-2 infection numbers across 31 countries from both hemispheres and all inhabited continents from an inter-di...
Article
Full-text available
Pollen exposure weakens the immunity against certain seasonal respiratory viruses by diminishing the antiviral interferon response. Here we investigate whether the same applies to the pandemic severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), which is sensitive to antiviral interferons, if infection waves coincide with high airborne pol...
Article
Full-text available
The mechanisms involved in the induction of allergic sensitization by pollen are not fully understood. Within the last few decades, findings from epidemiological and experimental studies support the notion that allergic sensitization is not only dependent on the genetics of the host and environmental factors, but also on intrinsic features of the a...
Article
Background: Common ragweed has been spreading as a neophyte in Europe. Elevated CO2 levels, a hallmark of global climate change, have been shown to increase ragweed pollen production, but their effects on pollen allergenicity remain to be elucidated. Methods: Ragweed was grown in climate-controlled chambers under normal (380 ppm, control) or eleva...
Article
Background Pollen exposure induces local and systemic allergic immune responses in sensitized individuals, but nonsensitized individuals also are exposed to pollen. The kinetics of symptom expression under natural pollen exposure have never been systematically studied, especially in subjects without allergy. Objective We monitored the humoral immu...
Article
Full-text available
The epithelial cell-derived cytokine milieu has been discussed as a “master switch” in the development of allergic disease. To understand the role of innate immune response in nasal epithelial cells during allergic inflammation, we created and established a fast and minimally invasive method to isolate and culture human nasal epithelial cells from...
Conference Paper
Pollen and fungal spores (aeroallergens) are main causes of respiratory allergies worldwide. So as to more efficiently treat allergic diseases and especially asthma, higher altitude tourism has been suggested, as aeroallergen exposure becomes significantly shorter and lower in such environments. However, it is not thoroughly investigated as to what...
Conference Paper
Thunderstorm asthma has been reported after unusually high co-occurrence of asthma cases with thunderstorm events. To date, no determinative relationship among involved co-factors has been revealed worldwide. Very few times it has been examined within a data-oriented framework and or for the full diversity spectrum of aeroallergens (pollen, fungal...
Conference Paper
Background: To date, high-risk pollen exposure alerts have been provided only via forecasting models and laborious monitoring methods. The aim of this study was to evaluate the ‘real pollen exposure’ to airborne pollen, using automatic, real-time pollen monitoring devices, as well as conventional ones and comparing against symptoms from dedicated c...
Article
Full-text available
Background: Hundreds of plant species release their pollen into the air every year during early spring. During that period, pollen allergic as well as non-allergic patients frequently present to doctors with severe respiratory tract infections. Objective: To assess whether pollen may interfere with antiviral immunity. Methods: We combined data...
Article
Full-text available
Background: Over 100 million people worldwide suffer from birch pollen allergy. Bet v 1 has been identified as the major birch pollen allergen. However, the molecular mechanisms of birch allergic sensitization, including the roles of Bet v 1 and other components of the birch pollen extract, remain incompletely understood. Here, we examined how kno...
Article
Pollen exposure is a major cause of respiratory allergies worldwide. However, it is unclear how everyday exposure is related to symptoms and how allergic patients may be affected spatially and temporally. Hence, we investigated the relationship of pollen, symptoms and immune responses under a controlled regime of ‘high-low-moderate’ pollen exposure...
Conference Paper
Background: Vegetation, flowering phenology and pollen production are influenced by climate change. Little is known about allergen content. The aim of the study was to investigate for relationships between health-related reproductive attributes of plants and various environmental regimes. Methods: All individual plants of different Betula species w...
Article
Allergies are usually referred to as type I hypersensitivity reactions against innocuous environmental antigens, characterized by a Th2/IgE‐dominated inflammation. They can manifest themselves in various organs, such as skin, gastro‐intestinal and respiratory tract, and comprise diseases as diverse as allergic rhinitis and conjunctivitis, bronchial...
Conference Paper
Full-text available
Background: The epithelial cell-derived cytokine milieu has been discussed as a “master switch” in the development of allergic disease. To understand the role of innate immune response in human nasal epithelial cells (HNECs) during allergic inflammation. We established a fast and minimal invasive method to isolate and culture HNECs from clinically...
Article
In contrast to the pollen context, the major allergen Bet v 1 alone is not responsible for Th2 polarization in allergic sensitization. These findings are highly relevant for birch pollen allergy prophylaxis.
Conference Paper
Background: Pollen and fungal spores (aeroallergens) are main causes of respiratory allergies worldwide. However, it is still unclear how everyday exposure to aeroallergens is related to symptoms. Is there therefore a ‘safe’ place or time period that we can ‘switch off’ allergies? To answer this, we investigated the spatiotemporal patterns of airbo...
Conference Paper
Airborne pollen is implicated in respiratory allergy symptoms worldwide. Particularly birch pollen exhibits the direst impact on allergic individuals in boreal and temperate climates. However, the mechanistic effects of airborne pollen abundance on the actual symptoms of allergic individuals have not been clarified and the role of co-factors like l...
Article
Ze339, an herbal extract from Petasites hybridus leaves is effective in treatment of allergic rhinitis by inhibition of a local production of IL-8 and eicosanoid LTB4 in allergen-challenged patients. However, the mechanism of action and anti-inflammatory potential in virally induced exacerbation of the upper airways is unknown. This study investiga...
Article
Atopic dermatitis (AD) is a complex skin disease frequently associated with other diseases of the atopic diathesis. Recent evidence supports the concept that AD can also recognize other comorbidities, such as chronic inflammatory bowel or cardiovascular diseases. These comorbidities might result from chronic cutaneous inflammation or from a common,...
Article
Background: Besides allergens, pollen release bioactive, low molecular weight compounds that modulate and stimulate allergic reactions. Clinical relevance of these substances hasn't been investigated to date. Objective: To elucidate the effect of a non-allergenic, low molecular weight factors from aqueous birch pollen extracts (Bet-APE<3kDa) on...
Article
Full-text available
Pollen allergies have been rapidly increasing over the last decades. Many allergenic proteins and non-allergenic adjuvant compounds of pollen are involved in the plant defense against environmental or microbial stress. The first aim of this study was to analyze and compare the colonizing microbes on allergenic pollen. The second aim was to investig...
Data
Correlation of pollution parameters NO2 and O3 to the Urbanization Index (UI). Ambient NO2 concetration is significantly positive correlated to the Urbanization Index (UI; rho = 0.68, p < 0.0001), whereas ambient O3 concentration is significantly negative correlated to the Urbanization Index (rho = -0.46, p < 0.001). (TIF)
Data
Correlation of bacterial diversity on timothy grass pollen to the content of PALMs and allergens and to the Urbanization Index (UI). Spearman-Correlation of bacterial diversity-indices (Simpson, Shannon) and the absolute number of different fragments (n(tRFs)) analyzed from timothy grass pollen (Phleum pratense, n = 20; 2014) to the produced amount...
Data
Correlation of fungal diversity on birch pollen to the content of PALMs and allergens, to the activity of NADPH oxidase as well as to measured pollution parameters. Spearman-Correlation of fungal diversity-indices (Simpson, Shannon) and the absolute number of different fragments (n(tRFs)) analyzed from birch pollen (Betula pendula, 2014) to air pol...
Data
Correlation of fungal diversity on timothy grass pollen to the content of PALMs and allergens and to the Urbanization Index (UI). Spearman-Correlation of fungal diversity-indices (Simpson, Shannon) and the absolute number of different fragments (n(tRFs)) analyzed from timothy grass pollen (Phleum pratense, 2014) to the produced amount of allergens...
Article
Full-text available
Background: Pollen allergens are delivered to epithelial surfaces of the upper respiratory tract in conjunction with multiple endogenous adjuvants. We previously demonstrated pollen-mediated modulation of cytokine and chemokine production of dendritic cells, contributing to a Th2-dominated micromilieu. As T helper cell differentiation not only dep...
Article
B cells play a central role in IgE-mediated allergies. In damaged airway epithelium they are exposed directly to aeroallergens. We aimed to assess whether direct exposure of B cells to pollen constituents affects allergic sensitization. B cells from murine splenocytes and from blood samples of healthy donors were incubated for 8 days under Th2-like...
Article
Full-text available
The bronchial epithelium plays a key role in providing a protective barrier against many environmental substances of anthropogenic or natural origin which enter the lungs during breathing. Appropriate responses to these agents are critical for regulation of tissue homeostasis, while inappropriate responses may contribute to disease pathogenesis. He...
Article
Background Ragweed (Ambrosia artemisiifolia) is a strong elicitor of allergic airway inflammation with worldwide increasing prevalence. Various components of ragweed pollen are thought to play a role in the development of allergic responses. Aim of the study was to identify critical factors for allergenicity of ragweed pollen in a physiologic model...
Article
Allergien und Autoimmunerkrankungen sind weltweit auf dem Vormarsch. Die Kontrolle von Infektionskrankheiten stellt jedoch auch im postantibiotischen Zeitalter eine Herausforderung dar. Chronische und/oder schwer kontrollierbare Infektionen treten bei Menschen mit Immunschwäche auf, wie z. B. bei HIV-Patienten, hospitalisierten Patienten, die multi...
Article
Allergies and autoimmune diseases are spreading worldwide. Control of infections, on the other hand, remains an issue, even in the post-antibiotic era. Chronic or poorly controlled infections occur in immune compromised individuals such as HIV patients, hospitalized patients exposed to multi-resistant bacteria, or patients on immunosuppressive trea...
Article
Full-text available
Evidence is compelling for a positive correlation between climate change, urbanisation and prevalence of allergic sensitisation and diseases. The reason for this association is not clear to date. Some data point to a pro-allergenic effect of anthropogenic factors on susceptible individuals. To evaluate the impact of urbanisation and climate change...
Article
Mit dieser Ausgabe startet die neue Serie „Das kleine 1 x 1 der Immunologie“. Den Auftakt machen auf der folgenden Doppelseite die dendritischen Zellen. Immunologisch unter die Lupe genommen werden im weiteren Verlauf der Serie aber auch Mastzellen, T-Zellen und B-Zellen, Mediatoren wie Zytokine sowie beteiligte Grenzflächen — angefangen bei Haut,...
Article
Full-text available
The question what makes an allergen an allergen puzzled generations of researchers. Pollen grains of anemophilous plants are the most important allergen carriers in ambient air, and pollinosis is a highly prevalent multi-organ disease in civilized countries. In the past, research on the allergenicity of pollen has mainly focused on elucidating gene...
Article
Water-soluble components from pollen modulate dendritic cell (DC) functions, such as IL-12 secretion and 3'-5'-cyclic adenosine monophosphate (cAMP) signaling and migration, possibly contributing to the establishment of a T(H)2-dominated immune response against pollen. Because these effects could not solely be attributed to the previously identifie...