Stefan G. Pierzynowski

Stefan G. Pierzynowski
Lund University | LU · Department of Biology

Dr. DSc. Professor

About

310
Publications
23,069
Reads
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3,647
Citations
Additional affiliations
January 1998 - December 1998
Langston University
Position
  • visiting prof
August 1985 - present
Lund University
Position
  • Researcher - Professor

Publications

Publications (310)
Article
Full-text available
Alpha-ketoglutarate (AKG) is one of the key metabolites that play a crucial role in cellular energy metabolism. Bariatric surgery is a life-saving procedure, but it carries many gastrointestinal side effects. The present study investigated the beneficial effects of dietary AKG on the structure, integrity, and absorption surface of the small intesti...
Article
Full-text available
Preterm birth is associated with increased risk of complications, specifically with regards to the gastrointestinal tract. These complications mainly include the maldigestion and malabsorption of nutrients resulting from the immaturity of the small intestine. The current study investigated whether pre-digestion of fat in infant formula would affect...
Article
Purpose: Nitrogen (N2) is an indispensable metabolite required for the synthesis of protein. In animals, gut bacteria and, to a certain extent, even hepatocytes, are able to assimilate nitrogen from ammonium (NH4+), which is essentially derived from the amine group (-NH2) and which is at the same time a very toxic metabolite. Initially, NH4+ is co...
Article
Full-text available
Pancreatic enzyme replacement therapy (PERT) and fat predigestion are key in ensuring the optimal growth of patients with cystic fibrosis. Our study attempted to highlight differences between fat predigestion and conventional PERT on body composition of young pigs with exocrine pancreatic insufficiency (EPI). EPI and healthy pigs were fed with high...
Article
Full-text available
The current study was aimed at highlighting the role of blood pancreatic amylase in the regulation of glucose homeostasis and insulin secretion in a porcine model of streptozotocin- (STZ-) induced diabetes and in a rat pancreatic beta-cell line, BRIN-BD11. Blood glucose, plasma insulin, and glucagon levels were measured following a duodenal glucose...
Article
Full-text available
Limitations in efficacy and/or tolerance of currently available urate-lowering therapies (ULTs), such as oral xanthine oxidase inhibitors, uricosurics, and intravenous uricase agents contribute to the development of refractory gout. Renal excretion is the major route of uric acid elimination, but the intestinal tract plays an increasingly recognize...
Preprint
Full-text available
Introduction. Recently, more attention is being paid to the drug-induced liver injury (DILI) as a consequence of the tuberculos is treatment and the need for new medicine is emphasized. The use of isoniazid and rifampicin has a potentiating effect, which increases the risk of substancial liver damage. In turn, systemic accumulation of toxic metabol...
Article
Full-text available
The gut is an efficient barrier which protects against the passage of pathogenic microorganisms and potential harmful macromolecules into the body, in addition to its primary function of nutrient digestion and absorption. Contrary to the restricted macromolecular passage in adulthood, enhanced transfer takes place across the intestines during early...
Article
Full-text available
Obestatin is a gastrointestinal peptide having wide-ranging effects on cell proliferation; however, its mechanism of action remains poorly understood. Thus, the aim of the study was to elucidate the effect of exogenous obestatin on the postnatal structural development of the small intestine. Seven-day-old piglets with an average BW of 1.56 ± 0.23 k...
Article
Full-text available
In the present review, we highlight the possible "extra-immunological" effects of maternal immunoglobulins (Ig) transferred to the blood circulation of offspring, either via the placenta before birth or via the colostrum/milk across the gut after birth in different mammalian species. Using the newborn pig as a model, since they are naturally born a...
Article
Full-text available
The current study is aimed at highlighting the impact of enterally or parenterally applied immunoglobulins (Igs) on polyunsaturated fatty acid (PUFA) absorption in newborn pigs. Piglets were chosen as the appropriate model since they are born agammaglobulinemic and any effects of Ig addition can thus be easily monitored. Twenty-one, new born piglet...
Article
It has been demonstrated in animal studies that prenatal administration of β‐hydroxy‐β‐methylbutyrate (HMB, metabolite of leucine) influences general growth and mechanical endurance of long bones in newborn offspring in sex‐dependent manner. The present experiment was conducted to evaluate the effect of HMB treatment of pregnant sows on bone develo...
Article
Full-text available
Ageing is a complex physiological process characterized by a gradual decline in cell, tissue and organ function. Consequently, ageing is a major risk factor for cardiovascular disease, diabetes and neurodegenerative disorders. Altered nervous system function can lead to behavioural or psychological disorders. Recent research has shown that alpha ke...
Article
Full-text available
Studies have highlighted the existence of two intra-pancreatic axes of communication: one involved in the regulation of enzyme production by insulin—the insular–acinar axis; and another involved in the regulation of insulin release by pancreatic enzymes—the acini–insular axis. Previous studies by our laboratory show that pancreatic enzymes can affe...
Article
Full-text available
The purpose of this research is to explore the link between plasma amylase and insulin levels in growing pigs. Blood was obtained from piglets ranging in age from preterm (8 days to full gestation period), up to postnatal day 90 (2 months post-weaning) that underwent either duodenal-jejunal bariatric interposition surgery or a sham-operation. Plasm...
Article
Full-text available
The studies presented were designed to highlight the impact of pancreatic enzymes on glycemic control and insulin response. Blood glucose and plasma insulin levels were monitored after intravenous, oral or direct gut glucose tolerance tests (GTT) in 6 pigs with an intact gastrointestinal tract and in 12 pigs following duodenal-jejunal bypass (DJB)...
Article
Full-text available
Introduction. Kidney stone formation may be a result of increased urinary oxalate supersaturation. Material and Methods. Eighteen pigs were randomly divided into: Control group, where standard cereal-based feed was supplemented with 4% HP only, Prevention group, where treatment with OxDc slurry started at the end of the adaptation period when pigs...
Article
Full-text available
Neurological disorders are among the main clinical problems affecting preterm children and often result in the development of communication and learning disabilities later in life. Several factors are of importance for brain development, however the role of immunoglobulins (passive immunity transfer) has not yet been investigated. Piglets are born...
Article
Full-text available
An elevated level of serum uric acid - hyperuricemia, is strongly associated with the development of gout and chronic kidney disease (CKD) which is often accompanied by a significantly reduced glomerular filtration rate (GFR). In the present study, we investigated the extra-renal elimination of uric acid via the intestine in a healthy pig model and...
Data
Oral uricase eliminates blood uric acid in the hyperuricemic pig model. Study protocol. Original study protocol. (DOCX)
Article
Full-text available
Aim of the study. In this study, we sought to create a model of reversible hyperoxaluria in pigs by feeding with hydroxyproline (HP). Materials and methods. The experiment included 12 pigs divided into 2 groups (n = 6). The pigs were fed twice a day. At the beginning of the experiment, in the adaptation period, all pigs were given standard feed. In...
Article
Full-text available
Background A growing body of literature on Roux-en-Y gastric bypass surgery (RYGB) has generated inconclusive results on the mechanism underlying the beneficial effects on weight loss and glycaemia, partially due to the problems of designing clinical studies with the appropriate controls. Moreover, RYGB is only performed in obese individuals, in wh...
Data
Insulin and glucose levels during the IVGTT. (XLSX)
Data
Bile acid levels in blood plasma, bile and faeces. (XLSX)
Data
Alterations in fasted metabolite levels after RYGB and sham-surgery. (PDF)
Article
Gastric bypass surgery results in remission of type 2 diabetes in the majority of patients. The incretin hormones glucagon-like peptide 1 (GLP-1) and glucose-dependent insulinotropic polypeptide (GIP) have been implicated in the observed remission. Most knowledge so far has been generated in obese subjects. To isolate the surgical effects of gastri...
Article
Synthetic glucocorticoids (GCs) are widely used in the variety of dosages for treatment of premature infants with chronic lung disease, respiratory distress syndrome, allergies, asthma, and other inflammatory and autoimmune conditions. Yet, adverse effects such as glucocorticoid-induced osteoporosis and growth retardation are recognized. Conversely...
Article
Full-text available
The images for Figs 6, 7 and 8 are incorrectly switched. The image that appears as Fig 6 should be Fig 7, the image that appears as Fig 7 should be Fig 8, and the image that appears as Fig 8 should be Fig 6. The figure captions appear in the correct order. © 2017 Arévalo Sureda et al. This is an open access article distributed under the terms of th...
Article
Preterm human neonates, contrary to preterm piglets, obtain immunoglobulins from their mothers via the placenta during intrauterine development. However, one should note that the majority of trans-placental transfer of immunoglobulins in humans takes place during the last trimester of pregnancy. It is also known that the feeding of limited amounts...
Article
Full-text available
Background The intestinal barrier is immature in newborn mammals allowing for transfer of bioactive macromolecules, e.g. protecting antibodies, from mother’s milk to the blood circulation and in neonatal rodents lasts until weaning. This passage involves the neonatal-Fc-receptor (FcRn) binding IgG in the proximal and highly endocytic vacuolated ent...
Data
The specificity of the anti-Blimp-1 antibody was verified by neutralization with the Blimp-1 peptide control. Immunohistochemistry with and without blocking with the immunizing 14-aa Blimp-1 peptide (antibody: blocking peptide ratio; 1:5) in the distal part of the SI in suckling 14 days old and post-weaning 28 days old rats. In contrast to anti-Bli...
Data
Replacement of vacuolated epithelium by non-vacuolated epithelium in the distal part of the SI during postnatal development in rats. H & E stained representative histological sections (200X) showing the structural changes in the proximal and distal parts of the SI during postnatal development in 7, 14, 21, 28 and 35 days (d) old rats. (TIF)
Data
Correlation between FcRn expression and plasma IgG during postnatal development in rats. Changes of plasma IgG (mg/ml, mean ± SD) and appearance of adult-type cells (% FcRnneg of total villi cells, mean ± SD) in the proximal SI during postnatal development in 7, 14, 21, 28 and 35 day old rats. Note the inverse correlation with a coefficient of r =...
Data
Replacement of FcRnpos epithelium by FcRnneg epithelium in the SI during postnatal development in rats. Immunohistochemistry of FcRn (200X) in representative histological sections from the proximal and distal SI during postnatal development in 7, 14, 21, 28 and 35 days (d) old rats. (TIF)
Data
Changes in FcRn expression in the distal part of the SI of rats due to precociously induced maturation. Immunohistochemistry of FcRn (200X) in representative histological sections from the distal SI in 17 days old rats treated with PHA or protease at 14–16 days of age to induce precocious gut maturation, compared to control rats. Note the increased...
Article
Alpha-ketoglutarate (AKG) is an important intermediate in Krebs cycle which bridges the metabolism of amino acids and carbohydrates. Its effects as a dietary supplement on cold tolerance were studied in D. melanogaster Canton S. Two-day-old adult flies fed at larval and adult stages with AKG at moderate concentrations (5-10 mM) recovered faster fro...
Article
Full-text available
'Heat' diarrhea in foals is an onerous but not life-threatening ailment, which indicates that it may be of osmotic origin. This was confirmed by a successful attempt, presented in this paper, to alleviate the severity and duration of foal heat diarrhea with the use of a typical absorbent, kaolin clay, as a feed additive, usually applied in feed pro...
Article
Full-text available
We have previously shown that the presence of pancreatic enzymes in the gut lumen of exocrine pancreatic insufficient pigs influences blood glucose and insulin levels during an intravenous glucose tolerance test (IVGTT). The present study aims to highlight the effects of orally applied pancreatic-like enzymes on blood glucose and plasma insulin lev...
Article
Full-text available
Objective This study investigated the effect of alpha-ketoglutarate sodium salt (AKG) and ipriflavone (IP) treatment on the mineralization of the tibia in male rats during the development and after the establishment of osteopenia. Design One hundred and twenty eight male rats were randomly selected and submitted to either sham-operation (SHO) or or...
Article
Full-text available
It is well known that during the suckling period pancreatic secretion is low. The aim of the study was to investigate the effects of dietary supplementation with pancreatic-like enzymes of microbial origin (PLEM) and SiO2, as an antibacterial factor, on the performance of piglets fed creep feed during weaning and thereafter. The experiment was perf...
Article
The effect of exocrine pancreatic function on the glucose-mediated insulin response and glucose utilization were studied in an exocrine pancreas-insufficient (EPI) pig model. Five 10-week-old EPI pigs after pancreatic duct ligation and 6 age-matched, non-operated control pigs were used in the study. Blood glucose, plasma insulin and C-peptide conce...
Article
Treatment with pancreatic or pancreatic-like microbial enzymes during the suckling period has positive effects on the gut, resulting in a better fat absorption and improved barrier function in pigs. The present study investigated the effects of pancreaticlike enzyme treatment during the pre- or postweaning period on pig growth and nitrogen utilizat...
Article
Gastrectomy (Gx) leads to osteopenia/osteoporosis in humans and animals. However, little is known about the influence of Gx on the cartilage in this regard. Recent studies have demonstrated a protective effect of 2-oxoglutaric acid (2-Ox) on bone and cartilage. Hence, the purpose of this study was to investigate whether 2-Ox can mitigate eventual G...
Article
Background Patients with CF and EPI have difficulty managing weight requiring liquid supplements and tube feeds. Administration of PERTs with enteral feeding is challenging, as enzyme availability is mismatched with formula, negatively affecting fat digestion, absorption and leading to fatty acid deficiencies. EFIC contains an immobilized lipase wi...
Article
Full-text available
Background/objectives: Aging is associated with many physiological alterations such as changes in metabolism, food intake and brain dysfunction. Possible ways to correct age-related brain dysfunction using dietary treatments still remains undeveloped. The aim of our research was to investigate whether long-term dietary treatment with 2-oxoglutarat...
Article
Full-text available
It is postulated that exocrine pancreatic insufficiency (EPI) can evoke neurological disorders. In the present study pancreatic-like enzymes of microbial origin (PLEM) were examined as a functional food component, with the goal of improving cognitive function and brain structure in a pig model of EPI. EPI in the present study induced alterations in...
Article
Full-text available
Postnatal gut maturation in neonatal mammals, either at natural weaning or after precocious inducement, is coinciding with enhanced enzymes production by exocrine pancreas. Since the involvement of enzymes in gut functional maturation was overlooked, the present study aimed to investigate the role of enzymes in gut functional maturation using neona...
Article
Purpose: Plasma levels of pancreatic and intestinal enzymes were measured after pancreatic duct ligation (PDL) to monitor pancreatic exocrine insufficiency (PEI) in a model using young pigs. Material/methods: Five, 6 week-old pigs (10.9±0.2kg), underwent PDL while age-matched, un-operated pigs were used as controls. Plasma levels of immunoreacti...
Article
Full-text available
Background: In most patients gastric bypass (GBP) causes remission of type 2 diabetes. It is established that plasma levels of gut hormones are affected by GBP, but it is not well understood how the enteroendocrine cells producing these gut hormones are affected by GBP.