Stanislav Sukhikh

Stanislav Sukhikh
Immanuel Kant Baltic Federal University | IKSUR · Institute of Living Systems

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66
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Introduction

Publications

Publications (66)
Article
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Hydrothermal liquefaction (HTL) of sewage sludge is considered in the article as an analogue of the natural processes of oil formation (catagenesis). A comparison of the physicochemical composition of primary and secondary sludge with type II kerogen (natural precursor of oil) showed their similarity. Both types of sludge have a slightly higher lev...
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Miscanthus is a perennial wild plant that is vital for the production of paper and roofing, as well as horticulture and the development of new high-yielding crops in temperate climates. Chromosome-level assembly of the ancient tetraploid genome of miscanthus chromosomes is reported to provide resources that can link its chromosomes to related diplo...
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Lignocellulose, the main component of plant cell walls, comprises polyaromatic lignin and fermentable materials, cellulose and hemicellulose. It is a plentiful and renewable feedstock for chemicals and energy. It can serve as a raw material for the production of various value-added products, including cellulase and xylanase. Cellulase is essential...
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The pulp and paper industry leads to the formation of significant amounts of bark and wood waste (BWW), which is mostly dumped, causing negative climate and environmental impacts. This article presents an overview of methods for recycling BWW, as well as the results of assessing the resource potential of old bark waste based on physicochemical and...
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Methods for purifying, detecting, and characterizing protein concentrate, carbohydrates, lipids, and neutral fats from the microalgae were developed as a result of research. Microalgae were collected from natural sources (water, sand, soil of the Kaliningrad region, Russia). Microalgae were identified based on morphology and polymerase chain reacti...
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The prohibition of antibiotics has led to extensive research and use of phytogenic feed additives. James Barrie Kirkpatrick described four subspecies of eucalyptus (family Myrtaceae), including Eucalýptus globulus, in 1974. The maximum concentrations of quercetin-3D-glycoside (1703.30 g/mL), astragalin (1737.82 g/mL), chlorogenic acid (342.14 g/mL)...
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The cell wall of brown algae contains alginate as a major constituent. This anionic polymer is a composite of β-D-mannuronate (M) and α-L-guluronate (G). Alginate can be degraded into oligosaccharides; both the polymer and its products exhibit antioxidative, antimicrobial, and immunomodulatory activities and, hence, find many commercial application...
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The excess biomass of drifting algae and their casting to the Baltic Sea coast imposes a significant environmental burden. The analysis of beach-cast algae showed that the dominant species are macroalgae Ulva sp., Furcellaria lumbricalis, Cladophora sp., and Polysiphonia fucoides. The biomass of Furcellaria and Polysiphonia algae, containing 25.6%...
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Maintaining quality of life with an increase in life expectancy is considered one of the global problems of our time. This review explores the possibility of using natural plant compounds with antioxidant, anti-inflammatory, anti-glycation, and anti-neurodegenerative properties to slow down the onset of age-related changes. Age-related changes such...
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The beneficial properties of algae make them perfect functional ingredients for food products. Algae have a high energy value and are a source of biologically active substances, proteins, fats, carbohydrates, vitamins, and macro- and microelements. They are also rich in polyunsaturated fatty acids, proteins, mycosporine-like amino acids, polysaccha...
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At the moment, macroalgae blooms in sea waters, the rotting of which causes greenhouse gas emissions and contributes to the formation of a negative ecological and economic situation in coastal zones, which has become a serious problem. Fuel production through hydrothermal liquefaction (HTL) of macroalgae and marine debris is a promising solution to...
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The lignocellulosic perennial crop miscanthus, especially Miscanthus × giganteus, is particularly interesting for bioenergy production as it combines high biomass production with low environmental impact. However, there are several varieties that pose a hazard due to susceptibility to disease. This review contains links showing genotype and ecologi...
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This paper aimed to study the biodegradation of collagen-containing waste (pork skin) induced by collagenase and Neutrase 1.5 MG enzymes and compare the amino acid, peptide, and carbohydrate composition of hydrolysis products. It was found that the degree of biodegradation of collagen-containing raw materials (pork skin) reached 78% when using an e...
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Determination of the biocompatibility of microorganisms isolated from natural sources (Kemerovo Oblast—Kuzbass) resulted in the creation of three microbial consortia based on the isolated strains: consortium I (Bacillus pumilus, Pediococcus damnosus, and Pediococcus pentosaceus), consortium II (Acetobacter aceti, Pseudomonas chlororaphis, and Strep...
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Enzymatic hydrolysis of cellulose-containing raw materials, using Aspergillus niger, were studied. Filter paper, secondary cellulose-containing or starch-containing raw materials, miscanthus cellulose after alkaline or acid pretreatment, and wood chip cellulose, were used as substrates. The study focused on a wild A. niger strain, treated, or not (...
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Extremophilic microorganisms attract researchers by their unique characteristics, primarily antagonistic ones, acquired in the process of survival in extreme natural conditions. The antimicrobial potential of the metabolites of these microorganisms is quite broad, from the food industry to therapeutic drugs. Microbial mats of Lake Baikal are a sour...
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Natural habitats, including extreme ones, are potential sources of new antimicrobial compound producers, such as bacteriocins and enzymes, capable of degrading the matrix polysaccharides of bacterial biofilms. This study aimed to investigate biodiversity and evaluate the antibacterial potential of psychrophilic and psychrotrophic microbial communit...
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This study reveals that fossil shungite samples exhibit antioxidant activity, can reduce oxidized components, and bind to free radicals. A sample of Sh20 (size fraction—20 µm) (1.30 mg equivalents of ascorbic acid/g of shungite; 3.46 mg equivalents of trolox/g of shungite; 0.99 mg equivalents of quercetin/g of shungite) had the maximal activity acc...
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Our study focused on investigating the possibilities of controlling the accumulation of carbohydrates in certain microalgae species (Arthrospira platensis Gomont, Chlorella vulgaris Beijer, and Dunaliella salina Teod) to determine their potential in biofuel production (biohydrogen). It was found that after the introduction of carbohydrates (0.05 g⋅...
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The medicinal plant Dactylorhiza maculata (L.) Soó (family Orchidaceae) is used to treat gastritis, colic, gastrointestinal tract, and bladder diseases. This study aimed to investigate the properties and characteristics of the in vitro Dactylorhiza maculata extract. The recommended parameters for producing Dactylorhiza maculata extract were determi...
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The results of the studies have shown that to obtain an extract of a complex of biologically active substances of Cotinus coggygria, ethyl alcohol (mass fraction of alcohol 70%) with a hydromodule of 1:5 should be used, and the extraction should be carried out for 60 min at a temperature of 60 °C. The investigated plant extracts with the complex of...
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Introduction. Cellulose-containing parts of herbs are an excellent source of alternative energy and can be used to produce biological ethanol. The present research aims at improving this fundamental and promising area of biotechnology. It introduces a new consortium of microorganisms that can saccharify while fermenting the substrate. Study objects...
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Bacteriocins are of great interest as potential antimicrobial agents against various types of bacteria, fungi, and viruses. Isolates of microorganisms derived from natural sources were used in the current study, including lactic acid bacteria and other antagonistic microorganisms. The species of the microorganisms were determined using 16S rDNA and...
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Microalgae are known to be rich in protein. In this study, we aim to investigate methods of producing and purifying proteins of 98 microalgae including Chlorella vulgaris, Arthrospira platensis, Nostoc sp., Dunaliella salina, and Pleurochrysis carterae (Baltic Sea). Therefore, we studied their amino acid composition and developed a two-stage protei...
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The cultivation of plants in the form of callus cultures constitutes a renewable source of secondary plant metabolites. The conditions for the cultivation of callus cultures affect the yield of target compounds. Callus cultures of Hyssopus officinalis were chosen for study. Nutrient media of various compositions were used for Hyssopus officinalis c...
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Osteoarthritis (OA) is a degenerative joint disease and an important cause of incapacitation. There is a lack of drugs and effective treatments that stop or slow the OA progression. Modern pharmacological treatments, such as analgesics, have analgesic effects but do not affect the course of OA. Long-term use of these drugs can lead to serious side...
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The kinetics of L-phenylalanine ammonia-lyase (PAL) penetration into the monolayer of liver cells after its release from capsules was studied. The studies showed the absence of the effect of the capsule shell based on plant hydrocolloids on the absorption of L-phenylalanine ammonia-lyase in systems simulating the liver surface. After 120 min of inc...
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This work aims to study the qualitative composition of biologically active substance (BAS) extracts in vitro callus, cell suspension, and root cultures of the medicinal plant Rhaponticum carthamoides. The research methodology is based on high-performance liquid chromatography, and 1H nuclear magnetic resonance (NMR) spectra, to study the qualitativ...
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The article presents the characteristics of the chemical composition of the biomass of genus Miscanthus plants and the features of its use for the production of carbohydrate-containing substrates for biotechnology. It is shown that the Chinese mycanthus “Ferner Osten” is not characterized by a high content of lipidic and protein compounds. The herb...
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The results of the qualitative composition analysis of the dried biomass extracts of in vitro callus, cell suspension, and root cultures show that the main biologically active substances (BAS) in the medicinal plant, Rhodiola rosea, are 6-C-(1-(4-hydroxyphenyl)ethyl)aromadendrin (25 mg, yield 0.21%), 2-(3,7-dihydroxy-2-(2-hydroxypropan-2-yl)-2,3-di...
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Introduction. Extreme psychrophiles still remain largely understudied. However, they can serve as biological components in drug production, as well as increase the shelf life of food products in food industry. Therefore, the properties of psychrophilic microorganisms are a relevant study object. The present research objective was to isolate new psy...
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Aim. To obtain a purified IgG preparation from the plasma of multiparous women and to evaluate its functional activity towards HLA-DR and HLA-G molecules. Materials and Methods. IgG preparation was prepared by means of affinity chromatography using the DEAE Affi-Gel Blue system. The purity of the isolated preparation was assessed by immunoelectroph...
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Microorganisms isolated from natural sources that have antagonistic properties are the objects of the research in this paper. The main objective of the study was to screen and characterize the antagonistic properties of microorganisms isolated from natural sources in connection with the creation of new pharmaceutical substances. Methods of cultivat...
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This paper studies modern methods of producing and using callus, suspension cells and root cultures of medicinal plants in vitro. A new solution for natural product production is the use of an alternative source of renewable, environmentally friendly raw materials: callus, suspension and root cultures of higher plants in vitro. The possibility of u...
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Microalgae are rich in nutrients and biologically active substances such as proteins, carbohydrates, lipids, vitamins, pigments, phycobiliproteins, among others. The lipid composition of the microalgae Chlorella vulgaris, Arthrospira platensis, and Dunaliella salina was screened for the first time. The proposed method for purifying the lipid comple...
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Spinel ferrite magnetic nanoparticles have attracted considerable attention because of their high and flexible magnetic properties and biocompatibility. In this work, a set of magnetic nanoparticles of cobalt ferrite doped with zinc was synthesized via the eco-friendly sol-gel auto-combustion method. Obtained particles displayed a room-temperature...
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The development of new effective anti-coronavirus drugs and therapies is important, but it requires significant human, financial and, most importantly, time expenditures. The current pandemic is neither the first nor the last. Humanity has already accumulated considerable survival experience. We cannot do without prevention and epidemiological prot...
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Introduction. One of the urgent problems of medicine and biology is the use of plant objects as industrial producers of target metabolites in vitro. In vitro cells can be used as pharmaceutical preparations. Study objects and methods. The present research featured medicinal plants that grow in the Siberian Federal district and are a popular source...
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Introduction. Increased resistance of microorganisms to traditional antibiotics has created a practical need for isolating and synthesizing new antibiotics. We aimed to study the antibiotic activity and resistance of bacteriocins produced by lactic acid bacteria and other microorganisms. Study objects and methods. We studied the isolates of the fol...
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Background. Higher fatty alcohols are a valuable raw material for pharmacology and can serve as an adaptogen in crop production, reducing post-stress effects in the cultivation of crops. Policosanols are linear saturated aliphatic alcohols with carbon atoms ranging from 20–24 to 32–34. Cereal germ contains some of the policosanols and can serve as...
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This paper summarizes information about the division of bacteriocins into classes (Gram-negative bacteria, Gram-positive bacteria, and archaea). Methods for producing bacteriocins have been studied. It is known that bacteriocins, most successfully used today are products of secondary metabolism of lactic acid bacteria. It is established that the ma...
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Osteoarthritis is one of the most common diseases of the connective tissue of the elderly. It was found that most epidemiological studies used the Kellgren and Lawrence system for classification of osteoarthritis, which indicates one of the 5 degrees (0–4) of osteoarthritis in various joints according to the radiographic atlas. It has been proven t...
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Microalgae are a group of autotrophic microorganisms that live in marine, freshwater and soil ecosystems and produce organic substances in the process of photosynthesis. Due to their high metabolic flexibility, adaptation to various cultivation conditions as well as the possibility of rapid growth, the number of studies on their use as a source of...
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The study objects were nanoparticles of zinc oxide and titanium dioxide. The aim was to investigate the dependence of antimicrobial activity of solutions with nanosize powders of different particle sizes and concentrations. It has been established that nanoparticles of zinc oxide (size 2−4 nm and 5−7 nm) at a suspension concentration varying from 0...
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Milk and dairy products are staple foods in the diet of all social groups. Plant additives are of multifunctional use in the dairy industry. Wild plants are a source of vitamins, minerals, and other biologically active substances. Due to these compounds, they improve digestion, cardiovascular activity, and emotional state. This review describes the...
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Aim . To study the chromatographic efficiency of modified Sepharose CL-4B sorbents for separation, purification, and preparation of major components from Hedysarum theinum Krasnob. and Hedysarum neglectum Ledeb. root extracts. Materials and Methods . Sorption capacity of the native and modified sepharose was evaluated in both isocratic and gradient...
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During the cultivation and harvesting of fruit and vegetables, a large number of microorganisms accumulate on their surface. Their active and excessive reproduction leads to spoilage of products. The purpose of the study was to assess the effect of combining various packaging technologies with different biopreservatives on the stability of physicoc...
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The presence of toxicological effects and the effect of increasing endurance from the consumption of processed products of pine nut shells (carbohydrate and mineral complex) have been studied. To study the possible toxicological effects of the carbohydrate-mineral complex in vivo, the effect of feed mixtures with the addition of a carbohydrate-mine...
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The existing methods of disinfection of containers for fermented milk products proved to be ineffective. The present research featured an antimicrobial preparation based on silver, copper, and zinc nanoparticles as a disinfectant solution. The authors studied the properties of the antimicrobial in order to prove its effectiveness in processing cont...
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Functional products are currently attracting a lot of research interest. Modern people’s diet does not satisfy their need for nutrients, vitamins and minerals, and functional products can make it more balanced. In particular, our diet is lacking in protein. This paper discusses the prospects for enriching dairy products with plant protein derived f...
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Hyperammonemia is a metabolic disorder, which is caused as a result of high levels of ammonia present in the blood. Hyperammonemia is related to severe liver diseases, primarily to cirrhosis in 90% of cases. Non-cirrhotic causes should be considered in the remaining 10%. The article describes various causes and clinical features of hyperammonemia r...
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The protein concentrate was prepared from oat bran pre-treated with amyloglucosidase. The influence of amyloglucosidase on the efficiency of protein extraction and the functional properties of the resulting protein products were studied in comparison to the protein concentrate obtained by the conventional alkaline method. Samples, pre-treated with...
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In the last decade there has been a very active study of large microbial community in the human gut. The concept appeared explaining the development and persistence of symptoms in irritable bowel syndrome due to disturbances of the intestinal microbial landscape. Manipulation of the intestinal microflora represents a new strategy for treatment of t...