Srikant Sahu

• L V Prasad Eye Institute

Purpose To study the techniques, clinical outcomes, and complications of autologous Tenon’s patch grafting in patients with non-infectious corneal melt. Methods This retrospective analysis included 15 eyes from 15 patients with non-infective corneal melt who underwent autologous Tenon’s patch grafting between January 2021 and December 2023 at our...

In this case series, we will describe sterile keratitis in the early postoperative period after hypotonic collagen cross-linking (CXL) in two pediatric cases. This is a retrospective case series of two eyes of two children who developed inflammatory keratitis with central corneal haze after uneventful epithelial-off hypotonic CXL. After ensuring mi...

Purpose To compare the visual outcomes, postoperative complications, and graft asymmetry between precut and manually dissected donor tissues for Descemet stripping automated endothelial keratoplasty (DSAEK) and Descemet stripping endothelial keratoplasty (DSEK) procedures, respectively. Methods Seventy eyes of 70 patients undergoing DSEK/DSAEK at...

Purpose Corneal biopsy helps in diagnosing deep-seated or recalcitrant lesions of microbial keratitis (MK). We aim to analyze its role in managing these challenging cases. Methods This is a retrospective review of 22 cases of corneal biopsy at our institute from January 2010 to December 2021. Data were retrospectively collected using the electroni...

Purpose To describe the outcomes of eyes with calcium carbide (CaC 2 )-related thermo-chemical injury. Methods This study included 28 eyes of 23 patients who presented with calcium carbide-related ocular burns. Only patients with more than three months of follow-up were included. Group A included 16 eyes with Dua’s Grade I–III burns, while Group B...

PURPOSE: To observe the trends of various types of keratoplasties in different etiologies over a period of 10 years (2011-2020) in a tertiary eye care center of eastern India. METHODS: A retrospective review of patients undergoing keratoplasties from 2011 to 2020 was done in a tertiary eyecare hospital situated in eastern part of India. Apart from...

Purpose: This study was performed to determine the demographic profile and clinical characteristics in patients with Fuchs' endothelial corneal dystrophy (FECD) reporting to a tertiary eye care center in India. It is a retrospective, single-center, observational study. Methods: The study included 280 patients (559 eyes) diagnosed with FECD prese...

Objective: The aim of this study was to describe the clinical features and management of uveitis associated with microsporidial keratoconjunctivitis (MKC). Methods: The medical records of clinically diagnosed or microbiologically proven patients with MKC between July 2016 and August 2021 were reviewed. Patients with documented evidence of kerati...

Purpose: To evaluate various factors affecting the integrity of human donor corneal epithelium. Methods: Donor corneal buttons were evaluated for epithelial defect (ED) and exposure. The slit-lamp photographs were taken on day 01, and the data such as age and gender of the donor, cause of death, refrigeration of cadavers, death-to-preservation...

Purpose: : To determine the presence of SARS-CoV-2 virus in the tear secretion of conjunctivitis patients during the COVID-19 pandemic. Methods: This observational, cross-sectional study was conducted in clinically diagnosed patients with conjunctivitis attending the outpatient services of our institute from July 2021 to December 2021. The tear...

ABSTRACT PURPOSE: To evaluate various factors affecting the integrity of donor corneal epithelium. METHODS: Donor corneal buttons were evaluated for epithelial defect (ED) and exposure. The slit-lamp photographs were taken on day-1, and the data such as age and gender of the donor, cause of death, refrigeration of cadavers, death-to-preservation...

OBJECTIVE: To evaluate the role of McCarey-Kaufman (MK) medium in maintaining the integrity of donor corneal epithelium. METHODS: Nineteen corneal buttons were harvested and stored in MK media at 2-8C for 4 days. Serial photographs were done every day till the 3rd day, and images were then analyzed with ImageJ software (LOCI, University of Wisco...

Objective To compare the clinical presentation, risk factors, causative organisms, and final outcome of early- and late- onset microbial keratitis following Descemet's Stripping Endothelial Keratoplasty (DSEK). Materials And Methods In a retrospective, comparative, case series, twenty-three (10 early-onset keratitis, 13 late-onset keratitis) cases...

PurposeTo study long-term visual and refractive outcomes and complications in eyes with anterior chamber intraocular lens (ACIOL) implantation.Methods Data of patients who underwent primary and secondary ACIOL implantation at L V Prasad Eye Institute, Bhubaneswar between 2011 and 2020 was collected, including details of post-operative visits. For a...

Purpose: To analyze the early changes in host and donor lenticule thickness after Descemet Stripping Endothelial Keratoplasty (DSEK). Methods: DSEK was performed on 32 eyes of 31 patients. Pre- and post- operative slit lamp examination and anterior segment Optical Coherence Tomography (OCT) was done on day-1, day-7, 1 month, and 3 months. Resul...

Background and objective To describe the clinical features, diagnosis and management of immune stromal keratitis/interstitial keratitis (IK) associated with microsporidial epithelial keratitis. Methods Between October 2020 and January 2021, medical records of IK patients microbiologically proven as microsporidia from samples collected from corneal...

Purpose: To compare the endothelial quality of corneas obtained from pseudophakic donors with age-matched phakic controls. Methods: Retrospective analysis of 100 corneas each from pseudophakic and phakic eyes with donor age ≥60 years in both the groups was performed. The endothelial cell density, coefficient of variation, and percentage of hexag...

Ocular microsporidiosis comprises two entirely different spectra of disease as keratoconjunctivitis and stromal keratitis. Microsporidial keratoconjunctivitis (MKC) has been increasingly reported in the past two decades, probably due to raised awareness, simpler diagnostic procedures, and a better understanding of the clinical presentation. It is c...

Purpose To study long term visual and refractive outcomes and complications in eyes with anterior chamber intraocular lens (ACIOL) implantation Methods Data of patients who underwent primary and secondary ACIOL implantation at L V Prasad Eye Institute, Bhubaneswar between 2011-2020 was collected, including details of post-operative visits. For anal...

Purpose: : To characterize the sequelae of microsporidia keratoconjunctivitis (MKC) and outline its management. Methods: Retrospective analysis of microbiologically proven MKC returned with persistent disease between January 2015 and December 2019 was done. Demographics, clinical features, management, and outcome were analyzed. Results: Sixtee...

Purpose: The aim of this work was to study demography, clinical profile, laboratory diagnosis, and management of Pythium keratitis at a tertiary eye care center in Eastern India. Methods: Eighteen patients with culture-positive Pythium keratitis managed at our center between January 2016 and December 2018 were included in this retrospective stud...

Background and objective: To describe the clinical course and propose a morphological classification scheme of microsporidial keratoconjunctivitis. Methods: This is a prospective study at L V Prasad Eye Institute, Odisha, between August-January (2017-19). Patients of any age or sex, microbiologically proven as microsporidial keratoconjunctivitis we...

Purpose To study the type of bacteria and their antibiotic susceptibility pattern from corneal scrapings of patients with infectious keratitis presenting with concurrent nasolacrimal duct obstruction. Methods A retrospective analysis was carried out of patients with microbial keratitis and co-existing nasolacrimal duct obstruction during a 5-year...

Purpose: To compare the clinical profiles and outcomes of patients with Acanthamoeba keratitis with or without mixed bacterial infection. Methods: A retrospective analysis of microbiologically confirmed AK cases presenting to a tertiary care center over a 9-year period was conducted. Fourteen eyes in the coinfected group (group 1) and 24 eyes in...

Microsporidia are obligate spore-forming microorganisms with strong resemblance to fungi and can affect almost every organ system in immunocompetent or immunocompromised individuals. Mixed infections are also reported in immunocompromised hosts. Microsporidial spores show marked morphological variations and the small and slender forms can resemble...

Background Microsporidial stromal keratitis poses several diagnostic challenges. Patients may present with corneal ulceration, marked stromal thinning, or even as a quite corneal scar. The presentation of microsporidial stromal keratitis commonly mimics viral keratitis. Microbiology scrapings are usually helpful; however, scraping and culture-negat...

Purpose: To report the clinical and microbiological profile of keratitis caused by Corynebacterium spp. Methods: The medical and microbiology records of 22 patients, who had presented at the L V Prasad Eye Institute, Bhubaneswar, between June 2009 and December 2012, and whose corneal scrapings had yielded significant growth of Corynebacterium sp...

To determine the causes of culture negativity and compare clinical outcomes in patients with culture-negative (CN) keratitis versus culture-positive (CP) keratitis. Medical and microbiology records of 60 patients, who presented at the L V Prasad Eye Institute, Bhubaneswar, between January 2009 and December 2010, and who were clinically diagnosed to...

To report the molecular and microbiological diagnosis and clinical profile of 13 patients with Pythium insidiosum keratitis. Phase 1 of the study consisted of DNA sequencing of the ITS region of the rDNA of 162 stocked morphologically unidentified nonconsecutive fungal isolates from corneal scraping of patients with keratitis (2010-2012). Blast and...

To compare the efficacy of topical 1% voriconazole vs 5% natamycin for the treatment of fungal keratitis. In a prospective, double-masked, randomised, controlled, registered clinical trial, 118 patients with fungal keratitis were treated using identical dosage schedule with either voriconazole (58) or natamycin (60) as inpatients for 7 days and fol...

Purpose: To determine whether an intracameral injection of cefuroxime sodium at the conclusion of cataract surgery lowers the incidence of acute-onset postoperative endophthalmitis. Setting: Tertiary eye care center, Bhubaneswar, India. Design: Prospective comparative interventional cohort study. Methods: Under a uniform protocol, cataract s...

Purpose: The purpose of this study is to analyze the donor and tissue profile of a community eye bank in Eastern India. Materials and Methods: Eye bank records were analyzed for the period July 2007-June 2011. Variables analyzed included donor demographics (age, gender, and ethnicity), donor cause of death, consent for recovery, death-to-preservat...

The aim of the study was to report the clinical and microbiological profiles of Fusarium keratitis. In this single-centre, retrospective, non-comparative case series, 47 laboratory-confirmed cases of keratitis caused by Fusarium species treated at the L V Prasad Eye Institute, Bhubaneswar, India, between November 2006 and October 2009, were reviewe...

Purpose To evaluate the efficacy of corneal debridement in the treatment of clinically diagnosed cases of microsporidial keratoconjunctivitis. Design Prospective, double-masked, randomized, clinical trial. Methods Patients with clinical features, such as multifocal, coarse, raised, punctate, round to oval epithelial lesions in the cornea in slit-...

To report the clinical and microbiological characteristics and treatment outcome of bacterial keratitis at a tertiary eye care center in eastern India. Retrospective analysis of medical and microbiology records. All patients had undergone complete clinical and microbiological evaluation for microbial keratitis. Patients with laboratory-proven bacte...

A 44-year-old man presented with severe pain, redness, watering and photophobia for 10 days in the right eye without any history of trauma. Diagnosis of herpes simplex disciform keratitis was made and he was prescribed topical steroids. The patient showed clinical worsening and presented with ring infiltrate, diffuse stromal oedema and radial kerat...

The object of this case is to report the clinical findings, microbiological findings and management of a case of fungal scleritis following cataract surgery, which mimicked surgically induced necrotizing scleritis. A 72-year-old Asian (Indian) man presented with scleritis following cataract surgery at another facility. He had been treated elsewhere...

Smear and culture tests of corneal scrapings from a patient with a ring infiltrate confirmed significant growth of a Staphylococcus species resistant to fluoroquinolones. Because of nonresponse to medical management, the patient underwent therapeutic penetrating keratoplasty. Staphylococcal infection of the cornea may appear as a ring-like infiltra...

To compare the prevalence of various staphylococcal species in ocular infections [OIs (n = 105)] and in normal healthy conjunctiva [NC (n = 51)]. Antibacterial susceptibility profile of the isolates and prevalence of mecA gene among the isolates were also compared. A prospective, comparative, experimental study. Antibiotic susceptibility was determ...

To determine whether liquid culture media are helpful in the diagnosis of infectious keratitis. Retrospective noncomparative case series. This is a retrospective review of microbiology records of 114 corneal scraping samples from infectious keratitis patients. Samples were processed by corneal smear microscopy (potassium hydroxide with calcofluor w...

To analyze the clinical presentation, microbiological evaluation, and management of post-cataract surgery sclerocorneal tunnel infection. This is a retrospective chart review of 11 patients with sclerocorneal tunnel infection after cataract surgery, managed between November 2006 and October 2009. The clinical characteristics and treatment outcomes...

Bevacizumab is widely used for several ocular conditions, including age-related macular degeneration, diabetic retinopathy, corneal neovascularization, neovascular glaucoma, and retinopathy of prematurity. We describe a 14-year-old patient with Stevens-Johnson syndrome in whom subconjunctival injection of bevacizumab in one eye caused bilateral reg...

To report the clinical and microbiological profile of patients with microsporidial keratoconjunctivitis in a tertiary eye care centre in India. A retrospective analysis of medical records of all cases of microbiologically confirmed microsporidial keratoconjunctivitis, who presented between March 2007 and October 2010, was done. In a single-centre,...

Background / Purpose: Microsporidia are currently considered extremely specialized type of fungi (1). They cause a variety of human infections including ocular infections such as keratoconjunctivitis and stromal keratitis (2). They have been associated with seasonal epidemics of keratoconjunctivitis in immunocompetent individuals similar to adeno...

Medical and microbiology records of seventeen patients (17 eyes), diagnosed as scleritis of infectious origin were reviewed; to study clinical features, predisposing risk factors, microbiologic profile and treatment outcome of infectious scleritis. The mean patient age was 52.3 ± 19.75 years. Twelve patients (70.6%) had history of trauma/prior surg...

Mycotic keratitis is an important cause of corneal blindness world over including India. Geographical location and climate are known to influence the profile of fungal diseases. While there are several reports on mycotic keratitis from southern India, comprehensive clinico-microbiological reports from eastern India are few. The reported prevalence...

This study aims to describe the clinical features and management of Nocardia scleritis. The authors retrospectively reviewed medical charts of three patients with microbiologically proven Nocardia scleritis and reviewed literature. All the patients presented with areas of well-demarcated, circumscribed abscess. No specific clinical feature could be...

We describe the adaptation of two-incision push-pull technique for performing continuous capsulorhexis in children's eyes with anterior capsules having large fibrous plaque in the center. This technique uses the fibrous plaque as a template for directing the force vectors to achieve a controlled tear and a continuous capsulorhexis. We used the tech...

Epidemic keratoconjunctivitis (EKC) occurs worldwide, affects a large number of individuals, is infectious, often seasonal and is usually known to be caused by human adenoviruses (HAdV serotype 4, 8, 19 and 37).1 Regular occurrence of EKC during the rainy seasons in Orissa, a state in eastern India, has been commonly observed by ophthalmologists in...

In this study, 60 fungal isolates from 60 patients with fungal keratitis were tested in vitro for their susceptibility to natamycin and the mean minimum inhibitory concentrations of natamycin (MICn) were correlated with clinical outcome. The mean MICn for various groups of fungi from patients with either early (<10 days) or late (≥ 10 days) present...

To correlate the culture results of corneal scrapings with culture and histopathology results of corneal tissues in therapeutic keratoplasty. A retrospective analysis of the culture results of corneal scrapings and corneal tissues of eyes that received therapeutic penetrating keratoplasty at a tertiary eye care center between December 2006 and Nove...

On examination, her best-corrected visual acuity was 20/70 OD. Slitlamp biomicroscopic examination of the right eye revealed conjunctival congestion, central stromal edema with Descemet folds, and keratic precipitates (Figure, A). Her intraocular pressure was normal. Based on results of the clinical evaluation, a diagnosis of herpetic viral endothe...

The differential diagnoses for phacolytic glaucoma are acute angle closure glaucoma, open angle glaucoma with uveitis, neovascular glaucoma, and glaucoma secondary to trauma. We report an unusual case where the dislocated cataractous lens firmly adherent to the corneal endothelium evoked a cellular reaction similar to phacolytic glaucoma but clinic...

To evaluate the efficacy of 0.02% polyhexamethylene biguanide (PHMB) in the treatment of keratoconjunctivitis caused by microsporidia. Prospective, double-masked, randomized, placebo-controlled clinical trial. One hundred forty-five patients in a single-center, institutional setting were recruited. Patients with superficial keratoconjunctivitis and...

To determine whether the inclusion of Sabouraud dextrose agar (SDA) is essential in the diagnosis of fungal keratitis. Corneal scrapings of 141 patients with microbial keratitis were smeared and cultured. Sheep blood agar (BA), chocolate agar (CA), SDA, non-nutrient agar (NNA) with Escherichia coli overlay, and brain heart infusion broth (BHI) were...

A 55-year-old man presented with profound swelling of the upper face and fever with a history of preceding insect bite. He was nonalcoholic and immunocompetent. Orbital CT showed a predominantly preseptal soft-tissue swelling. Empiric treatment with broad-spectrum antibiotics resulted in partial response. Surgical debridement and microbiologic eval...

To report a case of Acanthamoeba keratitis clinically presenting with a plaque-like lesion typical of fungal keratitis. A 55-year-old woman presented with a yellow plaque-like lesion on the cornea surrounded by an infiltrate. Smears and culture were taken. The plaque was removed and subjected to histopathological investigation. Smear, culture, and...