Sreenivasan Sasidharan

Sreenivasan Sasidharan
Universiti Sains Malaysia | USM · Institute for Research in Molecular Medicine (INFORMM)

BSc (Hons), MSc, PhD

About

261
Publications
129,167
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6,196
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Introduction
Medicinal plants Antioxidant activity Antimicrobial activity Toxicity studies MiRNA Animal model Nanoparticles
Additional affiliations
January 2007 - present
Universiti Sains Malaysia

Publications

Publications (261)
Article
The aim of this study was to investigate the different immunological and antimicrobial properties of breast milk from women with (W) or without (WO) vaginal yeast infections during pregnancy in 85 lactating women (W, n = 43; WO, n = 42). Concentrations of IL-10, IgA, IgM, IgG, EGF, and TGF-α were similar in both groups. However, breast milk of wome...
Article
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This study evaluates the volatile metabolic constituents and anticancer potential of essential oils distilled from the rhizomes of four Malaysian Zingiber officinale Roscoe (Zingiberaceae family) varieties (Bentong (BE), Cameron Highlands (CH), Sabah (SA), and Bara (BA)). The ginger essential oils were analyzed by gas chromatography coupled with qu...
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The prevalence of aged people has increased rapidly in recent years and brings profound demographic changes worldwide. The multi-level progression of aging occurs at diverse stages of complexity, from cell to organ systems and eventually to the human as a whole. The cellular and molecular damages are usually regulated by the cells; repair or degrad...
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Purpose The development of probiotics has seen tremendous growth over the years, with health benefits ranging from gut health to respiratory. We thus aimed to investigate the effects of probiotic Bifidobacterium lactis Probio-M8 (2 × 10¹⁰ log CFU/day) against acute respiratory tract infections (RTI), use of antibiotics, hospitalization period and e...
Article
Efforts in precision medicine to combat aberrant epigenome have led to the development of epigenetic targeting drugs. We have previously reported the capability of the BZD9L1 epigenetic modulator to impede colorectal tumour growth in vitro and in vivo through sirtuin (SIRT) inhibition. Although most benzimidazole derivatives are commonly less toxic...
Article
Polyalthia longifolia is known for its anti-oxidative properties, which might contribute to the antiaging action. Hence, the current research was conducted to evaluate the antiaging activity of P. longifolia leaf methanolic extract (PLME) in a yeast model based on morphology using microscopic approaches. Saccharomyces cerevisiae BY611 strain yeast...
Conference Paper
Full-text available
Plants produce variety of bioactive compounds. High phytochemical concentrations in medicinal plants, is critical in cell rejuvenation and anti-aging effects. Based on the medicinal plant Polyalthia longifolia‘s prominent antioxidative properties, this could contribute towards their antiaging benefits. Hence, the present study was undertaken using...
Article
Ethnopharmacological relevance Polyalthia longifolia var. angustifolia Thw. (Annonaceae) is commonly used in traditional medicine as a tonic for rejuvenation and exhibiting good antioxidant activities. Aim of the study: To evaluate P. longifolia methanolic leaf extract (PLME) antiaging activity at 1 mg/mL in Saccharomyces cerevisiae BY611 yeast. Ma...
Chapter
Herbal plants were used for the prevention and treatment of human diseases for thousands of years. Besides, herbal plants also play an important role as a vital source for developing novel drugs due to their special treatment values against various ailments and novel modes of action. There are anxieties amongst patients about using synthetic drugs...
Conference Paper
Full-text available
Polyalthia longifolia var. angustifolia Thw. (Annonaceae) is a medicinal plant associated with abundant health benefits and commonly used in traditional medicine. Hence, the present research was to explore the antiaging effect of P. longifolia methanolic leaf extract (PLME) at 1 mg/mL concentration in Saccharomyces cerevisiae of the BY611 yeast str...
Article
A new pair of fluorene derivatives, 8,8′-(((9,9-dihexyl-9H-fluorene-2,7-diyl)bis(methylene))bis(oxy))diquinoline and 3,3′-(((9,9-dihexyl-9H-fluorene-2,7-diyl)bis-(methylene))bis(oxy))dipyridine were synthesized and characterized. The molecular structures of the target fluorene derivatives were well elucidated by nuclear magnetic resonance (NMR), in...
Article
A new water-soluble chemosensor (L) made up by chalcone-coumarin hybrid framework was synthesized and the photophysical properties of probe L towards heavy metal ions were observed by naked-eye along with UV-vis and NMR spectroscopic techniques. The newly obtained chemosensor L showed an apparent colour change from yellow to cyan due to the presenc...
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Background: Kratom or Mitragyna speciosa Korth has been widely used to relieve the severity of opioid withdrawal in natural settings. However, several studies have reported that kratom may by itself cause dependence following chronic consumption. Yet, there is currently no formal treatment for kratom dependence. Mitragynine, is the major psychoacti...
Article
Cancer is one of the most important reasons for death worldwide. Cancer illness is increasing at a world-wide scale, signifying that the current cancer treatments need to be revisited to improve their efficacy and the development of less adverse toxicity drugs. Therefore, there is an urgent need for the development of more effective and less toxic...
Article
Medicinal plant Calophyllum inophyllum L. (Calophyllaceae) is traditionally used as Polynesian cosmetics antiaging agents in French Polynesia. The present research was conducted to evaluate the in vitro antiaging property of C. inophyllum fruit extract (CIFE) in Saccharomyces Cerevisiae BY611 Strain. The antiaging effect of CIFE at 1 mg/mL concentr...
Article
Mother Nature endowed this planet with abundant valuable medicinal plant resources, commonly used in traditional medicinal practices. Millions of grants money also used to conduct various types of research on medicinal plants around the world to prove their efficacy to cure various diseases. However, the currently available research evidence on the...
Article
Ethnopharmacological relevance: Medicinal plants are crucial to healing numerous illnesses. Elaeis guineensis Jacq (family Arecaceae) is a medicinal plant traditionally used for the treatment of wounds. Aim of the study: However, there are no scientific reports documented on the wound healing activities of this plant against Staphylococcus aureu...
Chapter
The comprehensive search for therapeutic agents, predominantly the green phytochemicals derived from plants, has recently gained high momentum. Phytochemicals are plant’s (phyto) chemical referring to various types of compounds that occur naturally in plants. Green phytochemicals from Mother Nature especially from medicinal plants are a rich source...
Article
The current study was conducted to validate the target proteins of down-regulated miR-221-5p in HeLa cells treated with P. longifolia leaf extract. The validation was done by label-free quantitative proteomics approaches, Gene Ontology (GO) and protein–protein interaction analyses after the cells transfected with miRNA mimics or miRNA inhibitor. Th...
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Both aging and diet play an important role in influencing the gut ecosystem. Using premature senescent rats induced by d-galactose and fed with high-fat diet, this study aims to investigate the effects of different potential probiotic strains on the dynamic changes of fecal microbiome and metabolites. In this study, male Sprague–Dawley rats were fe...
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Background Opiate addiction is a major health problem in many countries. A crucial component of the medical treatment is the management of highly aversive opiate withdrawal signs, which may otherwise lead to resumption of drug taking. In a medication-assisted treatment (MAT), methadone and buprenorphine have been implemented as substitution drugs....
Article
MicroRNAs are endogenous small non-coding-RNAs that control gene expression and cancer development. Previous studies reported that Polyalthia longifolia treatment induced apoptotic cell death in HeLa cells by down-regulation of miR-221-5p. Hence, the current study was conducted to validate the down-regulated miR-221-5p in HeLa cells. Functional ana...
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This study aimed to elucidate the targets and mechanisms of anti-staphylococcal effects from bioactive metabolites produced by lactic acid bacteria. We aimed to better understand the safety and efficacy of these bioactive metabolites in in vivo systems, typically at topical sites. The cell-free supernatant and protein-rich fraction from Lactobacill...
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MircroRNAs (miRNAs) are short non-coding RNAs with a length of approximately 20-22 nucleotides, which interact with their target mRNAs at 3’-untranslated region by partial pairing. The miRNA- mRNA interaction leads to induction of mRNA degradation and eventually translational inhibition. Thus, miRNAs play an important role in virtually all cellular...
Conference Paper
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Cancer is the second important reason for death globally. In addition, numeral unwanted side effects sometimes happen during chemotherapy. Medicinal plants based research is becoming popular because of their good anticancer effects and less or no side effects. Hence, the current research was reported the multiple beneficial actions of medicinal pla...
Article
Background Polyalthia longifolia which originates from India is rich with various useful phytochemicals which are valuable for human health. Accordingly, the current study was conducted to evaluate the combinational antimicrobial activity of P. longifolia ethyl acetate fraction (PLEAF) with ampicillin, antioxidant and cytotoxicity activities. Meth...
Conference Paper
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Medicinal plants always get greatest attention due to their good pharmacological activities such as anticancer activity. A number of herbal preparations either in wholesome or in their components have been shown to exhibit good anticancer activity. Hence, the current study was reported the anticancer activity of Polyalthia longifolia. The MTT assay...
Chapter
The extensive search for therapeutic agents derived from higher plants has recently accelerated owing to the antibiotic resistance crisis, which is a serious warning to human healthcare worldwide. It is estimated that antibiotic-resistant infections will lead to nearly ten million yearly deaths at the global level by the year 2050. Thus, it is wise...
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There has been a misconception on herbal consumption where many of these herbal drugs are assumed as non-toxic by the consumers. On that context, safety and toxicological approach should be relevantly considered and taken seriously since ignorance may lead to dangerous yet lethal consequences. Even though C. guianensis flower extracts has been empl...
Article
Several microscopy methods have been developed to assess the morphological changes in cells in the investigations of the mode of cell death in response to a stimulus. Our recent finding on the treatment of the IC 50 concentration (26.67 μ g/mL) of Polyalthia longifolia leaf extract indicated the induction of apoptotic cell death via the regulation...
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Leukemia is a type of blood cancer where abnormal and immature leucocytes are produced in the bone marrow. Methadone hydrochloride is a man-made drug that is commonly used in the maintenance treatment for drug addiction. The objective of this research was to determine the cytotoxic activity and apoptotic effects of methadone hydrochloride treatment...
Article
Background: Polyalthia longifolia is a popular medicinal plant and have been widely used as traditional remedy for centuries in curing of various ailments. The purpose of this study was conducted to determine the in situ antimicrobial synergistic effects between Polyalthia longifolia leaf ethyl acetate fraction (PLEAF) and ampicillin against MRSA...
Article
Recent scientific investigations on P. longifolia leaf extract revealed its anti-cancer property via the regulation of miR-221-5p. However, there were no further investigations performed to report the role miR-221-5p through the regulation of its target genes and the subsequent protein-protein interaction networking which is important in cancer pat...
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Green synthesis is a forthcoming trend in the nanotechnology field where classical methods of synthesis are now replaced by inexpensive and eco-friendly methods. In this study, a green method has been developed for the synthesis of silver nanoparticles (AgNPs) where AgNPs were synthesised using water-based facile hydrothermal method. Silver nitrate...
Conference Paper
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Abstract From ancient time traditional medicinal plants based herbal formula has made countless contribution to the well-being of the human population. Currently, more and more evidence showing the efficacy and potential of medicinal plants through the multidisciplinary modern approaches for the discovery of natural products anticancer agent. The...
Article
Reactive oxygen species (ROS) are common by-products of many natural oxidative biochemical processes in living cells. They are generated by breaking of a covalent bond of molecules. Conversely, environmental, lifestyle and pathological conditions also cause oxidative stress, a condition when free radicals production overwhelm antioxidant capability...
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Circular RNAs characterize a class of widespread and diverse endogenous RNAs which are non-coding RNAs that are made by back-splicing events and have covalently closed loops with no polyadenylated tails. Various indications specify that circular RNAs (circRNAs) are plentiful in the human transcriptome. However, their participation in biological pro...
Conference Paper
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The search for therapeutic agents and bio active compound to fight against emerging and existing diseases such as cancer is ongoing process. Various advanced microscopes are extensively employed in scientific methods to measure the cytological changes as occur in cell cytotoxicity. The microscopic images provides the topographical, morphological an...
Article
A new fluorene derivative, 1,1’-(9,9-dihexyl-9H-fluorene-2,7-diyl)bis (N,N-bis(pyridine-2-ylmethyl)methanamine has been successfully synthesized through condensation reaction of bis(2-pyridylmethyl)amine and 2,7-bis(bromomethyl)-9,9-dihexyl-9H-fluorene with exceptionally good yield (60%). The molecular structure of the synthesized compound was well...
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Background: Couroupita guianensis Aubl. has many therapeutic uses in the practice of traditional medicine. Objective: The current research was conducted to evaluate the anti-Candida albicans activity, antioxidant properties, and phytochemical constituents with the identification of antifungal active fraction from C. guianensis flower. Methods: Anti...
Chapter
Natural products such as natural food are the richest bio-resource of bioorganic compounds for modern medicines, nutraceuticals, food supplements and pharmaceutical applications. The research and application on natural food started with the extraction techniques that play an important role to the extraction quantity (Yield), quality (extracted phyt...
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Hypercholesterolemia is one of the primary risk factors for cardiovascular diseases. The use of lactobacilli probiotics to reduce blood cholesterol levels have been extensively reported. However, more information is needed to evaluate the possible mechanisms involved and to identify possible targets for further therapeutic development. In this stud...
Article
Phaleriama crocarpa (Scheff.) Boerl known as God's crown or Mahkota Dewa is a dense evergreen tree, indigenous to Indonesia and Malaysia. This plant has been frequently used in traditional system of medicine for the treatment of flu, rheumatism, heart diseases and cancer. Various parts of this plant such as the leaves, fruits and seeds were used fo...
Article
Apoptosis is a process of a programmed cell death in all mammalian cells. Gaining understanding into the modern methods for detection of apoptotic cells will let the development of therapeutic agents with the properties to induced apoptotic cells death in cancer therapy. In this context, modern methods for detection of apoptotic cells play an impor...
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Aloe vera gel was extracted, formulated and deposited as thin films by a facile solution process for memory application. The memory cell features a simple structure, in which a dried Aloe vera film was sandwiched in between an Ag top and ITO bottom electrode that was deposited on a glass substrate. Digital information can be encoded and stored as d...
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Aim To study the in vitro and in vivo anticandidal activity of nanocapsulated bovine lactoferrin. Materials & methods In vitro and in vivo antimicrobial activities were conducted to study the anticandidal activities of nanocapsules (NCs). Results The NCs showed good anticandidal activities. The disruption of cell wall and cell membrane was noted...
Article
Ethnopharmacological relevance: Phaleria macrocarpa (Scheff) Boerl, is a famous traditional medicinal plant which exhibited cytotoxicity against various cancerous cells. Traditionally, P. macrocarpa has been used to control cancer, impotency, hemorrhoids, diabetes mellitus, allergies, liver and heart disease, kidney disorders, blood diseases, acne...
Article
This study was conducted to investigate the anticancer effects and mechanism of Calophyllum inophyllum fruit extract against MCF-7 cells. C. inophyllum fruit extract was found to have markedly cytotoxic effect against MCF-7 cells in a dose-dependent manner with the IC50 for 24 h of 23.59 µg/mL. Flow cytometry analysis revealed that C. inophyllum fr...
Article
Lactoferrin has been known to have antimicrobial properties. This research was conducted to investigate the toxicity of Alginate/EUDRAGIT(®) S 100-enclosed chitosan-calcium phosphate-loaded Fe-bLf nanocapsules (NCs) by in vitro and in vivo assays. Brine shrimp lethality assay showed that the LC50 value of NCs was more than 1mg/mL which indicated th...
Conference Paper
Data specifies that varios medicinal plant have anti-cancer effect, but detailed mechanisms are not well understood. Recent studies have revealed anticancer potential of medicinal plant Polyalthia longifolia in HeLa cells. Hence, the current study was conducted to evaluate the standardized Polyalthia longifolia methanolic leaf extract (PLME) in rel...
Conference Paper
The increasing awareness towards sustainable development of electronics has driven the search for natural bio-organic materials in place of conventional electronic materials. The concept of using natural bio-organic materials in electronics provides not only an effective solution to address global electronic waste crisis, but also a compelling temp...
Article
Opioids are opium-like drugs which are commonly used as analgesics to treat moderate to severe pain. Apoptosis is a type of programmed cell death to remove unnecessary or damaged cells in an organism. Recently, the ability of opioids to induce apoptosis especially in cancer cell lines has gained the interest of many researchers. This fascinating fi...
Chapter
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Green bioactive polyphenol from Mother Nature especially from medicinal plants are a rich source of novel therapeutics. Therefore, the search for bioactive molecules from nature continues to play an important role in the invention of new medicinal agents. Most plants do provide an array of phytochemicals that may contribute to reduction of disease...
Article
Over the years a number of microscopy methods have been developed to assess the changes in cells. Some non-invasive techniques such as holographic digital microscopy (HDM), which although does not destroy the cells, but helps to monitor the events that leads to initiation of apoptotic cell death. In this study, the apoptogenic property and the cyto...
Conference Paper
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Abstract : There is currently an enormous surge of interest in the use of marine algae for biomedical application. The current research was conducted to evaluate the biomedical application of marine algae Gracilaria changii. Screening of the G. changii extract for antimicrobial activity showed that the extracts possesses significant antimicrobial...
Article
Ethnopharmacological relevance: Phaleria macrocarpa (Scheff) Boerl, is a well-known folk medicinal plant in Indonesia. Traditionally, P. macrocarpa has been used to control cancer, impotency, hemorrhoids, diabetes mellitus, allergies, liver and hearth disease, kidney disorders, blood diseases, acne, stroke, migraine, and various skin diseases. Ai...
Article
Deregulated angiogenesis plays a central role in the development and metastasis of solid cancers. Tumor vasculature expressing a variety of biomarkers offers some novel therapeutic options which can be selectively targeted with anti-angiogenic agents without significantly affecting the normal vasculature. However, anti-angiogenic agents currently a...
Article
Medicinal plants have been accepted as a gold mine, with respect to the diversity of their phytochemicals. Many medicinal plants extracts are potential anticancer agents. Polyalthia longifolia var. angustifolia Thw. (Annonaceae) is one of the most significant native medicinal plants and is found throughout Malaysia. Hence, the present study was int...
Article
Medicinal plants are sources of antioxidant compounds that have been reported to protect the human body from the adverse effects of free radicals. Therefore, the present study was intended to identify the DPPH (2, 2-diphenyl-1-picrylhydrazyl) free-radical scavenging constituent from the methanol extract of Polyalthia longifolia leaf using bioassay-...
Article
Phaleria macrocarpa (Boerl.) is a well-known medicinal plant and have been extensively used as traditional medicine for ages in treatment of various diseases. The purpose of this study was to determine the in situ cytotoxicity effect P. macrocarpa fruit ethyl acetate fraction (PMEAF) by using various conventional and modern microscopy techniques. T...
Conference Paper
Full-text available
Natural remedies such as algae have been used for the treatment of many diseases since ancient time all over the world. The current research was conducted to evaluate the in vitro and in vivo antimicrobial activity of marine algae Gracilaria changii. Screening of the G. changii extract for antimicrobial activity showed that the extracts possess sig...
Article
Full-text available
The therapeutic potential of Cassia surattensis in reducing free radical-induced oxidative stress and inflammation particularly in hepatic diseases was evaluated in this study. The polyphenol rich C. surattensis seed extract showed good in vitro antioxidant. C. surattensis seed extract contained total phenolic content of 100.99 mg GAE/g dry weight...
Article
Resistive switching behaviors in Aloe vera films are being explored for nonvolatile memory applications. A simple structure in which the Aloe vera films sandwiched in between a top and bottom electrode are used. The switching behaviors of the devices in which the Aloe vera film is dried at different temperatures and the roles of top electrode mater...
Article
Cassia (C.) surattensis Burm. f. (Leguminosae) is a flowering plant that has been traditionally used in many countries for food and medicinal purposes. The present study was conducted to determine in vitro cytotoxicity and anti-genotoxic activity of the seed extract of C. surattensis. The cytotoxic and anti-genotoxic activities were evaluated again...
Conference Paper
Full-text available
Isolation of green bioactive compounds from medicinal plants is one of the major emerging areas of scientific research in tropics. Medicinal plants are a promising source of novel green bioactive compounds as lead structures for bioactivity. The current analytical techniques and future perspectives (Sasidharan et al., 2010) of analytical methodolog...