Soumya Kanti Kar

Wageningen Livestock Research · Animal Nutrition

Replacement of current protein resources for novel or alternative sources may be one of the solutions to abolish the expected scarcity of dietary protein for animal feeds. However, little is known about the nutritional, protein composition, and potential functional value of such novel or alternative protein sources. In the present study, we used ad...

The genotype and external phenotype of organisms are linked by so-called internal phenotypes which are influenced by environmental conditions. In this study, we used five existing-omics datasets representing five different layers of internal phenotypes, which were simultaneously measured in dietarily perturbed mice. We performed 10 pair-wise correl...

Growth in world population will inevitably leads to increased demand for protein for humans and animals. Protein from insects and blood plasma are being considered as possible alternatives, but more research on their nutritional quality and health effects is needed. Here, we studied the effect of dietary protein source on metabolism and metabolic a...

Dietary protein sources can have profound effects on host-microbe interactions in the gut that are critically important for immune resilience. However more knowledge is needed to assess the impact of different protein sources on gut and animal health. Thirty-six wildtype male C57BL/6J mice of 35 d age (n = 6/group; mean ± SEM body weight 21.9 ± 0.2...

Organoids are in vitro model systems generated from tissues. Organoids express specific physiological functions associated with their original tissue location and they express tissue-segment-specific genes. The aim of this study was to culture pig organoids from different areas of intestinal segments: duodenum, ileum (with or without Peyers Patches...

The aim of this study was to use fecal metabolite profiling to evaluate the effects of contrasting sanitary conditions and the associated subclinical health status of pigs. We analyzed fecal metabolite profiles by nuclear magnetic resonance (1H NMR) from pigs aged 14 and 22 weeks. Pigs kept under low and high sanitary conditions differed in fecal m...

An experiment was performed to study the effects of a low inclusion level of Chlorella vulgaris (CV) biomass in broiler diets on performance, immune response related to inflammatory status, and the intestinal histomorphology. The study was performed with 120 Ross 308 male broiler chickens from 0–35 days of age. The broilers were housed in 12 floor...

The fatty acid content of microalgae, especially the high content of omega-3 fatty acids such as eicosapentaenoic acid (EPA, C20:5) and docosahexaenoic acid (DHA, C22:6), could enrich eggs when fed to laying hens. Moreover, the properties and bioactive components of omega-3 fatty acids could positively influence the health and production performanc...

Background Objective Our aim is to investigate the transcriptomic landscape of the intestinal organoids derived from pigs divergent for FE and their responses when challenged with Escherichia coli (E. coli). Conclusions Figure 1. Design of the experimental setup. Pigs were selected for a low or high feed efficient (FE) phenotype. Tissues were harve...

Black soldier fly (Hermetia illucens; BSF) larvae as dietary protein source have the ability to deliver nutrients and could possess functional properties that positively support animal productivity and health. More knowledge, however, is needed to assess the impact of feeding a BSF based diet on gut and animal health. Sixteen post-weaned male pigs...

Organoids are self-organizing, self-renewing three-dimensional cellular structures that resemble organs in structure and function. They can be derived from adult stem cells, embryonic stem cells, or induced pluripotent stem cells. They contain most of the relevant cell types with a topology and cell-to-cell interactions resembling that of the in vi...

Black soldier fly larva (BSF, Hermetia illucens L.) meal is considered as an alternative protein source with has the potential to partially replace conventional protein source such as soybean meal (SBM) in livestock species, including pigs. Measuring the profile of systemic amine metabolites by the ultraperformance liquid chromatography-tandem mass...

Feed efficiency (FE) is an important trait in livestock production. It is common to measure and improve FE via the feed conversion ratio (FCR), but the underlying biological mechanisms are still largely unknown and complex. Adult stem-cell derived organoids are powerful experimental models to study mammalian biology, and offer possibilities to adva...

Findings of this project are compiled in the presentation. This was presented to the funding agency.

This chapter discusses the concepts and the approaches taken with the quantification or mathematical modelling of rumen microbiota. The consequences from the perspective of mathematical modelling of microbial functionality at the whole rumen level are discussed as it is thought that models need to cover this level to predict fermentative and digest...

Here, we describe the use of monolayers of intestinal epithelial cells derived from intestinal organoids and transcriptomics to investigate the direct effects of dietary protein sources on epithelial function. Mechanically dissociated 3D organoids of mouse duodenum were used to generate a polarized epithelium containing all cell types found in the...

Major advances in analytical techniques and genomics have transformed our understanding of rumen microbiology. This understanding is of critical importance to livestock production since rumen function affects nutritional efficiency, emissions from ruminants (such as methane and nitrous oxide) as well as animal health. This collection reviews what w...

Background: The mammalian intestine is a complex biological system that exhibits functional plasticity in its response to diverse stimuli to maintain homeostasis. To improve our understanding of this plasticity, we performed a high-level data integration of 14 whole-genome transcriptomics datasets from samples of intestinal mouse mucosa. We used t...

Improving feed efficiency is an important trait in livestock production. So far, feed conversion ratio (FCR) has been used to improve feed efficiency. However, the underlying biological mechanisms of feed efficiency are still not clear and very complex. Adult stem-cell derived organoids have already proven to be powerful experimental models of mamm...

Protein sources may contain bioactive components with activities beyond their strictly nutritional properties. We have investigated the use of enteroids as a model to test the effects of different protein sources on the intestinal epithelium. Confluent 2D monolayers generated from mouse 3D enteroids from the duodenum were shown to form polarized mo...

Design of the experiment. (DOCX)

Feed intake (A) and bodyweight (B) of mice fed with different experimental diets. (DOCX)

Ingredient and calculated or analyzed nutrient composition of the experimental diets for mice, as fed basis. (DOCX)

Members of microbial family belonging to ‘others’ as shown in Fig 2. (DOCX)

The mean number of 16S rRNA sequence reads (A) and the number of OTU (B) counts detected in the ileal samples of mice fed with different experimental diets. (DOCX)

Concentrations of serum cytokines and chemokines in response to dietary treatment in mice. (DOCX)

Unique significantly expressed gene-sets (FDR <0.05) in ileum of mice fed with different protein sources compared to SBM-fed diet as shown in Fig 1. (DOCX)

Study on effect of zinc on the humoral immune response of piglets after classical swine fever virus vaccination was conducted on piglets and it was seen that significant increase of antibody level specific to classical swine fever (CSF) virus was observed in serum samples of Tamworth and desi crossbred piglets (10) after feeding of diet containing...

The present study was conducted to evaluate the fatty acid profile of subcutaneous adipose tissue of indigenous (Ghungroo and Assam local) and crossbred (HampshireXGhungroo; HampshireXNiang Megha, and HampshireXAssam local) pigs. Fat samples (six numbers per genetic group) were collected from organized slaughter houses and local markets in and arou...