Souhaila Bouatra

University of Alberta | UAlberta · CCIS

In the present review, we discuss the results of the research we have done over the last decade on multi-porphyrinic architectures bearing a nucleotidic backbone, compare all the data, offer a novel overview, and emphasize common capabilities of nucleosides appearing in various systems, providing a new insight on the pre-organizing capabilities of...

Flexible linkers such as uridine or 2′-deoxyuridine pre-organize bis-porphyrins in a face-to-face conformation, thus forming stable sandwich complexes with a bidentate base such as 1,4-diazabicyclo[2.2.2]octane (DABCO). Increased stability can be even greater when a dinucleotide linker is used. Such pre-organization increases the association consta...

Bis-porphyrins with flexible linkers such as uridine or 2'-deoxyuridin pre-organize in a face-to-face conformation and form stable sandwich complexes with bidentate base such as DABCO. The gain in stability can be even greater when a dinucleotide linker is used. Such pre-organization increases the association constant by one to two orders of magnit...

(15)N-labeled rosette nanotubes were synthesized and investigated using high-field solid-state NMR spectroscopy, X-ray diffraction, atomic force microscopy, and electron microscopy. The results established the H-bond network involved in the self-assembly of the nanostructure as well as bound water molecules in the nanotube's channel.

Saliva is a clear, watery biofluid produced by the salivary glands to protect and lubricate the oral cavity. While mostly composed of water (99 %), the chemical composition of saliva is known to change quite dramatically in response to a variety of different physiological states, stimuli, insults and stressors. Unfortunately, among the human bioflu...

We report herein the synthesis of a dinucleotide bearing pendant porphyrins dedicated to adopt a pre-organized coformation with face-to-face porphyrins, and capable to self-organize in a stable sandwich type complexe with bidentate base such as DABCO. Earlier studies demonstrated that a peptidic linker does not provide sufficient pre-organization t...

Background: Heart failure (HF) with preserved ejection fraction (HFpEF) is increasingly recognized as an important clinical entity. Preclinical studies have shown differences in the pathophysiology between HFpEF and HF with reduced ejection fraction (HFrEF). Therefore, we hypothesized that a systematic metabolomic analysis would reveal a novel meta...

The objective of this study was to determine commonalities and differences in the metabolome in non‐pregnant pigs and in early (EG), late (LG) and entire pregnancy during the study of amino acid (AA) requirements. Each pig was fed 6 levels of test AA at maintenance and in both EG and LG. The metabolome of 331 plasma samples from 19 sows in their 2n...

Urine has long been a "favored" biofluid among metabolomics researchers. It is sterile, easy-to-obtain in large volumes, largely free from interfering proteins or lipids and chemically complex. However, this chemical complexity has also made urine a particularly difficult substrate to fully understand. As a biological waste material, urine typicall...

NMR Compound Identification and Quantification. (DOC)

Characterization of Thiols from Urine. (DOC)

Direct Flow Injection/LC-MS/MS Compound Identification and Quantification. (DOC)

Trace Element Analysis Using ICP-MS. (DOC)

GC-MS Compound Identification and Quantification. (DOC)

Characterization of Isoflavones from Urine. (DOC)

Brassica carinata (Ethiopian mustard) has previously been identified as a potential crop species suitable for marginal land in the North American prairies due to its relatively high salt tolerance. Two genetically related B. carinata lines with brown-seeded (BS) and yellow-seeded (YS) phenotypes were assessed for their tolerance to sodium sulfate....

The Human Metabolome Database (HMDB) (www.hmdb.ca) is a resource dedicated to providing scientists with the most current and comprehensive coverage of the human metabolome. Since its first release in 2007, the HMDB has been used to facilitate research for nearly 1000 published studies in metabolomics, clinical biochemistry and systems biology. The...

Dairy cows fed high-grain diets during early lactation have a high incidence of metabolic disorders. However, the precise mechanism(s) of how grain feeding causes disease is not clear. In an effort to understand how this diet transition alters the rumen environment and potentially leads to certain metabolic disorders in dairy cattle, we undertook a...

Dairy cows fed high-grain diets during early lactation have a high incidence of metabolic disorders. However, the precise mechanism(s) of how grain feeding causes disease is not clear. In an effort to understand how this diet transition alters the rumen environment and potentially leads to certain metabolic disorders in dairy cattle, we undertook a...

Our previous work showed that infection of Nicotiana tabacum (tobacco) plants with tobacco mosaic virus (TMV) results in transgenerational changes in plant phenotype. To test whether the progeny of infected (PI) plants have changes in metabolites, we analyzed their metabolic profiles using NMR spectroscopy and gas chromatography–mass spectrometry....

The rumen is a unique organ that serves as the primary site for microbial fermentation of ingested plant material for domestic livestock such as cattle, sheep and goats. The chemical composition of ruminal fluid is thought to closely reflect the healthy/unhealthy interaction between rumen microflora and diet. Just as diet and feed quality is import...

Supplementary materials and methods information regarding sample preparation, analysis and combinatorial lipid reconstruction. (DOC)

Targeted Lipid Mediator Surrogate Recoveries. (DOC)

Concentrations of total fatty acids in healthy serum by Lipomics and compared with the results of Kuriki et al . [9] . (DOC)

Concentrations of phosphatidylcholines in healthy serum (µM) by DFI MS/MS (Biocrates kit). (DOC)

Comparison of the concentrations of phosphatidylcholines in healthy serum quantified by DFI MS/MS (Biocrates kit), by Quehenberger et al. [13] and estimated by CLR (most probable). (DOC)

Percentage distribution of plasma cholesteryl esters determined by Lipomics, by Kim et al. [10] , by Vercaemst et al. [11] and by Ohrvall et al. [12] . (DOC)

Mean and standard deviation (µM) seen over the 12-week sampling period for the 44 metabolites as measured for the 9 heart transplant patients. (DOC)

Continuing improvements in analytical technology along with an increased interest in performing comprehensive, quantitative metabolic profiling, is leading to increased interest pressures within the metabolomics community to develop centralized metabolite reference resources for certain clinically important biofluids, such as cerebrospinal fluid, u...

We report herein the binding studies of three bis-porphyrinic tweezers bearing nucleosidic linkers and a bis-porphyrinic dipeptide with two bidentate bases of different size, DABCO and 4,4' -bipyridine. In the nucleosidic series, the nucleoside confers, for two dimers out of three, sufficient preorganization to the dimers to enhance the association...

The Human Metabolome Database (HMDB, http://www.hmdb.ca) is a richly annotated resource that is designed to address the broad needs of biochemists, clinical chemists, physicians, medical geneticists, nutritionists and members of the metabolomics community. Since its first release in 2007, the HMDB has been used to facilitate the research for nearly...

Elaboration of bis-porphyrinic tweezers bearing nucleosidic linkers for molecular recognition

Les édifices multi-porphyriniques retiennent de plus en plus l’attention des chercheurs et leur compréhension n’a cessé de croître ces dernières années en raison de leur vaste domaine d’application, que ce soit pour l’élaboration de complexes collecteurs d’énergie lumineuse, pour la construction de fils moléculaires photoniques ou électroniques ain...

Detailed complexation studies of two rigid tweezers bearing mono- or tris-anthracenic spacers is reported, along with the synthesis and characterization of two new tweezers bearing flexible nucleosidic linkers. For flexible tweezers bearing the two porphyrins at O-5' and O-3' positions of the ribose, the association constant for the complexation of...

In addition to controlling the structure of multi-chromophoric arrays, monitoring the spatial orientation of the chromophores in artificial light harvesting devices is a challenge of growing interest. We report in this article our recent advances in this field. It is expected that a better understanding of the physicochemical properties of rigid co...