Sorina Claudia Popescu

Sorina Claudia Popescu
Mississippi State University | MSU · Department of Biochemistry, Molecular Biology, Entomology and Plant Pathology

PhD

About

119
Publications
8,515
Reads
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1,482
Citations
Citations since 2017
89 Research Items
785 Citations
2017201820192020202120222023050100150
2017201820192020202120222023050100150
2017201820192020202120222023050100150
2017201820192020202120222023050100150
Additional affiliations
August 2019 - present
Mississippi State University
Position
  • Professor (Associate)
August 2015 - present
Mississippi State University
Position
  • Professor (Assistant)
September 2009 - September 2015
Cornell University
Position
  • Adjunct Assistant Professor

Publications

Publications (119)
Article
Full-text available
Waterlogging is an important environmental stress limiting the productivity of crops worldwide. Cowpea (Vigna unguiculata L) is particularly sensitive to waterlogging stress during the reproductive stage, with a consequent decline in pod formation and yield. However, little is known about the critical processes underlying cowpea's responses to wate...
Article
Waterlogging causes various metabolic, physiological, and morphological changes in crops, resulting in yield loss of most legumes in rainfed and irrigated agriculture. However, research on cowpea genotypes using physiological and biochemical traits as a measure of tolerance to waterlogging stress is limited. We evaluated the impacts of 7 days of wa...
Article
Full-text available
Pseudokinases are thought to lack phosphotransfer activity due to altered canonical catalytic residues within their kinase domain. However, a subset of pseudokinases maintain activity through atypical phosphotransfer mechanisms. The Arabidopsis ILK1 is a pseudokinase from the Raf-like MAP3K family and is the only known plant pseudokinase with confi...
Preprint
Waterlogging is an important environmental stress limiting the productivity of crops worldwide. Cowpea ( Vigna unguiculata L) is particularly sensitive to waterlogging stress during the reproductive stage, with a consequent decline in pod formation and yield. However, little is known about the critical processes underlying cowpea’s responses to wat...
Article
Full-text available
Waterlogging induces growth and developmental changes in sensitive crops such as cucumber (Cucumis sativus L.) during early plant development. However, information on the physiological mechanisms underpinning the response of cucumber plants to waterlogging conditions is limited. Here, we investigated the effects of 10-day waterlogging stress on the...
Chapter
Proteins can be covalently modified by a broad range of highly reactive chemicals and redox mechanisms. Reversible redox-mediated post-translational modifications of sensitive cysteine thiol groups in proteins impact protein characteristics such as interaction behavior and activity state. Evaluating the response of proteins to redox perturbation or...
Preprint
Targeted proteolysis activities activated during the plant immune response catalyze the synthesis of stable endogenous peptides. Little is known about their biogenesis and biological roles. Herein, we characterize an Arabidopsis thaliana mutant top1top2 in which targeted proteolysis of immune-active peptides is drastically impaired during effector-...
Preprint
While most pseudokinases lack phosphotransfer activity due to altered catalytic residues within their kinase domain, a subset of pseudokinases maintain kinase activity through atypical mechanisms. The Arabidopsis ILK1 is a pseudokinase from the Raf-like MAP3K family and is the only known plant pseudokinase with confirmed kinase activity. ILK1 activ...
Article
Full-text available
Invading pathogens interact with plant-associated microbial communities, which can be altered under the pressure of pathogen infection. Limited information exists on plant–microbe interactions occurring during natural outbreaks in agricultural fields. Taproot decline (TRD) of soybean is an emerging disease caused by Xylaria necrophora. TRD disease...
Preprint
Full-text available
Waterlogging is an important environmental stress limiting the productivity of crops worldwide. Cowpea ( Vigna unguiculata L) is particularly sensitive to waterlogging stress during the reproductive stage, with a consequent decline in pod formation and yield. However, little is known about the critical processes underlying cowpea’s responses to wat...
Preprint
Waterlogging induces growth and developmental changes in sensitive crops such as cucumber ( Cucumis sativus L.) during early plant development. However, information on the physiological mechanisms underpinning the response of cucumber plants to waterlogging conditions is limited. Here, we investigated the effects of 10-day waterlogging stress on th...
Preprint
Full-text available
Waterlogging induces growth and developmental changes in sensitive crops such as cucumber ( Cucumis sativus L.) during early plant development. However, information on the physiological mechanisms underpinning the response of cucumber plants to waterlogging conditions is limited. Here, we investigated the effects of 10-day waterlogging stress on th...
Article
Full-text available
Upon pathogen infection, receptors in plants will activate a localized immune response, the effector-triggered immunity (ETI), and a systemic immune response, the systemic acquired response (SAR). Infection also induces oscillations in the redox environment of plant cells, triggering response mechanisms involving sensitive cysteine residues that su...
Article
Protein homeostasis (proteostasis) is crucial for proper cellular function, including the production of peptides with biological functions through controlled proteolysis. Proteostasis has roles in maintenance of cellular functions and plant interactions with the environment under physiological conditions. Plant stress continues to reduce agricultur...
Chapter
The complexity in chemical composition alongside the genomic complexity of crop plants poses significant challenges for the characterization of their proteomes. This chapter provides specific methods that can be used for the extraction and identification of proteins from sweet potato, and a proteogenomic method for the subsequent peptide mapping on...
Article
Two complementary protein extraction methodologies coupled with an automated proteomic platform were employed to analyze tissue-specific proteomes and characterize biological and metabolic processes in sweetpotato. A total of 74,255 peptides corresponding to 4,321 nonredundant proteins were successfully identified. Data were compared to predicted p...
Article
Full-text available
Background Several methods to handle data generated from bottom-up proteomics via liquid chromatography-mass spectrometry, particularly for peptide-centric quantification dealing with post-translational modification (PTM) analysis like reversible cysteine oxidation are evaluated. The paper proposes a pipeline based on the R programming language to...
Article
Full-text available
Background Gene families are sets of structurally and evolutionarily related genes – in one or multiple species – that typically share a conserved biological function. As such, the identification and subsequent analyses of entire gene families are widely employed in the fields of evolutionary and functional genomics of both well established and new...
Article
Full-text available
Plant protein kinases form redundant signaling pathways to perceive microbial pathogens and activate immunity. Bacterial pathogens repress cellular immune responses by secreting effectors, some of which bind and inhibit multiple host kinases. To understand how broadly bacterial effectors may bind protein kinases and the function of these kinase int...
Data
Characterization of KEIs’ roles in plant innate immunity. A. Measurement of transcript accumulation following viral-induced gene silencing of KEI homologs in Nicotiana benthamiana by quantitative RT-PCR, in control (EC1)-silenced plants (black columns), and KEI-silenced plants (white columns). Asterisks denote significance (p < 0.01). B. Bacterial...
Data
A list of KEIs and interacting effector(s). The list was used to generate the K-E network from Fig 2A. K-E, kinase–effector; KEI, Kinase Effector Interactor. (DOCX)
Data
The table used to generate Fig 6E. (XLSX)
Data
The supplemental methods file contains details on (1) SLCA data analysis, (2) statistical testing of bacterial growth and PCD assays for VIGS-silenced KEIs, (3) correlation of immune responses for KEIs with significant stress phenotype, (4) co-occurrence pattern analysis for immune pathways inference, and (5) KEI signaling network analysis. KEI, Ki...
Data
Processing and visualization of protein interaction data from the high-throughput SLCAs. A. The luminescence signals of technical replicates located in the same 96-well plate show low variability. Measurements of correlation coefficients on data sets from biological replicates (T1 to T4) show high repeatability: T1–T2 (r = 0.98), T3–T4 (r = 0.962),...
Data
Characterization of KEIs roles in PCD. A. Histogram showing the intensity of cell death measured in control (EC1) or KEI-silenced Nicotiana benthamiana leaves following challenge with a bacterial strain triggering effector-induced immunity, Pseudomonas syringae D29E +HopQ1-1, or after overexpression of the MAP2Ks, MKK7 or MKK9. Cell death intensity...
Data
Topological parameters of signal-specific networks. A. Topological and statistical parameters of the signal-specific networks shown in Fig 5C. B. Visualization of average BC of the signal-specific networks shown in Fig 5C. All parameters were calculated using the NetworkAnalyzer in Cytoscape v. 3.6.1. BC, betweenness centrality. (TIF)
Data
A list of the focus KEIs including the number, https://solgenomics.net/ ID, the top BLAST hit in the Arabidopsis genome, known symbol, kinase structural class in PlantsP database (https://www.hsls.pitt.edu/obrc/index.php?page=URL1100616073), and their bacterial effector interactors. BLAST, Basic Local Alignment Search Tool; KEI, Kinase Effector Int...
Data
The table used to generate S2A Fig. (XLSX)
Data
The table used to generate S4B Fig. (XLSX)
Data
The table used to generate Fig 6A. (XLSX)
Data
Characteristics of pairwise K-E interactions measured in the SLCAs. A. A scatter chart showing the distribution of values for the interaction strength (fold change versus control) for all K-E interactions tested. B. The distribution of KEIs across protein kinase families. Percentages represent the number of kinases interacting with effectors from e...
Data
The table used to generate Fig 2C. (XLSX)
Data
The table used to generate Fig 2D and S2B Fig. (XLSX)
Data
The table used to generate S1 Fig. (XLSX)
Data
The table used to generate S4A Fig. (XLSX)
Data
The table used to generate S5B Fig. (XLSX)
Data
The table used to generate Fig 6D. (XLSX)
Data
Primary data for all phenotypic assays performed in this study: Bacterial growth assay, ETI and MKK overexpression cell death measurements, and PTI cell death suppression assays. ETI, effector-triggered immunity; MKK, MAP kinase kinase; PTI, PAMP-triggered immunity. (XLSX)
Data
MATLAB code for SLC data analysis. SLC, split-luciferase complementation. (PDF)
Data
Alignment and tree files for the phylogenetic tree in Fig 4F. (RAR)
Data
VIGS amplicons used in this study. The table lists the gene lab ID, the sequences of the PCR oligonucleotides used for amplification, the expected size of the PCR amplicon, the gene ID in tomato and Nicotiana benthamiana, the number of targeted genes, and the length of overlap between the target and PCR amplicon. The last two columns list potential...
Data
The table used to generate Fig 4E. (XLSX)
Data
The table used to generate Fig 6F. (XLSX)
Data
The table used to generate S3B Fig. (XLSX)
Data
The table used to generate S3C Fig. (XLSX)
Article
Full-text available
A surge in the accumulation of oxidants generates shifts in the cellular redox potential during early stages of plant infection with pathogens and activation of effector-triggered immunity (ETI). The redoxome, defined as the proteome-wide oxidative modifications of proteins caused by oxidants, has a well-known impact on stress responses in metazoan...
Poster
Sweetpotato (Ipomoea batatas, (L.) Lam) is a perennial dicotyledonous plant from the morning glory family (Convolvulaceae) cultivated as an annual crop. At present, only a sparse collection of genomic resources and annotations exists for sweetpotato; this includes a haplotype-resolved assembly of I. batatas genome, assembly and gene annotations of...
Data
List of identified metabolites from shoots and roots of wild-type and PCS1 knockout mutant (cad1-3) grown under control conditions or after Cd exposure for 24 h. The analysis was performed in negative ion mode (LC-ESI-MS). The list includes the fragmentation patterns (MS/MS and MS3) and the putative identifications with the corresponding references...
Data
List of identified auxins from shoots and roots of wild-type and PCS1 knockout mutant (cad1-3) grown under control conditions. The analysis was performed in negative ion mode, except for the indole-3-acetic acid methyl ester (MeIAA), recorded in positive ion mode. Selected reaction-monitoring conditions were for protonated or deprotonated auxin met...
Data
Multivariate analysis of metabolites from shoots and roots of wild-type (wt) and PCS1 knockout mutant (cad1-3) grown under control conditions or after Cd exposure for 24 h. The LC-ESI-MS-detected m/z features were used as X variables. The O2PLS-DA S-loading plots p1 vs. pq(corr)1 showed the correlation of the metabolites (black squares, the q part...
Article
The enzyme phytochelatin synthase (PCS) has long been studied with regard to its role in metal(loid) detoxification in several organisms, i.e., plants, yeasts and nematodes. It is in fact widely recognized that PCS detoxifies a number of heavy metals by catalysing the formation of thiol-rich oligomers, namely phytochelatins, from glutathione and re...
Article
Full-text available
Kinases facilitate detection of extracellular signals and set in motion cellular responses for plant adaptation and survival. Some of the energy utilized for kinase signal processing is produced through the activity of ion transporters. Additionally, the synergy between cellular ions and signal transduction influences plant response to pathogens, a...
Data
Model and information associated with 3D structure prediction of ankyrin repeat domains of ILK1 to ILK6.
Data
Information associated with superposition of the ankyrin repeat domains of ILK1 to ILK6.
Data
Model and information associated with 3D structure prediction of full length ILK1.
Data
Alignment of all RAF sequences from Arabidopsis thaliana using Clustal Omega. The three conserved kinase motifs that are changed in ILKs – the Gly loop, the HRDLxxxN motif in the subdomain VIb, and the DFG motif in the start of the activation domain (A-loop) – are highlighted.
Data
Interaction partners of the Arabidopsis ILKs.