Sophie Prosolek

Sophie Prosolek
University of Cambridge | Cam · School of Clinical Medicine

Doctor of Philosophy

About

10
Publications
8,062
Reads
How we measure 'reads'
A 'read' is counted each time someone views a publication summary (such as the title, abstract, and list of authors), clicks on a figure, or views or downloads the full-text. Learn more
93
Citations
Additional affiliations
October 2015 - October 2019
Institute of Food Research
Position
  • PhD Student

Publications

Publications (10)
Article
Background: The REal-time Assessment of Community Transmission-1 (REACT-1) study has provided unbiased estimates of swab-positivity in England approximately monthly since May 2020 using RT-PCR testing of self-administered throat and nose swabs. However, estimating infection incidence requires an understanding of the persistence of RT-PCR swab-posit...
Article
Full-text available
The SARS-CoV-2 pandemic continues to expand globally, with case numbers rising in many areas of the world, including the Indian sub-continent. Pakistan has one of the world’s largest populations, of over 200 million people and is experiencing a severe third wave of infections caused by SARS-CoV-2 that began in March 2021. In Pakistan, during the th...
Article
p class="MsoNormal">SARS-CoV-2 infections were rising during early summer 2021 in many countries associated with the Delta variant. We assessed RT-PCR swab-positivity in the REal-time Assessment of Community Transmission-1 (REACT-1) study in England. We observed sustained exponential growth with average doubling time (June July 2021) of 25 days dri...
Preprint
Full-text available
Background The prevalence of SARS-CoV-2 infection continues to drive rates of illness and hospitalisations despite high levels of vaccination, with the proportion of cases caused by the Delta lineage increasing in many populations. As vaccination programs roll out globally and social distancing is relaxed, future SARS-CoV-2 trends are uncertain. Me...
Preprint
Full-text available
Background Community surveys of SARS-CoV-2 RT-PCR swab-positivity provide prevalence estimates largely unaffected by biases from who presents for routine case testing. The REal-time Assessment of Community Transmission-1(REACT-1) has estimated swab-positivity approximately monthly since May 2020 in England from RT-PCR testing of self-administered t...
Preprint
Full-text available
Background: The COVID-19 pandemic continues to expand globally, with case numbers rising in many areas of the world, including the Eastern Mediterranean Region. Lebanon experienced its largest wave of COVID-19 infections from January to April 2021. Limited genomic surveillance was undertaken, with just twenty six SARS-CoV-2 genomes available for th...
Preprint
Full-text available
Background England entered a third national lockdown from 6 January 2021 due to the COVID-19 pandemic. Despite a successful vaccine rollout during the first half of 2021, cases and hospitalisations have started to increase since the end of May as the SARS-CoV-2 Delta (B.1.617.2) variant increases in frequency. The final step of relaxation of COVID-...
Preprint
Full-text available
The SARS-CoV-2 pandemic continues to expand globally, with case numbers rising in many areas of the world, including the Indian sub-continent. Pakistan has one of the worlds largest populations, of over 200 million people and is experiencing a severe third wave of infections caused by SARS-CoV-2 beginning in March 2021. Currently very few SARS-CoV-...
Preprint
Full-text available
Genomic surveillance for SARS-CoV-2 lineages informs our understanding of possible future changes in transmissibility and vaccine efficacy. However, small changes in the frequency of one lineage over another are often difficult to interpret because surveillance samples are obtained from a variety of sources. Here, we describe lineage dynamics and p...

Questions

Questions (3)
Question
I'd like to quantify the thiol status of cells treated with an alkylating agent. Is Ellman's reagent suitable for use with whole cell lysates? Is there a standard protocol for this assay? Limited info exists available online and each source quotes a different concentration/preparation of Ellman's reagent
Question
I'm running a seahorse fatty acid oxidation assay in THLE-2 human hepatocytes. The basal OCR is very low (~30pmol/min) and declines steadily over the course of the assay. I've attempted this assay several times, adjusting the starvation parameters to no avail; both the rep and I are pretty confident this is not caused by low cell number (they're in a confluent monolayer) or gradual cell death. I'm also pretty sure that the respective concentrations of oligomycin and FCCP are ok, as I've titrated these successfully with no problem whatsoever. Its been suggested that I adjust the media supplements, but I don't want to detract from the point of the assay by encouraging my cells to use glucose/glutamine rather than fatty acids. Has anyone else encountered this problem or have any suggestions with regards to troubleshooting? 
Thanks! 
Question
I'm relatively new to cloning and have limited experience in working with bacterial culture. I've cloned my Geneart CRISPR nuclease vector in TOP10 E.coli and now have the task of generating enough plasmid for transfection into mamalian cells. I've tried pretty much everything in the troubleshooting guide to increase plamsid yield from my purification kit (Purelink HQ) to no avail, so I think I may have to look to my culture methods instead. I hear that Terrific Broth is far superior for plasmid yield than LB, however all kits specifically state the LB should be used. Can anyone give me an insight into why this might be and whether TB might be preferential for my purpose? 
Thanks!

Network

Cited By