Songkun Su

Songkun Su
Fujian Agriculture and Forestry University | FAFU · College of Bee Science

PhD.

About

91
Publications
28,761
Reads
How we measure 'reads'
A 'read' is counted each time someone views a publication summary (such as the title, abstract, and list of authors), clicks on a figure, or views or downloads the full-text. Learn more
1,240
Citations
Citations since 2017
44 Research Items
843 Citations
2017201820192020202120222023050100150
2017201820192020202120222023050100150
2017201820192020202120222023050100150
2017201820192020202120222023050100150
Additional affiliations
June 1993 - September 2012
Zhejiang University
Position
  • Professor (Full)

Publications

Publications (91)
Article
Full-text available
The honey bee (Apis mellifera) plays vital ecological roles in the pollination of crops and the maintenance of ecological balance, and adult honey bees may be exposed to exogenous chemicals including heavy metals during their foraging activities. Cadmium (Cd) is regarded as a nonessential toxic metal and is readily accumulated in plants; honey bees...
Article
The neurotransmitters receptor gene are multifunctional modulators of behaviors in social insects, with significant effects among species and several dominant roles within the same species. Multiple amines receptors affect diverse behavioral and physiological processes in the honeybee. Apis mellifera ligustica is excellent bee species due to its re...
Article
Full-text available
One of the brain's primary functions is remembering and learning the information related to food and odour. Since biogenic amines discovery in invertebrate and vertebrate, dopamine is considered a key modulator and neurotransmitter involved in honeybees' olfactory learning. Dopamine (DA) has a major part to play in rewarding prediction, learning, i...
Article
Full-text available
The biological bases of wanting have been characterized in mammals, but whether an equivalent wanting system exists in insects remains unknown. In this study, we focused on honey bees, which perform intensive foraging activities to satisfy colony needs, and sought to determine whether foragers leave the hive driven by specific expectations about re...
Article
Full-text available
Objectives: Biogenic amines modulate the honeybees' behavioral development, especially olfactory learning behavior. Diverse behavioral protocols have been developed to investigate the olfactory learning behavior of bees to process appetitive olfaction information. Apis mellifera ligustica is a well-known euso-cial insect to examine the olfactory le...
Article
Full-text available
Objectives Biogenic amines modulate the honeybees' behavioral development, especially olfactory learning behavior. Diverse behavioral protocols have been developed to investigate the olfactory learning behavior of bees to process appetitive olfaction information. Apis mellifera ligustica is a well-known eusocial insect to examine the olfactory lear...
Article
Full-text available
Insects change their stimulus-response through the perception of associating these stimuli with important survival events such as rewards, threats, and mates. Insects develop strong associations and relate them to their experiences through several behavioral procedures. Among the insects, Apis species, Apis mellifera ligustica are known for their o...
Article
Full-text available
The ethological study of dance behaviour has yielded some findings since Karl Von Frisch discovered and interpreted the ‘dance language’ in the honey bee. However, the function and role of long non-coding RNAs on dance behaviour are hardly known until now. In this study, the differential expression patterns of lncRNAs in the brains of waggling danc...
Article
Full-text available
CRISPR/Cas9, a predominant gene-editing tool, has been utilised to dissect the gene function in Apis mellifera. However, only the genomic region containing NGG PAM could be recognised and edited in A. mellifera, seriously hampering the application of CRISPR technology in honeybees. In this study, we carried out the bioinformatics analysis for genom...
Article
Full-text available
Vairimorpha (Nosema) ceranae is the most common eukaryotic gut pathogen in honey bees. Infection is typically chronic but may result in mortality. Gut microbiota is a factor that was recently noted for gut infectious disease development. Interestingly, studies identified positive, instead of negative, associations between core bacteria of honey bee...
Article
Full-text available
In honey bees, complementary sex determiner (csd) is the primary signal of sex determination. Its allelic composition is heterozygous in females, and hemizygous or homozygous in males. To explore the transcriptome differences after sex differentiation between males and females, with genetic differences excluded, csd in fertilized embryos was knocke...
Preprint
Full-text available
Background: Vairimorpha (Nosema) ceranae is the most common eukaryotic gut pathogen in honey bees, Apis mellifera. Infection is typically chronic but may result in mortality. Additional factors may be involved in mortality, including the honey bee gut microbiota. Previous studies of V. ceranae and gut microbiota identified positive associations bet...
Article
The hypoharyngeal glands (HGs) are the major organ for synthesizing and secreting royal jelly (RJ) in nurse honeybees. To eliminate the influence of age, we explored the molecular basis of RJ production by determining the morphology and protein content, and comprehensively comparing the transcriptome of HGs of age-matched nurses and foragers obtain...
Article
Full-text available
The fungus Ascosphaera apis, an obligate fungal pathogen of honey bee brood, causes chalkbrood disease in honey bee larvae worldwide. Biological characteristics of the fungal pathogen and the molecular interactions between A. apis and honey bees have been studied extensively. However, little is known about the effects of A. apis infection on antiox...
Article
Full-text available
Biogenic amines and neuropeptides are important neurotransmitters and modulators in the peripheral and central nervous systems of several invertebrate species. Biogenic amines play essential roles in modulating physiological and behavioral processes as neuromodulators, neurotransmitters, and neurohormones. Biogenic amines such as octopamine, dopami...
Article
Full-text available
Sacbrood virus (SBV) of honey bees is a picornavirus in the genus Iflavirus. Given its relatively small and simple genome structure, single positive-strand RNA with only one ORF, cloning the full genomic sequence is not difficult. However, adding nonsynonymous mutations to the bee iflavirus clone is difficult because of the lack of information abou...
Article
In order to interpret the molecular mechanisms that modulating the organism variations and selection signatures to drive adaptive evolutionary changes are indispensable goals in the new evolutionary ecological genetics. Here, we identified the gene locus associated to royal jelly production through whole-genome sequencing of the DNA from eight popu...
Article
Full-text available
Chalkbrood is a type of common fungal disease that affects colony health, productivity, and pollination of honey bees. In this study, resistant and susceptible larvae towards chalkbrood were initially determined by using SNP C2587245T. We compared the transcriptome of the resistant and susceptible individuals challenged with Ascosphaera apis and fo...
Article
Full-text available
Background: Skin injury is inevitable in daily life. In recent years, with the increasing morbidity of diseases such as diabetes and metabolic disorders, chronic wounds have become a considerable challenge in clinical practice. Royal jelly, reported to have multifarious biological and physiological properties, has been used as a remedy for a varie...
Article
Nosema ceranae has become the dominant Nosema pathogen in the honey bees, Apis mellifera. It primarily infects and reproduces in midguts and reproduced N. ceranae spores travel to hindguts and leave hosts with feces. Hindguts have structural support for bacterial symbionts and biofilms, which harbor the densest bacteria of the entire bee. Although...
Article
Abstract Prevalence of honeybee viral diseases has recently been causing major problems in the beekeeping industry, causing economic losses worldwide. Honeybees are sus‐ ceptible to a variety of diseases and various pathogens. Among these pathogens, prevalence viruses, along with other factors, are seriously threatening the health of bee species. I...
Article
Full-text available
Prevalence of honeybee viral diseases has recently been causing major problems in the beekeeping industry, causing economic losses worldwide. Honeybees are susceptible to a variety of diseases and various pathogens. Among these pathogens, prevalence viruses, along with other factors, are seriously threatening the health of bee species. In the prese...
Article
Full-text available
Nosema ceranae infection induces energetic stress, malnutrition, and precocious foraging in Apis mellifera. This study investigated effects of N. ceranae infection on the gene expression, protein content, and enzyme activity in the hypopharyngeal gland of nurse bees. The 285 differentially expressed genes were found between N. ceranae-infected and...
Article
Full-text available
The synergistic interactions between the ectoparasitic mite Varroa destructor and Deformed wing virus (DWV) lead to the reduction in lifespan of the European honey bee Apis mellifera and often have been implicated in colony losses worldwide. However, to date, the underlying processes and mechanisms that form the multipartite interaction between the...
Article
Full-text available
The genetic diversity of honeybee populations (240 individual) Apis mellifera from ten various locations of China and Pakistan was investigated by using COI (Cytochrome Oxidase Subunit I) gene. After screening of sequences, BioEdit was used for the alignment, and these aligned sequences were employed to MEGA-7 software, to find out the phylogenetic...
Article
Full-text available
The forkhead box (Fox) gene family, one of the most important families of transcription factors, participates in various biological processes. However, Fox genes in Hymenoptera are still poorly known. In this study, 14 Fox genes were identified in the genome of Apis cerana . In addition, 16 (Apis mellifera) , 13 (Apis dorsata) , 16 (Apis florea) ,...
Article
Full-text available
Honey bee is a social insect. Its colony is mainly coordinated by the chemical signals such as pheromones produced by queen or brood. Correspondingly, the worker bee developed numerous complicated olfactory sensilla in antennae for detection of these colony chemical signals and nectar/pollen signals in foraging. With the normal development of new e...
Article
Full-text available
The Asian honeybee Apis cerana is one of two bee species that have been commercially kept with immense economic value. Here we present the analysis of genomic sequence and transcriptomic exploration for A. cerana as well as the comparative genomic analysis of the Asian honeybee and the European honeybee A. mellifera. The genome and RNA-seq data yie...
Article
To improve our understanding of the disturbed metabolic pathways and cellular responses triggered by honeybee venom stimulation, we compared the changes in serum metabolites rats, either stimulated or not by honeybee venom by performing 1H NMR spectrometry-based metabonomics to identify potential biomarkers. In this study, 65 metabolites were struc...
Article
Full-text available
Nosema ceranae causes widespread infection in adult workers of European honey bees, Apis mellifera, and has often been linked to honey bee colony losses worldwide. Previous investigations of honey bee immune response to N. ceranae infection were largely based on laboratory experiment, however, little is known about the immune response of honey bees...
Article
Full-text available
China is the largest royal jelly producer and exporter in the world, and high royal jelly-yielding strains have been bred in the country for approximately three decades. However, information on the molecular mechanism underlying high royal jelly production is scarce. Here, a cDNA microarray was used to screen and identify differentially expressed g...
Article
Full-text available
Chlorpyrifos is a widely used organophosphorus insecticide. The acute oral 24 h median lethal dose (LD50) value of chlorpyrifos in Apis mellifera and in Apis cerana was estimated to assess differential acute chlorpyrifos toxicity in the two bee species. The LD50 values of chlorpyrifos in A. mellifera and in A. cerana are 103.4 ng/bee and 81.8 ng/be...
Article
Royal jelly (RJ) possesses biological effects on varied organisms. The objective of this study was to investigate the effect of RJ on tumour growth with transcriptomic profile. RJ intake for 42 days did inhibit tumour growth in 4T1-bearing mice, and gene expression profile of tumour was determined by high-throughput RNA sequencing system. Out of 28...
Article
Acute toxicities (LD50s) of imidacloprid and clothianidin to Apis mellifera and A. cerana were investigated. Changing patterns of immune-related gene expressions and the activities of four enzymes between the two bee species were compared and analyzed after exposure to sublethal doses of insecticides. Results indicated that A. cerana was more sensi...
Article
The Sacbrood virus (SBV) is widely distributed in European honey bees, Apis mellifera. AcSBV, a distinct SBV strain in Asian honey bees (A. cerana) causes larva death before pupation and often depopulates colonies, leading to collapse. It is the most severe disease in A. cerana beekeeping. AcSBV infects A. cerana in most natural habitats, yet occur...
Data
: Primer sequences of the detected genes.
Data
: Information of 369 differentially expressed genes screened by gene chip.
Data
: KEGG analysis of the differently expressed genes.
Data
: The details of probes in microarray construction.
Data
: Standardized data of high and low royal jelly bees.
Article
Full-text available
Importance: Given the critical role of honey bees in the pollination of agricultural crops, it is urgent to develop strategies to prevent the colony decline induced by the infection of parasites/pathogens. Targeting parasites and pathogens directly by RNAi has been proved to be useful for controlling infections in honey bees but little is known ab...
Article
Survey results of colony losses of honey bee (Apis mellifera) have been reported worldwide in recent years. However, there has previously been no large-scale survey reported for China, the largest beekeeping country. Here we present the result of a three-year survey from 2010 to 2013, using the standard COLOSS questionnaires. In total, we received...
Article
Full-text available
Chalkbrood is a disease affecting honey bees that seriously impairs brood growth and productivity of diseased colonies. Although honey bees can develop chalkbrood resistance naturally, the details underlying the mechanisms of resistance are not fully understood, and no easy method is currently available for selecting and breeding resistant bees. Fi...
Article
Full-text available
Honey bees are very important eusocial insects and are involved in the pollination of many plants. Queen bees and worker bees can develop from the same fertilized eggs and are thus genetically identical despite their substantial behavioral and physiological differences. The mechanism governing developmental differences between worker and queen bees...
Article
Full-text available
Caste determination in the honeybee has been demonstrated to be decided by the royalactin in the royal jelly. And the differentially expressed coding genes in caste determination of the worker and queen larvae have also been well studied. Our experiments identified that lncRNAs are also differentially expressed in the caste determination of the lar...
Article
Scientists from Zhejiang University and engineers from the Zhejiang Sanyong Technology Company have combined to produce an advanced machine has been introduced for royal jelly extraction with the potential to reduce labour intensity and produce a healthy and safe end product.
Article
More than one million kg of royal jelly are produced in China every year. A system has been developed at Zhejiang University to produce high yields of royal jelly from a new strain of honey bees. Their method involves eight steps: maintaining a high-yielding strain of honey bee, using specially developed equipment, prolonging the royal jelly produc...
Article
We demonstrated that honey bee viruses including Deformed wing virus (DWV), Black queen cell virus (BQCV) and Israeli acute paralysis virus (IAPV) could infect and replicate in the fungal pathogen Ascosphaera apis that causes honey bee chalkbrood disease, revealing a novel biological feature of honey bee viruses. The phylogenetic analysis show that...
Article
Full-text available
Taste plays a crucial role in the life of honey bees as their survival depends on the collection and intake of nectar and pollen, and other natural products. Here we studied the tarsal taste of honey bees through a series of behavioral and electrophysiological analyses. We characterized responsiveness to various sweet, salty and bitter tastants del...
Article
Full-text available
Social caste determination in the honey bee is assumed to be determined by the dietary status of the young larvae and translated into physiological and epigenetic changes through nutrient-sensing pathways. We have employed Illumina/Solexa sequencing to examine the small RNA content in the bee larval food, and show that worker jelly is enriched in m...
Conference Paper
Royal jelly (RJ) is a thick, milky material which is confirmed to be a secretion product of both hypopharyngeal and mandibular glands of nurse bees. Multiple bioactivities have been found in RJ. Proteins are the main bioactive compositions of RJ and called major royal jelly proteins (MRJPs). MRJP1 is a member of MRJPs having multiple biological fun...
Article
Full-text available
Honey bee health is mainly affected by Varroa destructor, viruses, Nosema spp., pesticide residues and poor nutrition. Interactions between these proposed factors may be responsible for the colony losses reported worldwide in recent years. In the present study, the effects of a honey bee virus, Israeli acute paralysis virus (IAPV), on the foraging...
Article
Full-text available
Juvenile hormone acid methyltransferase (JHAMT) is an enzyme involved in one of the final steps of juvenile hormone biosynthesis in insects. It transfers a methyl group from S-adenosyl-L-methionine (SAM) to the carboxyl group of either farnesoic acid (FA) or JH acid (JHA). Several genes coding for JHAMT have been cloned and characterized from insec...
Data
Full-text available
Tests on the PCR amplification efficiency of primer pairs. Serial 8× dilutions of cDNA samples were used as templates to generate relative standard curves. The regression equation, determination coefficient (r2) and PCR amplification efficiency (E) were calculated and presented. A) test on the primer pair for the target gene AmJHAMT; B) test on the...
Article
Juvenile hormone acid methyltransferase (JHAMT) is an enzyme involved in one of the final steps of juvenile hormone biosynthesis in insects. It transfers a methyl group from S-adenosyl-L-methionine (SAM) to the carboxyl group of either farnesoic acid (FA) or JH acid (JHA). Several genes coding for JHAMT have been cloned and characterized from insec...
Article
Full-text available
Given the differences in ecology and biology between Apis cerana cerana and Apis mellifera ligustica, we first used the Illumina–Solexa deep sequencing technology to describe the differences in the heads of A. cerana cerana and A. mellifera ligustica foragers at the gene expression level. We obtained over 3.6 million clean tags per sample and found...
Article
Objective: To explore the application value of three-dimensional ultrasonography (3D US) in diagnosis of fetal intrauterine hypoxia. Methods: Sixty normal singleton fetuses (normal control group) between 25 and 32 gestational weeks underwent transabdominal 3D US. Cerebral blood flow volume index (CVf), renal blood flow volume index (RVf) and the ra...
Article
MicroRNAs (miRNAs) are endogenous small non-coding RNAs regulating gene expression in animals and plants. To find some differentially expressed miRNAs that may be associated with age-dependent behavioural changes in honey bees (Apis mellifera), we applied next-generation high-throughput sequencing technology to detect small RNAs in nurses and forag...
Article
A large volume of honey bee (Apis mellifera) tag-seq was obtained to identify differential gene expression via Solexa/lllumina Digital Gene Expression tag profiling (DGE) based on next generation sequencing. In total, 4,286,250 (foragers) and 3,422,327 (nurses) clean tags were sequenced, 24,568 (foragers) and 13,134 (nurses) distinct clean tags cou...
Article
Royal jelly (RJ) is a thick, milky material produced by both the hypopharyngeal and the mandibular glands of nurse honeybees. The main proteins of RJ, named apalbumins or major royal jelly proteins (MRJPs), have multiple biological functions. Apalbumin1 is the most abundant glycoprotein of RJ. In this study, Bacmid- apalbumin1 was constructed for A...