Songeli Menezes Freire

• Professor (Associate) at Federal University of Bahia

Decreased radioactive activity to be injected in individuals for diagnostic exams performed on Positrons emission Tomography associated with Computed Tomography is becoming mandatory since the patient protection is one of the pillars for Radiation Protection. The technological advance opens the possibility to demonstrate this activity reduction as...

Multiple Myeloma (MM) is a cytogenetically heterogeneous clonal plasma cell proliferative disease whose diagnosis is supported by analyses on histological slides of bone marrow aspirate. In summary, experts use a labor-intensive methodology to compute the ratio between plasma cells and non-plasma cells. Therefore, the key aspect of the methodology...

The pathogenesis of acute myeloid leukemia (AML) involves mutations in genes such as FLT3 and NPM1 , which are also associated with the prognosis of the disease. The immune system influences disease progression, but the mechanisms underlying the interaction between the immune system and AML are not clear. In this study, the profiles of lymphocytes...

Multiple Myeloma (MM) is a hematological malignancy characterized by the clonal proliferation of plasma cells within the bone marrow. Diagnosing MM presents considerable challenges, involving the identification of plasma cells in cytology examinations on hematological slides. At present, this is still a time-consuming manual task and has high labor...

Background and Aims: Hepatitis Delta virus (HDV) genotype 3 is responsible for outbreaks of fulminant hepatitis in Northeastern South America. This study investigates if systemic inflammatory molecules are differentially expressed in patients with advanced fibrosis chronically infected with Hepatitis Delta virusgenotype 3(HDV-3). Methods: Sixty-one...

Introduction and Objectives: HDV-3 is responsible for outbreaks of fulminant hepatitis in northeastern South America. There are no studies investigating immune responses in relation to liver damage caused by HDV-3. This study aimed to investigate if systemic inflammatory molecules (SIM) are differentially expressed in patients with advanced fibrosi...

Introduction and Objectives: Hepatitis E is an infection caused by the E virus (HEV) with the fecal-oral transmission. The spectrum of the disease varies from acute form to chronic evolution. There are few studies in the rural region of Bahia State. This study aimed to study the seroprevalence of hepatitis E in a city in a rural region in Brazil. M...

Introduction: Hepatitis E virus infection can lead to severe liver disease and unregulated hepatitis in patients with a history of previous chronic liver disease of different etiologies. Objective: To determine the seroprevalence of hepatitis E in untreated carriers of hepatitis C virus, hepatitis B virus, autoimmune hepatitis and in patients with...

A osteoartrite (OA) é uma doença articular muito comum. No Brasil, sua prevalência é de 16,9%, constituindo a causa de 30 a 40% dos atendimentos nos ambulatórios de Reumatologia e 7,5% de todos os afastamentos de trabalho, afetando 60% das pessoas com 65 anos ou mais e 80% daquelas com 75 anos ou mais, interferindo nas atividades da vida diária das...

Corynebacterium pseudotuberculosis is a bacillus that causes caseous lymphadenitis in small ruminants, leading to great losses to rural producers; thus, an efficient diagnosis is necessary for using disease control measures. This study aimed to evaluate the antigenic potential of four C. pseudotuberculosis recombinant proteins (rSodC, rPknG, rNanH,...

Diversos fatores influenciam o resultado de um procedimento cirúrgico, dentre eles o controle adequado da inflamação. Um processo inflamatório inapropriado pode resultar em desfechos desfavoráveis, como a má cicatrização com deiscências de suturas, cicatrizes queloides e hipertróficas, aderências teciduais intra-abdominais, torácicas, ou entre os c...

In this study, we investigated the capacity of the recombinant proteins SpaC, NanH, SodC, and PLD of C. pseudotuberculosis to trigger protective humoral and cellular immune responses against experimentally induced C. pseudotuberculosis infection in sheep. The antigens were produced in a heterologous system and were purified by affinity chromatograp...

Background l-Glutamine (l-GLN) is the most abundant amino acid in the human body. In hypercatabolic conditions, supplementation with l-GLN improves immunological function. Among the immune cells, the lymphocytes are the largest consumers of l-GLN in a catabolic disease state. Here, we evaluated the efect of l-GLN treatment on the expression of T an...

Viral hepatitis are still considered as public health problems. Hepatitis caused by HAV and HEV, spread by enteric transmission are, acute infections associated with precarious basic sanitation, inappropriate hygiene and consumption of contaminated food. Hepatitis caused by HBV and HCV viruses transmitted by parenteral /sexual route are associated...

SARS-COV-2 is the virus responsible for COVID-19 being declared a pandemic in March 2020 by the World Health Organization due the rapid spread representing a global health crisis. The disease is characterized by a wide clinical spectrum ranging from asymptomatic forms until severe viral pneumonia, which can to evolve to severe acute respiratory syn...

Introduction Hepatitis A virus (HAV) is a hepatotrophic virus of fecal-oral transmission. Occupational exposure in the health area is not considered a risk of HAV contagion. Adults are more likely to develop fulminant hepatitis. In Brazil, those over 20 years of age have a high prevalence of anti-HAV antibodies (IgGHAV). Objective To study the pre...

O uso das células-tronco mesenquimais é um passo que a medicina regenerativa dá na direção da recuperação parcial ou total de algumas lesões, além da capacidade de trazer de volta funções perdidas com a idade. Com esse objetivo são utilizadas diversas técnicas com uma variedade de bioprodutos como o plasma rico em plaquetas (PRP), plasma rico em fi...

TLL is a malignant and aggressive leukemia whose survival time is limited. It is known that 5% of individuals infected with HTLV-1, after many years of latency, may develop this disease. The mechanisms by which the evolution of clinical conditions occurs are unknown, and in Brazil there are few studies about ATLL, this some important themes will be...

ATLL is a malignant and aggressive leukemia whose survival time is limited. It is known that 5% of individuals infected with HTLV-1, after many years of latency, may develop this disease. The mechanisms by which the evolution of clinical conditions occurs are unknown, and in Brazil there are few studies about ATLL, this some important themes will b...

Introduction: Hepatitis A Virus (HAV) is a hepatotrophic virus with oral-fecal transmission. Occupational exposition to the virus is not common in the health practice and this way of contagious is not considered as a risk of infection. Adults have more probability to have fulminate clinical manifestations and in Brazil, the most of population older...

The potential use of bone marrow mesenchymal stromal cells (BM-MSCs) for the treatment of osteonecrosis in sickle cell disease (SCD) patients is increasing. However, convenient BM-MSC quantification and functional property assays are critical factors for cell-based therapies yet to be optimized. This study was designed to quantify the MSC populatio...

O uso do aminoácido L-glutamina (GLN), entre outros aminoácidos e biomoléculas, tem sido extensivo nas abordagens terapêuticas nos últimos anos. O objetivo desse estudo foi o de trazer à luz os principais achados da literatura nacional e internacional sobre o uso da glutamina, além de fundamentar a importância básica e clínica para a leitura crític...

Introduction: Toxoplasmosis is an asymptomatic disease that can lead to systemic disease in the fetus of pregnant women with primary infection. This study aimed to determine the prevalence of toxoplasmosis, associated factors, and correlation between the serology of pregnant women and their pets, in the municipality of Ilhéus, Bahia, Brazil. Meth...

strong>Introdução: A prevalência de hepatite B pode ser influenciada pelo comportamento sócio-cultural e a geração Baby Boomer (BB) (1945-1964) pode ter sido mais suscetível a esta infecção. Objetivos: Investigar a soroprevalência de marcadores para a infecção pelo VHB e resposta vacinal e associação com fatores de risco. Metodologia: Soro de indiv...

Corynebacterium pseudotuberculosis é uma bacilo Gram-positivo causador da linfadenite caseosa em caprinos e ovinos. Dentre os seus possíveis fatores de virulência estão as proteínas pld (Fosfolipase D), cpp (Serina-protease CP40), nanH (Neuraminidase H), sodC (Superóxido Dismutase C), spaC (Adesina SpaC) e pknG (Proteína cinase G). Objetivo: este e...

Caseous lymphadenitis (LC) is a chronic contagious disease caused by Corynebacterium pseudotuberculosis, which mainly affects goats and sheep. Vaccination is an effective but not yet well-established method, partly due to a lack of knowledge surrounding the most effective immunoprotective components. The present study aimed to quantify and compare...

Introduction: Latent tuberculosis infection diagnosis based on the release of interferon-gamma in cultures of peripheral blood cells stimulated with Mycobacterium tuberculosis antigens has replaced the tuberculin skin test in many countries with low tuberculosis prevalence. The IFN-γ production can be influenced by genetic polymorphisms, of which...

Objective: This study investigated the in vitro effects of the alkaloid dihydrochelerythrine (DHC), which is extracted from Zanthoxylum stelligerum, on the viability, proliferation, cell death and b-catenin, NFkB, STAT3/pSTAT3 and interleukins roles. Method: In vitro experimental models of human (U251 and GL-15) and murine (C6) glioblastoma cell...

Objective: To evaluate the impact of the use of the molecular test for Mycobacterium tuberculosis and its resistance to rifampin (Xpert MTB/RIF), under routine conditions, at a referral hospital in the Brazilian state of Bahia. Methods: This was a descriptive study using the database of the Mycobacteriology Laboratory of the Octávio Mangabeira S...

Education is crucial for the allocation of freedom of choices and autonomy by assigning the real dignity of individuals. The understanding of scientific and technological advances favors the reduction of harmful socio-political-economic-cultural inequalities among citizens in the population. In this proposal, there are thematic examples of bioethic...

A glutamina (GLN) é um aminoácido neutro, condicionalmente essencial e descrito como o mais abundante no plasma e tecido muscular humano, está envolvida em diversas funções metabólicas e vias de sinalização celular. É importante para a modulação da expressão de genes relacionados com a síntese e degradação de proteínas e a ativação de genes envolvi...

Introduction: Hepatitis C virus (HCV) and human T-lymphotropic virus type 1 (HTLV-1) coinfection occurs in many regions. However, few studies have focused on the natural history of HCV-induced liver disease in coinfected patients. Objective: To describe the clinical, epidemiological, and histopathological aspects of HTLV-1/HCV coinfection in Bra...

Introduction: Early and reliable laboratory diagnosis is very important to guide treatment of and control strategies for a disease. Some technical problems can occur due to laboratory facilities and methodological procedures. Many in vitro diagnoses are based on colorimetric methods such as the quantitative EnzymeLinked Immuno Sorbent Assay (ELISA)...

Stem cell therapy with bone marrow-derived mononuclear cells (BMMC) is an option for improving joint function in osteonecrosis of the femoral head (ONFH). Bone marrow-derived mesenchymal stromal cell (MSC) numbers and their osteogenic differentiation are decreased in patients with ONFH. However, whether this decrease also extends to the early stage...

The reference intervals for leukocytes and lymphocytes currently used by most clinical laboratories present limitations as they are primarily derived from individuals of North American and European origin. The objective this study was to determine reference values for peripheral blood B lymphocytes, T lymphocyte subsets (CD4+, CD8+, naïve, memory,...

!--[if gte mso 9]> Normal 0 21 false false false PT-BR X-NONE X-NONE MicrosoftInternetExplorer4 Introduction: <span style="font-size: 8.0pt; font-family: ";Calibri-Italic";,";sans-serif";; mso-bidi-font-family: Calibri-Italic;">It has been suggested that individuals exposed to Mycobacterium tuberculosis can eliminate the infection through the innat...

Neospora caninum causes cattle abortion and neurological symptoms in dogs. Although infection is usually asymptomatic, classical neurological symptoms of neosporosis may be associated with encephalitis. This parasite can grow in brain endothelial cells without markedly damages, but it can modulate the cellular environment to promote its survival in...

O objetivo deste trabalho foi realizar uma análise situacional sobre o Gerenciamento de Resíduos de Serviços de Saúde em unidades veterinárias na cidade de Salvador-Bahia. O estudo foi realizado no período de abril de 2007 a setembro de 2008. Um questionário foi elaborado e aplicado em 38 unidades veterinárias distribuídas pelo município de Salvado...

Normal 0 21 false false false MicrosoftInternetExplorer4 O objetivo deste trabalho foi realizar uma analise situacional sobre o Gerenciamento de Residuos de Servicos de Saude em unidades veterinarias na cidade de Salvador-Bahia. O estudo foi realizado no periodo de abril de 2007 a setembro de 2008. Um questionario foi elaborado e aplicado em 38 uni...

Objective: The aim of this study was to evaluate possible immunologic relationships between sickle cell anaemia (SCA) and periodontal inflammation and its impact on serum cytokines. Design: Twenty-five Brazilian children of African descent were involved in this study and divided in two groups: SCA (n=10): confirmed diagnosis of homozygous anaemi...

!--[if gte mso 9]> Normal 0 21 false false false PT-BR X-NONE X-NONE Introdução: Na estrutura da Organização Mundial de Saúde (OMS) existem 50 indicadores de saúde que avaliam os seus 192 países-membro. Entre os parâmetros de análise, as doenças de notificação compulsória caracterizam a situação de saúde de cada um desses países. Sabe-se que cerca...

VEGF and TGF-β1 are cytokines that stimulate tissue invasion and angiogenesis. These factors are considered as molecular targets for the therapy of glioblastoma. Bevacizumab, a recombinant humanized monoclonal antibody developed against VEGF, inhibits endothelial cell proliferation and vessel formation. Flavonoids obtained from Dimorphandra mollis...

A complete blood count is very useful in clinical diagnoses when reference ranges are well established for the population. Complete blood counts and allele frequencies of Ancestry Informative Markers (AIMs) were analyzed in Brazilians with the aim of characterizing the hematological values of an admixed population. Positive associations were observ...

span class="texto">A toxoplasmose é uma infecção causada pelo Toxoplasma gondii , que pode contaminar vários tipos celulares. A toxoplasmose congênita é considerada importante causa de mortalidade e morbidade infantis. No presente trabalho, foi avaliada a presença de anticorpos das classes IgM e IgG no soro de pacientes grávidas, correlacionando-se...

Porphyromonas gingivalis tem sido fortemente associado à gravidade das doenças periodontais e estimula respostas celulares e humorais no hospedeiro. Objetivo: Correlacionar os níveis de IgA, IgG e subclasses de IgG contra uma fração cromatográfica do extrato de Porphyromonas gingivalis ATCC33277 extract e descritores clínicos periodontais. Material...

12532 Background: Malignant gliomas are typically angiogenic and express greater amounts of angiogenic factors. Vascular endothelial growth factor (VEGF) and platelet derived growth factor (PDGF) are prominent glioblastoma-associated multifunctional cytokines that stimulate migration, invasion and angiogenesis. Their receptor inhibiton is considere...

Rutin is a flavonoid obtained from Dimorphandra mollis (Benth.), a medicinal Brazilian plant used as antioxidative, antihemorrhagic, and blood vessel protector. The present study has examined its effects on the viability and function of immune system cells in vitro. Rat spleen and thymus cells were cultured with 10 nM, 1 microM, and 10 microM of th...

The present study has examined the effects of arborinine, an alkaloid obtained from Erthela bahiensis, a Brazilian plant popularly used as diuretic, antidiabetic, antithermic and expectorant, on the viability and function of immune system cells in vitro using a murine model. Rat spleen and thymus cells were cultured with 10 nM, 1 microM, 10 microM...

Corynebacterium pseudotuberculosis is the causal agent of caseous lymphadenitis, a chronic illness that attacks goats and sheep characterized by pyogranulomas formation in lymph nodes and organs. Regarding the current knowledge of the pathogenesis of the caseous lymphadenitis, there is evidence that besides the humoral response the induction of a d...

Ascites formation is commonly associated with cancer, although it is also present in other diseases. The aim of this study was to evaluate the usefulness of vascular endothelial growth factor as a malignancy marker in ascites of different etiologies. The levels of VEGF in 32 malignant and in 31 non-malignant ascites were determined by enzyme immuno...

span class="texto">Diferentes técnicas para isolar e descrever antígenos das frações secretadas de superfície e somáticas de Corynebacterium pseudotuberculosis foram estudadas por SDS-PAGE e imunoblot, utilizando-se pool de soros de cabras naturalmente infectadas. Os antígenos secretados foram obtidos do sobrenadante de cultura da bactéria cultivad...

span class="texto">Um teste de ELISA indireto foi desenvolvido e padronizado para o diagnóstico de linfadenite caseosa em caprinos. Foi usado como antígeno o secretado de cultura de 48h de Corynebacterium pseudotuberculosis em caldo BHI. Para o estabelecimento do ponto de corte e cálculo da sensibilidade e da especificidade, foram usadas 31 amostra...

em>Corynebacterium pseudotuberculosis é um cocobacilo gram-positivo, patógeno intracelular facultativo de macrófagos, filogeneticamente relacionado com o Mycobacterium tuberculosis. É amplamente distribuído em algumas espécies de animais, causando a linfadenite caseosa em ovinos e caprinos. A linfadenite caseosa, de ocorrência mundial, é uma doença...

In the present study we determined the effect of the lectins concanavalin A (Con A), phytohemagglutinin (PHA) and pokeweed (PWM), frequently used in cell proliferation assays, on the proliferation of goat peripheral blood mononuclear cells (PBMCs). The use of a standardized Percoll gradient proved to be highly effective for the separation/enrichmen...

span class="texto">A utilização de sondas de DNA para análise genômica em fase sólida tem se expandido e ultrapassado as áreas da pesquisa, estendendo-se ao terreno do diagnóstico de rotina nas áreas de medicina humana e animal, bem como nas ciências forenses. Estas sondas têm sido utilizadas desde o surgimento da primeira técnica de hibridização d...

The catalytic properties of ornithine decarboxylase (ODC) from Leishmania mexicana as well as the interaction with its cofactor pyridoxal 5'-phosphate (PLP) and the irreversible inhibitor alpha-difluoromethylornithine (DFMO) have been studied using partially purified preparations of the enzyme obtained from parasite promastigotes. Leishmania extrac...