Solange I Mussatto

Solange I Mussatto
Technical University of Denmark | DTU · Department of Biotechnology and Biomedicine

Group Leader / Full Professor in Biobased Technologies and Sustainability

About

306
Publications
237,570
Reads
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14,501
Citations
Introduction
My research interests are focused on the development of new, efficient and sustainable process technologies for the production of fuels, chemicals, food ingredients, materials and enzymes. My research activities fall in the areas: 1) Biomass pretreatment and hydrolysis; 2) Fermentation with or without downstream process integration; 3) Adaptive laboratory evolution and physiology of microorganisms; 4) Biorefinery, and 5) Sustainability assessment. See more about my group at: www.bcbtgroup.com
Additional affiliations
January 2021 - February 2021
Technical University of Denmark
Position
  • Group Leader
Description
  • I am Head of the research group Biomass Conversion and Bioprocess Technology. My Research Group works on the development of sustainable processes for the production of valuable compounds for different industry sectors. Our activities include Biomass fractionation; Fermentation; Process Intensification; CO2 utilization; Downstream processing; Adaptive evolution; Microbial co-cultures; Biorefinery; Techno-economic and Life cycle analyses.
November 2016 - December 2020
Technical University of Denmark
Position
  • Group Leader
Description
  • My Research Group works on the development of sustainable processes for the production of valuable compounds. Our activities include Biomass fractionation; Fermentation; Downstream processing; Adaptive evolution; Biorefinery, and Techno-economic analysis.
July 2014 - October 2016
Delft University of Technology
Position
  • Research Assistant

Publications

Publications (306)
Book
Full-text available
Biomass Fractionation Technologies for a Lignocellulosic Feedstock-based Biorefinery reviews the extensive research and tremendous scientific and technological developments that have occurred in the area of biorefinering, including industrial processes and product development using 'green technologies', often referred as white biotechnology. As the...
Article
Full-text available
Development of affordable and low carbon biobased manufacturing depends critically on strategies that reduce cost and emission profiles. This paper indicates that efforts around the reduction of capital costs by intensification of process equipment need to be carefully weighed against the inherently fast increasing financial and climate costs of dr...
Article
Full-text available
Using lignocellulosic biomass hydrolysate for the production of microbial lipids and carotenoids is still a challenge due to the poor tolerance of oleaginous yeasts to the inhibitors generated during biomass pretreatment. In this study, a strategy of adaptive laboratory evolution in hydrolysate-based medium was developed to improve the tolerance of...
Article
Full-text available
This study investigated the fermentability of hemicellulosic hydrolysates obtained by intensified steam explosion pretreatment of switchgrass (Panicum virgatum) and Eucalyptus globulus. Xylitol and carotenoids were the high-value molecules produced by fermentation. The intensified pretreatment allowed to process a large amount of biomass per unit o...
Article
Innovation and technology are seen today as key points in the transition to a greener and more sustainable economy. In this sense, the development of new processes able to result in reduced emissions and levels of CO2 in the atmosphere has been considered essential to support this transition and, at the same time, promote economic growth. Several t...
Article
Biobased products are complete or partial derivatives from naturally occurring/growing materials such as agricultural, plants, and forest materials and serve as an alternative to traditional product derivatives of petroleum. Technically, biobased product counterparts can replace almost every fossil resource-based industrial material. Adopting life...
Article
Full-text available
This work evaluates the effect of feedstock type and composition on the conversion of lignin to liquid by solvolysis with formic acid as hydrogen donor (LtL), by analyzing the yields and molecular composition of the liquid products and interpreting them in terms of both the type and the preprocessing of the lignocellulosic biomass using chemometric...
Article
Production of cellulosic ethanol from lignocellulosic biomass leads to the generation of a hemicellulosic hydrolysate during the feedstock pretreatment. This hydrolysate is rich in sugars, but also contains inhibitory compounds (mainly acetic acid and phenolic compounds) in concentrations that may be toxic to microbial growth. Currently, this side-...
Article
This study evaluated the subcritical water hydrolysis (SWH) of brewer's spent grains (BSG) to obtain sugars and amino acids. The experimental conditions investigated the hydrolysis of BSG in a single flow-through reactor and in two sequential reactors operated in semi-continuous mode. The hydrolysis experiments were carried out for 120 min at 15 MP...
Article
This research aimed to study some properties of three flour alternative sources from rice flour (RF), banana flour (BF), and job’s tears flour (JTF) when applied for cookies preparation. Physicochemical properties including water absorption index (WAI), water solubility index (WSI), oil absorption capacity (OAC), and pasting profiles of the flours,...
Article
Full-text available
Anaerobic digestion (AD) can be a suitable alternative for agro-industrial by-products energy recovery, contributing to reducing environmental side effects caused by incorrect disposal of these materials. In this study, dry AD reactors were started-up with açaí seeds, brewer’s spent grains (BSG), and poultry feathers, all solid by-products generate...
Article
The use of lactic acid bacteria (LAB) and probiotic cultures in the breeding of animals such as poultry and swine are quite common. It is known that those strains can produce bacteriocins when grown in pure culture. However, the production of bacteriocin using co-culture of microorganisms has not been much studied so far. The present study contribu...
Article
Full-text available
Xylitol is a GRAS (Generally Recognized as Safe) polyol commonly used in the food industry and able to promote several benefits to the health. In addition, it can also be used as a building block molecule for the manufacture of different high-value chemicals. Currently, the commercial production of xylitol occurs by chemical route through the catal...
Article
Full-text available
The conventional method to produce process flavoring is non‐continuous, time consuming, and generates a high volume of effluent. This research aimed to evaluate the use of methionine, thiamine, and reducing sugars to develop process flavorings by direct extrusion, as a potential alternative to the conventional method. The mixed substrates consisted...
Article
Full-text available
This study evaluated the individual and combined effects of inhibitory compounds formed during pretreatment of lignocellulosic biomass on the growth of Bacillus subtilis. Ten inhibitory compounds commonly present in lignocellulosic hydrolysates were evaluated, which included sugar degradation products (furfural and 5-hydroxymethylfurfural), acetic...
Article
Full-text available
Fructooligosaccharides (FOS) can be used as feed prebiotics, but are limited by high production costs. In this study, low-cost sugarcane molasses was used to produce whole-cell biocatalysts containing transfructosylating enzymes by Aureobasidium pullulans FRR 5284, followed by FOS production from molasses using the whole-cells of A. pullulans . A....
Article
Breweries generate a high amount of brewer’s spent grains (BSG), which is a valuable feedstock for industrial applications. In the current study, a techno-economic assessment of the flow-through subcritical water hydrolysis reactor to produce sugars from BSG was performed. Simulations were done for a sequential hydrolysis process in three-extractor...
Article
This study assessed the subcritical water hydrolysis (SWH) of poultry feathers to recover amino acids. Experiments were conducted in a semi-continuous flow-through subcritical reactor (110 mL), which was operated over a range of temperatures (210 – 250 °C) and water flow rates (5 – 15 mL min–1), combined through a 2² central composite design, at co...
Article
Full-text available
The presence of toxic compounds in lignocellulosic hydrolysates is one of the main problems affecting the efficiency of hydrolysate-based fermentation processes. Understanding the effects of biomass-derived inhibitors on the performance of the microbial strain is essential to develop strategies able to result in an improved fermentation performance...
Article
The underlying study presents models for the optimization of operational conditions of lignocellulosic biomass pretreatment to facilitate the conceptual process design of biorefineries. Experiments for hydrothermal and dilute acid pretreatment are performed and analyzed. The highest xylose monomer yield obtained for dilute acid pretreatment is YXyl...
Article
This comprehensive review aims to elucidate the state of the art related to research trends regarding the use of brewer's spent grains (BSG), the main solid by-product generated in breweries. Its status, future opportunities, and emerging trends were evaluated by bibliometric analysis, which contributed to clarifying the knowledge and perspectives...
Article
This study investigated the production of process flavorings by direct extrusion method using a model system consisted of 80% (w/w) d-xylose and glycine, alanine, or valine at 20% (w/w). The extruder's barrel temperatures were controlled at 65, 80, and 50 °C. The aroma compounds in the obtained products were analyzed by gas chromatography-mass-spec...
Article
Full-text available
This study aimed to select fermentation conditions able to simultaneously maximize the production of lipids and carotenoids by oleaginous yeast cultivated in wheat straw hydrolysate. An evolved strain of Rhodosporidium toruloides with improved tolerance to toxic compounds present in hydrolysate medium was used. Experiments were performed in order t...
Article
Full-text available
This study aimed to evaluate the synthesis and application of heterogeneous catalysts based on heteropolyacids for 5-hydroxymethylfurfural (HMF) production from glucose. Initially, assays were carried out in order to establish the most favorable catalyst synthesis conditions. For such purpose, calcination temperature (300 or 500 • C), type of suppo...
Article
Transitioning from gasoline and petroleum-based products to biofuels and green chemicals is a paradigm shift that will lead to the development of the bioeconomy. In this sense, the role of biorefineries in countries like Brazil is very important as the country generates a huge amount of second generation (2G) biomass every year and also has an attr...
Article
This study presents a techno-economic assessment of bioenergy and fertilizer production by anaerobic digestion (AD) of brewer’s spent grains (BSG). Simulations were performed by integrating the production of biomethane, electricity, thermal energy, and fertilizer for five revenue conditions. BSG generation from different brewery industrial scales w...
Article
Currently, downstream processing of oleaginous yeast is expensive and unfeasible. To decrease processing costs and support the industrial application of this type of microorganism, this study proposes a new strategy for simultaneous recovery and further separation of lipids and carotenoids from oleaginous yeast. Assays were performed using the stra...
Article
Full-text available
This study investigated the effect of xylanase-assisted alkaline pretreatment (XAP) on the extraction of cellulose nanocrystal (CNCs) from energy cane (EC). Different concentrations of xylanase were tested to estimate the optimal enzyme loading required for pretreatment. XAP reduced the bleaching step from 7 to 5 h, generating a material with 93% w...
Chapter
Sustainable aviation fuels (SAF) are fuels that have potential to be sustainably produced and to generate lower carbon emissions when compared to the conventional kerosene. Wider SAF production and utilization represent nowadays an important step to move towards a more sustainable aviation industry, which has committed to significantly reduce their...
Article
Full-text available
Organic based cat litter becomes more and more popular in many countries including Thailand because of the concern on environmental and health impacts in cats and cat owners. Most of the cat litter sold in Thailand is manufactured and imported from abroad. Cassava trunk pith, the stele of cassava trunk, is the biomass obtained from agricultural was...
Article
Full-text available
L-asparaginase (ASNase) is a therapeutical enzyme used for treatment of acute lymphoblastic leukemia. ASNase products available in the market are produced by bacteria and usually present allergic response and important toxicity effects to the patients. Production of ASNase by yeasts could be an alternative to overcome these problems since yeasts ha...
Article
Full-text available
This study aimed to select the best reaction conditions to produce 5-hydroxymethylfurfural (HMF) from cellulose using heterogeneous catalyst based on a heteropolyacid (H 3 PW 12 O 40-HPW) and Nb 2 O 5. Initially, the influence of the temperature (160 or 200 • C), acetone:water ratio (50:50 or 75:25 v/v), cellulose load (5% or 10% w/v) and catalyst...
Article
This study evaluates the benefits of mineral fertilizers replacement for biodigested vinasse. Data from experimental anaerobic digestion (AD) of vinasse were applied to support the analysis. Based on previous experiments, this assessment assumed that vinasse production could reach 2.38 × 10⁷ m³/year generating around 66,585 MWh/year of electric ene...
Article
The agricultural and food industry sectors play essential roles in the global economy, but also generate significant amounts of wastes every year associated to their activities, which require an appropriate management. As an alternative, agro-industrial by-products can be used for obtaining valuable compounds such as amino acids, phenolic compounds...
Article
Full-text available
The article brings an analysis on published models used to calculate phase separation in aqueous two-phase systems (ATPS) based on Flory-Huggins (FH) theory, in terms of problem formulation and mathematical solving algorithm. An integrated algorithm is presented, showing different mathematical approaches of using the FH theory. The algorithm involv...
Article
Full-text available
The enzymatic conversion of lignocellulosic material to sugars can provide a carbon source for the production of energy (fuels) and a wide range of renewable products. However, the efficiency of this conversion is impaired due to product (sugar) inhibition. Even though several studies investigate how to overcome this challenge, concepts on the proc...
Article
A sustainable management of environment and agriculture is crucial to protect soil, water, and air during intensified agriculture practices as well as huge industrial and transportation activities. A promising tool to address these challenges could be the application of biochar, a carbonaceous product of biomass pyrolysis. The efficiency of biochar...
Article
Full-text available
Simultaneous saccharification and fermentation (SSF) at high solid loading is a potential approach to improve the economic feasibility of cellulosic ethanol. In this study, SSF using high loading of rice straw was assessed using a vertical ball mill reactor. First, the conditions of temperature and number of glass spheres were optimized at 8% (w/v)...
Article
Full-text available
This study assessed the feasibility of using bleached cellulose pulp from Eucalyptus wood as a feedstock for the production of itaconic acid by fermentation. Additionally, different process strategies were tested with the aim of selecting suitable conditions for an efficient production of itaconic acid by the fungus Aspergillus terreus. The feasibi...
Article
Feasible bioprocessing of lignocellulosic biomass requires the use of microbial strains with tolerance to inhibitor compounds and osmotic pressure, able to provide high product yield and productivity. In this sense, this study evaluated the potential of two non-conventional yeasts, Hansenula polymorpha CBS 4732 and Debaryomyces hansenii CBS 767, fo...
Article
Full-text available
This study aimed to evaluate the synthesis and application of heterogeneous catalysts based on heteropolyacids for 5-hydroxymethylfurfural (HMF) production from glucose. Initially, assays were carried out in order to establish the most favorable catalyst synthesis conditions. For such purpose, calcination temperature (300 or 500 °C), type of suppor...
Article
Advanced biorefineries, which aim at valorizing biomass (from agriculture, forestry, aquaculture, among others) into a wide spectrum of products and bioenergy, are seen today as key to implement a sustainable biobased economy. Although different concepts of biorefinery are currently under development, further research and improvement are still requ...
Chapter
A rising number of diabetes patients and the urgent need for novel biotechnological process solutions are the instigation for this work. A biorefinery concept based on the valorization of the hemicellulosic sugar fraction is proposed by producing the sugar substitute xylitol. Its process design is supposed to be performed via superstructure optimiz...
Article
The hydrothermal pretreatment route is gaining research interest as a potentially green method for decon-structing lignocellusic biomass. Based on the relevant literature, the conversion of biomass into platform chemicals or energy carriers through hydrothermal processes has been found to be advantageous by reason of enhanced process performance, w...
Article
This work aimed to support the development of a new process for the production of lipids and carotenoids by oleaginous yeasts using both cellulosic and hemicellulosic fractions of biomass as feedstock for fermentation. The hypothesis behind this idea is that this strategy could lead to a sustainable pathway to produce these valuable components with...
Article
The present study uncovers the in-vitro cytotoxicity, sensing properties, catalytic and antibacterial activity of lignin functionalized silver nanoparticles (AGNPs). Fabricated AGNPs were characterized using UV–vis spectroscopy, particle size, X-ray diffraction (XRD) and Transmission Electron Microscopic (TEM) analyses. The typical surface plasmon...
Article
Four different lignin samples were isolated during the processing of rice straw for ethanol production: one after mild alkaline pretreatment (deacetylation) of rice straw, and other three after simultaneous saccharification and fermentation (fermentation residues), under different process conditions. Then, a complete chemical characterization in te...
Article
Aqueous two-phase systems (ATPS) can be applied to enzymatic reactions that are affected by product inhibition. In the biorefinery context, sugars inhibit the cellulolytic enzymes in charge of converting the biomass. Here, we present a strategy to select an ATPS (formed by polymer and salt) that can separate sugar and enzymes. This automated and mi...
Article
The objective of this work was to study the valorization of residual biomass and wastewater from açai fruit processing to obtain energy products and higher added value from such residues, through the integration between subcritical waterpretreatment and anaerobic digestion technologies. Subcritical watertechnology was performed to pretreat the resi...
Article
Switchgrass (Panicum virgatum) is a perennial grass highly valued as an energy crop resource for the production of bioethanol due to its high carbohydrate content, fast growth, and ability to grow in lands that cannot support crop or food production. In the present study, this biomass was submitted to steam explosion pretreatment in a semi-continuo...
Chapter
Biotransformation of sucrose to fructooligosaccharides (FOS) was investigated using the catalytic action of fructosyltransferase (FFase) originated from solid-state fermentation of agro-industrial wastes (sugarcane bagasse, sotol bagasse, Agave fibers, and polyurethane) using four fungal strains (Aspergillus niger GH1, A. niger PSH, Penicillium cit...
Article
Full-text available
This work describes a new process for the production of beverages from spent coffee grounds (SCG), as well as the chemical and sensory profiles. The process consisted of the extraction of antioxidant phenolic compounds of SCG, followed by the fermentation of this extract supplemented with sucrose and fermented broth distillation. Thus, two fermente...
Article
Brewer´s spent grain (BSG) is an agro-industrial residue rich in proteins, which correspond to approximately 18–30% (w/w) of its composition in a dry weight basis. In the present study, different pretreatment strategies including alkaline, acid, enzymatic and hydrothermal, and their combinations, were evaluated and compared with the aim of extracti...
Article
Fructooligosaccharides (FOS) are important ingredients in the functional food industry because they have different biological properties such as decrease level of triglycerides, cholesterol and phospholipids and stimulate growth of probiotics for enhancement of microflora in large intestine. However, current strategies for the FOS production throug...
Article
In the present study, poly(vinyl alcohol) (PVA) - Acacia lignin (PL) nanofiber mats loaded with silver nanoparticles were prepared and characterized. In-situ synthesis of silver nanoparticles was accomplished using alkali lignin extracted from Acacia wood as a reducing agent. Ultrafine nanofiber mats of PL incorporated with silver nanoparticles wer...
Article
Full-text available
The start-up conditions of mesophilic anaerobic co-digestion of restaurant food waste and vinasse, a waste from sugarcane industry, was investigated for efficient biogas production. A pilot plant, containing two reactors, was designed and used sequentially and semi-continuously for biogas production. All effective operational parameters were contro...
Article
Full-text available
The technology of anaerobic digestion allows the use of biodegradable waste for energy production by breaking down organic matter through a series of biochemical reactions. Such process generates biogas (productivity of 0.45 Nm³/KgSV), which can be used as energy source in industrial activities or as fuel for automotive vehicles. Anaerobic digestio...
Chapter
The aim of this chapter is to present a short critical overview on the various municipal solid waste management (MSWM) technologies together with a glance at how conducting a feasibility study to choose the most suitable scenario for a particular region. In the first part, a conceptual approach to waste management followed by a valuable laconic rev...
Article
Full-text available
This paper deals with the characterization of brewer’s spent grain (BSG) and the optimization of the phosphoric acid pretreatment for this feedstock. The influence of temperature and acid concentration on BSG was studied and the optimal conditions were found to be 155 ºC and 2% H3PO4. The use of both pretreatment and enzymatic hydrolysis together r...
Article
Sugarcane bagasse (SCB) hydrolysate could be an interesting source for red pigment production by Monascus ruber Tieghem IOC 2225. The influence of different wavelength of light-emitting diode (LED) at 250 μmol.m-2.s-1 of photon flux density on red pigment production by M. ruber in glucose-based medium was evaluated. Then, SCB hydrolysate was used a...