Sofia Morais

Sofia Morais
LUCTA S.A. · Innovation Division

PhD

About

115
Publications
29,909
Reads
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4,794
Citations
Citations since 2017
18 Research Items
2286 Citations
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20172018201920202021202220230100200300400
20172018201920202021202220230100200300400
20172018201920202021202220230100200300400
Additional affiliations
July 2011 - December 2014
IRTA Institute of Agrifood Research and Technology
Position
  • Researcher
July 2007 - June 2011
University of Stirling
Position
  • PostDoc Position
February 2006 - January 2007
Université Bordeaux 1
Position
  • PostDoc Position

Publications

Publications (115)
Article
Using rainbow trout (Oncorhynchus mykiss) as a model, we aimed to obtain information about the gastrointestinal tract (GIT) amino acid sensing capacity and hormone production along regions of the GIT, in response to proline (Pro), to a solution of free amino acids (FAA) mimicking the composition of a fishmeal (FM) aqueous extract (FMFAA), or to the...
Article
Full-text available
In this study, we investigated the transcriptional spatio-temporal dynamics of the taste 1 receptor (T1R) gene family repertoire in seabream (Sparus aurata [sa]), during larval ontogeny and in adult tissues. In early larval development, saT1R expression arises heterochronously, i.e. the extraoral taste-related perception in the gastrointestinal tra...
Article
Sow feed intake during lactation is crucial to the maintenance of sow body condition, milk production, and litter performance. Loss of body weight or condition during this time could compromise future reproductive performance. Two groups (n = 52 total) of breeding age gilts and sows were used to evaluate whether a sensory additive would improve int...
Article
Full-text available
This study aimed to characterize amino acid sensing systems in the gastrointestinal tract (GIT) of the carnivorous fish model species rainbow trout. We observed that the trout GIT expresses mRNAs encoding some amino acid receptors described in mammals [calcium-sensing receptor (CaSR), G protein-coupled receptor family C group 6 member A (GPRC6A), a...
Article
Full-text available
A plethora of molecular and functional studies in tetrapods has led to the discovery of multiple taste 1 receptor (T1R) genes encoding G-protein coupled receptors (GPCRs) responsible for sweet (T1R2 + T1R3) and umami (T1R1 + T1R3) taste. In fish, the T1R gene family repertoires greatly expanded because of several T1R2 gene duplications, and recent...
Article
To assess the putative role of taste and pre-absorptive sensing of amino acids in food intake control in fish, we carried out an oral administration with L-leucine, L-valine, L-proline or L-glutamic acid in rainbow trout (Oncorhynchus mykiss). Treatment with proline significantly reduced voluntary food intake at 2h and 3h after oral administration,...
Article
Full-text available
Very long-chain fatty acids (VLC-FA) play critical roles in neural tissues during the early development of vertebrates. However, studies on VLC-FA in fish are scarce. The biosynthesis of VLC-FA is mediated by elongation of very long-chain fatty acid 4 (Elovl4) proteins and, consequently, the complement and activity of these enzymes determines the c...
Article
Full-text available
Long-chain polyunsaturated fatty acids (LC-PUFA) have been extensively studied in aquaculture due to their importance for survival and development in teleosts. However, very long-chain polyunsaturated fatty acids (VLC-PUFA) have been practically unexplored within the aquaculture scenario. VLC-PUFA, although always present in small amounts, can be p...
Article
Expression of genes related to long-chain (C18-22) and very long-chain (>C24) fatty acid biosynthesis in gilthead seabream (Sparus aurata) and Senegalese sole (Solea senegalensis) larvae: Investigating early ontogeny and nutritional regulation Please cite this article as: M. Torres, J.C. Navarro, I. Varó, et al., Expression of genes related to long...
Article
Full-text available
Squid visceral paste is a popular attractant and feeding stimulator supplement in shrimp feeds in China, but it often faces problems with fluctuating availability, cost, composition, and quality. An 8‐week experiment was performed to test a proprietary palatability enhancer as an alternative to squid paste in white shrimp feed. Seven diets were tes...
Article
Full-text available
To continue gathering knowledge on the central regulation of food intake in response to amino acids in teleost fish, using as a model rainbow trout (Oncorhynchus mykiss), we evaluated in a first experiment the feeding attractiveness of L-leucine, L-valine, and L-proline offered as an agar gel matrix. In a second experiment, we assessed the effect o...
Article
Umami in pigs is a taste that helps the animal to identify protein sources through the stimulation of the pT1R1/pT1R3 receptor present in oral tissues and along the gastrointestinal tract, where it acts as a nutrient sensor. Performance benefits have been previously described with the use of a high-intensity umami additive (HIU) based on Maillard r...
Article
To assess the existence of central amino acid sensing systems in fish we carried out two experiments in rainbow trout. In the first one, we injected ICV two different branched-chain amino acids (BCAA), leucine and valine, and assessed food intake up to 48 h later. Leucine decreased and valine increased food intake. In a second experiment, 6h after...
Article
Vegetable oils (VO) are increasingly used to substitute fish oil (FO) in aquafeeds in order to meet demands of the rapidly growing aquaculture industry. However, effects of dietary VO on appetite and food intake have hardly been investigated, despite the importance of these factors for determining growth and body composition of cultured fish. This...
Article
Studies in fish have reported the presence and function of fatty acid (FA)-sensing systems comparable in many aspects to those known in mammals. Such studies were carried out in juvenile and adult fish, but the presence of FA sensing systems and control of food intake has never been evaluated in early stages, despite the importance of establishing...
Article
Full-text available
Recent advances in understanding the molecular basis of taste physiology in fish could open new opportunities to optimize feeding performance in aquaculture. This is particularly relevant at a time when alternative ingredients are being increasingly used, often reducing the digestibility and acceptability of fish diets, even if they are nutritional...
Article
Little is known how dietary lipids affect food intake during larval development of fish, especially with regard to fatty acid (FA) composition. In fact, very little work has been done on appetite regulation and food intake in fish larvae in general, due to biological and technical difficulties associated with this type of studies. A new method usin...
Article
To date, most research on larval lipid nutrition has been centered on essential fatty acid requirements. However, less is known about effects of dietary fatty acid (FA) composition on lipid absorption and metabolism, as addressed in this study. Senegalese sole were fed live preys enriched with different oils: cod liver oil (CLO), linseed oil (LSO),...
Article
Full-text available
Salmons raised in aquaculture farms around the world are increasingly subjected to sub-optimal environmental conditions, such as high water temperatures during summer seasons. Aerobic scope increases and lipid metabolism changes are known plasticity responses of fish for a better acclimation to high water temperature. The present study aimed at inv...
Article
This study analyses the effects of dietary lipid level and source on lipid absorption and metabolism in Senegalese sole (Solea senegalensis). Juvenile fish were fed 4 experimental diets containing either 100 % fish oil (FO) or 25 % FO and 75 % vegetable oil (VO; rapeseed, linseed and soybean oils) at two lipid levels (~8 or ~18 %). Effects were ass...
Article
We assessed the presence of fatty acid (FA) sensing mechanisms in hypothalamus of Senegalese sole (Solea senegalensis) and investigated their sensitivity to FA chain length and/or level of unsaturation. Stearate (SA, saturated FA), oleate (OA, monounsaturated FA of the same chain length), α-linolenate (ALA, a n-3 PUFA of the same chain length), and...
Article
The present study presents the first “in vivo” evidence of enzymatic activity and nutritional regulation of a Δ4-desaturase-dependent DHA synthesis pathway in the teleost Solea senegalensis. Juvenile fish were fed diets containing 2 lipid levels (8 and 18%, LL and HL) with either 100% fish oil (FO) or 75% of the FO replaced by vegetable oils (VOs)....
Article
Full-text available
Senegalese sole was one of the earliest identified candidate species with high potential for aquaculture diversification in the south of Europe. Its culture has been possible, and commercially attempted, for several decades, but intensive production has been slow to take off. This has been explained mostly by serious disease problems, high mortalit...
Article
Senegalese sole is considered a species with high interest for aquaculture diversification in the South of Europe whose production has shown an important increase in recent years. However, broodstock nutrition of this species is still based on a diet of fresh feeds, which poses important sanitary risks, has variable supply and nutritional compositi...
Conference Paper
Long chain polyunsaturated fatty acids are biologically active, with arachidonic acid (20:4n-6, ARA) and eicosapentaenoic acid (20:5n-3, EPA) being the two major omega-6 and omega-3 counterparts, respectively. The enzyme cyclooxygenase (COX-2) and 5-Lypooxygenase (ALOX-5) bioconvert ARA into the commonly termed "inflammatory eicosanoids", including...
Article
Full-text available
Two surveys of over 1,700 publications whose authors use quantitative real-time PCR (qPCR) reveal a lack of transparent and comprehensive reporting of essential technical information. Reporting standards are significantly improved in publications that cite the Minimum Information for Publication of Quantitative Real-Time PCR Experiments (MIQE) guid...
Article
Full-text available
Two surveys of over 1,700 publications whose authors use quantitative real-time PCR (qPCR) reveal a lack of transparent and comprehensive reporting of essential technical information. Reporting standards are significantly improved in publications that cite the Minimum Information for Publication of Quantitative Real-Time PCR Experiments (MIQE) guid...
Article
Full-text available
Dietary fatty acids, particularly arachidonic acid (ARA), affect cortisol and may influence the expression of genes involved in stress response in fish. The involvement of ARA on stress, lipid, and eicosanoid metabolism genes, in Senegalese sole, was tested. Post-larvae were fed Artemia presenting graded ARA levels (0.1, 0.4, 0.8, 1.7, and 2.3 %, d...
Article
Full-text available
Heart and Skeletal Muscle Inflammation (HSMI) is an emerging viral disease caused by a novel Atlantic salmon reovirus (ASRV) affecting farmed fish. Primary symptoms associated with HSMI include myocardial and skeletal muscle necrosis indicating a severe inflammatory process. Recently, we applied the concept of clinical nutrition to moderate the lon...
Article
Fish larvae are astonishing organisms. In spite of being considered the smallest free-living vertebrates that hatch with a very simple digestive system, they show a remarkable efficiency with regards to vital processes like feeding behavior (prey detection, capture and ingestion) and food digestion (enzymatic digestion and neuroendocrine control of...
Article
Full-text available
Previous studies have shown higher levels of arachidonic acid (20:4n-6, ARA) in testis, liver, and muscle of wild Senegalese sole (Solea senegalensis) compared to fish reared in captivity (first generation, G1). The present study was conducted to establish the optimal level of dietary ARA for G1 Senegalese sole broodstock, using as a reference the...
Article
Full-text available
Heart and Skeletal Muscle Inflammation (HSMI), recently associated with a novel Atlantic salmon reovirus (ASRV), is currently one of the most prevalent inflammatory diseases in commercial Atlantic salmon farms in Norway. Mortality varies from low to 20%, but morbidity can be very high, reducing growth performance and causing considerable financial...
Data
Normalized gene expression levels in heart for different gene groups. The genes listed in Tables 4–9 are included in figures A–F, showing the average gene expression levels (as whiskers), with maximum and minimum range for each gene group. Outliers are depicted as black dots. A) Viral infection-related genes; B) Innate immune system-related genes;...
Data
Complete list of the down-regulated genes at 12wpc on the group of fish fed with the ST diet and 16wpc on the group of fish fed with the FF2 diet from cluster groups (identified by SOM). (XLSX)
Data
Complete list of the down-regulated genes at 12wpc on the group of fish fed with the ST diet and 16wpc on the group of fish fed with the FF2 diet from cluster groups (identified by SOM). (XLSX)
Data
Criteria used to score the histological changes in the heart (epicard, ventricle and atrium). (DOCX)
Data
Primers used for RT-qPCR analyses. (DOCX)
Article
Full-text available
Dietary fatty acid supply can affect stress response in fish during early development. Although knowledge on the mechanisms involved in fatty acid regulation of stress tolerance is scarce, it has often been hypothesised that eicosanoid profiles can influence cortisol production. Genomic cortisol actions are mediated by cytosolic receptors which may...
Article
Full-text available
Expansion of aquaculture requires alternative feeds and breeding strategies to reduce dependency on fish oil (FO) and better utilization of dietary vegetable oil (VO). Despite the central role of intestine in maintaining body homeostasis and health, its molecular response to replacement of dietary FO by VO has been little investigated. This study e...
Article
Full-text available
Genetic selection of Atlantic salmon families better adapted to alternative feed formulations containing high levels of vegetable ingredients has been suggested to ensure sustainable growth of aquaculture. The present study aimed to identify molecular pathways that could underlie phenotypic differences in flesh n-3 long-chain polyunsaturated fatty...
Data
Figure S1. Relationship between total lipid level and n-3 LC-PUFA content.
Data
Table S2. Genes similarly regulated in pair-wise comparisons of families containing H and L n-3 LC-PUFA flesh contents, at each one of the total lipid levels.
Data
Table S1. Gene Ontology terms showing significant enrichment in the list of features affected by the 'total lipid' factor.
Data
Figure S2. Distribution of IPN resistance scores in relation to flesh lipid phenotypes.
Article
Solea senegalensis is an unusual marine teleost as it has very low dietary requirement for long-chain polyunsaturated fatty acids (LC-PUFA) during early development. Aquaculture is rapidly becoming the main source of health-beneficial fish products for human consumption. This, associated with limited supply of LC-PUFA-rich ingredients for fish feed...
Article
For aquaculture of marine species to continue to expand, dietary fish oil (FO) must be replaced with more sustainable vegetable oil (VO) alternatives. Most VO are rich in n-6 polyunsaturated fatty acids (PUFA) and few are rich in n-3 PUFA but Camelina oil (CO) is unique in that, besides high 18:3n-3 and n-3/n-6 PUFA ratio, it also contains substant...
Article
Full-text available
The present study investigates the effects of genotype on responses to alternative feeds in Atlantic salmon. Microarray analysis of the liver transcriptome of two family groups, lean or fat, fed a diet containing either a fish oil (FO) or a vegetable oil (VO) blend indicated that pathways of cholesterol and lipoprotein metabolism might be different...
Article
Full-text available
Expansion of aquaculture is seriously limited by reductions in fish oil (FO) supply for aquafeeds. Terrestrial alternatives such as vegetable oils (VO) have been investigated and recently a strategy combining genetic selection with changes in diet formulations has been proposed to meet growing demands for aquaculture products. This study investigat...
Article
The concentration of n-3 long-chain polyunsaturated fatty acids (LC-PUFA) in neural tissues is known to be crucial for effective prey capture from the time of first feeding in marine fish larvae. Furthermore, tissues of tunids, including Atlantic bluefin tuna, have relatively high levels of DHA (docosahexaenoic acid, 22:6n-3) and a high ratio of DH...
Article
Full-text available
An experiment was conducted in order to evaluate the effects of feeding frozen Artemia diets differing in arachidonic acid-to-eicosapentaenoic acid ratios (ARA/EPA) on growth, survival and stress coping ability of Senegalese sole post-larvae (19-31 days after hatch). Two experimental diets presenting high ('High'; 3.0) or low ('Low'; 0.7) ARA/EPA r...
Article
Neural and alimentary cholecystokinin (CCK) levels in Atlantic herring, Clupea harengus, were analyzed from hatching to 40days after hatching (DAH). The head compartment representing the neural pool was quantitatively dominant (>80% of the total CCK content) while the digestive tract pool represented 6-10%. During ontogeny the CCK level in whole la...
Article
Fish are the primary source in the human food basket of the n-3 long-chain polyunsaturated fatty acids, eicosapentaenoate (EPA; 20:5n-3) and docosahexaenoate (DHA; 22:6n-3), that are crucial to the health of higher vertebrates. Atlantic salmon are able to synthesize EPA and DHA from 18:3n-3 through reactions catalyzed by fatty acyl desaturases (Fad...
Article
Full-text available
Despite the large progress obtained in recent years, Senegalese sole (Solea senegalensis) production of high quality juveniles is still a bottleneck. This paper examines the effect of larval and post-larval lipid nutrition on juvenile performance and quality. Four dietary treatments were tested: A—enriched Artemia spp. (EA); B—non-enriched Artemia...
Article
Full-text available
Despite the recent progress in the production of inert diets for fish larvae, feeding of most species of interest for aquaculture still relies on live feeds during the early life stages. Independently of their nutritional value, live feeds are easily detected and captured, due to their swimming movements in the water column, and highly digestible,...
Article
The aim of this paper is to provide explanations of how dietary phospholipid (PL) globally improves fish larval development, including growth and survival, digestive functions and skeletal development, and to propose optimal PL levels and sources in fish larval diets. Dietary incorporation of 8-12% PL related to dry matter (d.m.) promotes growth an...
Article
In the present paper, we investigated the expression of fatty acyl desaturase and elongase genes in a marine teleost, cobia, a species of great interest due to its considerable aquaculture potential. A cDNA was cloned that, when expressed in yeast, was shown to result in desaturation of 18:3n-3 and 18:2n-6, indicating that it coded for a Δ6 desatur...
Article
The present study intended to evaluate the effects of early introduction of inert diet in lipid digestibility and metabolism of sole, while larval feed intake, growth and survival were also monitored. Solea senegalensis larvae were reared on a standard live feed regime (ST) and co-feeding regime with inert diet (Art R). Trials using sole larvae fed...