Sofia Forssten

Sofia Forssten
International Flavors & Fragrances Inc. | IFF · Health & Biosciences

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64
Publications
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Publications

Publications (64)
Article
Full-text available
There are different models available that mimic the human intestinal epithelium and are thus available for studying probiotic and pathogen interactions in the gastrointestinal tract. Although, in vivo models make it possible to study the overall effects of a probiotic on a living subject, they cannot always be conducted and there is a general commi...
Article
Full-text available
The microbiota–gut–brain axis is a bidirectional communication pathway that enables the gut microbiota to communicate with the brain through direct and indirect signaling pathways to influence brain physiology, function, and even behavior. Research has shown that probiotics can improve several aspects of health by changing the environment within th...
Article
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Aims: Use of non-steroidal anti-inflammatory drugs (NSAIDs) can cause damage to the gastric and duodenal mucosa. Some probiotics have proven useful in ameliorating the harmful side-effects of NSAIDs. Our aim was to evaluate whether oral administration of Bifidobacterium animalis ssp. lactis 420 (B420) can attenuate the increase of calprotectin exc...
Chapter
The intestinal microbiota changes in response to aging. The intestinal microbiota has a profound effect on the functioning of the intestine in health and disease. There are hormonal, immunological and neurological connections between the intestine and other organs systems in the body, and potentially vice versa. The intestinal microbiota may theref...
Article
Full-text available
The successful development of probiotic foods and dietary supplements rests on three pillars; each with their specific challenges and opportunities. First, strain production; this depends on selecting the right strain with promising technological properties and safety profile. Further the manufacturing of the strain in a stable format at sufficient...
Article
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The present study aimed to investigate whether probiotic recovery is affected when consumed together with antibiotics. Fecal samples were collected from an earlier antibiotic associated diarrhea, randomized, placebo-controlled study with a product consisting of a combination of Lactobacillus acidophilus NCFM, Lactobacillus paracasei Lpc-37, and Bif...
Article
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The probiotic definition stipulates “adequate amounts”. Here, we investigated the metabolic output and recovery rate of probiotic strains using a simulated upper gastro-intestinal passage and colonic fermentation. Two different doses, 7 × 109 colony forming units (CFU) and 7 × 1010 CFU, of a probiotic mixture (Bifidobacterium lactis Bl-04, Lactobac...
Article
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The role of nasal and fecal microbiota in viral respiratory infections has not been established. We collected nasal swabs and washes, and fecal samples in a clinical study assessing the effect of probiotic Bifidobacterium animalis subsp. lactis Bl-04 on experimental rhinovirus infection. The nasal and fecal microbiota were characterized by 16S rRNA...
Article
The present study introduces a novel triple-phase (liquids, solids and gases) approach, which employed uniformly labelled [U-13C] polydextrose (PDX) for the selective profiling of metabolites generated from dietary fiber fermentation in an in vitro colon simulator using human fecal inocula. Employing 13C NMR spectroscopy, [U-13C] PDX metabolism was...
Article
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Background: Probiotic formulations can be single- or multi-strain. Commercially, multi-strain preparations have been suggested to have improved functionality over single-strain cultures. Probiotics are often tested as single-strain preparations but may subsequently be commercially formulated as multi-strain products. Objective: The aim of this stud...
Article
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Bacillus spp. are widely used in animal production for their probiotic properties. In many animal species, feed supplementation with specific Bacillus strains can provide numerous benefits including improvement in digestibility, the gut microbiota and immune modulation, and growth performance. Bacilli are fed to animals as spores that can sustain t...
Article
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The aim was to develop novel fibres by enzymatic synthesis, to determine their total dietary fibre by AOAC method 2009.01 and to estimate their potential digestibility and assess their digestibility in vivo using glycaemic and insulinaemic responses as markers in mice and randomised clinical trial models. We found that fibre candidates to which α-(...
Article
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Obesity is related to the consumption of energy-dense foods in addition to changes in the microbiome where a higher abundance of gut Bacteroidetes can be found in lean subjects or after weight loss. Lactitol, a sweet-tasting sugar alcohol, is a common sugar-replacement in foods. Polydextrose, a highly branched glucose polymer, is known to reduce en...
Article
Objectives: In vitro methods to study dental biofilms are useful in finding ways to support a healthy microbial balance in the oral cavity. The effects of sucrose, xylitol, and their combination on three strains of Streptococcus mutans and one strain of Streptococcus sobrinus were studied using a dental simulator. Methods: A simulator was used t...
Article
Probiotics are live microorganisms, mainly belonging to the genera Lactobacillus and Bifidobacterium, although also strain of other species are commercialized, that have a beneficial effect on the host. From the perspective of antibiotic use, probiotics have been observed to reduce the risk of certain infectious disease such as certain types of dia...
Article
Full-text available
Understanding the interaction between the gut microbial activity and the host is essential, and in vitro models are being used to test and develop hypotheses regarding the impact of food components/drugs on the human gut ecosystem. However, while in vitro models provide excellent possibilities for dynamic investigations, studies have commonly been...
Article
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Background: Clostridium difficile is a natural resident of the intestinal microbiota; however, it becomes harmful when the normal intestinal microbiota is disrupted, and overgrowth and toxin production occurs. The toxins can cause bloating and diarrhoea, which may cause severe disease and have the potential to cause outbreaks in hospitals and othe...
Article
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The effects of a high level of dietary fibre (DF) either as arabinoxylan (AX) or resistant starch (RS) on digestion processes, SCFA concentration and pool size in various intestinal segments and on the microbial composition in the faeces were studied in a model experiment with pigs. A total of thirty female pigs (body weight 63·1 (sem 4·4) kg) were...
Article
The aim of the present study was to elucidate the impact of polydextrose (PDX), an insoluble fiber, on the human fecal metabolome by high-resolution nuclear magnetic resonance (NMR) spectroscopy-based metabolomics in a dietary intervention study (n=12). Principal component analysis (PCA) revealed a strong effect of PDX consumption on the fecal meta...
Article
Full-text available
To study the effect of probiotic consumption on the faecal microbiota during and after antibiotic exposure.
Article
Probiotics, prebiotics, and combinations thereof, that is synbiotics, have been reported to modulate gut microbiota of humans. In this study, effects of a novel synbiotic on the composition and metabolic activity of human gut microbiota were investigated. Healthy volunteers (n = 18) were enrolled in a double-blinded, randomized, and placebo-control...
Article
Full-text available
Prebiotics, probiotics and synbiotics are dietary ingredients with the potential to influence health and mucosal and systemic immune function by altering the composition of the gut microbiota. In the present study, a candidate prebiotic (xylo-oligosaccharide, XOS, 8 g/d), probiotic (Bifidobacterium animalis subsp. lactis Bi-07, 109 colony-forming u...
Patent
Full-text available
A feed additive composition comprising a direct fed microbial (DFM), in combination with a xylanase (e.g. endo-1,4-β-d-xyIanase) and a β-glucanase (and optionally a further fibre degrading enzyme), wherein the DFM is selected from the group consisting of an enzyme producing strain; a C5 sugar-fermenting strain; a short-chain fatty acid-producing st...
Article
A Trypanosoma cruzi trans-sialidase (E.C. 3.2.1.18) was cloned into Pichia pastoris and expressed. The pH and temperature optimum of the enzyme was determined as pH 5.7 and 30°C. Using casein glycomacropeptide (CGMP) and lactose as sialyl-donor and acceptor respectively, the optimal donor/acceptor ratio for the trans-sialidase catalysed 3'-sialylla...
Article
Both lactic acid bacteria (LAB) and bacilli are commercialised as probiotics; LAB are mainly utilised as live and active or dormant cells, while bacilli are utilised as spores. Belonging to very different genera, they have different technological properties. LAB have a longer history of use as probiotics and therefore have a broader base as far as...
Article
Full-text available
Elderly adults have alterations in their gut microbiota and immune functions that are associated with higher susceptibility to infections and metabolic disorders. Probiotics and prebiotics, and their synbiotic combinations are food supplements that have been shown to improve both gut and immune function. The objective of this randomised, double-bli...
Article
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To assess the effect of Lactobacillus acidophilus (American Type Culture Collection (ATCC) 700396) on enterotoxigenic Escherichia coli (ETEC) infection, in the present study, a parallel, double-blind, placebo-controlled 4-week intervention was performed in healthy males. The subjects largely consumed their habitual diet, but had to abstain from con...
Article
A negative energy balance can be accomplished by reducing the caloric intake which results in an increased feeling of hunger. This physiological state is regulated by secretion of satiety hormones. The secretion of these hormones can be influenced by ingestion of e.g. fat. Fat, dairy beverage and synbiotic mixture have been found to have satiety-in...
Article
At different stages of life, probiotics can provide health benefits to the consumer. Certain benefits, such as reduction in allergy, are typical for infants. Modulation of immune function and maintenance of the intestinal microbiota are mainly beneficial during infancy/childhood and at old age. Although it may seem that in adulthood, in the prime o...
Article
Background & aims: This study is a part of the clinical trials with probiotic bacterium Lactobacillus salivarius Ls-33 conducted in obese adolescents. Previously reported clinical studies showed no effect of Ls-33 consumption on the metabolic syndrome in the subject group. The aim of the study was to investigate the impact of L. salivarius Ls-33 o...
Article
Dietary fiber and slow carbohydrates can have differential beneficial effects on gut microbial composition and metabolism. Insoluble substrates such as RS cannot be used in continuous simulation systems and we tested an in vitro batch fermentation system for possible prebiotic effects using waxy maize starch granules, pectin-rich potato fiber, and...
Article
Full-text available
Prebiotics, probiotics and synbiotics can be used to modulate both the composition and activity of the gut microbiota and thereby potentially affecting host health beneficially. The aim of this study was to investigate the effects of eight synbiotic combinations on the composition and activity of human fecal microbiota using a four-stage semicontin...
Data
Growth of Lactobacillus acidophilus NCFM (dark grey) and Bifidobacterium animalis subsp. lactis Bl-04 (white) shown as area under the growth curve. Carbohydrates are numbered 1–37 according to Table S1 and listed according to degree of polymerization (DP). The growth of Bl-04 on carbohydrates 4, 34 and 37 was not tested. Glucose and polydextrose (P...
Data
Carbohydrates screened for growth stimulation of Lactobacillus acidophilus NCFM and Bifidobacterium animalis subsp. lactis Bl-04 listed with names and manufacturers. Size is given as degree of polymerization (DP). (DOC)
Data
Concentrations of branched-chain fatty acids as determined by gas chromatography; isobutyric acid (A), 2-methylbutyric acid (B) and isovaleric acid (C) in vessels V1–V4 of the colonic model after control and synbiotic fermentations. Synbiotic fermentations are denoted as follows: Lactobacillus acidophilus NCFM in combination with; isomaltulose ()(n...
Article
Full-text available
To compare quantities of predominant and pathogenic bacteria in mucosal and faecal samples. Twenty patients undergoing diagnostic colonoscopy with endoscopically and histologically normal mucosa were recruited to the study, 14 subjects of which also supplied faecal (F) samples between 15 d to 105 d post colonoscopy. Mucosal biopsies were taken from...
Article
The composition and activity of the intestinal microbiota appears to change upon age as a result of changes in gut physiology, diet, as well as immunological changes. The age-related changes in the composition and activity of the intestinal microbiota seem to correlate with a greater vulnerability to inflammatory and viral disease, and, therefore,...
Article
Specific strains of probiotics can impact on the composition and function of the intestinal bacterial community in the elderly. The consumption of probiotics at a daily dose of 10 9-10 10CFU affects the composition and activity of the intestinal microbiota and may help to maintain a healthy microbiota or improve the levels of beneficial bacteria. P...
Article
Full-text available
In this placebo-controlled, double-blind, crossover human feeding study, the effects of polydextrose (PDX; 8 g/d) on the colonic microbial composition, immune parameters, bowel habits and quality of life were investigated. PDX is a complex glucose oligomer used as a sugar replacer. The main goal of the present study was to identify the microbial gr...
Article
Colonic microbiota is involved in the etiology of colon cancer according to several reports. Studies also indicate that the microbiota differs between atopic patients and healthy subjects. To evaluate whether a probiotic mix containing Lactobacillus paracasei Lpc-37, Lactobacillus acidophilus 74-2, and Bifidobacterium animalis subsp. lactis DGCC 42...
Article
Ageing changes gut microbiota composition and alters immune system function. Probiotics, prebiotics and synbiotics may improve the health status of elderly individuals by modifying the intestinal environment and the microbiota composition, and by stimulating the immune system. In this work, we studied the effects of synbiotic supplementation on the...
Article
Saliva is a complex fluid that possesses many important functions regarding oral health. Many in vitro studies require relatively large quantities of saliva. While natural saliva would be the material of choice, it is difficult to obtain in sufficient quantities and varies in composition. Substitutes mimicking the physicochemical properties of sali...
Article
Probiotic products have gained popularity with consumers that expect that the products they consume are healthy and help them maintain health. Hence, the need and preferences of the consumers are translated into a product format concept. Probiotics have been used for a long time as natural components in supplements and functional foods, mainly in f...
Article
Cheese has been shown to be an appropriate carrier for selected strains of probiotics. The functionality of probiotics in cheese has, however, been less investigated. A model simulating the human gastrointestinal tract showed that L. acidophilus NCFM and L. rhamnosus HN001 in Gouda cheese survived passage through the upper gastrointestinal tract. F...
Article
Full-text available
Aging is associated with alterations in the intestinal microbiota and with immunosenescence. Probiotics have the potential to modify a selected part of the intestinal microbiota as well as improve immune functions and may, therefore, be particularly beneficial to elderly consumers. In this randomized, controlled cross-over clinical trial, we assess...
Chapter
Full-text available
The human microbiota undergoes changes throughout life. From being sterile in a foetus, the gastrointestinal tract of a newborn child is rapidly colonized with bacteria from both the mother and the environment. A less complex microbiota dominates during the first two years of life where after a more complex composition of the microbiota is establis...
Article
Probiotics have been used for about a century as natural components in supplements and functional foods, mainly in milk drinks and yogurts. Selected probiotic strains have also been included into cheese which has been shown to be an appropriate carrier for probiotics and can deliver them in sufficient numbers. The food matrix in which probiotics ar...
Article
Clin Microbiol Infect 2010; 16: 1158–1161 The molecular epidemiology of 33 Escherichia coli and 81 Klebsiella pneumoniae extended-spectrum β-lactamase-producing healthcare-associated and community-acquired isolates collected in the Helsinki district during 2000–2004 was investigated. Clonality studies, antimicrobial susceptibility and genotyping of...
Article
Probiotics are commonly consumed in the form of supplements and fermented (drink) yogurts. However, alternative carriers are possible and especially cheese seems to be well suited. Selected Lactobacillus strains (L. acidophilus NCFM and L. rhamnosus HN001) were observed to grow during ripening and maintain viability throughout 3 months shelf life....
Article
Full-text available
The effects of Lactobacillus acidophilus NCFM™, lactitol, and the combination of lactitol and L. acidophilus NCFM™ were studied with a semi-continuous colon fermentation simulation; consisting of compartments mimicking, ascending, transverse, descending and sigmoid colon and their conditions with faecal inoculation. L. acidophilus NCFM™ was detecte...
Article
Full-text available
Dental caries and dental plaque are among the most common diseases worldwide, and are caused by a mixture of microorganisms and food debris. Specific types of acid-producing bacteria, especially Streptococcus mutans, colonize the dental surface and cause damage to the hard tooth structure in the presence of fermentable carbohydrates e.g., sucrose a...
Article
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A semi-continuous, anaerobic colon simulator, with four vessels mimicking the conditions of the human large intestine, was used to study the fermentation of xylo-oligosaccharides (XOS). Three XOS compounds and a xylan preparation were fermented for 48 hours by human colonic microbes. Fructo-oligosaccharides (FOS) were used as a prebiotic reference....
Article
Full-text available
Synbiotics are used to manipulate the endogenous microbiota, but whether they are more effective than the constituting pro- and prebiotics alone remains to be determined. The objective of this work was to evaluate galacto-oligosaccharide (GOS), polydextrose (PDX) and Bifidobacterium lactis Bi-07 alone and in combinations in a simulated human colon...
Article
In this study, probiotics Lactobacillus acidophilus NCFM and Lactobacillus rhamnosus HN001 in cheese were studied using models simulating the human gastrointestinal tract with the aim of investigating whether the cheese matrix affected the survival and metabolic properties of these probiotic strains. Probiotics in cheese survived in the simulated u...
Article
Clin Microbiol Infect 2010; 16: 1158–1161 The molecular epidemiology of 33 Escherichia coli and 81 Klebsiella pneumoniae extended-spectrum β-lactamase-producing healthcare-associated and community-acquired isolates collected in the Helsinki district during 2000–2004 was investigated. Clonality studies, antimicrobial susceptibility and genotyping of...
Article
Full-text available
In Klebsiella pneumoniae, the cooccurrence of chromosomal and plasmid-mediated beta-lactamases can hinder their accurate molecular detection. We developed a fast and reliable method that allows the typing of isolates carrying more than one SHV gene. The method is based on pyrosequencing the DNA sequence corresponding to amino acid positions 35, 238...

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